Further immunohistochemical characterization of BRD1 a new susceptibility gene for schizophrenia and bipolar affective disorder
Abstract We have recently shown that the gene BRD1 is associated with schizophrenia and bipolar affective disorder and that the BRD1 protein (BRD1) which is expressed in neurons may occur in a short and a long variant. The aim of the study was to generate polyclonal antibodies against new BRD1 epito...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Bjarkam, Carsten Reidies [verfasserIn] Corydon, Thomas J. [verfasserIn] Olsen, Inger Marie L. [verfasserIn] Pallesen, Jonatan [verfasserIn] Nyegaard, Mette [verfasserIn] Fryland, Tue [verfasserIn] Mors, Ole [verfasserIn] Børglum, Anders D. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Anatomy and embryology - Berlin : Springer, 1891, 214(2009), 1 vom: 10. Sept. |
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| Übergeordnetes Werk: |
volume:214 ; year:2009 ; number:1 ; day:10 ; month:09 |
| Links: |
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| DOI / URN: |
10.1007/s00429-009-0219-3 |
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SPR005717604 |
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| 520 | |a Abstract We have recently shown that the gene BRD1 is associated with schizophrenia and bipolar affective disorder and that the BRD1 protein (BRD1) which is expressed in neurons may occur in a short and a long variant. The aim of the study was to generate polyclonal antibodies against new BRD1 epitopes enabling discrimination between the long and short BRD1 variants, and elucidate the BRD1 distribution in several human tissues, including the CNS. Polyclonal rabbit antibodies were raised against three different BRD1 epitopes. One (67) was specific for the long BRD1 variant, whereas the two others (63/64 and 65/66) like the original monoclonal mouse antibody (K22) were predicted to stain both variants. Immunohistochemical staining procedures were subsequently performed on paraffin-embedded human cerebral cortex and microarray slides containing 30 different human tissues. Western blotting confirmed the predicted specificity of the developed antibodies. K22, 63/64 and 65/66 displayed a similar neuronal staining pattern characterized by a distinct but weak nuclear staining, while the surrounding cytoplasm and proximal dendrites were more intensely stained. Interestingly, staining with 67 generated in contrast primarily an intense nuclear staining. The new antibodies resulted, furthermore, in a prominent neuroglial reaction characterized by staining of cell bodies, nuclei and glial processes. The tissue microarray analysis revealed that BRD1 was widely distributed in human tissues. The particular expression profile, e.g., the degree of nuclear and/or cytoplasmatic staining, seemed, however, to be highly tissue dependent. These results suggest a general role of BRD1 in the cell and stress that the two BRD1 variants may play different roles in the etiology of psychiatric disease. | ||
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| 700 | 1 | |a Børglum, Anders D. |e verfasserin |4 aut | |
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10.1007/s00429-009-0219-3 doi (DE-627)SPR005717604 (SPR)s00429-009-0219-3-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Bjarkam, Carsten Reidies verfasserin aut Further immunohistochemical characterization of BRD1 a new susceptibility gene for schizophrenia and bipolar affective disorder 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We have recently shown that the gene BRD1 is associated with schizophrenia and bipolar affective disorder and that the BRD1 protein (BRD1) which is expressed in neurons may occur in a short and a long variant. The aim of the study was to generate polyclonal antibodies against new BRD1 epitopes enabling discrimination between the long and short BRD1 variants, and elucidate the BRD1 distribution in several human tissues, including the CNS. Polyclonal rabbit antibodies were raised against three different BRD1 epitopes. One (67) was specific for the long BRD1 variant, whereas the two others (63/64 and 65/66) like the original monoclonal mouse antibody (K22) were predicted to stain both variants. Immunohistochemical staining procedures were subsequently performed on paraffin-embedded human cerebral cortex and microarray slides containing 30 different human tissues. Western blotting confirmed the predicted specificity of the developed antibodies. K22, 63/64 and 65/66 displayed a similar neuronal staining pattern characterized by a distinct but weak nuclear staining, while the surrounding cytoplasm and proximal dendrites were more intensely stained. Interestingly, staining with 67 generated in contrast primarily an intense nuclear staining. The new antibodies resulted, furthermore, in a prominent neuroglial reaction characterized by staining of cell bodies, nuclei and glial processes. The tissue microarray analysis revealed that BRD1 was widely distributed in human tissues. The particular expression profile, e.g., the degree of nuclear and/or cytoplasmatic staining, seemed, however, to be highly tissue dependent. These results suggest a general role of BRD1 in the cell and stress that the two BRD1 variants may play different roles in the etiology of psychiatric disease. Human (dpeaa)DE-He213 Psychiatry (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 Chromosome 22 (dpeaa)DE-He213 Tissue microarray (dpeaa)DE-He213 Corydon, Thomas J. verfasserin aut Olsen, Inger Marie L. verfasserin aut Pallesen, Jonatan verfasserin aut Nyegaard, Mette verfasserin aut Fryland, Tue verfasserin aut Mors, Ole verfasserin aut Børglum, Anders D. verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 214(2009), 1 vom: 10. Sept. (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:214 year:2009 number:1 day:10 month:09 https://dx.doi.org/10.1007/s00429-009-0219-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 214 2009 1 10 09 |
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10.1007/s00429-009-0219-3 doi (DE-627)SPR005717604 (SPR)s00429-009-0219-3-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Bjarkam, Carsten Reidies verfasserin aut Further immunohistochemical characterization of BRD1 a new susceptibility gene for schizophrenia and bipolar affective disorder 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We have recently shown that the gene BRD1 is associated with schizophrenia and bipolar affective disorder and that the BRD1 protein (BRD1) which is expressed in neurons may occur in a short and a long variant. The aim of the study was to generate polyclonal antibodies against new BRD1 epitopes enabling discrimination between the long and short BRD1 variants, and elucidate the BRD1 distribution in several human tissues, including the CNS. Polyclonal rabbit antibodies were raised against three different BRD1 epitopes. One (67) was specific for the long BRD1 variant, whereas the two others (63/64 and 65/66) like the original monoclonal mouse antibody (K22) were predicted to stain both variants. Immunohistochemical staining procedures were subsequently performed on paraffin-embedded human cerebral cortex and microarray slides containing 30 different human tissues. Western blotting confirmed the predicted specificity of the developed antibodies. K22, 63/64 and 65/66 displayed a similar neuronal staining pattern characterized by a distinct but weak nuclear staining, while the surrounding cytoplasm and proximal dendrites were more intensely stained. Interestingly, staining with 67 generated in contrast primarily an intense nuclear staining. The new antibodies resulted, furthermore, in a prominent neuroglial reaction characterized by staining of cell bodies, nuclei and glial processes. The tissue microarray analysis revealed that BRD1 was widely distributed in human tissues. The particular expression profile, e.g., the degree of nuclear and/or cytoplasmatic staining, seemed, however, to be highly tissue dependent. These results suggest a general role of BRD1 in the cell and stress that the two BRD1 variants may play different roles in the etiology of psychiatric disease. Human (dpeaa)DE-He213 Psychiatry (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 Chromosome 22 (dpeaa)DE-He213 Tissue microarray (dpeaa)DE-He213 Corydon, Thomas J. verfasserin aut Olsen, Inger Marie L. verfasserin aut Pallesen, Jonatan verfasserin aut Nyegaard, Mette verfasserin aut Fryland, Tue verfasserin aut Mors, Ole verfasserin aut Børglum, Anders D. verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 214(2009), 1 vom: 10. Sept. (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:214 year:2009 number:1 day:10 month:09 https://dx.doi.org/10.1007/s00429-009-0219-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 214 2009 1 10 09 |
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10.1007/s00429-009-0219-3 doi (DE-627)SPR005717604 (SPR)s00429-009-0219-3-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Bjarkam, Carsten Reidies verfasserin aut Further immunohistochemical characterization of BRD1 a new susceptibility gene for schizophrenia and bipolar affective disorder 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We have recently shown that the gene BRD1 is associated with schizophrenia and bipolar affective disorder and that the BRD1 protein (BRD1) which is expressed in neurons may occur in a short and a long variant. The aim of the study was to generate polyclonal antibodies against new BRD1 epitopes enabling discrimination between the long and short BRD1 variants, and elucidate the BRD1 distribution in several human tissues, including the CNS. Polyclonal rabbit antibodies were raised against three different BRD1 epitopes. One (67) was specific for the long BRD1 variant, whereas the two others (63/64 and 65/66) like the original monoclonal mouse antibody (K22) were predicted to stain both variants. Immunohistochemical staining procedures were subsequently performed on paraffin-embedded human cerebral cortex and microarray slides containing 30 different human tissues. Western blotting confirmed the predicted specificity of the developed antibodies. K22, 63/64 and 65/66 displayed a similar neuronal staining pattern characterized by a distinct but weak nuclear staining, while the surrounding cytoplasm and proximal dendrites were more intensely stained. Interestingly, staining with 67 generated in contrast primarily an intense nuclear staining. The new antibodies resulted, furthermore, in a prominent neuroglial reaction characterized by staining of cell bodies, nuclei and glial processes. The tissue microarray analysis revealed that BRD1 was widely distributed in human tissues. The particular expression profile, e.g., the degree of nuclear and/or cytoplasmatic staining, seemed, however, to be highly tissue dependent. These results suggest a general role of BRD1 in the cell and stress that the two BRD1 variants may play different roles in the etiology of psychiatric disease. Human (dpeaa)DE-He213 Psychiatry (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 Chromosome 22 (dpeaa)DE-He213 Tissue microarray (dpeaa)DE-He213 Corydon, Thomas J. verfasserin aut Olsen, Inger Marie L. verfasserin aut Pallesen, Jonatan verfasserin aut Nyegaard, Mette verfasserin aut Fryland, Tue verfasserin aut Mors, Ole verfasserin aut Børglum, Anders D. verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 214(2009), 1 vom: 10. Sept. (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:214 year:2009 number:1 day:10 month:09 https://dx.doi.org/10.1007/s00429-009-0219-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 214 2009 1 10 09 |
| allfieldsGer |
10.1007/s00429-009-0219-3 doi (DE-627)SPR005717604 (SPR)s00429-009-0219-3-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Bjarkam, Carsten Reidies verfasserin aut Further immunohistochemical characterization of BRD1 a new susceptibility gene for schizophrenia and bipolar affective disorder 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We have recently shown that the gene BRD1 is associated with schizophrenia and bipolar affective disorder and that the BRD1 protein (BRD1) which is expressed in neurons may occur in a short and a long variant. The aim of the study was to generate polyclonal antibodies against new BRD1 epitopes enabling discrimination between the long and short BRD1 variants, and elucidate the BRD1 distribution in several human tissues, including the CNS. Polyclonal rabbit antibodies were raised against three different BRD1 epitopes. One (67) was specific for the long BRD1 variant, whereas the two others (63/64 and 65/66) like the original monoclonal mouse antibody (K22) were predicted to stain both variants. Immunohistochemical staining procedures were subsequently performed on paraffin-embedded human cerebral cortex and microarray slides containing 30 different human tissues. Western blotting confirmed the predicted specificity of the developed antibodies. K22, 63/64 and 65/66 displayed a similar neuronal staining pattern characterized by a distinct but weak nuclear staining, while the surrounding cytoplasm and proximal dendrites were more intensely stained. Interestingly, staining with 67 generated in contrast primarily an intense nuclear staining. The new antibodies resulted, furthermore, in a prominent neuroglial reaction characterized by staining of cell bodies, nuclei and glial processes. The tissue microarray analysis revealed that BRD1 was widely distributed in human tissues. The particular expression profile, e.g., the degree of nuclear and/or cytoplasmatic staining, seemed, however, to be highly tissue dependent. These results suggest a general role of BRD1 in the cell and stress that the two BRD1 variants may play different roles in the etiology of psychiatric disease. Human (dpeaa)DE-He213 Psychiatry (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 Chromosome 22 (dpeaa)DE-He213 Tissue microarray (dpeaa)DE-He213 Corydon, Thomas J. verfasserin aut Olsen, Inger Marie L. verfasserin aut Pallesen, Jonatan verfasserin aut Nyegaard, Mette verfasserin aut Fryland, Tue verfasserin aut Mors, Ole verfasserin aut Børglum, Anders D. verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 214(2009), 1 vom: 10. Sept. (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:214 year:2009 number:1 day:10 month:09 https://dx.doi.org/10.1007/s00429-009-0219-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 214 2009 1 10 09 |
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10.1007/s00429-009-0219-3 doi (DE-627)SPR005717604 (SPR)s00429-009-0219-3-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Bjarkam, Carsten Reidies verfasserin aut Further immunohistochemical characterization of BRD1 a new susceptibility gene for schizophrenia and bipolar affective disorder 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We have recently shown that the gene BRD1 is associated with schizophrenia and bipolar affective disorder and that the BRD1 protein (BRD1) which is expressed in neurons may occur in a short and a long variant. The aim of the study was to generate polyclonal antibodies against new BRD1 epitopes enabling discrimination between the long and short BRD1 variants, and elucidate the BRD1 distribution in several human tissues, including the CNS. Polyclonal rabbit antibodies were raised against three different BRD1 epitopes. One (67) was specific for the long BRD1 variant, whereas the two others (63/64 and 65/66) like the original monoclonal mouse antibody (K22) were predicted to stain both variants. Immunohistochemical staining procedures were subsequently performed on paraffin-embedded human cerebral cortex and microarray slides containing 30 different human tissues. Western blotting confirmed the predicted specificity of the developed antibodies. K22, 63/64 and 65/66 displayed a similar neuronal staining pattern characterized by a distinct but weak nuclear staining, while the surrounding cytoplasm and proximal dendrites were more intensely stained. Interestingly, staining with 67 generated in contrast primarily an intense nuclear staining. The new antibodies resulted, furthermore, in a prominent neuroglial reaction characterized by staining of cell bodies, nuclei and glial processes. The tissue microarray analysis revealed that BRD1 was widely distributed in human tissues. The particular expression profile, e.g., the degree of nuclear and/or cytoplasmatic staining, seemed, however, to be highly tissue dependent. These results suggest a general role of BRD1 in the cell and stress that the two BRD1 variants may play different roles in the etiology of psychiatric disease. Human (dpeaa)DE-He213 Psychiatry (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 Chromosome 22 (dpeaa)DE-He213 Tissue microarray (dpeaa)DE-He213 Corydon, Thomas J. verfasserin aut Olsen, Inger Marie L. verfasserin aut Pallesen, Jonatan verfasserin aut Nyegaard, Mette verfasserin aut Fryland, Tue verfasserin aut Mors, Ole verfasserin aut Børglum, Anders D. verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 214(2009), 1 vom: 10. Sept. (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:214 year:2009 number:1 day:10 month:09 https://dx.doi.org/10.1007/s00429-009-0219-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 214 2009 1 10 09 |
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Enthalten in Anatomy and embryology 214(2009), 1 vom: 10. Sept. volume:214 year:2009 number:1 day:10 month:09 |
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Bjarkam, Carsten Reidies @@aut@@ Corydon, Thomas J. @@aut@@ Olsen, Inger Marie L. @@aut@@ Pallesen, Jonatan @@aut@@ Nyegaard, Mette @@aut@@ Fryland, Tue @@aut@@ Mors, Ole @@aut@@ Børglum, Anders D. @@aut@@ |
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Bjarkam, Carsten Reidies |
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610 ASE 44.34 bkl Further immunohistochemical characterization of BRD1 a new susceptibility gene for schizophrenia and bipolar affective disorder Human (dpeaa)DE-He213 Psychiatry (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 Chromosome 22 (dpeaa)DE-He213 Tissue microarray (dpeaa)DE-He213 |
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further immunohistochemical characterization of brd1 a new susceptibility gene for schizophrenia and bipolar affective disorder |
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Further immunohistochemical characterization of BRD1 a new susceptibility gene for schizophrenia and bipolar affective disorder |
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Abstract We have recently shown that the gene BRD1 is associated with schizophrenia and bipolar affective disorder and that the BRD1 protein (BRD1) which is expressed in neurons may occur in a short and a long variant. The aim of the study was to generate polyclonal antibodies against new BRD1 epitopes enabling discrimination between the long and short BRD1 variants, and elucidate the BRD1 distribution in several human tissues, including the CNS. Polyclonal rabbit antibodies were raised against three different BRD1 epitopes. One (67) was specific for the long BRD1 variant, whereas the two others (63/64 and 65/66) like the original monoclonal mouse antibody (K22) were predicted to stain both variants. Immunohistochemical staining procedures were subsequently performed on paraffin-embedded human cerebral cortex and microarray slides containing 30 different human tissues. Western blotting confirmed the predicted specificity of the developed antibodies. K22, 63/64 and 65/66 displayed a similar neuronal staining pattern characterized by a distinct but weak nuclear staining, while the surrounding cytoplasm and proximal dendrites were more intensely stained. Interestingly, staining with 67 generated in contrast primarily an intense nuclear staining. The new antibodies resulted, furthermore, in a prominent neuroglial reaction characterized by staining of cell bodies, nuclei and glial processes. The tissue microarray analysis revealed that BRD1 was widely distributed in human tissues. The particular expression profile, e.g., the degree of nuclear and/or cytoplasmatic staining, seemed, however, to be highly tissue dependent. These results suggest a general role of BRD1 in the cell and stress that the two BRD1 variants may play different roles in the etiology of psychiatric disease. |
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Abstract We have recently shown that the gene BRD1 is associated with schizophrenia and bipolar affective disorder and that the BRD1 protein (BRD1) which is expressed in neurons may occur in a short and a long variant. The aim of the study was to generate polyclonal antibodies against new BRD1 epitopes enabling discrimination between the long and short BRD1 variants, and elucidate the BRD1 distribution in several human tissues, including the CNS. Polyclonal rabbit antibodies were raised against three different BRD1 epitopes. One (67) was specific for the long BRD1 variant, whereas the two others (63/64 and 65/66) like the original monoclonal mouse antibody (K22) were predicted to stain both variants. Immunohistochemical staining procedures were subsequently performed on paraffin-embedded human cerebral cortex and microarray slides containing 30 different human tissues. Western blotting confirmed the predicted specificity of the developed antibodies. K22, 63/64 and 65/66 displayed a similar neuronal staining pattern characterized by a distinct but weak nuclear staining, while the surrounding cytoplasm and proximal dendrites were more intensely stained. Interestingly, staining with 67 generated in contrast primarily an intense nuclear staining. The new antibodies resulted, furthermore, in a prominent neuroglial reaction characterized by staining of cell bodies, nuclei and glial processes. The tissue microarray analysis revealed that BRD1 was widely distributed in human tissues. The particular expression profile, e.g., the degree of nuclear and/or cytoplasmatic staining, seemed, however, to be highly tissue dependent. These results suggest a general role of BRD1 in the cell and stress that the two BRD1 variants may play different roles in the etiology of psychiatric disease. |
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Abstract We have recently shown that the gene BRD1 is associated with schizophrenia and bipolar affective disorder and that the BRD1 protein (BRD1) which is expressed in neurons may occur in a short and a long variant. The aim of the study was to generate polyclonal antibodies against new BRD1 epitopes enabling discrimination between the long and short BRD1 variants, and elucidate the BRD1 distribution in several human tissues, including the CNS. Polyclonal rabbit antibodies were raised against three different BRD1 epitopes. One (67) was specific for the long BRD1 variant, whereas the two others (63/64 and 65/66) like the original monoclonal mouse antibody (K22) were predicted to stain both variants. Immunohistochemical staining procedures were subsequently performed on paraffin-embedded human cerebral cortex and microarray slides containing 30 different human tissues. Western blotting confirmed the predicted specificity of the developed antibodies. K22, 63/64 and 65/66 displayed a similar neuronal staining pattern characterized by a distinct but weak nuclear staining, while the surrounding cytoplasm and proximal dendrites were more intensely stained. Interestingly, staining with 67 generated in contrast primarily an intense nuclear staining. The new antibodies resulted, furthermore, in a prominent neuroglial reaction characterized by staining of cell bodies, nuclei and glial processes. The tissue microarray analysis revealed that BRD1 was widely distributed in human tissues. The particular expression profile, e.g., the degree of nuclear and/or cytoplasmatic staining, seemed, however, to be highly tissue dependent. These results suggest a general role of BRD1 in the cell and stress that the two BRD1 variants may play different roles in the etiology of psychiatric disease. |
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The aim of the study was to generate polyclonal antibodies against new BRD1 epitopes enabling discrimination between the long and short BRD1 variants, and elucidate the BRD1 distribution in several human tissues, including the CNS. Polyclonal rabbit antibodies were raised against three different BRD1 epitopes. One (67) was specific for the long BRD1 variant, whereas the two others (63/64 and 65/66) like the original monoclonal mouse antibody (K22) were predicted to stain both variants. Immunohistochemical staining procedures were subsequently performed on paraffin-embedded human cerebral cortex and microarray slides containing 30 different human tissues. Western blotting confirmed the predicted specificity of the developed antibodies. K22, 63/64 and 65/66 displayed a similar neuronal staining pattern characterized by a distinct but weak nuclear staining, while the surrounding cytoplasm and proximal dendrites were more intensely stained. Interestingly, staining with 67 generated in contrast primarily an intense nuclear staining. The new antibodies resulted, furthermore, in a prominent neuroglial reaction characterized by staining of cell bodies, nuclei and glial processes. The tissue microarray analysis revealed that BRD1 was widely distributed in human tissues. The particular expression profile, e.g., the degree of nuclear and/or cytoplasmatic staining, seemed, however, to be highly tissue dependent. 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