Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells
Objective This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells. Materials and metho...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Römer, Piero [verfasserIn] Köstler, Josef [verfasserIn] Koretsi, Vasiliki [verfasserIn] Proff, Peter [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Clinical Oral Investigations - Springer-Verlag, 2001, 17(2013), 9 vom: 08. Feb., Seite 2041-2048 |
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| Übergeordnetes Werk: |
volume:17 ; year:2013 ; number:9 ; day:08 ; month:02 ; pages:2041-2048 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00784-013-0928-0 |
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| Katalog-ID: |
SPR007806272 |
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| 520 | |a Objective This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells. Materials and methods In comparison to a control group, three experimental groups were formed from human primary PDL cells stressed with compressive forces, bacterial endotoxins, or a combination of both. Gene expression of cyclooxygenase-2 and RANKL was analysed with RT real-time PCR. The prostaglandin E2 production was determined with ELISA. A co-culture of PDL cells and an osteoclast-progenitor cell line was used in order to demonstrate the osteoclast formation effect caused by the simultaneous combined stress. Results The simultaneous combined stress resulted in a 56-fold up-regulation of cyclooxygenase-2 gene expression with a subsequent noticeable rise in the prostaglandin E2 in the culture medium. The RANKL/osteoprotegerin gene expression ratio was 50-fold up-regulated and the osteoclast formation assay revealed 153.5 ± 15.7 tartrate-resistant acid phosphatase (TRAP)-positive cells per well compared with 42.3 ± 3.8 TRAP-positive cells per well of the control group. Conclusion The synergistic action of periodontal pathogens and orthodontic forces leads to an increased expression of cyclooxygenase-2 from PDL cells that intensify the RANKL production which in turn induces osteoclast differentiation and subsequent osteoclastogenesis. Clinical relevance The present study puts an emphasis on the detrimental effect of orthodontic forces on patients with an active periodontal disease by underlining the significance of cyclooxygenase-2 activity and RANKL binding on the osteoclastogenesis process. | ||
| 650 | 4 | |a Periodontitis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a PDL cells |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Orthodontic tooth movement |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a RANKL |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a COX-2 |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Koretsi, Vasiliki |e verfasserin |4 aut | |
| 700 | 1 | |a Proff, Peter |e verfasserin |4 aut | |
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10.1007/s00784-013-0928-0 doi (DE-627)SPR007806272 (SPR)s00784-013-0928-0-e DE-627 ger DE-627 rakwb eng Römer, Piero verfasserin aut Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells. Materials and methods In comparison to a control group, three experimental groups were formed from human primary PDL cells stressed with compressive forces, bacterial endotoxins, or a combination of both. Gene expression of cyclooxygenase-2 and RANKL was analysed with RT real-time PCR. The prostaglandin E2 production was determined with ELISA. A co-culture of PDL cells and an osteoclast-progenitor cell line was used in order to demonstrate the osteoclast formation effect caused by the simultaneous combined stress. Results The simultaneous combined stress resulted in a 56-fold up-regulation of cyclooxygenase-2 gene expression with a subsequent noticeable rise in the prostaglandin E2 in the culture medium. The RANKL/osteoprotegerin gene expression ratio was 50-fold up-regulated and the osteoclast formation assay revealed 153.5 ± 15.7 tartrate-resistant acid phosphatase (TRAP)-positive cells per well compared with 42.3 ± 3.8 TRAP-positive cells per well of the control group. Conclusion The synergistic action of periodontal pathogens and orthodontic forces leads to an increased expression of cyclooxygenase-2 from PDL cells that intensify the RANKL production which in turn induces osteoclast differentiation and subsequent osteoclastogenesis. Clinical relevance The present study puts an emphasis on the detrimental effect of orthodontic forces on patients with an active periodontal disease by underlining the significance of cyclooxygenase-2 activity and RANKL binding on the osteoclastogenesis process. Periodontitis (dpeaa)DE-He213 PDL cells (dpeaa)DE-He213 Orthodontic tooth movement (dpeaa)DE-He213 RANKL (dpeaa)DE-He213 COX-2 (dpeaa)DE-He213 OPG (dpeaa)DE-He213 Köstler, Josef verfasserin aut Koretsi, Vasiliki verfasserin aut Proff, Peter verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 17(2013), 9 vom: 08. Feb., Seite 2041-2048 (DE-627)SPR007794231 nnns volume:17 year:2013 number:9 day:08 month:02 pages:2041-2048 https://dx.doi.org/10.1007/s00784-013-0928-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 17 2013 9 08 02 2041-2048 |
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10.1007/s00784-013-0928-0 doi (DE-627)SPR007806272 (SPR)s00784-013-0928-0-e DE-627 ger DE-627 rakwb eng Römer, Piero verfasserin aut Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells. Materials and methods In comparison to a control group, three experimental groups were formed from human primary PDL cells stressed with compressive forces, bacterial endotoxins, or a combination of both. Gene expression of cyclooxygenase-2 and RANKL was analysed with RT real-time PCR. The prostaglandin E2 production was determined with ELISA. A co-culture of PDL cells and an osteoclast-progenitor cell line was used in order to demonstrate the osteoclast formation effect caused by the simultaneous combined stress. Results The simultaneous combined stress resulted in a 56-fold up-regulation of cyclooxygenase-2 gene expression with a subsequent noticeable rise in the prostaglandin E2 in the culture medium. The RANKL/osteoprotegerin gene expression ratio was 50-fold up-regulated and the osteoclast formation assay revealed 153.5 ± 15.7 tartrate-resistant acid phosphatase (TRAP)-positive cells per well compared with 42.3 ± 3.8 TRAP-positive cells per well of the control group. Conclusion The synergistic action of periodontal pathogens and orthodontic forces leads to an increased expression of cyclooxygenase-2 from PDL cells that intensify the RANKL production which in turn induces osteoclast differentiation and subsequent osteoclastogenesis. Clinical relevance The present study puts an emphasis on the detrimental effect of orthodontic forces on patients with an active periodontal disease by underlining the significance of cyclooxygenase-2 activity and RANKL binding on the osteoclastogenesis process. Periodontitis (dpeaa)DE-He213 PDL cells (dpeaa)DE-He213 Orthodontic tooth movement (dpeaa)DE-He213 RANKL (dpeaa)DE-He213 COX-2 (dpeaa)DE-He213 OPG (dpeaa)DE-He213 Köstler, Josef verfasserin aut Koretsi, Vasiliki verfasserin aut Proff, Peter verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 17(2013), 9 vom: 08. Feb., Seite 2041-2048 (DE-627)SPR007794231 nnns volume:17 year:2013 number:9 day:08 month:02 pages:2041-2048 https://dx.doi.org/10.1007/s00784-013-0928-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 17 2013 9 08 02 2041-2048 |
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10.1007/s00784-013-0928-0 doi (DE-627)SPR007806272 (SPR)s00784-013-0928-0-e DE-627 ger DE-627 rakwb eng Römer, Piero verfasserin aut Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells. Materials and methods In comparison to a control group, three experimental groups were formed from human primary PDL cells stressed with compressive forces, bacterial endotoxins, or a combination of both. Gene expression of cyclooxygenase-2 and RANKL was analysed with RT real-time PCR. The prostaglandin E2 production was determined with ELISA. A co-culture of PDL cells and an osteoclast-progenitor cell line was used in order to demonstrate the osteoclast formation effect caused by the simultaneous combined stress. Results The simultaneous combined stress resulted in a 56-fold up-regulation of cyclooxygenase-2 gene expression with a subsequent noticeable rise in the prostaglandin E2 in the culture medium. The RANKL/osteoprotegerin gene expression ratio was 50-fold up-regulated and the osteoclast formation assay revealed 153.5 ± 15.7 tartrate-resistant acid phosphatase (TRAP)-positive cells per well compared with 42.3 ± 3.8 TRAP-positive cells per well of the control group. Conclusion The synergistic action of periodontal pathogens and orthodontic forces leads to an increased expression of cyclooxygenase-2 from PDL cells that intensify the RANKL production which in turn induces osteoclast differentiation and subsequent osteoclastogenesis. Clinical relevance The present study puts an emphasis on the detrimental effect of orthodontic forces on patients with an active periodontal disease by underlining the significance of cyclooxygenase-2 activity and RANKL binding on the osteoclastogenesis process. Periodontitis (dpeaa)DE-He213 PDL cells (dpeaa)DE-He213 Orthodontic tooth movement (dpeaa)DE-He213 RANKL (dpeaa)DE-He213 COX-2 (dpeaa)DE-He213 OPG (dpeaa)DE-He213 Köstler, Josef verfasserin aut Koretsi, Vasiliki verfasserin aut Proff, Peter verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 17(2013), 9 vom: 08. Feb., Seite 2041-2048 (DE-627)SPR007794231 nnns volume:17 year:2013 number:9 day:08 month:02 pages:2041-2048 https://dx.doi.org/10.1007/s00784-013-0928-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 17 2013 9 08 02 2041-2048 |
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10.1007/s00784-013-0928-0 doi (DE-627)SPR007806272 (SPR)s00784-013-0928-0-e DE-627 ger DE-627 rakwb eng Römer, Piero verfasserin aut Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells. Materials and methods In comparison to a control group, three experimental groups were formed from human primary PDL cells stressed with compressive forces, bacterial endotoxins, or a combination of both. Gene expression of cyclooxygenase-2 and RANKL was analysed with RT real-time PCR. The prostaglandin E2 production was determined with ELISA. A co-culture of PDL cells and an osteoclast-progenitor cell line was used in order to demonstrate the osteoclast formation effect caused by the simultaneous combined stress. Results The simultaneous combined stress resulted in a 56-fold up-regulation of cyclooxygenase-2 gene expression with a subsequent noticeable rise in the prostaglandin E2 in the culture medium. The RANKL/osteoprotegerin gene expression ratio was 50-fold up-regulated and the osteoclast formation assay revealed 153.5 ± 15.7 tartrate-resistant acid phosphatase (TRAP)-positive cells per well compared with 42.3 ± 3.8 TRAP-positive cells per well of the control group. Conclusion The synergistic action of periodontal pathogens and orthodontic forces leads to an increased expression of cyclooxygenase-2 from PDL cells that intensify the RANKL production which in turn induces osteoclast differentiation and subsequent osteoclastogenesis. Clinical relevance The present study puts an emphasis on the detrimental effect of orthodontic forces on patients with an active periodontal disease by underlining the significance of cyclooxygenase-2 activity and RANKL binding on the osteoclastogenesis process. Periodontitis (dpeaa)DE-He213 PDL cells (dpeaa)DE-He213 Orthodontic tooth movement (dpeaa)DE-He213 RANKL (dpeaa)DE-He213 COX-2 (dpeaa)DE-He213 OPG (dpeaa)DE-He213 Köstler, Josef verfasserin aut Koretsi, Vasiliki verfasserin aut Proff, Peter verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 17(2013), 9 vom: 08. Feb., Seite 2041-2048 (DE-627)SPR007794231 nnns volume:17 year:2013 number:9 day:08 month:02 pages:2041-2048 https://dx.doi.org/10.1007/s00784-013-0928-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 17 2013 9 08 02 2041-2048 |
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10.1007/s00784-013-0928-0 doi (DE-627)SPR007806272 (SPR)s00784-013-0928-0-e DE-627 ger DE-627 rakwb eng Römer, Piero verfasserin aut Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells. Materials and methods In comparison to a control group, three experimental groups were formed from human primary PDL cells stressed with compressive forces, bacterial endotoxins, or a combination of both. Gene expression of cyclooxygenase-2 and RANKL was analysed with RT real-time PCR. The prostaglandin E2 production was determined with ELISA. A co-culture of PDL cells and an osteoclast-progenitor cell line was used in order to demonstrate the osteoclast formation effect caused by the simultaneous combined stress. Results The simultaneous combined stress resulted in a 56-fold up-regulation of cyclooxygenase-2 gene expression with a subsequent noticeable rise in the prostaglandin E2 in the culture medium. The RANKL/osteoprotegerin gene expression ratio was 50-fold up-regulated and the osteoclast formation assay revealed 153.5 ± 15.7 tartrate-resistant acid phosphatase (TRAP)-positive cells per well compared with 42.3 ± 3.8 TRAP-positive cells per well of the control group. Conclusion The synergistic action of periodontal pathogens and orthodontic forces leads to an increased expression of cyclooxygenase-2 from PDL cells that intensify the RANKL production which in turn induces osteoclast differentiation and subsequent osteoclastogenesis. Clinical relevance The present study puts an emphasis on the detrimental effect of orthodontic forces on patients with an active periodontal disease by underlining the significance of cyclooxygenase-2 activity and RANKL binding on the osteoclastogenesis process. Periodontitis (dpeaa)DE-He213 PDL cells (dpeaa)DE-He213 Orthodontic tooth movement (dpeaa)DE-He213 RANKL (dpeaa)DE-He213 COX-2 (dpeaa)DE-He213 OPG (dpeaa)DE-He213 Köstler, Josef verfasserin aut Koretsi, Vasiliki verfasserin aut Proff, Peter verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 17(2013), 9 vom: 08. Feb., Seite 2041-2048 (DE-627)SPR007794231 nnns volume:17 year:2013 number:9 day:08 month:02 pages:2041-2048 https://dx.doi.org/10.1007/s00784-013-0928-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 17 2013 9 08 02 2041-2048 |
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Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells |
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Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells |
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Römer, Piero |
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Clinical Oral Investigations |
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Clinical Oral Investigations |
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eng |
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2013 |
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2041 |
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Römer, Piero Köstler, Josef Koretsi, Vasiliki Proff, Peter |
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Elektronische Aufsätze |
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Römer, Piero |
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10.1007/s00784-013-0928-0 |
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verfasserin |
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endotoxins potentiate cox-2 and rankl expression in compressed pdl cells |
| title_auth |
Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells |
| abstract |
Objective This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells. Materials and methods In comparison to a control group, three experimental groups were formed from human primary PDL cells stressed with compressive forces, bacterial endotoxins, or a combination of both. Gene expression of cyclooxygenase-2 and RANKL was analysed with RT real-time PCR. The prostaglandin E2 production was determined with ELISA. A co-culture of PDL cells and an osteoclast-progenitor cell line was used in order to demonstrate the osteoclast formation effect caused by the simultaneous combined stress. Results The simultaneous combined stress resulted in a 56-fold up-regulation of cyclooxygenase-2 gene expression with a subsequent noticeable rise in the prostaglandin E2 in the culture medium. The RANKL/osteoprotegerin gene expression ratio was 50-fold up-regulated and the osteoclast formation assay revealed 153.5 ± 15.7 tartrate-resistant acid phosphatase (TRAP)-positive cells per well compared with 42.3 ± 3.8 TRAP-positive cells per well of the control group. Conclusion The synergistic action of periodontal pathogens and orthodontic forces leads to an increased expression of cyclooxygenase-2 from PDL cells that intensify the RANKL production which in turn induces osteoclast differentiation and subsequent osteoclastogenesis. Clinical relevance The present study puts an emphasis on the detrimental effect of orthodontic forces on patients with an active periodontal disease by underlining the significance of cyclooxygenase-2 activity and RANKL binding on the osteoclastogenesis process. |
| abstractGer |
Objective This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells. Materials and methods In comparison to a control group, three experimental groups were formed from human primary PDL cells stressed with compressive forces, bacterial endotoxins, or a combination of both. Gene expression of cyclooxygenase-2 and RANKL was analysed with RT real-time PCR. The prostaglandin E2 production was determined with ELISA. A co-culture of PDL cells and an osteoclast-progenitor cell line was used in order to demonstrate the osteoclast formation effect caused by the simultaneous combined stress. Results The simultaneous combined stress resulted in a 56-fold up-regulation of cyclooxygenase-2 gene expression with a subsequent noticeable rise in the prostaglandin E2 in the culture medium. The RANKL/osteoprotegerin gene expression ratio was 50-fold up-regulated and the osteoclast formation assay revealed 153.5 ± 15.7 tartrate-resistant acid phosphatase (TRAP)-positive cells per well compared with 42.3 ± 3.8 TRAP-positive cells per well of the control group. Conclusion The synergistic action of periodontal pathogens and orthodontic forces leads to an increased expression of cyclooxygenase-2 from PDL cells that intensify the RANKL production which in turn induces osteoclast differentiation and subsequent osteoclastogenesis. Clinical relevance The present study puts an emphasis on the detrimental effect of orthodontic forces on patients with an active periodontal disease by underlining the significance of cyclooxygenase-2 activity and RANKL binding on the osteoclastogenesis process. |
| abstract_unstemmed |
Objective This study aims to demonstrate in vitro the synergistic effect of orthodontic forces and periodontal pathogens on cyclooxygenase-2 regulation and the subsequent receptor activator of nuclear factor kappa-B ligand (RANKL) production from periodontal ligament (PDL) cells. Materials and methods In comparison to a control group, three experimental groups were formed from human primary PDL cells stressed with compressive forces, bacterial endotoxins, or a combination of both. Gene expression of cyclooxygenase-2 and RANKL was analysed with RT real-time PCR. The prostaglandin E2 production was determined with ELISA. A co-culture of PDL cells and an osteoclast-progenitor cell line was used in order to demonstrate the osteoclast formation effect caused by the simultaneous combined stress. Results The simultaneous combined stress resulted in a 56-fold up-regulation of cyclooxygenase-2 gene expression with a subsequent noticeable rise in the prostaglandin E2 in the culture medium. The RANKL/osteoprotegerin gene expression ratio was 50-fold up-regulated and the osteoclast formation assay revealed 153.5 ± 15.7 tartrate-resistant acid phosphatase (TRAP)-positive cells per well compared with 42.3 ± 3.8 TRAP-positive cells per well of the control group. Conclusion The synergistic action of periodontal pathogens and orthodontic forces leads to an increased expression of cyclooxygenase-2 from PDL cells that intensify the RANKL production which in turn induces osteoclast differentiation and subsequent osteoclastogenesis. Clinical relevance The present study puts an emphasis on the detrimental effect of orthodontic forces on patients with an active periodontal disease by underlining the significance of cyclooxygenase-2 activity and RANKL binding on the osteoclastogenesis process. |
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Endotoxins potentiate COX-2 and RANKL expression in compressed PDL cells |
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https://dx.doi.org/10.1007/s00784-013-0928-0 |
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Köstler, Josef Koretsi, Vasiliki Proff, Peter |
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2024-07-03T15:21:39.262Z |
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