Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6
Summary. The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address t...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yang, A.-D. [verfasserIn] Barro, M. [verfasserIn] Gorziglia, M. I. [verfasserIn] Patton, J. T. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2003 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Archives of virology - Wien : Springer, 1939, 149(2003), 2 vom: 30. Okt., Seite 303-321 |
|---|---|
| Übergeordnetes Werk: |
volume:149 ; year:2003 ; number:2 ; day:30 ; month:10 ; pages:303-321 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00705-003-0211-9 |
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| Katalog-ID: |
SPR00737285X |
|---|
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| 035 | |a (DE-627)SPR00737285X | ||
| 035 | |a (SPR)s00705-003-0211-9-e | ||
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| 100 | 1 | |a Yang, A.-D. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 |
| 264 | 1 | |c 2003 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Summary. The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address the possibility that gene-specific enhancers are also contained in the untranslated regions (UTRs) of the rotavirus mRNAs, we used rabbit reticulocyte lysates to investigate the translation efficiencies of analog RNAs containing viral-specific 5′-and 3′-UTRs and the open reading frame for chloramphenicol acetyltransferase. These experiments combined with the analysis of full-length viral RNAs and RNAs containing 3′-truncations showed that a highly active enhancer was present near the 5′-end of the 139-nucleotide 3′-UTR of the gene 6 mRNA (3′-TEg6). The 3′-TEg6 represents a functionally independent enhancer, as no other portion of the gene 6 mRNA was required for its activity. The 3′-TEg6 differs significantly from the 3′-TE-con in that the gene 6-specific enhancer does not require viral protein for activity and is formed by a sequence unique to only one of the eleven viral mRNAs. Together, our findings suggest that the 3′-UTR of the gene 6 mRNA contains two TEs, one is gene-specific (3′-TEg6) and the other is common to nearly all rotavirus genes (3′-TE-con). The activity of the 3′-TEg6 is likely important for directing the efficient translation of the gene 6 mRNA at levels sufficient to provide the 780 copies of VP6 necessary for the assembly of each progeny virion. | ||
| 650 | 4 | |a Nonstructural Protein |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Progeny Virion |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Viral mRNAs |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Major Capsid Protein |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Rabbit Reticulocyte Lysate |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Barro, M. |e verfasserin |4 aut | |
| 700 | 1 | |a Gorziglia, M. I. |e verfasserin |4 aut | |
| 700 | 1 | |a Patton, J. T. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Archives of virology |d Wien : Springer, 1939 |g 149(2003), 2 vom: 30. Okt., Seite 303-321 |w (DE-627)253390168 |w (DE-600)1458460-8 |x 1432-8798 |7 nnns |
| 773 | 1 | 8 | |g volume:149 |g year:2003 |g number:2 |g day:30 |g month:10 |g pages:303-321 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s00705-003-0211-9 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a GBV_ILN_11 | ||
| 912 | |a GBV_ILN_20 | ||
| 912 | |a GBV_ILN_22 | ||
| 912 | |a GBV_ILN_23 | ||
| 912 | |a GBV_ILN_24 | ||
| 912 | |a GBV_ILN_31 | ||
| 912 | |a GBV_ILN_32 | ||
| 912 | |a GBV_ILN_39 | ||
| 912 | |a GBV_ILN_40 | ||
| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
| 912 | |a GBV_ILN_63 | ||
| 912 | |a GBV_ILN_69 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_73 | ||
| 912 | |a GBV_ILN_74 | ||
| 912 | |a GBV_ILN_90 | ||
| 912 | |a GBV_ILN_95 | ||
| 912 | |a GBV_ILN_100 | ||
| 912 | |a GBV_ILN_101 | ||
| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_138 | ||
| 912 | |a GBV_ILN_150 | ||
| 912 | |a GBV_ILN_151 | ||
| 912 | |a GBV_ILN_152 | ||
| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
| 912 | |a GBV_ILN_171 | ||
| 912 | |a GBV_ILN_187 | ||
| 912 | |a GBV_ILN_213 | ||
| 912 | |a GBV_ILN_224 | ||
| 912 | |a GBV_ILN_230 | ||
| 912 | |a GBV_ILN_250 | ||
| 912 | |a GBV_ILN_252 | ||
| 912 | |a GBV_ILN_267 | ||
| 912 | |a GBV_ILN_281 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_711 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2007 | ||
| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4328 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
| 912 | |a GBV_ILN_4700 | ||
| 936 | b | k | |a 42.32 |q ASE |
| 936 | b | k | |a 44.43 |q ASE |
| 951 | |a AR | ||
| 952 | |d 149 |j 2003 |e 2 |b 30 |c 10 |h 303-321 | ||
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2003 |
| allfields |
10.1007/s00705-003-0211-9 doi (DE-627)SPR00737285X (SPR)s00705-003-0211-9-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Yang, A.-D. verfasserin aut Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary. The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address the possibility that gene-specific enhancers are also contained in the untranslated regions (UTRs) of the rotavirus mRNAs, we used rabbit reticulocyte lysates to investigate the translation efficiencies of analog RNAs containing viral-specific 5′-and 3′-UTRs and the open reading frame for chloramphenicol acetyltransferase. These experiments combined with the analysis of full-length viral RNAs and RNAs containing 3′-truncations showed that a highly active enhancer was present near the 5′-end of the 139-nucleotide 3′-UTR of the gene 6 mRNA (3′-TEg6). The 3′-TEg6 represents a functionally independent enhancer, as no other portion of the gene 6 mRNA was required for its activity. The 3′-TEg6 differs significantly from the 3′-TE-con in that the gene 6-specific enhancer does not require viral protein for activity and is formed by a sequence unique to only one of the eleven viral mRNAs. Together, our findings suggest that the 3′-UTR of the gene 6 mRNA contains two TEs, one is gene-specific (3′-TEg6) and the other is common to nearly all rotavirus genes (3′-TE-con). The activity of the 3′-TEg6 is likely important for directing the efficient translation of the gene 6 mRNA at levels sufficient to provide the 780 copies of VP6 necessary for the assembly of each progeny virion. Nonstructural Protein (dpeaa)DE-He213 Progeny Virion (dpeaa)DE-He213 Viral mRNAs (dpeaa)DE-He213 Major Capsid Protein (dpeaa)DE-He213 Rabbit Reticulocyte Lysate (dpeaa)DE-He213 Barro, M. verfasserin aut Gorziglia, M. I. verfasserin aut Patton, J. T. verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 149(2003), 2 vom: 30. Okt., Seite 303-321 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:149 year:2003 number:2 day:30 month:10 pages:303-321 https://dx.doi.org/10.1007/s00705-003-0211-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 149 2003 2 30 10 303-321 |
| spelling |
10.1007/s00705-003-0211-9 doi (DE-627)SPR00737285X (SPR)s00705-003-0211-9-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Yang, A.-D. verfasserin aut Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary. The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address the possibility that gene-specific enhancers are also contained in the untranslated regions (UTRs) of the rotavirus mRNAs, we used rabbit reticulocyte lysates to investigate the translation efficiencies of analog RNAs containing viral-specific 5′-and 3′-UTRs and the open reading frame for chloramphenicol acetyltransferase. These experiments combined with the analysis of full-length viral RNAs and RNAs containing 3′-truncations showed that a highly active enhancer was present near the 5′-end of the 139-nucleotide 3′-UTR of the gene 6 mRNA (3′-TEg6). The 3′-TEg6 represents a functionally independent enhancer, as no other portion of the gene 6 mRNA was required for its activity. The 3′-TEg6 differs significantly from the 3′-TE-con in that the gene 6-specific enhancer does not require viral protein for activity and is formed by a sequence unique to only one of the eleven viral mRNAs. Together, our findings suggest that the 3′-UTR of the gene 6 mRNA contains two TEs, one is gene-specific (3′-TEg6) and the other is common to nearly all rotavirus genes (3′-TE-con). The activity of the 3′-TEg6 is likely important for directing the efficient translation of the gene 6 mRNA at levels sufficient to provide the 780 copies of VP6 necessary for the assembly of each progeny virion. Nonstructural Protein (dpeaa)DE-He213 Progeny Virion (dpeaa)DE-He213 Viral mRNAs (dpeaa)DE-He213 Major Capsid Protein (dpeaa)DE-He213 Rabbit Reticulocyte Lysate (dpeaa)DE-He213 Barro, M. verfasserin aut Gorziglia, M. I. verfasserin aut Patton, J. T. verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 149(2003), 2 vom: 30. Okt., Seite 303-321 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:149 year:2003 number:2 day:30 month:10 pages:303-321 https://dx.doi.org/10.1007/s00705-003-0211-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 149 2003 2 30 10 303-321 |
| allfields_unstemmed |
10.1007/s00705-003-0211-9 doi (DE-627)SPR00737285X (SPR)s00705-003-0211-9-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Yang, A.-D. verfasserin aut Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary. The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address the possibility that gene-specific enhancers are also contained in the untranslated regions (UTRs) of the rotavirus mRNAs, we used rabbit reticulocyte lysates to investigate the translation efficiencies of analog RNAs containing viral-specific 5′-and 3′-UTRs and the open reading frame for chloramphenicol acetyltransferase. These experiments combined with the analysis of full-length viral RNAs and RNAs containing 3′-truncations showed that a highly active enhancer was present near the 5′-end of the 139-nucleotide 3′-UTR of the gene 6 mRNA (3′-TEg6). The 3′-TEg6 represents a functionally independent enhancer, as no other portion of the gene 6 mRNA was required for its activity. The 3′-TEg6 differs significantly from the 3′-TE-con in that the gene 6-specific enhancer does not require viral protein for activity and is formed by a sequence unique to only one of the eleven viral mRNAs. Together, our findings suggest that the 3′-UTR of the gene 6 mRNA contains two TEs, one is gene-specific (3′-TEg6) and the other is common to nearly all rotavirus genes (3′-TE-con). The activity of the 3′-TEg6 is likely important for directing the efficient translation of the gene 6 mRNA at levels sufficient to provide the 780 copies of VP6 necessary for the assembly of each progeny virion. Nonstructural Protein (dpeaa)DE-He213 Progeny Virion (dpeaa)DE-He213 Viral mRNAs (dpeaa)DE-He213 Major Capsid Protein (dpeaa)DE-He213 Rabbit Reticulocyte Lysate (dpeaa)DE-He213 Barro, M. verfasserin aut Gorziglia, M. I. verfasserin aut Patton, J. T. verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 149(2003), 2 vom: 30. Okt., Seite 303-321 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:149 year:2003 number:2 day:30 month:10 pages:303-321 https://dx.doi.org/10.1007/s00705-003-0211-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 149 2003 2 30 10 303-321 |
| allfieldsGer |
10.1007/s00705-003-0211-9 doi (DE-627)SPR00737285X (SPR)s00705-003-0211-9-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Yang, A.-D. verfasserin aut Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary. The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address the possibility that gene-specific enhancers are also contained in the untranslated regions (UTRs) of the rotavirus mRNAs, we used rabbit reticulocyte lysates to investigate the translation efficiencies of analog RNAs containing viral-specific 5′-and 3′-UTRs and the open reading frame for chloramphenicol acetyltransferase. These experiments combined with the analysis of full-length viral RNAs and RNAs containing 3′-truncations showed that a highly active enhancer was present near the 5′-end of the 139-nucleotide 3′-UTR of the gene 6 mRNA (3′-TEg6). The 3′-TEg6 represents a functionally independent enhancer, as no other portion of the gene 6 mRNA was required for its activity. The 3′-TEg6 differs significantly from the 3′-TE-con in that the gene 6-specific enhancer does not require viral protein for activity and is formed by a sequence unique to only one of the eleven viral mRNAs. Together, our findings suggest that the 3′-UTR of the gene 6 mRNA contains two TEs, one is gene-specific (3′-TEg6) and the other is common to nearly all rotavirus genes (3′-TE-con). The activity of the 3′-TEg6 is likely important for directing the efficient translation of the gene 6 mRNA at levels sufficient to provide the 780 copies of VP6 necessary for the assembly of each progeny virion. Nonstructural Protein (dpeaa)DE-He213 Progeny Virion (dpeaa)DE-He213 Viral mRNAs (dpeaa)DE-He213 Major Capsid Protein (dpeaa)DE-He213 Rabbit Reticulocyte Lysate (dpeaa)DE-He213 Barro, M. verfasserin aut Gorziglia, M. I. verfasserin aut Patton, J. T. verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 149(2003), 2 vom: 30. Okt., Seite 303-321 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:149 year:2003 number:2 day:30 month:10 pages:303-321 https://dx.doi.org/10.1007/s00705-003-0211-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 149 2003 2 30 10 303-321 |
| allfieldsSound |
10.1007/s00705-003-0211-9 doi (DE-627)SPR00737285X (SPR)s00705-003-0211-9-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Yang, A.-D. verfasserin aut Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary. The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address the possibility that gene-specific enhancers are also contained in the untranslated regions (UTRs) of the rotavirus mRNAs, we used rabbit reticulocyte lysates to investigate the translation efficiencies of analog RNAs containing viral-specific 5′-and 3′-UTRs and the open reading frame for chloramphenicol acetyltransferase. These experiments combined with the analysis of full-length viral RNAs and RNAs containing 3′-truncations showed that a highly active enhancer was present near the 5′-end of the 139-nucleotide 3′-UTR of the gene 6 mRNA (3′-TEg6). The 3′-TEg6 represents a functionally independent enhancer, as no other portion of the gene 6 mRNA was required for its activity. The 3′-TEg6 differs significantly from the 3′-TE-con in that the gene 6-specific enhancer does not require viral protein for activity and is formed by a sequence unique to only one of the eleven viral mRNAs. Together, our findings suggest that the 3′-UTR of the gene 6 mRNA contains two TEs, one is gene-specific (3′-TEg6) and the other is common to nearly all rotavirus genes (3′-TE-con). The activity of the 3′-TEg6 is likely important for directing the efficient translation of the gene 6 mRNA at levels sufficient to provide the 780 copies of VP6 necessary for the assembly of each progeny virion. Nonstructural Protein (dpeaa)DE-He213 Progeny Virion (dpeaa)DE-He213 Viral mRNAs (dpeaa)DE-He213 Major Capsid Protein (dpeaa)DE-He213 Rabbit Reticulocyte Lysate (dpeaa)DE-He213 Barro, M. verfasserin aut Gorziglia, M. I. verfasserin aut Patton, J. T. verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 149(2003), 2 vom: 30. Okt., Seite 303-321 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:149 year:2003 number:2 day:30 month:10 pages:303-321 https://dx.doi.org/10.1007/s00705-003-0211-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 149 2003 2 30 10 303-321 |
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English |
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Enthalten in Archives of virology 149(2003), 2 vom: 30. Okt., Seite 303-321 volume:149 year:2003 number:2 day:30 month:10 pages:303-321 |
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Enthalten in Archives of virology 149(2003), 2 vom: 30. Okt., Seite 303-321 volume:149 year:2003 number:2 day:30 month:10 pages:303-321 |
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Nonstructural Protein Progeny Virion Viral mRNAs Major Capsid Protein Rabbit Reticulocyte Lysate |
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Yang, A.-D. @@aut@@ Barro, M. @@aut@@ Gorziglia, M. I. @@aut@@ Patton, J. T. @@aut@@ |
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The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address the possibility that gene-specific enhancers are also contained in the untranslated regions (UTRs) of the rotavirus mRNAs, we used rabbit reticulocyte lysates to investigate the translation efficiencies of analog RNAs containing viral-specific 5′-and 3′-UTRs and the open reading frame for chloramphenicol acetyltransferase. These experiments combined with the analysis of full-length viral RNAs and RNAs containing 3′-truncations showed that a highly active enhancer was present near the 5′-end of the 139-nucleotide 3′-UTR of the gene 6 mRNA (3′-TEg6). The 3′-TEg6 represents a functionally independent enhancer, as no other portion of the gene 6 mRNA was required for its activity. The 3′-TEg6 differs significantly from the 3′-TE-con in that the gene 6-specific enhancer does not require viral protein for activity and is formed by a sequence unique to only one of the eleven viral mRNAs. Together, our findings suggest that the 3′-UTR of the gene 6 mRNA contains two TEs, one is gene-specific (3′-TEg6) and the other is common to nearly all rotavirus genes (3′-TE-con). The activity of the 3′-TEg6 is likely important for directing the efficient translation of the gene 6 mRNA at levels sufficient to provide the 780 copies of VP6 necessary for the assembly of each progeny virion.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Nonstructural Protein</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Progeny Virion</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Viral mRNAs</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Major Capsid Protein</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Rabbit Reticulocyte Lysate</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Barro, M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gorziglia, M. I.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Patton, J. T.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Archives of virology</subfield><subfield code="d">Wien : Springer, 1939</subfield><subfield code="g">149(2003), 2 vom: 30. 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|
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Yang, A.-D. |
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Yang, A.-D. ddc 610 bkl 42.32 bkl 44.43 misc Nonstructural Protein misc Progeny Virion misc Viral mRNAs misc Major Capsid Protein misc Rabbit Reticulocyte Lysate Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 |
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610 ASE 42.32 bkl 44.43 bkl Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 Nonstructural Protein (dpeaa)DE-He213 Progeny Virion (dpeaa)DE-He213 Viral mRNAs (dpeaa)DE-He213 Major Capsid Protein (dpeaa)DE-He213 Rabbit Reticulocyte Lysate (dpeaa)DE-He213 |
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ddc 610 bkl 42.32 bkl 44.43 misc Nonstructural Protein misc Progeny Virion misc Viral mRNAs misc Major Capsid Protein misc Rabbit Reticulocyte Lysate |
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Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 |
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Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 |
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Yang, A.-D. Barro, M. Gorziglia, M. I. Patton, J. T. |
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Yang, A.-D. |
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10.1007/s00705-003-0211-9 |
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verfasserin |
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translation enhancer in the 3′-untranslated region of rotavirus gene 6 mrna promotes expression of the major capsid protein vp6 |
| title_auth |
Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 |
| abstract |
Summary. The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address the possibility that gene-specific enhancers are also contained in the untranslated regions (UTRs) of the rotavirus mRNAs, we used rabbit reticulocyte lysates to investigate the translation efficiencies of analog RNAs containing viral-specific 5′-and 3′-UTRs and the open reading frame for chloramphenicol acetyltransferase. These experiments combined with the analysis of full-length viral RNAs and RNAs containing 3′-truncations showed that a highly active enhancer was present near the 5′-end of the 139-nucleotide 3′-UTR of the gene 6 mRNA (3′-TEg6). The 3′-TEg6 represents a functionally independent enhancer, as no other portion of the gene 6 mRNA was required for its activity. The 3′-TEg6 differs significantly from the 3′-TE-con in that the gene 6-specific enhancer does not require viral protein for activity and is formed by a sequence unique to only one of the eleven viral mRNAs. Together, our findings suggest that the 3′-UTR of the gene 6 mRNA contains two TEs, one is gene-specific (3′-TEg6) and the other is common to nearly all rotavirus genes (3′-TE-con). The activity of the 3′-TEg6 is likely important for directing the efficient translation of the gene 6 mRNA at levels sufficient to provide the 780 copies of VP6 necessary for the assembly of each progeny virion. |
| abstractGer |
Summary. The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address the possibility that gene-specific enhancers are also contained in the untranslated regions (UTRs) of the rotavirus mRNAs, we used rabbit reticulocyte lysates to investigate the translation efficiencies of analog RNAs containing viral-specific 5′-and 3′-UTRs and the open reading frame for chloramphenicol acetyltransferase. These experiments combined with the analysis of full-length viral RNAs and RNAs containing 3′-truncations showed that a highly active enhancer was present near the 5′-end of the 139-nucleotide 3′-UTR of the gene 6 mRNA (3′-TEg6). The 3′-TEg6 represents a functionally independent enhancer, as no other portion of the gene 6 mRNA was required for its activity. The 3′-TEg6 differs significantly from the 3′-TE-con in that the gene 6-specific enhancer does not require viral protein for activity and is formed by a sequence unique to only one of the eleven viral mRNAs. Together, our findings suggest that the 3′-UTR of the gene 6 mRNA contains two TEs, one is gene-specific (3′-TEg6) and the other is common to nearly all rotavirus genes (3′-TE-con). The activity of the 3′-TEg6 is likely important for directing the efficient translation of the gene 6 mRNA at levels sufficient to provide the 780 copies of VP6 necessary for the assembly of each progeny virion. |
| abstract_unstemmed |
Summary. The eleven rotavirus mRNAs contain 5′-cap structures and most end with the 3′-consensus sequence 5′-UGACC-3′. The UGACC functions as a common translation enhancer (3′-TE-con) that upregulates viral protein expression through a process mediated by the nonstructural protein NSP3. To address the possibility that gene-specific enhancers are also contained in the untranslated regions (UTRs) of the rotavirus mRNAs, we used rabbit reticulocyte lysates to investigate the translation efficiencies of analog RNAs containing viral-specific 5′-and 3′-UTRs and the open reading frame for chloramphenicol acetyltransferase. These experiments combined with the analysis of full-length viral RNAs and RNAs containing 3′-truncations showed that a highly active enhancer was present near the 5′-end of the 139-nucleotide 3′-UTR of the gene 6 mRNA (3′-TEg6). The 3′-TEg6 represents a functionally independent enhancer, as no other portion of the gene 6 mRNA was required for its activity. The 3′-TEg6 differs significantly from the 3′-TE-con in that the gene 6-specific enhancer does not require viral protein for activity and is formed by a sequence unique to only one of the eleven viral mRNAs. Together, our findings suggest that the 3′-UTR of the gene 6 mRNA contains two TEs, one is gene-specific (3′-TEg6) and the other is common to nearly all rotavirus genes (3′-TE-con). The activity of the 3′-TEg6 is likely important for directing the efficient translation of the gene 6 mRNA at levels sufficient to provide the 780 copies of VP6 necessary for the assembly of each progeny virion. |
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Translation enhancer in the 3′-untranslated region of rotavirus gene 6 mRNA promotes expression of the major capsid protein VP6 |
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| score |
7.3999605 |