Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens
Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using exp...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zhang, Weijun [verfasserIn] Huang, Qinghua [verfasserIn] Lu, Mei [verfasserIn] Zhu, Fengzhu [verfasserIn] Huang, Yan-yan [verfasserIn] Yang, Shao-hua [verfasserIn] Kong, Zhengjie [verfasserIn] Zhang, Xiu-mei [verfasserIn] Xu, Chuan-tian [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Schlagwörter: |
Major Histocompatibility Complex |
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| Übergeordnetes Werk: |
Enthalten in: Archives of virology - Wien : Springer, 1939, 161(2016), 11 vom: 12. Aug., Seite 3081-3093 |
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| Übergeordnetes Werk: |
volume:161 ; year:2016 ; number:11 ; day:12 ; month:08 ; pages:3081-3093 |
| Links: |
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| DOI / URN: |
10.1007/s00705-016-3013-6 |
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| Katalog-ID: |
SPR007411715 |
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| 100 | 1 | |a Zhang, Weijun |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells. Antibodies against NP in pCAGGS-NP-inoculated specific-pathogen-free chickens were detected by enzyme-linked immunosorbent assay (ELISA). Interferon γ (IFN-γ) mRNA was quantified, and IFN-γ production was evaluated using quantitative reverse transcription PCR and capture ELISA, respectively. $ CD8^{+} $ T-lymphocyte proliferation was detected using flow cytometric analysis. The BF2*15 MHC class I binding motif “x-Arg/Lys-x-x-x-Arg/Lys” was explored. Quantification of chicken IFN-γ mRNA, evaluation of IFN-γ production, and measurement of $ CD8^{+} $ T-lymphocyte proliferation confirmed that the peptide $ NP_{67–74} $ of H5N1 was the BF2*15 MHC-class-I-restricted CTL epitope. | ||
| 650 | 4 | |a Major Histocompatibility Complex |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Major Histocompatibility Complex Class |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Molecular Docking |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a H5N1 Virus |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Infectious Bronchitis Virus |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Huang, Qinghua |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Mei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Fengzhu |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Yan-yan |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Shao-hua |e verfasserin |4 aut | |
| 700 | 1 | |a Kong, Zhengjie |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiu-mei |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Chuan-tian |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Archives of virology |d Wien : Springer, 1939 |g 161(2016), 11 vom: 12. Aug., Seite 3081-3093 |w (DE-627)253390168 |w (DE-600)1458460-8 |x 1432-8798 |7 nnns |
| 773 | 1 | 8 | |g volume:161 |g year:2016 |g number:11 |g day:12 |g month:08 |g pages:3081-3093 |
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| 912 | |a GBV_ILN_39 | ||
| 912 | |a GBV_ILN_40 | ||
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| 912 | |a GBV_ILN_74 | ||
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| 912 | |a GBV_ILN_100 | ||
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| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_138 | ||
| 912 | |a GBV_ILN_150 | ||
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| 912 | |a GBV_ILN_252 | ||
| 912 | |a GBV_ILN_267 | ||
| 912 | |a GBV_ILN_281 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_711 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2007 | ||
| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4328 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
| 912 | |a GBV_ILN_4700 | ||
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| allfields |
10.1007/s00705-016-3013-6 doi (DE-627)SPR007411715 (SPR)s00705-016-3013-6-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Zhang, Weijun verfasserin aut Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells. Antibodies against NP in pCAGGS-NP-inoculated specific-pathogen-free chickens were detected by enzyme-linked immunosorbent assay (ELISA). Interferon γ (IFN-γ) mRNA was quantified, and IFN-γ production was evaluated using quantitative reverse transcription PCR and capture ELISA, respectively. $ CD8^{+} $ T-lymphocyte proliferation was detected using flow cytometric analysis. The BF2*15 MHC class I binding motif “x-Arg/Lys-x-x-x-Arg/Lys” was explored. Quantification of chicken IFN-γ mRNA, evaluation of IFN-γ production, and measurement of $ CD8^{+} $ T-lymphocyte proliferation confirmed that the peptide $ NP_{67–74} $ of H5N1 was the BF2*15 MHC-class-I-restricted CTL epitope. Major Histocompatibility Complex (dpeaa)DE-He213 Major Histocompatibility Complex Class (dpeaa)DE-He213 Molecular Docking (dpeaa)DE-He213 H5N1 Virus (dpeaa)DE-He213 Infectious Bronchitis Virus (dpeaa)DE-He213 Huang, Qinghua verfasserin aut Lu, Mei verfasserin aut Zhu, Fengzhu verfasserin aut Huang, Yan-yan verfasserin aut Yang, Shao-hua verfasserin aut Kong, Zhengjie verfasserin aut Zhang, Xiu-mei verfasserin aut Xu, Chuan-tian verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 161(2016), 11 vom: 12. Aug., Seite 3081-3093 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:161 year:2016 number:11 day:12 month:08 pages:3081-3093 https://dx.doi.org/10.1007/s00705-016-3013-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 161 2016 11 12 08 3081-3093 |
| spelling |
10.1007/s00705-016-3013-6 doi (DE-627)SPR007411715 (SPR)s00705-016-3013-6-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Zhang, Weijun verfasserin aut Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells. Antibodies against NP in pCAGGS-NP-inoculated specific-pathogen-free chickens were detected by enzyme-linked immunosorbent assay (ELISA). Interferon γ (IFN-γ) mRNA was quantified, and IFN-γ production was evaluated using quantitative reverse transcription PCR and capture ELISA, respectively. $ CD8^{+} $ T-lymphocyte proliferation was detected using flow cytometric analysis. The BF2*15 MHC class I binding motif “x-Arg/Lys-x-x-x-Arg/Lys” was explored. Quantification of chicken IFN-γ mRNA, evaluation of IFN-γ production, and measurement of $ CD8^{+} $ T-lymphocyte proliferation confirmed that the peptide $ NP_{67–74} $ of H5N1 was the BF2*15 MHC-class-I-restricted CTL epitope. Major Histocompatibility Complex (dpeaa)DE-He213 Major Histocompatibility Complex Class (dpeaa)DE-He213 Molecular Docking (dpeaa)DE-He213 H5N1 Virus (dpeaa)DE-He213 Infectious Bronchitis Virus (dpeaa)DE-He213 Huang, Qinghua verfasserin aut Lu, Mei verfasserin aut Zhu, Fengzhu verfasserin aut Huang, Yan-yan verfasserin aut Yang, Shao-hua verfasserin aut Kong, Zhengjie verfasserin aut Zhang, Xiu-mei verfasserin aut Xu, Chuan-tian verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 161(2016), 11 vom: 12. Aug., Seite 3081-3093 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:161 year:2016 number:11 day:12 month:08 pages:3081-3093 https://dx.doi.org/10.1007/s00705-016-3013-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 161 2016 11 12 08 3081-3093 |
| allfields_unstemmed |
10.1007/s00705-016-3013-6 doi (DE-627)SPR007411715 (SPR)s00705-016-3013-6-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Zhang, Weijun verfasserin aut Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells. Antibodies against NP in pCAGGS-NP-inoculated specific-pathogen-free chickens were detected by enzyme-linked immunosorbent assay (ELISA). Interferon γ (IFN-γ) mRNA was quantified, and IFN-γ production was evaluated using quantitative reverse transcription PCR and capture ELISA, respectively. $ CD8^{+} $ T-lymphocyte proliferation was detected using flow cytometric analysis. The BF2*15 MHC class I binding motif “x-Arg/Lys-x-x-x-Arg/Lys” was explored. Quantification of chicken IFN-γ mRNA, evaluation of IFN-γ production, and measurement of $ CD8^{+} $ T-lymphocyte proliferation confirmed that the peptide $ NP_{67–74} $ of H5N1 was the BF2*15 MHC-class-I-restricted CTL epitope. Major Histocompatibility Complex (dpeaa)DE-He213 Major Histocompatibility Complex Class (dpeaa)DE-He213 Molecular Docking (dpeaa)DE-He213 H5N1 Virus (dpeaa)DE-He213 Infectious Bronchitis Virus (dpeaa)DE-He213 Huang, Qinghua verfasserin aut Lu, Mei verfasserin aut Zhu, Fengzhu verfasserin aut Huang, Yan-yan verfasserin aut Yang, Shao-hua verfasserin aut Kong, Zhengjie verfasserin aut Zhang, Xiu-mei verfasserin aut Xu, Chuan-tian verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 161(2016), 11 vom: 12. Aug., Seite 3081-3093 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:161 year:2016 number:11 day:12 month:08 pages:3081-3093 https://dx.doi.org/10.1007/s00705-016-3013-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 161 2016 11 12 08 3081-3093 |
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10.1007/s00705-016-3013-6 doi (DE-627)SPR007411715 (SPR)s00705-016-3013-6-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Zhang, Weijun verfasserin aut Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells. Antibodies against NP in pCAGGS-NP-inoculated specific-pathogen-free chickens were detected by enzyme-linked immunosorbent assay (ELISA). Interferon γ (IFN-γ) mRNA was quantified, and IFN-γ production was evaluated using quantitative reverse transcription PCR and capture ELISA, respectively. $ CD8^{+} $ T-lymphocyte proliferation was detected using flow cytometric analysis. The BF2*15 MHC class I binding motif “x-Arg/Lys-x-x-x-Arg/Lys” was explored. Quantification of chicken IFN-γ mRNA, evaluation of IFN-γ production, and measurement of $ CD8^{+} $ T-lymphocyte proliferation confirmed that the peptide $ NP_{67–74} $ of H5N1 was the BF2*15 MHC-class-I-restricted CTL epitope. Major Histocompatibility Complex (dpeaa)DE-He213 Major Histocompatibility Complex Class (dpeaa)DE-He213 Molecular Docking (dpeaa)DE-He213 H5N1 Virus (dpeaa)DE-He213 Infectious Bronchitis Virus (dpeaa)DE-He213 Huang, Qinghua verfasserin aut Lu, Mei verfasserin aut Zhu, Fengzhu verfasserin aut Huang, Yan-yan verfasserin aut Yang, Shao-hua verfasserin aut Kong, Zhengjie verfasserin aut Zhang, Xiu-mei verfasserin aut Xu, Chuan-tian verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 161(2016), 11 vom: 12. Aug., Seite 3081-3093 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:161 year:2016 number:11 day:12 month:08 pages:3081-3093 https://dx.doi.org/10.1007/s00705-016-3013-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 161 2016 11 12 08 3081-3093 |
| allfieldsSound |
10.1007/s00705-016-3013-6 doi (DE-627)SPR007411715 (SPR)s00705-016-3013-6-e DE-627 ger DE-627 rakwb eng 610 ASE 42.32 bkl 44.43 bkl Zhang, Weijun verfasserin aut Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells. Antibodies against NP in pCAGGS-NP-inoculated specific-pathogen-free chickens were detected by enzyme-linked immunosorbent assay (ELISA). Interferon γ (IFN-γ) mRNA was quantified, and IFN-γ production was evaluated using quantitative reverse transcription PCR and capture ELISA, respectively. $ CD8^{+} $ T-lymphocyte proliferation was detected using flow cytometric analysis. The BF2*15 MHC class I binding motif “x-Arg/Lys-x-x-x-Arg/Lys” was explored. Quantification of chicken IFN-γ mRNA, evaluation of IFN-γ production, and measurement of $ CD8^{+} $ T-lymphocyte proliferation confirmed that the peptide $ NP_{67–74} $ of H5N1 was the BF2*15 MHC-class-I-restricted CTL epitope. Major Histocompatibility Complex (dpeaa)DE-He213 Major Histocompatibility Complex Class (dpeaa)DE-He213 Molecular Docking (dpeaa)DE-He213 H5N1 Virus (dpeaa)DE-He213 Infectious Bronchitis Virus (dpeaa)DE-He213 Huang, Qinghua verfasserin aut Lu, Mei verfasserin aut Zhu, Fengzhu verfasserin aut Huang, Yan-yan verfasserin aut Yang, Shao-hua verfasserin aut Kong, Zhengjie verfasserin aut Zhang, Xiu-mei verfasserin aut Xu, Chuan-tian verfasserin aut Enthalten in Archives of virology Wien : Springer, 1939 161(2016), 11 vom: 12. Aug., Seite 3081-3093 (DE-627)253390168 (DE-600)1458460-8 1432-8798 nnns volume:161 year:2016 number:11 day:12 month:08 pages:3081-3093 https://dx.doi.org/10.1007/s00705-016-3013-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.32 ASE 44.43 ASE AR 161 2016 11 12 08 3081-3093 |
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Enthalten in Archives of virology 161(2016), 11 vom: 12. Aug., Seite 3081-3093 volume:161 year:2016 number:11 day:12 month:08 pages:3081-3093 |
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Enthalten in Archives of virology 161(2016), 11 vom: 12. Aug., Seite 3081-3093 volume:161 year:2016 number:11 day:12 month:08 pages:3081-3093 |
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Major Histocompatibility Complex Major Histocompatibility Complex Class Molecular Docking H5N1 Virus Infectious Bronchitis Virus |
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Zhang, Weijun @@aut@@ Huang, Qinghua @@aut@@ Lu, Mei @@aut@@ Zhu, Fengzhu @@aut@@ Huang, Yan-yan @@aut@@ Yang, Shao-hua @@aut@@ Kong, Zhengjie @@aut@@ Zhang, Xiu-mei @@aut@@ Xu, Chuan-tian @@aut@@ |
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2016-08-12T00:00:00Z |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR007411715</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519193617.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201005s2016 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00705-016-3013-6</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR007411715</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00705-016-3013-6-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.32</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.43</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Zhang, Weijun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells. Antibodies against NP in pCAGGS-NP-inoculated specific-pathogen-free chickens were detected by enzyme-linked immunosorbent assay (ELISA). Interferon γ (IFN-γ) mRNA was quantified, and IFN-γ production was evaluated using quantitative reverse transcription PCR and capture ELISA, respectively. $ CD8^{+} $ T-lymphocyte proliferation was detected using flow cytometric analysis. The BF2*15 MHC class I binding motif “x-Arg/Lys-x-x-x-Arg/Lys” was explored. Quantification of chicken IFN-γ mRNA, evaluation of IFN-γ production, and measurement of $ CD8^{+} $ T-lymphocyte proliferation confirmed that the peptide $ NP_{67–74} $ of H5N1 was the BF2*15 MHC-class-I-restricted CTL epitope.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Major Histocompatibility Complex</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Major Histocompatibility Complex Class</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Molecular Docking</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">H5N1 Virus</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Infectious Bronchitis Virus</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Huang, Qinghua</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Mei</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhu, Fengzhu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Huang, Yan-yan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Shao-hua</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kong, Zhengjie</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Xiu-mei</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Chuan-tian</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Archives of virology</subfield><subfield code="d">Wien : Springer, 1939</subfield><subfield code="g">161(2016), 11 vom: 12. 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|
| author |
Zhang, Weijun |
| spellingShingle |
Zhang, Weijun ddc 610 bkl 42.32 bkl 44.43 misc Major Histocompatibility Complex misc Major Histocompatibility Complex Class misc Molecular Docking misc H5N1 Virus misc Infectious Bronchitis Virus Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens |
| authorStr |
Zhang, Weijun |
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@@773@@(DE-627)253390168 |
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electronic Article |
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610 - Medicine & health |
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keep |
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aut aut aut aut aut aut aut aut aut |
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springer |
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true |
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Not Illustrated |
| issn |
1432-8798 |
| topic_title |
610 ASE 42.32 bkl 44.43 bkl Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens Major Histocompatibility Complex (dpeaa)DE-He213 Major Histocompatibility Complex Class (dpeaa)DE-He213 Molecular Docking (dpeaa)DE-He213 H5N1 Virus (dpeaa)DE-He213 Infectious Bronchitis Virus (dpeaa)DE-He213 |
| topic |
ddc 610 bkl 42.32 bkl 44.43 misc Major Histocompatibility Complex misc Major Histocompatibility Complex Class misc Molecular Docking misc H5N1 Virus misc Infectious Bronchitis Virus |
| topic_unstemmed |
ddc 610 bkl 42.32 bkl 44.43 misc Major Histocompatibility Complex misc Major Histocompatibility Complex Class misc Molecular Docking misc H5N1 Virus misc Infectious Bronchitis Virus |
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ddc 610 bkl 42.32 bkl 44.43 misc Major Histocompatibility Complex misc Major Histocompatibility Complex Class misc Molecular Docking misc H5N1 Virus misc Infectious Bronchitis Virus |
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Elektronische Aufsätze Aufsätze Elektronische Ressource |
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Text Zeitschrift/Artikel |
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cr |
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Archives of virology |
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253390168 |
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Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens |
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Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens |
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Zhang, Weijun |
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Archives of virology |
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Zhang, Weijun Huang, Qinghua Lu, Mei Zhu, Fengzhu Huang, Yan-yan Yang, Shao-hua Kong, Zhengjie Zhang, Xiu-mei Xu, Chuan-tian |
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610 |
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exploration of the bf2*15 major histocompatibility complex class i binding motif and identification of cytotoxic t lymphocyte epitopes from the h5n1 influenza virus nucleoprotein in chickens |
| title_auth |
Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens |
| abstract |
Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells. Antibodies against NP in pCAGGS-NP-inoculated specific-pathogen-free chickens were detected by enzyme-linked immunosorbent assay (ELISA). Interferon γ (IFN-γ) mRNA was quantified, and IFN-γ production was evaluated using quantitative reverse transcription PCR and capture ELISA, respectively. $ CD8^{+} $ T-lymphocyte proliferation was detected using flow cytometric analysis. The BF2*15 MHC class I binding motif “x-Arg/Lys-x-x-x-Arg/Lys” was explored. Quantification of chicken IFN-γ mRNA, evaluation of IFN-γ production, and measurement of $ CD8^{+} $ T-lymphocyte proliferation confirmed that the peptide $ NP_{67–74} $ of H5N1 was the BF2*15 MHC-class-I-restricted CTL epitope. |
| abstractGer |
Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells. Antibodies against NP in pCAGGS-NP-inoculated specific-pathogen-free chickens were detected by enzyme-linked immunosorbent assay (ELISA). Interferon γ (IFN-γ) mRNA was quantified, and IFN-γ production was evaluated using quantitative reverse transcription PCR and capture ELISA, respectively. $ CD8^{+} $ T-lymphocyte proliferation was detected using flow cytometric analysis. The BF2*15 MHC class I binding motif “x-Arg/Lys-x-x-x-Arg/Lys” was explored. Quantification of chicken IFN-γ mRNA, evaluation of IFN-γ production, and measurement of $ CD8^{+} $ T-lymphocyte proliferation confirmed that the peptide $ NP_{67–74} $ of H5N1 was the BF2*15 MHC-class-I-restricted CTL epitope. |
| abstract_unstemmed |
Abstract The binding motif of BF2*15 major histocompatibility complex (MHC) class I was explored by analyzing the interaction between an infectious bronchitis virus octapeptide and BF2*15, and the cytotoxic T lymphocyte (CTL) epitope from the nucleoprotein (NP) of H5N1 virus was identified using experimental methods. Computational methods, including homology modeling, molecular dynamics simulation, and molecular docking analysis, were used. The recombinant plasmid pCAGGS-NP was constructed, and NP expression was confirmed by indirect immunofluorescence and Western blot in transfected 293T cells. Antibodies against NP in pCAGGS-NP-inoculated specific-pathogen-free chickens were detected by enzyme-linked immunosorbent assay (ELISA). Interferon γ (IFN-γ) mRNA was quantified, and IFN-γ production was evaluated using quantitative reverse transcription PCR and capture ELISA, respectively. $ CD8^{+} $ T-lymphocyte proliferation was detected using flow cytometric analysis. The BF2*15 MHC class I binding motif “x-Arg/Lys-x-x-x-Arg/Lys” was explored. Quantification of chicken IFN-γ mRNA, evaluation of IFN-γ production, and measurement of $ CD8^{+} $ T-lymphocyte proliferation confirmed that the peptide $ NP_{67–74} $ of H5N1 was the BF2*15 MHC-class-I-restricted CTL epitope. |
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Exploration of the BF2*15 major histocompatibility complex class I binding motif and identification of cytotoxic T lymphocyte epitopes from the H5N1 influenza virus nucleoprotein in chickens |
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Huang, Qinghua Lu, Mei Zhu, Fengzhu Huang, Yan-yan Yang, Shao-hua Kong, Zhengjie Zhang, Xiu-mei Xu, Chuan-tian |
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| score |
7.3967133 |