Molecular characterization and detection of recombinant isolates of potato virus Y from China
Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two type...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Hu, Xinxi [verfasserIn] He, Changzheng [verfasserIn] Xiao, Ya [verfasserIn] Xiong, Xingyao [verfasserIn] Nie, Xianzhou [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Archives of virology - Wien : Springer, 1939, 154(2009), 8 vom: 12. Juli, Seite 1303-1312 |
|---|---|
| Übergeordnetes Werk: |
volume:154 ; year:2009 ; number:8 ; day:12 ; month:07 ; pages:1303-1312 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00705-009-0448-z |
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| Katalog-ID: |
SPR007387466 |
|---|
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| 245 | 1 | 0 | |a Molecular characterization and detection of recombinant isolates of potato virus Y from China |
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| 520 | |a Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two types of recombinant PVY were identified in China for the first time. One resembled the European (Eu) type of potato tuber necrosis strain (Eu-$ PVY^{NTN} $), possessing three widely recognized recombinant joints (RJs 1–3) of the common strain ($ PVY^{O} $) and the Eu- type tobacco veinal necrosis strain (Eu-$ PVY^{N} $). The other, on the other hand, appeared to have only RJ1 and RJ2. The complete genome of a representative isolate, PVY-HN2, from the second type was subsequently sequenced. Comparison of the sequence of ‘HN2’ with those of $ PVY^{O} $ and Eu-$ PVY^{N} $ not only confirmed the recombinant nature of ‘HN2’ but also revealed the existence of three recombinant events in the isolate, similar to that in $ PVY^{NTN} $-Hun. However, the two isolates differed significantly at RJ1 ($ PVY^{NTN} $-Hun vs. HN2, nt 2419 vs. nt 2521) and RJ3 ($ PVY^{NTN} $-Hun vs. HN2, nt 9183 vs. nt 8572) and slightly at RJ2 ($ PVY^{NTN} $-Hun vs. HN2, nt 5844 vs. nt 5867). A primer pair was developed to facilitate the detection of the alternative RJ3. Using the newly and previously designed RJ primers, all targeted RJs were detected. Interestingly, tests of the available PVY samples indicated that two were doubly infected with both types of recombinant PVY, further confirming the effectiveness of the detection. Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that ‘HN2’ possesses a $ PVY^{O} $ serotype, a $ PVY^{N} $ pathotype in tobacco and a $ PVY^{NTN} $ pathotype in potato. | ||
| 650 | 4 | |a Potato Virus |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Foliar Symptom |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Moloney Murine Leukemia Virus Reverse Transcriptase |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Potato Isolate |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Recombinant Isolate |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a He, Changzheng |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Ya |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Xingyao |e verfasserin |4 aut | |
| 700 | 1 | |a Nie, Xianzhou |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Archives of virology |d Wien : Springer, 1939 |g 154(2009), 8 vom: 12. Juli, Seite 1303-1312 |w (DE-627)253390168 |w (DE-600)1458460-8 |x 1432-8798 |7 nnns |
| 773 | 1 | 8 | |g volume:154 |g year:2009 |g number:8 |g day:12 |g month:07 |g pages:1303-1312 |
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| 912 | |a GBV_ILN_2088 | ||
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| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
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| 912 | |a GBV_ILN_2116 | ||
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| 912 | |a GBV_ILN_2122 | ||
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10.1007/s00705-009-0448-z doi (DE-627)SPR007387466 (SPR)s00705-009-0448-z-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Hu, Xinxi verfasserin aut Molecular characterization and detection of recombinant isolates of potato virus Y from China 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two types of recombinant PVY were identified in China for the first time. One resembled the European (Eu) type of potato tuber necrosis strain (Eu-$ PVY^{NTN} $), possessing three widely recognized recombinant joints (RJs 1–3) of the common strain ($ PVY^{O} $) and the Eu- type tobacco veinal necrosis strain (Eu-$ PVY^{N} $). The other, on the other hand, appeared to have only RJ1 and RJ2. The complete genome of a representative isolate, PVY-HN2, from the second type was subsequently sequenced. Comparison of the sequence of ‘HN2’ with those of $ PVY^{O} $ and Eu-$ PVY^{N} $ not only confirmed the recombinant nature of ‘HN2’ but also revealed the existence of three recombinant events in the isolate, similar to that in $ PVY^{NTN} $-Hun. However, the two isolates differed significantly at RJ1 ($ PVY^{NTN} $-Hun vs. HN2, nt 2419 vs. nt 2521) and RJ3 ($ PVY^{NTN} $-Hun vs. HN2, nt 9183 vs. nt 8572) and slightly at RJ2 ($ PVY^{NTN} $-Hun vs. HN2, nt 5844 vs. nt 5867). A primer pair was developed to facilitate the detection of the alternative RJ3. Using the newly and previously designed RJ primers, all targeted RJs were detected. Interestingly, tests of the available PVY samples indicated that two were doubly infected with both types of recombinant PVY, further confirming the effectiveness of the detection. Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that ‘HN2’ possesses a $ PVY^{O} $ serotype, a $ PVY^{N} $ pathotype in tobacco and a $ PVY^{NTN} $ pathotype in potato. Potato Virus (dpeaa)DE-He213 Foliar Symptom (dpeaa)DE-He213 Moloney Murine Leukemia Virus Reverse Transcriptase (dpeaa)DE-He213 Potato Isolate (dpeaa)DE-He213 Recombinant Isolate (dpeaa)DE-He213 He, Changzheng verfasserin aut Xiao, Ya verfasserin aut Xiong, Xingyao verfasserin aut Nie, Xianzhou verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 154(2009), 8 vom: 12. Juli, Seite 1303-1312 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:154 year:2009 number:8 day:12 month:07 pages:1303-1312 https://dx.doi.org/10.1007/s00705-009-0448-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 154 2009 8 12 07 1303-1312 |
| spelling |
10.1007/s00705-009-0448-z doi (DE-627)SPR007387466 (SPR)s00705-009-0448-z-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Hu, Xinxi verfasserin aut Molecular characterization and detection of recombinant isolates of potato virus Y from China 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two types of recombinant PVY were identified in China for the first time. One resembled the European (Eu) type of potato tuber necrosis strain (Eu-$ PVY^{NTN} $), possessing three widely recognized recombinant joints (RJs 1–3) of the common strain ($ PVY^{O} $) and the Eu- type tobacco veinal necrosis strain (Eu-$ PVY^{N} $). The other, on the other hand, appeared to have only RJ1 and RJ2. The complete genome of a representative isolate, PVY-HN2, from the second type was subsequently sequenced. Comparison of the sequence of ‘HN2’ with those of $ PVY^{O} $ and Eu-$ PVY^{N} $ not only confirmed the recombinant nature of ‘HN2’ but also revealed the existence of three recombinant events in the isolate, similar to that in $ PVY^{NTN} $-Hun. However, the two isolates differed significantly at RJ1 ($ PVY^{NTN} $-Hun vs. HN2, nt 2419 vs. nt 2521) and RJ3 ($ PVY^{NTN} $-Hun vs. HN2, nt 9183 vs. nt 8572) and slightly at RJ2 ($ PVY^{NTN} $-Hun vs. HN2, nt 5844 vs. nt 5867). A primer pair was developed to facilitate the detection of the alternative RJ3. Using the newly and previously designed RJ primers, all targeted RJs were detected. Interestingly, tests of the available PVY samples indicated that two were doubly infected with both types of recombinant PVY, further confirming the effectiveness of the detection. Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that ‘HN2’ possesses a $ PVY^{O} $ serotype, a $ PVY^{N} $ pathotype in tobacco and a $ PVY^{NTN} $ pathotype in potato. Potato Virus (dpeaa)DE-He213 Foliar Symptom (dpeaa)DE-He213 Moloney Murine Leukemia Virus Reverse Transcriptase (dpeaa)DE-He213 Potato Isolate (dpeaa)DE-He213 Recombinant Isolate (dpeaa)DE-He213 He, Changzheng verfasserin aut Xiao, Ya verfasserin aut Xiong, Xingyao verfasserin aut Nie, Xianzhou verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 154(2009), 8 vom: 12. Juli, Seite 1303-1312 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:154 year:2009 number:8 day:12 month:07 pages:1303-1312 https://dx.doi.org/10.1007/s00705-009-0448-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 154 2009 8 12 07 1303-1312 |
| allfields_unstemmed |
10.1007/s00705-009-0448-z doi (DE-627)SPR007387466 (SPR)s00705-009-0448-z-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Hu, Xinxi verfasserin aut Molecular characterization and detection of recombinant isolates of potato virus Y from China 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two types of recombinant PVY were identified in China for the first time. One resembled the European (Eu) type of potato tuber necrosis strain (Eu-$ PVY^{NTN} $), possessing three widely recognized recombinant joints (RJs 1–3) of the common strain ($ PVY^{O} $) and the Eu- type tobacco veinal necrosis strain (Eu-$ PVY^{N} $). The other, on the other hand, appeared to have only RJ1 and RJ2. The complete genome of a representative isolate, PVY-HN2, from the second type was subsequently sequenced. Comparison of the sequence of ‘HN2’ with those of $ PVY^{O} $ and Eu-$ PVY^{N} $ not only confirmed the recombinant nature of ‘HN2’ but also revealed the existence of three recombinant events in the isolate, similar to that in $ PVY^{NTN} $-Hun. However, the two isolates differed significantly at RJ1 ($ PVY^{NTN} $-Hun vs. HN2, nt 2419 vs. nt 2521) and RJ3 ($ PVY^{NTN} $-Hun vs. HN2, nt 9183 vs. nt 8572) and slightly at RJ2 ($ PVY^{NTN} $-Hun vs. HN2, nt 5844 vs. nt 5867). A primer pair was developed to facilitate the detection of the alternative RJ3. Using the newly and previously designed RJ primers, all targeted RJs were detected. Interestingly, tests of the available PVY samples indicated that two were doubly infected with both types of recombinant PVY, further confirming the effectiveness of the detection. Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that ‘HN2’ possesses a $ PVY^{O} $ serotype, a $ PVY^{N} $ pathotype in tobacco and a $ PVY^{NTN} $ pathotype in potato. Potato Virus (dpeaa)DE-He213 Foliar Symptom (dpeaa)DE-He213 Moloney Murine Leukemia Virus Reverse Transcriptase (dpeaa)DE-He213 Potato Isolate (dpeaa)DE-He213 Recombinant Isolate (dpeaa)DE-He213 He, Changzheng verfasserin aut Xiao, Ya verfasserin aut Xiong, Xingyao verfasserin aut Nie, Xianzhou verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 154(2009), 8 vom: 12. Juli, Seite 1303-1312 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:154 year:2009 number:8 day:12 month:07 pages:1303-1312 https://dx.doi.org/10.1007/s00705-009-0448-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 154 2009 8 12 07 1303-1312 |
| allfieldsGer |
10.1007/s00705-009-0448-z doi (DE-627)SPR007387466 (SPR)s00705-009-0448-z-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Hu, Xinxi verfasserin aut Molecular characterization and detection of recombinant isolates of potato virus Y from China 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two types of recombinant PVY were identified in China for the first time. One resembled the European (Eu) type of potato tuber necrosis strain (Eu-$ PVY^{NTN} $), possessing three widely recognized recombinant joints (RJs 1–3) of the common strain ($ PVY^{O} $) and the Eu- type tobacco veinal necrosis strain (Eu-$ PVY^{N} $). The other, on the other hand, appeared to have only RJ1 and RJ2. The complete genome of a representative isolate, PVY-HN2, from the second type was subsequently sequenced. Comparison of the sequence of ‘HN2’ with those of $ PVY^{O} $ and Eu-$ PVY^{N} $ not only confirmed the recombinant nature of ‘HN2’ but also revealed the existence of three recombinant events in the isolate, similar to that in $ PVY^{NTN} $-Hun. However, the two isolates differed significantly at RJ1 ($ PVY^{NTN} $-Hun vs. HN2, nt 2419 vs. nt 2521) and RJ3 ($ PVY^{NTN} $-Hun vs. HN2, nt 9183 vs. nt 8572) and slightly at RJ2 ($ PVY^{NTN} $-Hun vs. HN2, nt 5844 vs. nt 5867). A primer pair was developed to facilitate the detection of the alternative RJ3. Using the newly and previously designed RJ primers, all targeted RJs were detected. Interestingly, tests of the available PVY samples indicated that two were doubly infected with both types of recombinant PVY, further confirming the effectiveness of the detection. Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that ‘HN2’ possesses a $ PVY^{O} $ serotype, a $ PVY^{N} $ pathotype in tobacco and a $ PVY^{NTN} $ pathotype in potato. Potato Virus (dpeaa)DE-He213 Foliar Symptom (dpeaa)DE-He213 Moloney Murine Leukemia Virus Reverse Transcriptase (dpeaa)DE-He213 Potato Isolate (dpeaa)DE-He213 Recombinant Isolate (dpeaa)DE-He213 He, Changzheng verfasserin aut Xiao, Ya verfasserin aut Xiong, Xingyao verfasserin aut Nie, Xianzhou verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 154(2009), 8 vom: 12. Juli, Seite 1303-1312 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:154 year:2009 number:8 day:12 month:07 pages:1303-1312 https://dx.doi.org/10.1007/s00705-009-0448-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 154 2009 8 12 07 1303-1312 |
| allfieldsSound |
10.1007/s00705-009-0448-z doi (DE-627)SPR007387466 (SPR)s00705-009-0448-z-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Hu, Xinxi verfasserin aut Molecular characterization and detection of recombinant isolates of potato virus Y from China 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two types of recombinant PVY were identified in China for the first time. One resembled the European (Eu) type of potato tuber necrosis strain (Eu-$ PVY^{NTN} $), possessing three widely recognized recombinant joints (RJs 1–3) of the common strain ($ PVY^{O} $) and the Eu- type tobacco veinal necrosis strain (Eu-$ PVY^{N} $). The other, on the other hand, appeared to have only RJ1 and RJ2. The complete genome of a representative isolate, PVY-HN2, from the second type was subsequently sequenced. Comparison of the sequence of ‘HN2’ with those of $ PVY^{O} $ and Eu-$ PVY^{N} $ not only confirmed the recombinant nature of ‘HN2’ but also revealed the existence of three recombinant events in the isolate, similar to that in $ PVY^{NTN} $-Hun. However, the two isolates differed significantly at RJ1 ($ PVY^{NTN} $-Hun vs. HN2, nt 2419 vs. nt 2521) and RJ3 ($ PVY^{NTN} $-Hun vs. HN2, nt 9183 vs. nt 8572) and slightly at RJ2 ($ PVY^{NTN} $-Hun vs. HN2, nt 5844 vs. nt 5867). A primer pair was developed to facilitate the detection of the alternative RJ3. Using the newly and previously designed RJ primers, all targeted RJs were detected. Interestingly, tests of the available PVY samples indicated that two were doubly infected with both types of recombinant PVY, further confirming the effectiveness of the detection. Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that ‘HN2’ possesses a $ PVY^{O} $ serotype, a $ PVY^{N} $ pathotype in tobacco and a $ PVY^{NTN} $ pathotype in potato. Potato Virus (dpeaa)DE-He213 Foliar Symptom (dpeaa)DE-He213 Moloney Murine Leukemia Virus Reverse Transcriptase (dpeaa)DE-He213 Potato Isolate (dpeaa)DE-He213 Recombinant Isolate (dpeaa)DE-He213 He, Changzheng verfasserin aut Xiao, Ya verfasserin aut Xiong, Xingyao verfasserin aut Nie, Xianzhou verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 154(2009), 8 vom: 12. Juli, Seite 1303-1312 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:154 year:2009 number:8 day:12 month:07 pages:1303-1312 https://dx.doi.org/10.1007/s00705-009-0448-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 154 2009 8 12 07 1303-1312 |
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English |
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Enthalten in Archives of virology 154(2009), 8 vom: 12. Juli, Seite 1303-1312 volume:154 year:2009 number:8 day:12 month:07 pages:1303-1312 |
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Enthalten in Archives of virology 154(2009), 8 vom: 12. Juli, Seite 1303-1312 volume:154 year:2009 number:8 day:12 month:07 pages:1303-1312 |
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Potato Virus Foliar Symptom Moloney Murine Leukemia Virus Reverse Transcriptase Potato Isolate Recombinant Isolate |
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Hu, Xinxi @@aut@@ He, Changzheng @@aut@@ Xiao, Ya @@aut@@ Xiong, Xingyao @@aut@@ Nie, Xianzhou @@aut@@ |
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2009-07-12T00:00:00Z |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR007387466</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519170609.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201005s2009 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00705-009-0448-z</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR007387466</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00705-009-0448-z-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.32</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.43</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hu, Xinxi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Molecular characterization and detection of recombinant isolates of potato virus Y from China</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2009</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two types of recombinant PVY were identified in China for the first time. One resembled the European (Eu) type of potato tuber necrosis strain (Eu-$ PVY^{NTN} $), possessing three widely recognized recombinant joints (RJs 1–3) of the common strain ($ PVY^{O} $) and the Eu- type tobacco veinal necrosis strain (Eu-$ PVY^{N} $). The other, on the other hand, appeared to have only RJ1 and RJ2. The complete genome of a representative isolate, PVY-HN2, from the second type was subsequently sequenced. Comparison of the sequence of ‘HN2’ with those of $ PVY^{O} $ and Eu-$ PVY^{N} $ not only confirmed the recombinant nature of ‘HN2’ but also revealed the existence of three recombinant events in the isolate, similar to that in $ PVY^{NTN} $-Hun. However, the two isolates differed significantly at RJ1 ($ PVY^{NTN} $-Hun vs. HN2, nt 2419 vs. nt 2521) and RJ3 ($ PVY^{NTN} $-Hun vs. HN2, nt 9183 vs. nt 8572) and slightly at RJ2 ($ PVY^{NTN} $-Hun vs. HN2, nt 5844 vs. nt 5867). A primer pair was developed to facilitate the detection of the alternative RJ3. Using the newly and previously designed RJ primers, all targeted RJs were detected. Interestingly, tests of the available PVY samples indicated that two were doubly infected with both types of recombinant PVY, further confirming the effectiveness of the detection. Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that ‘HN2’ possesses a $ PVY^{O} $ serotype, a $ PVY^{N} $ pathotype in tobacco and a $ PVY^{NTN} $ pathotype in potato.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Potato Virus</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Foliar Symptom</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Moloney Murine Leukemia Virus Reverse Transcriptase</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Potato Isolate</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Recombinant Isolate</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">He, Changzheng</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xiao, Ya</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xiong, Xingyao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Nie, Xianzhou</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Archives of virology</subfield><subfield code="d">Wien : Springer, 1939</subfield><subfield code="g">154(2009), 8 vom: 12. 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|
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Hu, Xinxi |
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Hu, Xinxi ddc 610 bkl 42.32 bkl 44.43 misc Potato Virus misc Foliar Symptom misc Moloney Murine Leukemia Virus Reverse Transcriptase misc Potato Isolate misc Recombinant Isolate Molecular characterization and detection of recombinant isolates of potato virus Y from China |
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610 ASE 42.32 bkl 44.43 bkl Molecular characterization and detection of recombinant isolates of potato virus Y from China Potato Virus (dpeaa)DE-He213 Foliar Symptom (dpeaa)DE-He213 Moloney Murine Leukemia Virus Reverse Transcriptase (dpeaa)DE-He213 Potato Isolate (dpeaa)DE-He213 Recombinant Isolate (dpeaa)DE-He213 |
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ddc 610 bkl 42.32 bkl 44.43 misc Potato Virus misc Foliar Symptom misc Moloney Murine Leukemia Virus Reverse Transcriptase misc Potato Isolate misc Recombinant Isolate |
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ddc 610 bkl 42.32 bkl 44.43 misc Potato Virus misc Foliar Symptom misc Moloney Murine Leukemia Virus Reverse Transcriptase misc Potato Isolate misc Recombinant Isolate |
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Molecular characterization and detection of recombinant isolates of potato virus Y from China |
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Molecular characterization and detection of recombinant isolates of potato virus Y from China |
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Hu, Xinxi |
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Archives of virology |
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Hu, Xinxi He, Changzheng Xiao, Ya Xiong, Xingyao Nie, Xianzhou |
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molecular characterization and detection of recombinant isolates of potato virus y from china |
| title_auth |
Molecular characterization and detection of recombinant isolates of potato virus Y from China |
| abstract |
Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two types of recombinant PVY were identified in China for the first time. One resembled the European (Eu) type of potato tuber necrosis strain (Eu-$ PVY^{NTN} $), possessing three widely recognized recombinant joints (RJs 1–3) of the common strain ($ PVY^{O} $) and the Eu- type tobacco veinal necrosis strain (Eu-$ PVY^{N} $). The other, on the other hand, appeared to have only RJ1 and RJ2. The complete genome of a representative isolate, PVY-HN2, from the second type was subsequently sequenced. Comparison of the sequence of ‘HN2’ with those of $ PVY^{O} $ and Eu-$ PVY^{N} $ not only confirmed the recombinant nature of ‘HN2’ but also revealed the existence of three recombinant events in the isolate, similar to that in $ PVY^{NTN} $-Hun. However, the two isolates differed significantly at RJ1 ($ PVY^{NTN} $-Hun vs. HN2, nt 2419 vs. nt 2521) and RJ3 ($ PVY^{NTN} $-Hun vs. HN2, nt 9183 vs. nt 8572) and slightly at RJ2 ($ PVY^{NTN} $-Hun vs. HN2, nt 5844 vs. nt 5867). A primer pair was developed to facilitate the detection of the alternative RJ3. Using the newly and previously designed RJ primers, all targeted RJs were detected. Interestingly, tests of the available PVY samples indicated that two were doubly infected with both types of recombinant PVY, further confirming the effectiveness of the detection. Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that ‘HN2’ possesses a $ PVY^{O} $ serotype, a $ PVY^{N} $ pathotype in tobacco and a $ PVY^{NTN} $ pathotype in potato. |
| abstractGer |
Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two types of recombinant PVY were identified in China for the first time. One resembled the European (Eu) type of potato tuber necrosis strain (Eu-$ PVY^{NTN} $), possessing three widely recognized recombinant joints (RJs 1–3) of the common strain ($ PVY^{O} $) and the Eu- type tobacco veinal necrosis strain (Eu-$ PVY^{N} $). The other, on the other hand, appeared to have only RJ1 and RJ2. The complete genome of a representative isolate, PVY-HN2, from the second type was subsequently sequenced. Comparison of the sequence of ‘HN2’ with those of $ PVY^{O} $ and Eu-$ PVY^{N} $ not only confirmed the recombinant nature of ‘HN2’ but also revealed the existence of three recombinant events in the isolate, similar to that in $ PVY^{NTN} $-Hun. However, the two isolates differed significantly at RJ1 ($ PVY^{NTN} $-Hun vs. HN2, nt 2419 vs. nt 2521) and RJ3 ($ PVY^{NTN} $-Hun vs. HN2, nt 9183 vs. nt 8572) and slightly at RJ2 ($ PVY^{NTN} $-Hun vs. HN2, nt 5844 vs. nt 5867). A primer pair was developed to facilitate the detection of the alternative RJ3. Using the newly and previously designed RJ primers, all targeted RJs were detected. Interestingly, tests of the available PVY samples indicated that two were doubly infected with both types of recombinant PVY, further confirming the effectiveness of the detection. Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that ‘HN2’ possesses a $ PVY^{O} $ serotype, a $ PVY^{N} $ pathotype in tobacco and a $ PVY^{NTN} $ pathotype in potato. |
| abstract_unstemmed |
Abstract Although potato virus Y (PVY) is one of the most economically important pathogens of potatoes in China, few studies have been carried out to characterize the virus in that country. Using reverse transcription-polymerase chain reaction (RT-PCR)-based genotyping developed previously, two types of recombinant PVY were identified in China for the first time. One resembled the European (Eu) type of potato tuber necrosis strain (Eu-$ PVY^{NTN} $), possessing three widely recognized recombinant joints (RJs 1–3) of the common strain ($ PVY^{O} $) and the Eu- type tobacco veinal necrosis strain (Eu-$ PVY^{N} $). The other, on the other hand, appeared to have only RJ1 and RJ2. The complete genome of a representative isolate, PVY-HN2, from the second type was subsequently sequenced. Comparison of the sequence of ‘HN2’ with those of $ PVY^{O} $ and Eu-$ PVY^{N} $ not only confirmed the recombinant nature of ‘HN2’ but also revealed the existence of three recombinant events in the isolate, similar to that in $ PVY^{NTN} $-Hun. However, the two isolates differed significantly at RJ1 ($ PVY^{NTN} $-Hun vs. HN2, nt 2419 vs. nt 2521) and RJ3 ($ PVY^{NTN} $-Hun vs. HN2, nt 9183 vs. nt 8572) and slightly at RJ2 ($ PVY^{NTN} $-Hun vs. HN2, nt 5844 vs. nt 5867). A primer pair was developed to facilitate the detection of the alternative RJ3. Using the newly and previously designed RJ primers, all targeted RJs were detected. Interestingly, tests of the available PVY samples indicated that two were doubly infected with both types of recombinant PVY, further confirming the effectiveness of the detection. Further analysis of these samples using enzyme-linked immunosorbent assay and bioassay revealed that ‘HN2’ possesses a $ PVY^{O} $ serotype, a $ PVY^{N} $ pathotype in tobacco and a $ PVY^{NTN} $ pathotype in potato. |
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Molecular characterization and detection of recombinant isolates of potato virus Y from China |
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He, Changzheng Xiao, Ya Xiong, Xingyao Nie, Xianzhou |
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| score |
7.3996973 |