Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm

Abstract Cholinergic structures in the arm of the cephalopod Octopus vulgaris were studied by immunohistochemistry using specific antisera for two types (common and peripheral) of acetylcholine synthetic enzyme choline acetyltransferase (ChAT): antiserum raised against the rat common type ChAT (cChA...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Sakaue, Yuko [verfasserIn] Bellier, Jean-Pierre [verfasserIn] Kimura, Shin [verfasserIn] D’Este, Loredana [verfasserIn] Takeuchi, Yoshihiro [verfasserIn] Kimura, Hiroshi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	Choline acetyltransferase cChAT pChAT Cephalopod Sensory Motor

Übergeordnetes Werk:	Enthalten in: Anatomy and embryology - Berlin : Springer, 1891, 219(2013), 1 vom: 26. Jan., Seite 323-341
Übergeordnetes Werk:	volume:219 ; year:2013 ; number:1 ; day:26 ; month:01 ; pages:323-341

Links:	Volltext

DOI / URN:	10.1007/s00429-012-0502-6

Katalog-ID:	SPR005720729

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245	1	0	\|a Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm
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520			\|a Abstract Cholinergic structures in the arm of the cephalopod Octopus vulgaris were studied by immunohistochemistry using specific antisera for two types (common and peripheral) of acetylcholine synthetic enzyme choline acetyltransferase (ChAT): antiserum raised against the rat common type ChAT (cChAT), which is cross-reactive with molluscan cChAT, and antiserum raised against the rat peripheral type ChAT (pChAT), which has been used to delineate peripheral cholinergic structures in vertebrates, but not previously in invertebrates. Western blot analysis of octopus extracts revealed a single pChAT-positive band, suggesting that pChAT antiserum is cross-reactive with an octopus counterpart of rat pChAT. In immunohistochemistry, only neuronal structures of the octopus arm were stained by cChAT and pChAT antisera, although the pattern of distribution clearly differed between the two antisera. cChAT-positive varicose nerve fibers were observed in both the cerebrobrachial tract and neuropil of the axial nerve cord, while pChAT-positive varicose fibers were detected only in the neuropil of the axial nerve cord. After epitope retrieval, pChAT-positive neuronal cells and their processes became visible in all ganglia of the arm, including the axial and intramuscular nerve cords, and in ganglia of suckers. Moreover, pChAT-positive structures also became detectable in nerve fibers connecting the different ganglia, in smooth nerve fibers among muscle layers and dermal connective tissues, and in sensory cells of the suckers. These results suggest that the octopus arm has two types of cholinergic nerves: cChAT-positive nerves from brain ganglia and pChAT-positive nerves that are intrinsic to the arm.
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700	1		\|a Kimura, Shin \|e verfasserin \|4 aut
700	1		\|a D’Este, Loredana \|e verfasserin \|4 aut
700	1		\|a Takeuchi, Yoshihiro \|e verfasserin \|4 aut
700	1		\|a Kimura, Hiroshi \|e verfasserin \|4 aut
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allfields	10.1007/s00429-012-0502-6 doi (DE-627)SPR005720729 (SPR)s00429-012-0502-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Sakaue, Yuko verfasserin aut Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Cholinergic structures in the arm of the cephalopod Octopus vulgaris were studied by immunohistochemistry using specific antisera for two types (common and peripheral) of acetylcholine synthetic enzyme choline acetyltransferase (ChAT): antiserum raised against the rat common type ChAT (cChAT), which is cross-reactive with molluscan cChAT, and antiserum raised against the rat peripheral type ChAT (pChAT), which has been used to delineate peripheral cholinergic structures in vertebrates, but not previously in invertebrates. Western blot analysis of octopus extracts revealed a single pChAT-positive band, suggesting that pChAT antiserum is cross-reactive with an octopus counterpart of rat pChAT. In immunohistochemistry, only neuronal structures of the octopus arm were stained by cChAT and pChAT antisera, although the pattern of distribution clearly differed between the two antisera. cChAT-positive varicose nerve fibers were observed in both the cerebrobrachial tract and neuropil of the axial nerve cord, while pChAT-positive varicose fibers were detected only in the neuropil of the axial nerve cord. After epitope retrieval, pChAT-positive neuronal cells and their processes became visible in all ganglia of the arm, including the axial and intramuscular nerve cords, and in ganglia of suckers. Moreover, pChAT-positive structures also became detectable in nerve fibers connecting the different ganglia, in smooth nerve fibers among muscle layers and dermal connective tissues, and in sensory cells of the suckers. These results suggest that the octopus arm has two types of cholinergic nerves: cChAT-positive nerves from brain ganglia and pChAT-positive nerves that are intrinsic to the arm. Choline acetyltransferase (dpeaa)DE-He213 cChAT (dpeaa)DE-He213 pChAT (dpeaa)DE-He213 Cephalopod (dpeaa)DE-He213 Sensory (dpeaa)DE-He213 Motor (dpeaa)DE-He213 Bellier, Jean-Pierre verfasserin aut Kimura, Shin verfasserin aut D’Este, Loredana verfasserin aut Takeuchi, Yoshihiro verfasserin aut Kimura, Hiroshi verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 219(2013), 1 vom: 26. Jan., Seite 323-341 (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:219 year:2013 number:1 day:26 month:01 pages:323-341 https://dx.doi.org/10.1007/s00429-012-0502-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 219 2013 1 26 01 323-341
spelling	10.1007/s00429-012-0502-6 doi (DE-627)SPR005720729 (SPR)s00429-012-0502-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Sakaue, Yuko verfasserin aut Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Cholinergic structures in the arm of the cephalopod Octopus vulgaris were studied by immunohistochemistry using specific antisera for two types (common and peripheral) of acetylcholine synthetic enzyme choline acetyltransferase (ChAT): antiserum raised against the rat common type ChAT (cChAT), which is cross-reactive with molluscan cChAT, and antiserum raised against the rat peripheral type ChAT (pChAT), which has been used to delineate peripheral cholinergic structures in vertebrates, but not previously in invertebrates. Western blot analysis of octopus extracts revealed a single pChAT-positive band, suggesting that pChAT antiserum is cross-reactive with an octopus counterpart of rat pChAT. In immunohistochemistry, only neuronal structures of the octopus arm were stained by cChAT and pChAT antisera, although the pattern of distribution clearly differed between the two antisera. cChAT-positive varicose nerve fibers were observed in both the cerebrobrachial tract and neuropil of the axial nerve cord, while pChAT-positive varicose fibers were detected only in the neuropil of the axial nerve cord. After epitope retrieval, pChAT-positive neuronal cells and their processes became visible in all ganglia of the arm, including the axial and intramuscular nerve cords, and in ganglia of suckers. Moreover, pChAT-positive structures also became detectable in nerve fibers connecting the different ganglia, in smooth nerve fibers among muscle layers and dermal connective tissues, and in sensory cells of the suckers. These results suggest that the octopus arm has two types of cholinergic nerves: cChAT-positive nerves from brain ganglia and pChAT-positive nerves that are intrinsic to the arm. Choline acetyltransferase (dpeaa)DE-He213 cChAT (dpeaa)DE-He213 pChAT (dpeaa)DE-He213 Cephalopod (dpeaa)DE-He213 Sensory (dpeaa)DE-He213 Motor (dpeaa)DE-He213 Bellier, Jean-Pierre verfasserin aut Kimura, Shin verfasserin aut D’Este, Loredana verfasserin aut Takeuchi, Yoshihiro verfasserin aut Kimura, Hiroshi verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 219(2013), 1 vom: 26. Jan., Seite 323-341 (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:219 year:2013 number:1 day:26 month:01 pages:323-341 https://dx.doi.org/10.1007/s00429-012-0502-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 219 2013 1 26 01 323-341
allfields_unstemmed	10.1007/s00429-012-0502-6 doi (DE-627)SPR005720729 (SPR)s00429-012-0502-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Sakaue, Yuko verfasserin aut Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Cholinergic structures in the arm of the cephalopod Octopus vulgaris were studied by immunohistochemistry using specific antisera for two types (common and peripheral) of acetylcholine synthetic enzyme choline acetyltransferase (ChAT): antiserum raised against the rat common type ChAT (cChAT), which is cross-reactive with molluscan cChAT, and antiserum raised against the rat peripheral type ChAT (pChAT), which has been used to delineate peripheral cholinergic structures in vertebrates, but not previously in invertebrates. Western blot analysis of octopus extracts revealed a single pChAT-positive band, suggesting that pChAT antiserum is cross-reactive with an octopus counterpart of rat pChAT. In immunohistochemistry, only neuronal structures of the octopus arm were stained by cChAT and pChAT antisera, although the pattern of distribution clearly differed between the two antisera. cChAT-positive varicose nerve fibers were observed in both the cerebrobrachial tract and neuropil of the axial nerve cord, while pChAT-positive varicose fibers were detected only in the neuropil of the axial nerve cord. After epitope retrieval, pChAT-positive neuronal cells and their processes became visible in all ganglia of the arm, including the axial and intramuscular nerve cords, and in ganglia of suckers. Moreover, pChAT-positive structures also became detectable in nerve fibers connecting the different ganglia, in smooth nerve fibers among muscle layers and dermal connective tissues, and in sensory cells of the suckers. These results suggest that the octopus arm has two types of cholinergic nerves: cChAT-positive nerves from brain ganglia and pChAT-positive nerves that are intrinsic to the arm. Choline acetyltransferase (dpeaa)DE-He213 cChAT (dpeaa)DE-He213 pChAT (dpeaa)DE-He213 Cephalopod (dpeaa)DE-He213 Sensory (dpeaa)DE-He213 Motor (dpeaa)DE-He213 Bellier, Jean-Pierre verfasserin aut Kimura, Shin verfasserin aut D’Este, Loredana verfasserin aut Takeuchi, Yoshihiro verfasserin aut Kimura, Hiroshi verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 219(2013), 1 vom: 26. Jan., Seite 323-341 (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:219 year:2013 number:1 day:26 month:01 pages:323-341 https://dx.doi.org/10.1007/s00429-012-0502-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 219 2013 1 26 01 323-341
allfieldsGer	10.1007/s00429-012-0502-6 doi (DE-627)SPR005720729 (SPR)s00429-012-0502-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Sakaue, Yuko verfasserin aut Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Cholinergic structures in the arm of the cephalopod Octopus vulgaris were studied by immunohistochemistry using specific antisera for two types (common and peripheral) of acetylcholine synthetic enzyme choline acetyltransferase (ChAT): antiserum raised against the rat common type ChAT (cChAT), which is cross-reactive with molluscan cChAT, and antiserum raised against the rat peripheral type ChAT (pChAT), which has been used to delineate peripheral cholinergic structures in vertebrates, but not previously in invertebrates. Western blot analysis of octopus extracts revealed a single pChAT-positive band, suggesting that pChAT antiserum is cross-reactive with an octopus counterpart of rat pChAT. In immunohistochemistry, only neuronal structures of the octopus arm were stained by cChAT and pChAT antisera, although the pattern of distribution clearly differed between the two antisera. cChAT-positive varicose nerve fibers were observed in both the cerebrobrachial tract and neuropil of the axial nerve cord, while pChAT-positive varicose fibers were detected only in the neuropil of the axial nerve cord. After epitope retrieval, pChAT-positive neuronal cells and their processes became visible in all ganglia of the arm, including the axial and intramuscular nerve cords, and in ganglia of suckers. Moreover, pChAT-positive structures also became detectable in nerve fibers connecting the different ganglia, in smooth nerve fibers among muscle layers and dermal connective tissues, and in sensory cells of the suckers. These results suggest that the octopus arm has two types of cholinergic nerves: cChAT-positive nerves from brain ganglia and pChAT-positive nerves that are intrinsic to the arm. Choline acetyltransferase (dpeaa)DE-He213 cChAT (dpeaa)DE-He213 pChAT (dpeaa)DE-He213 Cephalopod (dpeaa)DE-He213 Sensory (dpeaa)DE-He213 Motor (dpeaa)DE-He213 Bellier, Jean-Pierre verfasserin aut Kimura, Shin verfasserin aut D’Este, Loredana verfasserin aut Takeuchi, Yoshihiro verfasserin aut Kimura, Hiroshi verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 219(2013), 1 vom: 26. Jan., Seite 323-341 (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:219 year:2013 number:1 day:26 month:01 pages:323-341 https://dx.doi.org/10.1007/s00429-012-0502-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 219 2013 1 26 01 323-341
allfieldsSound	10.1007/s00429-012-0502-6 doi (DE-627)SPR005720729 (SPR)s00429-012-0502-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Sakaue, Yuko verfasserin aut Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Cholinergic structures in the arm of the cephalopod Octopus vulgaris were studied by immunohistochemistry using specific antisera for two types (common and peripheral) of acetylcholine synthetic enzyme choline acetyltransferase (ChAT): antiserum raised against the rat common type ChAT (cChAT), which is cross-reactive with molluscan cChAT, and antiserum raised against the rat peripheral type ChAT (pChAT), which has been used to delineate peripheral cholinergic structures in vertebrates, but not previously in invertebrates. Western blot analysis of octopus extracts revealed a single pChAT-positive band, suggesting that pChAT antiserum is cross-reactive with an octopus counterpart of rat pChAT. In immunohistochemistry, only neuronal structures of the octopus arm were stained by cChAT and pChAT antisera, although the pattern of distribution clearly differed between the two antisera. cChAT-positive varicose nerve fibers were observed in both the cerebrobrachial tract and neuropil of the axial nerve cord, while pChAT-positive varicose fibers were detected only in the neuropil of the axial nerve cord. After epitope retrieval, pChAT-positive neuronal cells and their processes became visible in all ganglia of the arm, including the axial and intramuscular nerve cords, and in ganglia of suckers. Moreover, pChAT-positive structures also became detectable in nerve fibers connecting the different ganglia, in smooth nerve fibers among muscle layers and dermal connective tissues, and in sensory cells of the suckers. These results suggest that the octopus arm has two types of cholinergic nerves: cChAT-positive nerves from brain ganglia and pChAT-positive nerves that are intrinsic to the arm. Choline acetyltransferase (dpeaa)DE-He213 cChAT (dpeaa)DE-He213 pChAT (dpeaa)DE-He213 Cephalopod (dpeaa)DE-He213 Sensory (dpeaa)DE-He213 Motor (dpeaa)DE-He213 Bellier, Jean-Pierre verfasserin aut Kimura, Shin verfasserin aut D’Este, Loredana verfasserin aut Takeuchi, Yoshihiro verfasserin aut Kimura, Hiroshi verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 219(2013), 1 vom: 26. Jan., Seite 323-341 (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:219 year:2013 number:1 day:26 month:01 pages:323-341 https://dx.doi.org/10.1007/s00429-012-0502-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 219 2013 1 26 01 323-341
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source	Enthalten in Anatomy and embryology 219(2013), 1 vom: 26. Jan., Seite 323-341 volume:219 year:2013 number:1 day:26 month:01 pages:323-341
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authorswithroles_txt_mv	Sakaue, Yuko @@aut@@ Bellier, Jean-Pierre @@aut@@ Kimura, Shin @@aut@@ D’Este, Loredana @@aut@@ Takeuchi, Yoshihiro @@aut@@ Kimura, Hiroshi @@aut@@
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author	Sakaue, Yuko
spellingShingle	Sakaue, Yuko ddc 610 bkl 44.34 misc Choline acetyltransferase misc cChAT misc pChAT misc Cephalopod misc Sensory misc Motor Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm
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topic_title	610 ASE 44.34 bkl Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm Choline acetyltransferase (dpeaa)DE-He213 cChAT (dpeaa)DE-He213 pChAT (dpeaa)DE-He213 Cephalopod (dpeaa)DE-He213 Sensory (dpeaa)DE-He213 Motor (dpeaa)DE-He213
topic	ddc 610 bkl 44.34 misc Choline acetyltransferase misc cChAT misc pChAT misc Cephalopod misc Sensory misc Motor
topic_unstemmed	ddc 610 bkl 44.34 misc Choline acetyltransferase misc cChAT misc pChAT misc Cephalopod misc Sensory misc Motor
topic_browse	ddc 610 bkl 44.34 misc Choline acetyltransferase misc cChAT misc pChAT misc Cephalopod misc Sensory misc Motor
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title	Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm
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title_full	Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm
author_sort	Sakaue, Yuko
journal	Anatomy and embryology
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author_browse	Sakaue, Yuko Bellier, Jean-Pierre Kimura, Shin D’Este, Loredana Takeuchi, Yoshihiro Kimura, Hiroshi
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title_sort	immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm
title_auth	Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm
abstract	Abstract Cholinergic structures in the arm of the cephalopod Octopus vulgaris were studied by immunohistochemistry using specific antisera for two types (common and peripheral) of acetylcholine synthetic enzyme choline acetyltransferase (ChAT): antiserum raised against the rat common type ChAT (cChAT), which is cross-reactive with molluscan cChAT, and antiserum raised against the rat peripheral type ChAT (pChAT), which has been used to delineate peripheral cholinergic structures in vertebrates, but not previously in invertebrates. Western blot analysis of octopus extracts revealed a single pChAT-positive band, suggesting that pChAT antiserum is cross-reactive with an octopus counterpart of rat pChAT. In immunohistochemistry, only neuronal structures of the octopus arm were stained by cChAT and pChAT antisera, although the pattern of distribution clearly differed between the two antisera. cChAT-positive varicose nerve fibers were observed in both the cerebrobrachial tract and neuropil of the axial nerve cord, while pChAT-positive varicose fibers were detected only in the neuropil of the axial nerve cord. After epitope retrieval, pChAT-positive neuronal cells and their processes became visible in all ganglia of the arm, including the axial and intramuscular nerve cords, and in ganglia of suckers. Moreover, pChAT-positive structures also became detectable in nerve fibers connecting the different ganglia, in smooth nerve fibers among muscle layers and dermal connective tissues, and in sensory cells of the suckers. These results suggest that the octopus arm has two types of cholinergic nerves: cChAT-positive nerves from brain ganglia and pChAT-positive nerves that are intrinsic to the arm.
abstractGer	Abstract Cholinergic structures in the arm of the cephalopod Octopus vulgaris were studied by immunohistochemistry using specific antisera for two types (common and peripheral) of acetylcholine synthetic enzyme choline acetyltransferase (ChAT): antiserum raised against the rat common type ChAT (cChAT), which is cross-reactive with molluscan cChAT, and antiserum raised against the rat peripheral type ChAT (pChAT), which has been used to delineate peripheral cholinergic structures in vertebrates, but not previously in invertebrates. Western blot analysis of octopus extracts revealed a single pChAT-positive band, suggesting that pChAT antiserum is cross-reactive with an octopus counterpart of rat pChAT. In immunohistochemistry, only neuronal structures of the octopus arm were stained by cChAT and pChAT antisera, although the pattern of distribution clearly differed between the two antisera. cChAT-positive varicose nerve fibers were observed in both the cerebrobrachial tract and neuropil of the axial nerve cord, while pChAT-positive varicose fibers were detected only in the neuropil of the axial nerve cord. After epitope retrieval, pChAT-positive neuronal cells and their processes became visible in all ganglia of the arm, including the axial and intramuscular nerve cords, and in ganglia of suckers. Moreover, pChAT-positive structures also became detectable in nerve fibers connecting the different ganglia, in smooth nerve fibers among muscle layers and dermal connective tissues, and in sensory cells of the suckers. These results suggest that the octopus arm has two types of cholinergic nerves: cChAT-positive nerves from brain ganglia and pChAT-positive nerves that are intrinsic to the arm.
abstract_unstemmed	Abstract Cholinergic structures in the arm of the cephalopod Octopus vulgaris were studied by immunohistochemistry using specific antisera for two types (common and peripheral) of acetylcholine synthetic enzyme choline acetyltransferase (ChAT): antiserum raised against the rat common type ChAT (cChAT), which is cross-reactive with molluscan cChAT, and antiserum raised against the rat peripheral type ChAT (pChAT), which has been used to delineate peripheral cholinergic structures in vertebrates, but not previously in invertebrates. Western blot analysis of octopus extracts revealed a single pChAT-positive band, suggesting that pChAT antiserum is cross-reactive with an octopus counterpart of rat pChAT. In immunohistochemistry, only neuronal structures of the octopus arm were stained by cChAT and pChAT antisera, although the pattern of distribution clearly differed between the two antisera. cChAT-positive varicose nerve fibers were observed in both the cerebrobrachial tract and neuropil of the axial nerve cord, while pChAT-positive varicose fibers were detected only in the neuropil of the axial nerve cord. After epitope retrieval, pChAT-positive neuronal cells and their processes became visible in all ganglia of the arm, including the axial and intramuscular nerve cords, and in ganglia of suckers. Moreover, pChAT-positive structures also became detectable in nerve fibers connecting the different ganglia, in smooth nerve fibers among muscle layers and dermal connective tissues, and in sensory cells of the suckers. These results suggest that the octopus arm has two types of cholinergic nerves: cChAT-positive nerves from brain ganglia and pChAT-positive nerves that are intrinsic to the arm.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711
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title_short	Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm
url	https://dx.doi.org/10.1007/s00429-012-0502-6
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author2	Bellier, Jean-Pierre Kimura, Shin D’Este, Loredana Takeuchi, Yoshihiro Kimura, Hiroshi
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up_date	2024-07-03T18:15:06.068Z
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Western blot analysis of octopus extracts revealed a single pChAT-positive band, suggesting that pChAT antiserum is cross-reactive with an octopus counterpart of rat pChAT. In immunohistochemistry, only neuronal structures of the octopus arm were stained by cChAT and pChAT antisera, although the pattern of distribution clearly differed between the two antisera. cChAT-positive varicose nerve fibers were observed in both the cerebrobrachial tract and neuropil of the axial nerve cord, while pChAT-positive varicose fibers were detected only in the neuropil of the axial nerve cord. After epitope retrieval, pChAT-positive neuronal cells and their processes became visible in all ganglia of the arm, including the axial and intramuscular nerve cords, and in ganglia of suckers. Moreover, pChAT-positive structures also became detectable in nerve fibers connecting the different ganglia, in smooth nerve fibers among muscle layers and dermal connective tissues, and in sensory cells of the suckers. 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Immunohistochemical localization of two types of choline acetyltransferase in neurons and sensory cells of the octopus arm

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