Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism
Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demo...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Sierra, Salvador [verfasserIn] Luquin, Natasha [verfasserIn] Rico, Alberto J. [verfasserIn] Gómez-Bautista, Virginia [verfasserIn] Roda, Elvira [verfasserIn] Dopeso-Reyes, Iria G. [verfasserIn] Vázquez, Alfonso [verfasserIn] Martínez-Pinilla, Eva [verfasserIn] Labandeira-García, José L. [verfasserIn] Franco, Rafael [verfasserIn] Lanciego, José L. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Anatomy and embryology - Berlin : Springer, 1891, 220(2014), 5 vom: 28. Juni, Seite 2721-2738 |
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| Übergeordnetes Werk: |
volume:220 ; year:2014 ; number:5 ; day:28 ; month:06 ; pages:2721-2738 |
| Links: |
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| DOI / URN: |
10.1007/s00429-014-0823-8 |
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SPR005724244 |
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| 245 | 1 | 0 | |a Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism |
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| 520 | |a Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of $ CB_{1} $Rs and $ CB_{2} $Rs, as well as $ CB_{1} $R–$ CB_{2} $R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of $ CB_{1} $Rs, $ CB_{2} $Rs and $ CB_{1} $R–$ CB_{2} $R heteromers was found in dyskinetic animals, mimicking the observed reduction in $ CB_{1} $R and $ CB_{2} $R mRNA expression levels. The fact that chronic levodopa treatment disrupted $ CB_{1} $R–$ CB_{2} $R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers. | ||
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| 700 | 1 | |a Luquin, Natasha |e verfasserin |4 aut | |
| 700 | 1 | |a Rico, Alberto J. |e verfasserin |4 aut | |
| 700 | 1 | |a Gómez-Bautista, Virginia |e verfasserin |4 aut | |
| 700 | 1 | |a Roda, Elvira |e verfasserin |4 aut | |
| 700 | 1 | |a Dopeso-Reyes, Iria G. |e verfasserin |4 aut | |
| 700 | 1 | |a Vázquez, Alfonso |e verfasserin |4 aut | |
| 700 | 1 | |a Martínez-Pinilla, Eva |e verfasserin |4 aut | |
| 700 | 1 | |a Labandeira-García, José L. |e verfasserin |4 aut | |
| 700 | 1 | |a Franco, Rafael |e verfasserin |4 aut | |
| 700 | 1 | |a Lanciego, José L. |e verfasserin |4 aut | |
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10.1007/s00429-014-0823-8 doi (DE-627)SPR005724244 (SPR)s00429-014-0823-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Sierra, Salvador verfasserin aut Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of $ CB_{1} $Rs and $ CB_{2} $Rs, as well as $ CB_{1} $R–$ CB_{2} $R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of $ CB_{1} $Rs, $ CB_{2} $Rs and $ CB_{1} $R–$ CB_{2} $R heteromers was found in dyskinetic animals, mimicking the observed reduction in $ CB_{1} $R and $ CB_{2} $R mRNA expression levels. The fact that chronic levodopa treatment disrupted $ CB_{1} $R–$ CB_{2} $R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers. GPCRs (dpeaa)DE-He213 Cannabis (dpeaa)DE-He213 Cannabinoid receptor heteromer (dpeaa)DE-He213 Dyskinesia (dpeaa)DE-He213 Globus pallidus (dpeaa)DE-He213 MPTP (dpeaa)DE-He213 Luquin, Natasha verfasserin aut Rico, Alberto J. verfasserin aut Gómez-Bautista, Virginia verfasserin aut Roda, Elvira verfasserin aut Dopeso-Reyes, Iria G. verfasserin aut Vázquez, Alfonso verfasserin aut Martínez-Pinilla, Eva verfasserin aut Labandeira-García, José L. verfasserin aut Franco, Rafael verfasserin aut Lanciego, José L. verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 220(2014), 5 vom: 28. Juni, Seite 2721-2738 (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:220 year:2014 number:5 day:28 month:06 pages:2721-2738 https://dx.doi.org/10.1007/s00429-014-0823-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 220 2014 5 28 06 2721-2738 |
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10.1007/s00429-014-0823-8 doi (DE-627)SPR005724244 (SPR)s00429-014-0823-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Sierra, Salvador verfasserin aut Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of $ CB_{1} $Rs and $ CB_{2} $Rs, as well as $ CB_{1} $R–$ CB_{2} $R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of $ CB_{1} $Rs, $ CB_{2} $Rs and $ CB_{1} $R–$ CB_{2} $R heteromers was found in dyskinetic animals, mimicking the observed reduction in $ CB_{1} $R and $ CB_{2} $R mRNA expression levels. The fact that chronic levodopa treatment disrupted $ CB_{1} $R–$ CB_{2} $R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers. GPCRs (dpeaa)DE-He213 Cannabis (dpeaa)DE-He213 Cannabinoid receptor heteromer (dpeaa)DE-He213 Dyskinesia (dpeaa)DE-He213 Globus pallidus (dpeaa)DE-He213 MPTP (dpeaa)DE-He213 Luquin, Natasha verfasserin aut Rico, Alberto J. verfasserin aut Gómez-Bautista, Virginia verfasserin aut Roda, Elvira verfasserin aut Dopeso-Reyes, Iria G. verfasserin aut Vázquez, Alfonso verfasserin aut Martínez-Pinilla, Eva verfasserin aut Labandeira-García, José L. verfasserin aut Franco, Rafael verfasserin aut Lanciego, José L. verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 220(2014), 5 vom: 28. Juni, Seite 2721-2738 (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:220 year:2014 number:5 day:28 month:06 pages:2721-2738 https://dx.doi.org/10.1007/s00429-014-0823-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 220 2014 5 28 06 2721-2738 |
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10.1007/s00429-014-0823-8 doi (DE-627)SPR005724244 (SPR)s00429-014-0823-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Sierra, Salvador verfasserin aut Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of $ CB_{1} $Rs and $ CB_{2} $Rs, as well as $ CB_{1} $R–$ CB_{2} $R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of $ CB_{1} $Rs, $ CB_{2} $Rs and $ CB_{1} $R–$ CB_{2} $R heteromers was found in dyskinetic animals, mimicking the observed reduction in $ CB_{1} $R and $ CB_{2} $R mRNA expression levels. The fact that chronic levodopa treatment disrupted $ CB_{1} $R–$ CB_{2} $R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers. GPCRs (dpeaa)DE-He213 Cannabis (dpeaa)DE-He213 Cannabinoid receptor heteromer (dpeaa)DE-He213 Dyskinesia (dpeaa)DE-He213 Globus pallidus (dpeaa)DE-He213 MPTP (dpeaa)DE-He213 Luquin, Natasha verfasserin aut Rico, Alberto J. verfasserin aut Gómez-Bautista, Virginia verfasserin aut Roda, Elvira verfasserin aut Dopeso-Reyes, Iria G. verfasserin aut Vázquez, Alfonso verfasserin aut Martínez-Pinilla, Eva verfasserin aut Labandeira-García, José L. verfasserin aut Franco, Rafael verfasserin aut Lanciego, José L. verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 220(2014), 5 vom: 28. Juni, Seite 2721-2738 (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:220 year:2014 number:5 day:28 month:06 pages:2721-2738 https://dx.doi.org/10.1007/s00429-014-0823-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 220 2014 5 28 06 2721-2738 |
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10.1007/s00429-014-0823-8 doi (DE-627)SPR005724244 (SPR)s00429-014-0823-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Sierra, Salvador verfasserin aut Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of $ CB_{1} $Rs and $ CB_{2} $Rs, as well as $ CB_{1} $R–$ CB_{2} $R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of $ CB_{1} $Rs, $ CB_{2} $Rs and $ CB_{1} $R–$ CB_{2} $R heteromers was found in dyskinetic animals, mimicking the observed reduction in $ CB_{1} $R and $ CB_{2} $R mRNA expression levels. The fact that chronic levodopa treatment disrupted $ CB_{1} $R–$ CB_{2} $R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers. GPCRs (dpeaa)DE-He213 Cannabis (dpeaa)DE-He213 Cannabinoid receptor heteromer (dpeaa)DE-He213 Dyskinesia (dpeaa)DE-He213 Globus pallidus (dpeaa)DE-He213 MPTP (dpeaa)DE-He213 Luquin, Natasha verfasserin aut Rico, Alberto J. verfasserin aut Gómez-Bautista, Virginia verfasserin aut Roda, Elvira verfasserin aut Dopeso-Reyes, Iria G. verfasserin aut Vázquez, Alfonso verfasserin aut Martínez-Pinilla, Eva verfasserin aut Labandeira-García, José L. verfasserin aut Franco, Rafael verfasserin aut Lanciego, José L. verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 220(2014), 5 vom: 28. Juni, Seite 2721-2738 (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:220 year:2014 number:5 day:28 month:06 pages:2721-2738 https://dx.doi.org/10.1007/s00429-014-0823-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 220 2014 5 28 06 2721-2738 |
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10.1007/s00429-014-0823-8 doi (DE-627)SPR005724244 (SPR)s00429-014-0823-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl Sierra, Salvador verfasserin aut Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of $ CB_{1} $Rs and $ CB_{2} $Rs, as well as $ CB_{1} $R–$ CB_{2} $R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of $ CB_{1} $Rs, $ CB_{2} $Rs and $ CB_{1} $R–$ CB_{2} $R heteromers was found in dyskinetic animals, mimicking the observed reduction in $ CB_{1} $R and $ CB_{2} $R mRNA expression levels. The fact that chronic levodopa treatment disrupted $ CB_{1} $R–$ CB_{2} $R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers. GPCRs (dpeaa)DE-He213 Cannabis (dpeaa)DE-He213 Cannabinoid receptor heteromer (dpeaa)DE-He213 Dyskinesia (dpeaa)DE-He213 Globus pallidus (dpeaa)DE-He213 MPTP (dpeaa)DE-He213 Luquin, Natasha verfasserin aut Rico, Alberto J. verfasserin aut Gómez-Bautista, Virginia verfasserin aut Roda, Elvira verfasserin aut Dopeso-Reyes, Iria G. verfasserin aut Vázquez, Alfonso verfasserin aut Martínez-Pinilla, Eva verfasserin aut Labandeira-García, José L. verfasserin aut Franco, Rafael verfasserin aut Lanciego, José L. verfasserin aut Enthalten in Anatomy and embryology Berlin : Springer, 1891 220(2014), 5 vom: 28. Juni, Seite 2721-2738 (DE-627)253389798 (DE-600)1458423-2 1432-0568 nnns volume:220 year:2014 number:5 day:28 month:06 pages:2721-2738 https://dx.doi.org/10.1007/s00429-014-0823-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_711 44.34 ASE AR 220 2014 5 28 06 2721-2738 |
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Enthalten in Anatomy and embryology 220(2014), 5 vom: 28. Juni, Seite 2721-2738 volume:220 year:2014 number:5 day:28 month:06 pages:2721-2738 |
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Sierra, Salvador ddc 610 bkl 44.34 misc GPCRs misc Cannabis misc Cannabinoid receptor heteromer misc Dyskinesia misc Globus pallidus misc MPTP Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism |
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610 ASE 44.34 bkl Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism GPCRs (dpeaa)DE-He213 Cannabis (dpeaa)DE-He213 Cannabinoid receptor heteromer (dpeaa)DE-He213 Dyskinesia (dpeaa)DE-He213 Globus pallidus (dpeaa)DE-He213 MPTP (dpeaa)DE-He213 |
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Sierra, Salvador Luquin, Natasha Rico, Alberto J. Gómez-Bautista, Virginia Roda, Elvira Dopeso-Reyes, Iria G. Vázquez, Alfonso Martínez-Pinilla, Eva Labandeira-García, José L. Franco, Rafael Lanciego, José L. |
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detection of cannabinoid receptors cb1 and cb2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism |
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Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism |
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Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of $ CB_{1} $Rs and $ CB_{2} $Rs, as well as $ CB_{1} $R–$ CB_{2} $R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of $ CB_{1} $Rs, $ CB_{2} $Rs and $ CB_{1} $R–$ CB_{2} $R heteromers was found in dyskinetic animals, mimicking the observed reduction in $ CB_{1} $R and $ CB_{2} $R mRNA expression levels. The fact that chronic levodopa treatment disrupted $ CB_{1} $R–$ CB_{2} $R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers. |
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Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of $ CB_{1} $Rs and $ CB_{2} $Rs, as well as $ CB_{1} $R–$ CB_{2} $R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of $ CB_{1} $Rs, $ CB_{2} $Rs and $ CB_{1} $R–$ CB_{2} $R heteromers was found in dyskinetic animals, mimicking the observed reduction in $ CB_{1} $R and $ CB_{2} $R mRNA expression levels. The fact that chronic levodopa treatment disrupted $ CB_{1} $R–$ CB_{2} $R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers. |
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Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of $ CB_{1} $Rs and $ CB_{2} $Rs, as well as $ CB_{1} $R–$ CB_{2} $R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of $ CB_{1} $Rs, $ CB_{2} $Rs and $ CB_{1} $R–$ CB_{2} $R heteromers was found in dyskinetic animals, mimicking the observed reduction in $ CB_{1} $R and $ CB_{2} $R mRNA expression levels. The fact that chronic levodopa treatment disrupted $ CB_{1} $R–$ CB_{2} $R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers. |
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Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR005724244</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519220444.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201002s2014 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00429-014-0823-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR005724244</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00429-014-0823-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.34</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Sierra, Salvador</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2014</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Although type 1 cannabinoid receptors ($ CB_{1} $Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors ($ CB_{2} $Rs) in neurons is still somewhat controversial. Taking advantage of newly designed $ CB_{1} $R and $ CB_{2} $R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of $ CB_{1} $Rs and $ CB_{2} $Rs, as well as $ CB_{1} $R–$ CB_{2} $R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of $ CB_{1} $Rs, $ CB_{2} $Rs and $ CB_{1} $R–$ CB_{2} $R heteromers was found in dyskinetic animals, mimicking the observed reduction in $ CB_{1} $R and $ CB_{2} $R mRNA expression levels. 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