Reticulocyte measurements in rat, dog and mouse whole blood samples using the Sysmex XT-2000iV
Abstract Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance....
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Mathers, Anne [verfasserIn] Evans, Gareth O. [verfasserIn] Bleby, John [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2011 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Comparative haematology international - London : Springer, 1991, 21(2011), 5 vom: 07. Jan., Seite 631-637 |
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| Übergeordnetes Werk: |
volume:21 ; year:2011 ; number:5 ; day:07 ; month:01 ; pages:631-637 |
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| DOI / URN: |
10.1007/s00580-010-1147-6 |
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| 520 | |a Abstract Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. It is important to establish baseline data before exploiting these measurements when investigating perturbations of erythropoiesis due to xenobiotics. We also investigated the correlations between the mean reticulocyte haemoglobin content (CHr), available on the Advia 120™ and the two parameters (Ret-Y and RBC-Y) available on XT-2000iV, as both of these measurements can be used to investigate iron deficiency anaemias. | ||
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| 650 | 4 | |a Dog blood |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Reticulocyte fractional counts |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Bleby, John |e verfasserin |4 aut | |
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10.1007/s00580-010-1147-6 doi (DE-627)SPR007218796 (SPR)s00580-010-1147-6-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE Mathers, Anne verfasserin aut Reticulocyte measurements in rat, dog and mouse whole blood samples using the Sysmex XT-2000iV 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. It is important to establish baseline data before exploiting these measurements when investigating perturbations of erythropoiesis due to xenobiotics. We also investigated the correlations between the mean reticulocyte haemoglobin content (CHr), available on the Advia 120™ and the two parameters (Ret-Y and RBC-Y) available on XT-2000iV, as both of these measurements can be used to investigate iron deficiency anaemias. Haematology analyser—Sysmex XT-2000iV (dpeaa)DE-He213 Advia 120™ (dpeaa)DE-He213 Rat blood (dpeaa)DE-He213 Mouse blood (dpeaa)DE-He213 Dog blood (dpeaa)DE-He213 Reticulocyte fractional counts (dpeaa)DE-He213 Evans, Gareth O. verfasserin aut Bleby, John verfasserin aut Enthalten in Comparative haematology international London : Springer, 1991 21(2011), 5 vom: 07. Jan., Seite 631-637 (DE-627)559430590 (DE-600)2413027-8 1433-2973 nnns volume:21 year:2011 number:5 day:07 month:01 pages:631-637 https://dx.doi.org/10.1007/s00580-010-1147-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 21 2011 5 07 01 631-637 |
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10.1007/s00580-010-1147-6 doi (DE-627)SPR007218796 (SPR)s00580-010-1147-6-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE Mathers, Anne verfasserin aut Reticulocyte measurements in rat, dog and mouse whole blood samples using the Sysmex XT-2000iV 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. It is important to establish baseline data before exploiting these measurements when investigating perturbations of erythropoiesis due to xenobiotics. We also investigated the correlations between the mean reticulocyte haemoglobin content (CHr), available on the Advia 120™ and the two parameters (Ret-Y and RBC-Y) available on XT-2000iV, as both of these measurements can be used to investigate iron deficiency anaemias. Haematology analyser—Sysmex XT-2000iV (dpeaa)DE-He213 Advia 120™ (dpeaa)DE-He213 Rat blood (dpeaa)DE-He213 Mouse blood (dpeaa)DE-He213 Dog blood (dpeaa)DE-He213 Reticulocyte fractional counts (dpeaa)DE-He213 Evans, Gareth O. verfasserin aut Bleby, John verfasserin aut Enthalten in Comparative haematology international London : Springer, 1991 21(2011), 5 vom: 07. Jan., Seite 631-637 (DE-627)559430590 (DE-600)2413027-8 1433-2973 nnns volume:21 year:2011 number:5 day:07 month:01 pages:631-637 https://dx.doi.org/10.1007/s00580-010-1147-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 21 2011 5 07 01 631-637 |
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10.1007/s00580-010-1147-6 doi (DE-627)SPR007218796 (SPR)s00580-010-1147-6-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE Mathers, Anne verfasserin aut Reticulocyte measurements in rat, dog and mouse whole blood samples using the Sysmex XT-2000iV 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. It is important to establish baseline data before exploiting these measurements when investigating perturbations of erythropoiesis due to xenobiotics. We also investigated the correlations between the mean reticulocyte haemoglobin content (CHr), available on the Advia 120™ and the two parameters (Ret-Y and RBC-Y) available on XT-2000iV, as both of these measurements can be used to investigate iron deficiency anaemias. Haematology analyser—Sysmex XT-2000iV (dpeaa)DE-He213 Advia 120™ (dpeaa)DE-He213 Rat blood (dpeaa)DE-He213 Mouse blood (dpeaa)DE-He213 Dog blood (dpeaa)DE-He213 Reticulocyte fractional counts (dpeaa)DE-He213 Evans, Gareth O. verfasserin aut Bleby, John verfasserin aut Enthalten in Comparative haematology international London : Springer, 1991 21(2011), 5 vom: 07. Jan., Seite 631-637 (DE-627)559430590 (DE-600)2413027-8 1433-2973 nnns volume:21 year:2011 number:5 day:07 month:01 pages:631-637 https://dx.doi.org/10.1007/s00580-010-1147-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 21 2011 5 07 01 631-637 |
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10.1007/s00580-010-1147-6 doi (DE-627)SPR007218796 (SPR)s00580-010-1147-6-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE Mathers, Anne verfasserin aut Reticulocyte measurements in rat, dog and mouse whole blood samples using the Sysmex XT-2000iV 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. It is important to establish baseline data before exploiting these measurements when investigating perturbations of erythropoiesis due to xenobiotics. We also investigated the correlations between the mean reticulocyte haemoglobin content (CHr), available on the Advia 120™ and the two parameters (Ret-Y and RBC-Y) available on XT-2000iV, as both of these measurements can be used to investigate iron deficiency anaemias. Haematology analyser—Sysmex XT-2000iV (dpeaa)DE-He213 Advia 120™ (dpeaa)DE-He213 Rat blood (dpeaa)DE-He213 Mouse blood (dpeaa)DE-He213 Dog blood (dpeaa)DE-He213 Reticulocyte fractional counts (dpeaa)DE-He213 Evans, Gareth O. verfasserin aut Bleby, John verfasserin aut Enthalten in Comparative haematology international London : Springer, 1991 21(2011), 5 vom: 07. Jan., Seite 631-637 (DE-627)559430590 (DE-600)2413027-8 1433-2973 nnns volume:21 year:2011 number:5 day:07 month:01 pages:631-637 https://dx.doi.org/10.1007/s00580-010-1147-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 21 2011 5 07 01 631-637 |
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10.1007/s00580-010-1147-6 doi (DE-627)SPR007218796 (SPR)s00580-010-1147-6-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE Mathers, Anne verfasserin aut Reticulocyte measurements in rat, dog and mouse whole blood samples using the Sysmex XT-2000iV 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. It is important to establish baseline data before exploiting these measurements when investigating perturbations of erythropoiesis due to xenobiotics. We also investigated the correlations between the mean reticulocyte haemoglobin content (CHr), available on the Advia 120™ and the two parameters (Ret-Y and RBC-Y) available on XT-2000iV, as both of these measurements can be used to investigate iron deficiency anaemias. Haematology analyser—Sysmex XT-2000iV (dpeaa)DE-He213 Advia 120™ (dpeaa)DE-He213 Rat blood (dpeaa)DE-He213 Mouse blood (dpeaa)DE-He213 Dog blood (dpeaa)DE-He213 Reticulocyte fractional counts (dpeaa)DE-He213 Evans, Gareth O. verfasserin aut Bleby, John verfasserin aut Enthalten in Comparative haematology international London : Springer, 1991 21(2011), 5 vom: 07. Jan., Seite 631-637 (DE-627)559430590 (DE-600)2413027-8 1433-2973 nnns volume:21 year:2011 number:5 day:07 month:01 pages:631-637 https://dx.doi.org/10.1007/s00580-010-1147-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 21 2011 5 07 01 631-637 |
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Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. 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Reticulocyte measurements in rat, dog and mouse whole blood samples using the Sysmex XT-2000iV |
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Abstract Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. It is important to establish baseline data before exploiting these measurements when investigating perturbations of erythropoiesis due to xenobiotics. We also investigated the correlations between the mean reticulocyte haemoglobin content (CHr), available on the Advia 120™ and the two parameters (Ret-Y and RBC-Y) available on XT-2000iV, as both of these measurements can be used to investigate iron deficiency anaemias. |
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Abstract Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. It is important to establish baseline data before exploiting these measurements when investigating perturbations of erythropoiesis due to xenobiotics. We also investigated the correlations between the mean reticulocyte haemoglobin content (CHr), available on the Advia 120™ and the two parameters (Ret-Y and RBC-Y) available on XT-2000iV, as both of these measurements can be used to investigate iron deficiency anaemias. |
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Abstract Immature reticulocyte measurements in peripheral blood are being used as an indicator of erythropoiesis. Some haematology analysers can now provide an indication of reticulocyte maturity by dividing the reticulocytes into three counting regions based on fluorescent intensity or absorbance. In this study, reticulocyte measurements were evaluated for three laboratory species—rat, dog and mouse—and we found the Sysmex XT-2000iV to give satisfactory analytical performance in terms of precision, carry-over between samples, linearity and sample stability. Using the XT-2000iV, results were obtained for reticulocyte absolute counts and the three regional subpopulations of reticulocytes, and these were compared with results obtained with the Siemens Advia 120™. The study highlighted differences between the analysers which may reflect the methodology of the Advia 120™ absorbance measurements and the fluorescence method used by the XT-2000iV. The different cell gating approaches and the lack of suitable calibration and control materials also contributes to these findings. It is important to establish baseline data before exploiting these measurements when investigating perturbations of erythropoiesis due to xenobiotics. We also investigated the correlations between the mean reticulocyte haemoglobin content (CHr), available on the Advia 120™ and the two parameters (Ret-Y and RBC-Y) available on XT-2000iV, as both of these measurements can be used to investigate iron deficiency anaemias. |
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