Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions
Abstract We established a system to separately analyze the role of protons and bicarbonate ions in vitro in which the pH of the medium was controlled by HEPES at various concentrations of sodium bicarbonate ($ NaHCO_{3} $) in the absence of carbon dioxide ($ CO_{2} $). Using this system, we demonstr...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kato, Kohtaro [verfasserIn] Matsushita, Misao [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of bone and mineral metabolism - Tokyo : Springer, 1995, 32(2013), 1 vom: 16. Juli, Seite 17-28 |
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| Übergeordnetes Werk: |
volume:32 ; year:2013 ; number:1 ; day:16 ; month:07 ; pages:17-28 |
| Links: |
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| DOI / URN: |
10.1007/s00774-013-0462-9 |
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| Katalog-ID: |
SPR007724713 |
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| 245 | 1 | 0 | |a Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions |
| 264 | 1 | |c 2013 | |
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| 520 | |a Abstract We established a system to separately analyze the role of protons and bicarbonate ions in vitro in which the pH of the medium was controlled by HEPES at various concentrations of sodium bicarbonate ($ NaHCO_{3} $) in the absence of carbon dioxide ($ CO_{2} $). Using this system, we demonstrated that acidosis promoted osteoclast formation independently of extracellular $ NaHCO_{3} $ in a short-term culture. Protons and bicarbonate ions acted on osteoclast differentiation with opposite effects, the former positively and the latter negatively. The HEPES-based system maintained pH in the absence of extracellular $ NaHCO_{3} $ without $ CO_{2} $. Therefore, we could demonstrate that osteoblast differentiation was promoted at higher pH in a long-term culture system without $ NaHCO_{3} $ in which ALP activity and nodule mineralization were enhanced. This finding indicates that protons negatively control osteoblast differentiation independently of extracellular bicarbonate ions. However, the difference in the concentration of $ NaHCO_{3} $ did not have any influence on nodule mineralization. The opposite effects of protons, the promotion of osteoclast formation and the inhibition of osteoblast differentiation, were suppressed in the presence of 5 mM N-acetyl cysteine, a reagent activating the scavenging of reactive oxygen species (ROS), implying that ROS act on both systems, the promotion of large osteoclast formation and the deterioration of osteoblast formation under acidosis. | ||
| 650 | 4 | |a Proton |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Bicarbonate ion |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Osteoclast |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Osteoblast |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Reactive oxygen species |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Matsushita, Misao |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of bone and mineral metabolism |d Tokyo : Springer, 1995 |g 32(2013), 1 vom: 16. Juli, Seite 17-28 |w (DE-627)300185405 |w (DE-600)1481600-3 |x 1435-5604 |7 nnns |
| 773 | 1 | 8 | |g volume:32 |g year:2013 |g number:1 |g day:16 |g month:07 |g pages:17-28 |
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10.1007/s00774-013-0462-9 doi (DE-627)SPR007724713 (SPR)s00774-013-0462-9-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE 42.17 bkl 42.15 bkl Kato, Kohtaro verfasserin aut Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We established a system to separately analyze the role of protons and bicarbonate ions in vitro in which the pH of the medium was controlled by HEPES at various concentrations of sodium bicarbonate ($ NaHCO_{3} $) in the absence of carbon dioxide ($ CO_{2} $). Using this system, we demonstrated that acidosis promoted osteoclast formation independently of extracellular $ NaHCO_{3} $ in a short-term culture. Protons and bicarbonate ions acted on osteoclast differentiation with opposite effects, the former positively and the latter negatively. The HEPES-based system maintained pH in the absence of extracellular $ NaHCO_{3} $ without $ CO_{2} $. Therefore, we could demonstrate that osteoblast differentiation was promoted at higher pH in a long-term culture system without $ NaHCO_{3} $ in which ALP activity and nodule mineralization were enhanced. This finding indicates that protons negatively control osteoblast differentiation independently of extracellular bicarbonate ions. However, the difference in the concentration of $ NaHCO_{3} $ did not have any influence on nodule mineralization. The opposite effects of protons, the promotion of osteoclast formation and the inhibition of osteoblast differentiation, were suppressed in the presence of 5 mM N-acetyl cysteine, a reagent activating the scavenging of reactive oxygen species (ROS), implying that ROS act on both systems, the promotion of large osteoclast formation and the deterioration of osteoblast formation under acidosis. Proton (dpeaa)DE-He213 Bicarbonate ion (dpeaa)DE-He213 Osteoclast (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Reactive oxygen species (dpeaa)DE-He213 Matsushita, Misao verfasserin aut Enthalten in Journal of bone and mineral metabolism Tokyo : Springer, 1995 32(2013), 1 vom: 16. Juli, Seite 17-28 (DE-627)300185405 (DE-600)1481600-3 1435-5604 nnns volume:32 year:2013 number:1 day:16 month:07 pages:17-28 https://dx.doi.org/10.1007/s00774-013-0462-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.17 ASE 42.15 ASE AR 32 2013 1 16 07 17-28 |
| spelling |
10.1007/s00774-013-0462-9 doi (DE-627)SPR007724713 (SPR)s00774-013-0462-9-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE 42.17 bkl 42.15 bkl Kato, Kohtaro verfasserin aut Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We established a system to separately analyze the role of protons and bicarbonate ions in vitro in which the pH of the medium was controlled by HEPES at various concentrations of sodium bicarbonate ($ NaHCO_{3} $) in the absence of carbon dioxide ($ CO_{2} $). Using this system, we demonstrated that acidosis promoted osteoclast formation independently of extracellular $ NaHCO_{3} $ in a short-term culture. Protons and bicarbonate ions acted on osteoclast differentiation with opposite effects, the former positively and the latter negatively. The HEPES-based system maintained pH in the absence of extracellular $ NaHCO_{3} $ without $ CO_{2} $. Therefore, we could demonstrate that osteoblast differentiation was promoted at higher pH in a long-term culture system without $ NaHCO_{3} $ in which ALP activity and nodule mineralization were enhanced. This finding indicates that protons negatively control osteoblast differentiation independently of extracellular bicarbonate ions. However, the difference in the concentration of $ NaHCO_{3} $ did not have any influence on nodule mineralization. The opposite effects of protons, the promotion of osteoclast formation and the inhibition of osteoblast differentiation, were suppressed in the presence of 5 mM N-acetyl cysteine, a reagent activating the scavenging of reactive oxygen species (ROS), implying that ROS act on both systems, the promotion of large osteoclast formation and the deterioration of osteoblast formation under acidosis. Proton (dpeaa)DE-He213 Bicarbonate ion (dpeaa)DE-He213 Osteoclast (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Reactive oxygen species (dpeaa)DE-He213 Matsushita, Misao verfasserin aut Enthalten in Journal of bone and mineral metabolism Tokyo : Springer, 1995 32(2013), 1 vom: 16. Juli, Seite 17-28 (DE-627)300185405 (DE-600)1481600-3 1435-5604 nnns volume:32 year:2013 number:1 day:16 month:07 pages:17-28 https://dx.doi.org/10.1007/s00774-013-0462-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.17 ASE 42.15 ASE AR 32 2013 1 16 07 17-28 |
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10.1007/s00774-013-0462-9 doi (DE-627)SPR007724713 (SPR)s00774-013-0462-9-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE 42.17 bkl 42.15 bkl Kato, Kohtaro verfasserin aut Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We established a system to separately analyze the role of protons and bicarbonate ions in vitro in which the pH of the medium was controlled by HEPES at various concentrations of sodium bicarbonate ($ NaHCO_{3} $) in the absence of carbon dioxide ($ CO_{2} $). Using this system, we demonstrated that acidosis promoted osteoclast formation independently of extracellular $ NaHCO_{3} $ in a short-term culture. Protons and bicarbonate ions acted on osteoclast differentiation with opposite effects, the former positively and the latter negatively. The HEPES-based system maintained pH in the absence of extracellular $ NaHCO_{3} $ without $ CO_{2} $. Therefore, we could demonstrate that osteoblast differentiation was promoted at higher pH in a long-term culture system without $ NaHCO_{3} $ in which ALP activity and nodule mineralization were enhanced. This finding indicates that protons negatively control osteoblast differentiation independently of extracellular bicarbonate ions. However, the difference in the concentration of $ NaHCO_{3} $ did not have any influence on nodule mineralization. The opposite effects of protons, the promotion of osteoclast formation and the inhibition of osteoblast differentiation, were suppressed in the presence of 5 mM N-acetyl cysteine, a reagent activating the scavenging of reactive oxygen species (ROS), implying that ROS act on both systems, the promotion of large osteoclast formation and the deterioration of osteoblast formation under acidosis. Proton (dpeaa)DE-He213 Bicarbonate ion (dpeaa)DE-He213 Osteoclast (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Reactive oxygen species (dpeaa)DE-He213 Matsushita, Misao verfasserin aut Enthalten in Journal of bone and mineral metabolism Tokyo : Springer, 1995 32(2013), 1 vom: 16. Juli, Seite 17-28 (DE-627)300185405 (DE-600)1481600-3 1435-5604 nnns volume:32 year:2013 number:1 day:16 month:07 pages:17-28 https://dx.doi.org/10.1007/s00774-013-0462-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.17 ASE 42.15 ASE AR 32 2013 1 16 07 17-28 |
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10.1007/s00774-013-0462-9 doi (DE-627)SPR007724713 (SPR)s00774-013-0462-9-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE 42.17 bkl 42.15 bkl Kato, Kohtaro verfasserin aut Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We established a system to separately analyze the role of protons and bicarbonate ions in vitro in which the pH of the medium was controlled by HEPES at various concentrations of sodium bicarbonate ($ NaHCO_{3} $) in the absence of carbon dioxide ($ CO_{2} $). Using this system, we demonstrated that acidosis promoted osteoclast formation independently of extracellular $ NaHCO_{3} $ in a short-term culture. Protons and bicarbonate ions acted on osteoclast differentiation with opposite effects, the former positively and the latter negatively. The HEPES-based system maintained pH in the absence of extracellular $ NaHCO_{3} $ without $ CO_{2} $. Therefore, we could demonstrate that osteoblast differentiation was promoted at higher pH in a long-term culture system without $ NaHCO_{3} $ in which ALP activity and nodule mineralization were enhanced. This finding indicates that protons negatively control osteoblast differentiation independently of extracellular bicarbonate ions. However, the difference in the concentration of $ NaHCO_{3} $ did not have any influence on nodule mineralization. The opposite effects of protons, the promotion of osteoclast formation and the inhibition of osteoblast differentiation, were suppressed in the presence of 5 mM N-acetyl cysteine, a reagent activating the scavenging of reactive oxygen species (ROS), implying that ROS act on both systems, the promotion of large osteoclast formation and the deterioration of osteoblast formation under acidosis. Proton (dpeaa)DE-He213 Bicarbonate ion (dpeaa)DE-He213 Osteoclast (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Reactive oxygen species (dpeaa)DE-He213 Matsushita, Misao verfasserin aut Enthalten in Journal of bone and mineral metabolism Tokyo : Springer, 1995 32(2013), 1 vom: 16. Juli, Seite 17-28 (DE-627)300185405 (DE-600)1481600-3 1435-5604 nnns volume:32 year:2013 number:1 day:16 month:07 pages:17-28 https://dx.doi.org/10.1007/s00774-013-0462-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.17 ASE 42.15 ASE AR 32 2013 1 16 07 17-28 |
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10.1007/s00774-013-0462-9 doi (DE-627)SPR007724713 (SPR)s00774-013-0462-9-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE 42.17 bkl 42.15 bkl Kato, Kohtaro verfasserin aut Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We established a system to separately analyze the role of protons and bicarbonate ions in vitro in which the pH of the medium was controlled by HEPES at various concentrations of sodium bicarbonate ($ NaHCO_{3} $) in the absence of carbon dioxide ($ CO_{2} $). Using this system, we demonstrated that acidosis promoted osteoclast formation independently of extracellular $ NaHCO_{3} $ in a short-term culture. Protons and bicarbonate ions acted on osteoclast differentiation with opposite effects, the former positively and the latter negatively. The HEPES-based system maintained pH in the absence of extracellular $ NaHCO_{3} $ without $ CO_{2} $. Therefore, we could demonstrate that osteoblast differentiation was promoted at higher pH in a long-term culture system without $ NaHCO_{3} $ in which ALP activity and nodule mineralization were enhanced. This finding indicates that protons negatively control osteoblast differentiation independently of extracellular bicarbonate ions. However, the difference in the concentration of $ NaHCO_{3} $ did not have any influence on nodule mineralization. The opposite effects of protons, the promotion of osteoclast formation and the inhibition of osteoblast differentiation, were suppressed in the presence of 5 mM N-acetyl cysteine, a reagent activating the scavenging of reactive oxygen species (ROS), implying that ROS act on both systems, the promotion of large osteoclast formation and the deterioration of osteoblast formation under acidosis. Proton (dpeaa)DE-He213 Bicarbonate ion (dpeaa)DE-He213 Osteoclast (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Reactive oxygen species (dpeaa)DE-He213 Matsushita, Misao verfasserin aut Enthalten in Journal of bone and mineral metabolism Tokyo : Springer, 1995 32(2013), 1 vom: 16. Juli, Seite 17-28 (DE-627)300185405 (DE-600)1481600-3 1435-5604 nnns volume:32 year:2013 number:1 day:16 month:07 pages:17-28 https://dx.doi.org/10.1007/s00774-013-0462-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.17 ASE 42.15 ASE AR 32 2013 1 16 07 17-28 |
| language |
English |
| source |
Enthalten in Journal of bone and mineral metabolism 32(2013), 1 vom: 16. Juli, Seite 17-28 volume:32 year:2013 number:1 day:16 month:07 pages:17-28 |
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Enthalten in Journal of bone and mineral metabolism 32(2013), 1 vom: 16. Juli, Seite 17-28 volume:32 year:2013 number:1 day:16 month:07 pages:17-28 |
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Article |
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findex.gbv.de |
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Proton Bicarbonate ion Osteoclast Osteoblast Reactive oxygen species |
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610 |
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false |
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Journal of bone and mineral metabolism |
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Kato, Kohtaro @@aut@@ Matsushita, Misao @@aut@@ |
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2013-07-16T00:00:00Z |
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300185405 |
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Using this system, we demonstrated that acidosis promoted osteoclast formation independently of extracellular $ NaHCO_{3} $ in a short-term culture. Protons and bicarbonate ions acted on osteoclast differentiation with opposite effects, the former positively and the latter negatively. The HEPES-based system maintained pH in the absence of extracellular $ NaHCO_{3} $ without $ CO_{2} $. Therefore, we could demonstrate that osteoblast differentiation was promoted at higher pH in a long-term culture system without $ NaHCO_{3} $ in which ALP activity and nodule mineralization were enhanced. This finding indicates that protons negatively control osteoblast differentiation independently of extracellular bicarbonate ions. However, the difference in the concentration of $ NaHCO_{3} $ did not have any influence on nodule mineralization. The opposite effects of protons, the promotion of osteoclast formation and the inhibition of osteoblast differentiation, were suppressed in the presence of 5 mM N-acetyl cysteine, a reagent activating the scavenging of reactive oxygen species (ROS), implying that ROS act on both systems, the promotion of large osteoclast formation and the deterioration of osteoblast formation under acidosis.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Proton</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bicarbonate ion</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Osteoclast</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Osteoblast</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Reactive oxygen species</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Matsushita, Misao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of bone and mineral metabolism</subfield><subfield code="d">Tokyo : Springer, 1995</subfield><subfield code="g">32(2013), 1 vom: 16. 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Kato, Kohtaro |
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Kato, Kohtaro ddc 610 bkl 42.17 bkl 42.15 misc Proton misc Bicarbonate ion misc Osteoclast misc Osteoblast misc Reactive oxygen species Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions |
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610 ASE 42.17 bkl 42.15 bkl Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions Proton (dpeaa)DE-He213 Bicarbonate ion (dpeaa)DE-He213 Osteoclast (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Reactive oxygen species (dpeaa)DE-He213 |
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ddc 610 bkl 42.17 bkl 42.15 misc Proton misc Bicarbonate ion misc Osteoclast misc Osteoblast misc Reactive oxygen species |
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Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions |
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Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions |
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proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions |
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Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions |
| abstract |
Abstract We established a system to separately analyze the role of protons and bicarbonate ions in vitro in which the pH of the medium was controlled by HEPES at various concentrations of sodium bicarbonate ($ NaHCO_{3} $) in the absence of carbon dioxide ($ CO_{2} $). Using this system, we demonstrated that acidosis promoted osteoclast formation independently of extracellular $ NaHCO_{3} $ in a short-term culture. Protons and bicarbonate ions acted on osteoclast differentiation with opposite effects, the former positively and the latter negatively. The HEPES-based system maintained pH in the absence of extracellular $ NaHCO_{3} $ without $ CO_{2} $. Therefore, we could demonstrate that osteoblast differentiation was promoted at higher pH in a long-term culture system without $ NaHCO_{3} $ in which ALP activity and nodule mineralization were enhanced. This finding indicates that protons negatively control osteoblast differentiation independently of extracellular bicarbonate ions. However, the difference in the concentration of $ NaHCO_{3} $ did not have any influence on nodule mineralization. The opposite effects of protons, the promotion of osteoclast formation and the inhibition of osteoblast differentiation, were suppressed in the presence of 5 mM N-acetyl cysteine, a reagent activating the scavenging of reactive oxygen species (ROS), implying that ROS act on both systems, the promotion of large osteoclast formation and the deterioration of osteoblast formation under acidosis. |
| abstractGer |
Abstract We established a system to separately analyze the role of protons and bicarbonate ions in vitro in which the pH of the medium was controlled by HEPES at various concentrations of sodium bicarbonate ($ NaHCO_{3} $) in the absence of carbon dioxide ($ CO_{2} $). Using this system, we demonstrated that acidosis promoted osteoclast formation independently of extracellular $ NaHCO_{3} $ in a short-term culture. Protons and bicarbonate ions acted on osteoclast differentiation with opposite effects, the former positively and the latter negatively. The HEPES-based system maintained pH in the absence of extracellular $ NaHCO_{3} $ without $ CO_{2} $. Therefore, we could demonstrate that osteoblast differentiation was promoted at higher pH in a long-term culture system without $ NaHCO_{3} $ in which ALP activity and nodule mineralization were enhanced. This finding indicates that protons negatively control osteoblast differentiation independently of extracellular bicarbonate ions. However, the difference in the concentration of $ NaHCO_{3} $ did not have any influence on nodule mineralization. The opposite effects of protons, the promotion of osteoclast formation and the inhibition of osteoblast differentiation, were suppressed in the presence of 5 mM N-acetyl cysteine, a reagent activating the scavenging of reactive oxygen species (ROS), implying that ROS act on both systems, the promotion of large osteoclast formation and the deterioration of osteoblast formation under acidosis. |
| abstract_unstemmed |
Abstract We established a system to separately analyze the role of protons and bicarbonate ions in vitro in which the pH of the medium was controlled by HEPES at various concentrations of sodium bicarbonate ($ NaHCO_{3} $) in the absence of carbon dioxide ($ CO_{2} $). Using this system, we demonstrated that acidosis promoted osteoclast formation independently of extracellular $ NaHCO_{3} $ in a short-term culture. Protons and bicarbonate ions acted on osteoclast differentiation with opposite effects, the former positively and the latter negatively. The HEPES-based system maintained pH in the absence of extracellular $ NaHCO_{3} $ without $ CO_{2} $. Therefore, we could demonstrate that osteoblast differentiation was promoted at higher pH in a long-term culture system without $ NaHCO_{3} $ in which ALP activity and nodule mineralization were enhanced. This finding indicates that protons negatively control osteoblast differentiation independently of extracellular bicarbonate ions. However, the difference in the concentration of $ NaHCO_{3} $ did not have any influence on nodule mineralization. The opposite effects of protons, the promotion of osteoclast formation and the inhibition of osteoblast differentiation, were suppressed in the presence of 5 mM N-acetyl cysteine, a reagent activating the scavenging of reactive oxygen species (ROS), implying that ROS act on both systems, the promotion of large osteoclast formation and the deterioration of osteoblast formation under acidosis. |
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| container_issue |
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| title_short |
Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions |
| url |
https://dx.doi.org/10.1007/s00774-013-0462-9 |
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| author2 |
Matsushita, Misao |
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| doi_str |
10.1007/s00774-013-0462-9 |
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2024-07-03T14:48:23.183Z |
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| score |
7.3982754 |