An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa

Abstract. Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Mäser, Pascal [verfasserIn] Grether-Bühler, Yvonne [verfasserIn] Kaminsky, Ronald [verfasserIn] Brun, Reto [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2002

Übergeordnetes Werk:	Enthalten in: Parasitology research - Berlin : Springer, 1928, 88(2002), 2 vom: Feb., Seite 172-174
Übergeordnetes Werk:	volume:88 ; year:2002 ; number:2 ; month:02 ; pages:172-174

Links:	Volltext

DOI / URN:	10.1007/s00436-001-0511-5

Katalog-ID:	SPR005885051

Internformat


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024	7		\|a 10.1007/s00436-001-0511-5 \|2 doi
035			\|a (DE-627)SPR005885051
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040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
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082	0	4	\|a 590 \|a 610 \|q ASE
084			\|a 44.44 \|2 bkl
084			\|a 42.00 \|2 bkl
100	1		\|a Mäser, Pascal \|e verfasserin \|4 aut
245	1	3	\|a An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa
264		1	\|c 2002
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
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520			\|a Abstract. Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G (60 $ µgml^{–1} $), kanamycin (100 $ µgml^{–1} $), chloramphenicol (10 $ µgml^{–1} $) and flucytosine (50 $ µgml^{–1} $). It is well tolerated by African and South American trypanosomes, Leishmania spp., Giardia duodenalis, Entamoeba histolytica and Plasmodium falciparum, and it provides an effective means for the treatment of contaminated cultures. The anti-contamination cocktail meets numerous prophylactic applications in which sterile handling is impracticable or impossible. In particular, it facilitates the direct in vitro isolation of parasites from an infected host.
700	1		\|a Grether-Bühler, Yvonne \|e verfasserin \|4 aut
700	1		\|a Kaminsky, Ronald \|e verfasserin \|4 aut
700	1		\|a Brun, Reto \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Parasitology research \|d Berlin : Springer, 1928 \|g 88(2002), 2 vom: Feb., Seite 172-174 \|w (DE-627)254638627 \|w (DE-600)1462976-8 \|x 1432-1955 \|7 nnns
773	1	8	\|g volume:88 \|g year:2002 \|g number:2 \|g month:02 \|g pages:172-174
856	4	0	\|u https://dx.doi.org/10.1007/s00436-001-0511-5 \|z lizenzpflichtig \|3 Volltext
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_SPRINGER
912			\|a SSG-OLC-PHA
912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_121
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_267
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_374
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_647
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2018
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2043
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2158
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2193
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_2808
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4277
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4328
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4346
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
912			\|a GBV_ILN_4753
936	b	k	\|a 44.44 \|q ASE
936	b	k	\|a 42.00 \|q ASE
951			\|a AR
952			\|d 88 \|j 2002 \|e 2 \|c 02 \|h 172-174

Indexfelder

author_variant	p m pm y g b ygb r k rk r b rb
matchkey_str	article:14321955:2002----::nniotmntocctifrhivtosltoadutvt
hierarchy_sort_str	2002
bklnumber	44.44 42.00
publishDate	2002
allfields	10.1007/s00436-001-0511-5 doi (DE-627)SPR005885051 (SPR)s00436-001-0511-5-e DE-627 ger DE-627 rakwb eng 590 610 ASE 44.44 bkl 42.00 bkl Mäser, Pascal verfasserin aut An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract. Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G (60 $ µgml^{–1} $), kanamycin (100 $ µgml^{–1} $), chloramphenicol (10 $ µgml^{–1} $) and flucytosine (50 $ µgml^{–1} $). It is well tolerated by African and South American trypanosomes, Leishmania spp., Giardia duodenalis, Entamoeba histolytica and Plasmodium falciparum, and it provides an effective means for the treatment of contaminated cultures. The anti-contamination cocktail meets numerous prophylactic applications in which sterile handling is impracticable or impossible. In particular, it facilitates the direct in vitro isolation of parasites from an infected host. Grether-Bühler, Yvonne verfasserin aut Kaminsky, Ronald verfasserin aut Brun, Reto verfasserin aut Enthalten in Parasitology research Berlin : Springer, 1928 88(2002), 2 vom: Feb., Seite 172-174 (DE-627)254638627 (DE-600)1462976-8 1432-1955 nnns volume:88 year:2002 number:2 month:02 pages:172-174 https://dx.doi.org/10.1007/s00436-001-0511-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2043 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2158 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2193 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2808 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 44.44 ASE 42.00 ASE AR 88 2002 2 02 172-174
spelling	10.1007/s00436-001-0511-5 doi (DE-627)SPR005885051 (SPR)s00436-001-0511-5-e DE-627 ger DE-627 rakwb eng 590 610 ASE 44.44 bkl 42.00 bkl Mäser, Pascal verfasserin aut An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract. Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G (60 $ µgml^{–1} $), kanamycin (100 $ µgml^{–1} $), chloramphenicol (10 $ µgml^{–1} $) and flucytosine (50 $ µgml^{–1} $). It is well tolerated by African and South American trypanosomes, Leishmania spp., Giardia duodenalis, Entamoeba histolytica and Plasmodium falciparum, and it provides an effective means for the treatment of contaminated cultures. The anti-contamination cocktail meets numerous prophylactic applications in which sterile handling is impracticable or impossible. In particular, it facilitates the direct in vitro isolation of parasites from an infected host. Grether-Bühler, Yvonne verfasserin aut Kaminsky, Ronald verfasserin aut Brun, Reto verfasserin aut Enthalten in Parasitology research Berlin : Springer, 1928 88(2002), 2 vom: Feb., Seite 172-174 (DE-627)254638627 (DE-600)1462976-8 1432-1955 nnns volume:88 year:2002 number:2 month:02 pages:172-174 https://dx.doi.org/10.1007/s00436-001-0511-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2043 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2158 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2193 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2808 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 44.44 ASE 42.00 ASE AR 88 2002 2 02 172-174
allfields_unstemmed	10.1007/s00436-001-0511-5 doi (DE-627)SPR005885051 (SPR)s00436-001-0511-5-e DE-627 ger DE-627 rakwb eng 590 610 ASE 44.44 bkl 42.00 bkl Mäser, Pascal verfasserin aut An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract. Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G (60 $ µgml^{–1} $), kanamycin (100 $ µgml^{–1} $), chloramphenicol (10 $ µgml^{–1} $) and flucytosine (50 $ µgml^{–1} $). It is well tolerated by African and South American trypanosomes, Leishmania spp., Giardia duodenalis, Entamoeba histolytica and Plasmodium falciparum, and it provides an effective means for the treatment of contaminated cultures. The anti-contamination cocktail meets numerous prophylactic applications in which sterile handling is impracticable or impossible. In particular, it facilitates the direct in vitro isolation of parasites from an infected host. Grether-Bühler, Yvonne verfasserin aut Kaminsky, Ronald verfasserin aut Brun, Reto verfasserin aut Enthalten in Parasitology research Berlin : Springer, 1928 88(2002), 2 vom: Feb., Seite 172-174 (DE-627)254638627 (DE-600)1462976-8 1432-1955 nnns volume:88 year:2002 number:2 month:02 pages:172-174 https://dx.doi.org/10.1007/s00436-001-0511-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2043 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2158 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2193 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2808 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 44.44 ASE 42.00 ASE AR 88 2002 2 02 172-174
allfieldsGer	10.1007/s00436-001-0511-5 doi (DE-627)SPR005885051 (SPR)s00436-001-0511-5-e DE-627 ger DE-627 rakwb eng 590 610 ASE 44.44 bkl 42.00 bkl Mäser, Pascal verfasserin aut An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract. Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G (60 $ µgml^{–1} $), kanamycin (100 $ µgml^{–1} $), chloramphenicol (10 $ µgml^{–1} $) and flucytosine (50 $ µgml^{–1} $). It is well tolerated by African and South American trypanosomes, Leishmania spp., Giardia duodenalis, Entamoeba histolytica and Plasmodium falciparum, and it provides an effective means for the treatment of contaminated cultures. The anti-contamination cocktail meets numerous prophylactic applications in which sterile handling is impracticable or impossible. In particular, it facilitates the direct in vitro isolation of parasites from an infected host. Grether-Bühler, Yvonne verfasserin aut Kaminsky, Ronald verfasserin aut Brun, Reto verfasserin aut Enthalten in Parasitology research Berlin : Springer, 1928 88(2002), 2 vom: Feb., Seite 172-174 (DE-627)254638627 (DE-600)1462976-8 1432-1955 nnns volume:88 year:2002 number:2 month:02 pages:172-174 https://dx.doi.org/10.1007/s00436-001-0511-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2043 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2158 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2193 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2808 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 44.44 ASE 42.00 ASE AR 88 2002 2 02 172-174
allfieldsSound	10.1007/s00436-001-0511-5 doi (DE-627)SPR005885051 (SPR)s00436-001-0511-5-e DE-627 ger DE-627 rakwb eng 590 610 ASE 44.44 bkl 42.00 bkl Mäser, Pascal verfasserin aut An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract. Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G (60 $ µgml^{–1} $), kanamycin (100 $ µgml^{–1} $), chloramphenicol (10 $ µgml^{–1} $) and flucytosine (50 $ µgml^{–1} $). It is well tolerated by African and South American trypanosomes, Leishmania spp., Giardia duodenalis, Entamoeba histolytica and Plasmodium falciparum, and it provides an effective means for the treatment of contaminated cultures. The anti-contamination cocktail meets numerous prophylactic applications in which sterile handling is impracticable or impossible. In particular, it facilitates the direct in vitro isolation of parasites from an infected host. Grether-Bühler, Yvonne verfasserin aut Kaminsky, Ronald verfasserin aut Brun, Reto verfasserin aut Enthalten in Parasitology research Berlin : Springer, 1928 88(2002), 2 vom: Feb., Seite 172-174 (DE-627)254638627 (DE-600)1462976-8 1432-1955 nnns volume:88 year:2002 number:2 month:02 pages:172-174 https://dx.doi.org/10.1007/s00436-001-0511-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2043 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2158 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2193 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2808 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 44.44 ASE 42.00 ASE AR 88 2002 2 02 172-174
language	English
source	Enthalten in Parasitology research 88(2002), 2 vom: Feb., Seite 172-174 volume:88 year:2002 number:2 month:02 pages:172-174
sourceStr	Enthalten in Parasitology research 88(2002), 2 vom: Feb., Seite 172-174 volume:88 year:2002 number:2 month:02 pages:172-174
format_phy_str_mv	Article
institution	findex.gbv.de
dewey-raw	590
isfreeaccess_bool	false
container_title	Parasitology research
authorswithroles_txt_mv	Mäser, Pascal @@aut@@ Grether-Bühler, Yvonne @@aut@@ Kaminsky, Ronald @@aut@@ Brun, Reto @@aut@@
publishDateDaySort_date	2002-02-01T00:00:00Z
hierarchy_top_id	254638627
dewey-sort	3590
id	SPR005885051
language_de	englisch
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author	Mäser, Pascal
spellingShingle	Mäser, Pascal ddc 590 bkl 44.44 bkl 42.00 An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa
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topic_title	590 610 ASE 44.44 bkl 42.00 bkl An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa
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title	An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa
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title_full	An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa
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title_sort	anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa
title_auth	An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa
abstract	Abstract. Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G (60 $ µgml^{–1} $), kanamycin (100 $ µgml^{–1} $), chloramphenicol (10 $ µgml^{–1} $) and flucytosine (50 $ µgml^{–1} $). It is well tolerated by African and South American trypanosomes, Leishmania spp., Giardia duodenalis, Entamoeba histolytica and Plasmodium falciparum, and it provides an effective means for the treatment of contaminated cultures. The anti-contamination cocktail meets numerous prophylactic applications in which sterile handling is impracticable or impossible. In particular, it facilitates the direct in vitro isolation of parasites from an infected host.
abstractGer	Abstract. Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G (60 $ µgml^{–1} $), kanamycin (100 $ µgml^{–1} $), chloramphenicol (10 $ µgml^{–1} $) and flucytosine (50 $ µgml^{–1} $). It is well tolerated by African and South American trypanosomes, Leishmania spp., Giardia duodenalis, Entamoeba histolytica and Plasmodium falciparum, and it provides an effective means for the treatment of contaminated cultures. The anti-contamination cocktail meets numerous prophylactic applications in which sterile handling is impracticable or impossible. In particular, it facilitates the direct in vitro isolation of parasites from an infected host.
abstract_unstemmed	Abstract. Contamination by bacteria or fungi is a frequent problem with the in vitro isolation and propagation of protozoan parasites. We developed an antibiotic combination of broad anti-contaminant activity but minimal toxicity to protozoa. This anti-contamination cocktail consists of penicillin G (60 $ µgml^{–1} $), kanamycin (100 $ µgml^{–1} $), chloramphenicol (10 $ µgml^{–1} $) and flucytosine (50 $ µgml^{–1} $). It is well tolerated by African and South American trypanosomes, Leishmania spp., Giardia duodenalis, Entamoeba histolytica and Plasmodium falciparum, and it provides an effective means for the treatment of contaminated cultures. The anti-contamination cocktail meets numerous prophylactic applications in which sterile handling is impracticable or impossible. In particular, it facilitates the direct in vitro isolation of parasites from an infected host.
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title_short	An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa
url	https://dx.doi.org/10.1007/s00436-001-0511-5
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An anti-contamination cocktail for the in vitro isolation and cultivation of parasitic protozoa

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