Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production
Introduction This study investigated the presence of different Gram-negative bacterial species and the levels of endotoxins found in primary endodontic infection (PEI), determining their stimulation ability against macrophages through the levels of interleukin (IL)-1, IL-6, IL-10, and tumor necrosis...
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| Autor*in: |
Martinho, Frederico C. [verfasserIn] Leite, Fábio R. M. [verfasserIn] Nascimento, Gustavo G. [verfasserIn] Cirelli, Joni A. [verfasserIn] Gomes, Brenda P. F. A. [verfasserIn] |
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Übergeordnetes Werk: |
Enthalten in: Clinical Oral Investigations - Springer-Verlag, 2001, 18(2014), 9 vom: 13. Feb., Seite 2095-2102 |
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| Übergeordnetes Werk: |
volume:18 ; year:2014 ; number:9 ; day:13 ; month:02 ; pages:2095-2102 |
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| DOI / URN: |
10.1007/s00784-014-1198-1 |
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SPR007809247 |
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| 520 | |a Introduction This study investigated the presence of different Gram-negative bacterial species and the levels of endotoxins found in primary endodontic infection (PEI), determining their stimulation ability against macrophages through the levels of interleukin (IL)-1, IL-6, IL-10, and tumor necrosis factor alpha (TNF-α), and evaluated their relationship with clinical and radiographic findings. Material and methods Samples were taken from 21 root canals with primary endodontic infection with apical periodontitis (PEIAP). Molecular techniques were used for bacterial detection. Limulus amebocyte lysate assay was used to measure endotoxins. Pro-inflammatory cytokines were measured by ELISA assay. Results All samples were positive for bacterial DNA (21/21). Prevotella nigrescens (57.2 %) was the most frequent species. Higher levels of endotoxins were found in teeth with pain on palpation and exudation (all p < 0.05). Positive correlations were found between endotoxins and the levels of TNF-α and IL-1β, whereas a negative correlation was found between endotoxin and the amount of IL-10 (p < 0.05). Endotoxin levels were found to be a risk factor for exudation and increased the number of Gram-negative bacterial species for the presence of a larger area of bone destruction (all p < 0.05). Conclusion A wide variety of Gram-negative bacterial species are involved in primary endodontic infection, with participation of different Treponema species. Thus, the levels of endotoxins and the number of Gram-negative bacteria species present in root canals were considered risk factors for the severity of endodontic infection. Clinical relevance The present study revealed that Gram-negative bacterial species and endotoxins play an important role in the development of signs/symptoms and the severity of bone destruction, this knowledge is essential for the establishment of an effective therapy. | ||
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| 700 | 1 | |a Cirelli, Joni A. |e verfasserin |4 aut | |
| 700 | 1 | |a Gomes, Brenda P. F. A. |e verfasserin |4 aut | |
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10.1007/s00784-014-1198-1 doi (DE-627)SPR007809247 (SPR)s00784-014-1198-1-e DE-627 ger DE-627 rakwb eng Martinho, Frederico C. verfasserin aut Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction This study investigated the presence of different Gram-negative bacterial species and the levels of endotoxins found in primary endodontic infection (PEI), determining their stimulation ability against macrophages through the levels of interleukin (IL)-1, IL-6, IL-10, and tumor necrosis factor alpha (TNF-α), and evaluated their relationship with clinical and radiographic findings. Material and methods Samples were taken from 21 root canals with primary endodontic infection with apical periodontitis (PEIAP). Molecular techniques were used for bacterial detection. Limulus amebocyte lysate assay was used to measure endotoxins. Pro-inflammatory cytokines were measured by ELISA assay. Results All samples were positive for bacterial DNA (21/21). Prevotella nigrescens (57.2 %) was the most frequent species. Higher levels of endotoxins were found in teeth with pain on palpation and exudation (all p < 0.05). Positive correlations were found between endotoxins and the levels of TNF-α and IL-1β, whereas a negative correlation was found between endotoxin and the amount of IL-10 (p < 0.05). Endotoxin levels were found to be a risk factor for exudation and increased the number of Gram-negative bacterial species for the presence of a larger area of bone destruction (all p < 0.05). Conclusion A wide variety of Gram-negative bacterial species are involved in primary endodontic infection, with participation of different Treponema species. Thus, the levels of endotoxins and the number of Gram-negative bacteria species present in root canals were considered risk factors for the severity of endodontic infection. Clinical relevance The present study revealed that Gram-negative bacterial species and endotoxins play an important role in the development of signs/symptoms and the severity of bone destruction, this knowledge is essential for the establishment of an effective therapy. Endotoxin (dpeaa)DE-He213 Periapical periodontitis (dpeaa)DE-He213 Leite, Fábio R. M. verfasserin aut Nascimento, Gustavo G. verfasserin aut Cirelli, Joni A. verfasserin aut Gomes, Brenda P. F. A. verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 18(2014), 9 vom: 13. Feb., Seite 2095-2102 (DE-627)SPR007794231 nnns volume:18 year:2014 number:9 day:13 month:02 pages:2095-2102 https://dx.doi.org/10.1007/s00784-014-1198-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 18 2014 9 13 02 2095-2102 |
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10.1007/s00784-014-1198-1 doi (DE-627)SPR007809247 (SPR)s00784-014-1198-1-e DE-627 ger DE-627 rakwb eng Martinho, Frederico C. verfasserin aut Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction This study investigated the presence of different Gram-negative bacterial species and the levels of endotoxins found in primary endodontic infection (PEI), determining their stimulation ability against macrophages through the levels of interleukin (IL)-1, IL-6, IL-10, and tumor necrosis factor alpha (TNF-α), and evaluated their relationship with clinical and radiographic findings. Material and methods Samples were taken from 21 root canals with primary endodontic infection with apical periodontitis (PEIAP). Molecular techniques were used for bacterial detection. Limulus amebocyte lysate assay was used to measure endotoxins. Pro-inflammatory cytokines were measured by ELISA assay. Results All samples were positive for bacterial DNA (21/21). Prevotella nigrescens (57.2 %) was the most frequent species. Higher levels of endotoxins were found in teeth with pain on palpation and exudation (all p < 0.05). Positive correlations were found between endotoxins and the levels of TNF-α and IL-1β, whereas a negative correlation was found between endotoxin and the amount of IL-10 (p < 0.05). Endotoxin levels were found to be a risk factor for exudation and increased the number of Gram-negative bacterial species for the presence of a larger area of bone destruction (all p < 0.05). Conclusion A wide variety of Gram-negative bacterial species are involved in primary endodontic infection, with participation of different Treponema species. Thus, the levels of endotoxins and the number of Gram-negative bacteria species present in root canals were considered risk factors for the severity of endodontic infection. Clinical relevance The present study revealed that Gram-negative bacterial species and endotoxins play an important role in the development of signs/symptoms and the severity of bone destruction, this knowledge is essential for the establishment of an effective therapy. Endotoxin (dpeaa)DE-He213 Periapical periodontitis (dpeaa)DE-He213 Leite, Fábio R. M. verfasserin aut Nascimento, Gustavo G. verfasserin aut Cirelli, Joni A. verfasserin aut Gomes, Brenda P. F. A. verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 18(2014), 9 vom: 13. Feb., Seite 2095-2102 (DE-627)SPR007794231 nnns volume:18 year:2014 number:9 day:13 month:02 pages:2095-2102 https://dx.doi.org/10.1007/s00784-014-1198-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 18 2014 9 13 02 2095-2102 |
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10.1007/s00784-014-1198-1 doi (DE-627)SPR007809247 (SPR)s00784-014-1198-1-e DE-627 ger DE-627 rakwb eng Martinho, Frederico C. verfasserin aut Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction This study investigated the presence of different Gram-negative bacterial species and the levels of endotoxins found in primary endodontic infection (PEI), determining their stimulation ability against macrophages through the levels of interleukin (IL)-1, IL-6, IL-10, and tumor necrosis factor alpha (TNF-α), and evaluated their relationship with clinical and radiographic findings. Material and methods Samples were taken from 21 root canals with primary endodontic infection with apical periodontitis (PEIAP). Molecular techniques were used for bacterial detection. Limulus amebocyte lysate assay was used to measure endotoxins. Pro-inflammatory cytokines were measured by ELISA assay. Results All samples were positive for bacterial DNA (21/21). Prevotella nigrescens (57.2 %) was the most frequent species. Higher levels of endotoxins were found in teeth with pain on palpation and exudation (all p < 0.05). Positive correlations were found between endotoxins and the levels of TNF-α and IL-1β, whereas a negative correlation was found between endotoxin and the amount of IL-10 (p < 0.05). Endotoxin levels were found to be a risk factor for exudation and increased the number of Gram-negative bacterial species for the presence of a larger area of bone destruction (all p < 0.05). Conclusion A wide variety of Gram-negative bacterial species are involved in primary endodontic infection, with participation of different Treponema species. Thus, the levels of endotoxins and the number of Gram-negative bacteria species present in root canals were considered risk factors for the severity of endodontic infection. Clinical relevance The present study revealed that Gram-negative bacterial species and endotoxins play an important role in the development of signs/symptoms and the severity of bone destruction, this knowledge is essential for the establishment of an effective therapy. Endotoxin (dpeaa)DE-He213 Periapical periodontitis (dpeaa)DE-He213 Leite, Fábio R. M. verfasserin aut Nascimento, Gustavo G. verfasserin aut Cirelli, Joni A. verfasserin aut Gomes, Brenda P. F. A. verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 18(2014), 9 vom: 13. Feb., Seite 2095-2102 (DE-627)SPR007794231 nnns volume:18 year:2014 number:9 day:13 month:02 pages:2095-2102 https://dx.doi.org/10.1007/s00784-014-1198-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 18 2014 9 13 02 2095-2102 |
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10.1007/s00784-014-1198-1 doi (DE-627)SPR007809247 (SPR)s00784-014-1198-1-e DE-627 ger DE-627 rakwb eng Martinho, Frederico C. verfasserin aut Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction This study investigated the presence of different Gram-negative bacterial species and the levels of endotoxins found in primary endodontic infection (PEI), determining their stimulation ability against macrophages through the levels of interleukin (IL)-1, IL-6, IL-10, and tumor necrosis factor alpha (TNF-α), and evaluated their relationship with clinical and radiographic findings. Material and methods Samples were taken from 21 root canals with primary endodontic infection with apical periodontitis (PEIAP). Molecular techniques were used for bacterial detection. Limulus amebocyte lysate assay was used to measure endotoxins. Pro-inflammatory cytokines were measured by ELISA assay. Results All samples were positive for bacterial DNA (21/21). Prevotella nigrescens (57.2 %) was the most frequent species. Higher levels of endotoxins were found in teeth with pain on palpation and exudation (all p < 0.05). Positive correlations were found between endotoxins and the levels of TNF-α and IL-1β, whereas a negative correlation was found between endotoxin and the amount of IL-10 (p < 0.05). Endotoxin levels were found to be a risk factor for exudation and increased the number of Gram-negative bacterial species for the presence of a larger area of bone destruction (all p < 0.05). Conclusion A wide variety of Gram-negative bacterial species are involved in primary endodontic infection, with participation of different Treponema species. Thus, the levels of endotoxins and the number of Gram-negative bacteria species present in root canals were considered risk factors for the severity of endodontic infection. Clinical relevance The present study revealed that Gram-negative bacterial species and endotoxins play an important role in the development of signs/symptoms and the severity of bone destruction, this knowledge is essential for the establishment of an effective therapy. Endotoxin (dpeaa)DE-He213 Periapical periodontitis (dpeaa)DE-He213 Leite, Fábio R. M. verfasserin aut Nascimento, Gustavo G. verfasserin aut Cirelli, Joni A. verfasserin aut Gomes, Brenda P. F. A. verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 18(2014), 9 vom: 13. Feb., Seite 2095-2102 (DE-627)SPR007794231 nnns volume:18 year:2014 number:9 day:13 month:02 pages:2095-2102 https://dx.doi.org/10.1007/s00784-014-1198-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 18 2014 9 13 02 2095-2102 |
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10.1007/s00784-014-1198-1 doi (DE-627)SPR007809247 (SPR)s00784-014-1198-1-e DE-627 ger DE-627 rakwb eng Martinho, Frederico C. verfasserin aut Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction This study investigated the presence of different Gram-negative bacterial species and the levels of endotoxins found in primary endodontic infection (PEI), determining their stimulation ability against macrophages through the levels of interleukin (IL)-1, IL-6, IL-10, and tumor necrosis factor alpha (TNF-α), and evaluated their relationship with clinical and radiographic findings. Material and methods Samples were taken from 21 root canals with primary endodontic infection with apical periodontitis (PEIAP). Molecular techniques were used for bacterial detection. Limulus amebocyte lysate assay was used to measure endotoxins. Pro-inflammatory cytokines were measured by ELISA assay. Results All samples were positive for bacterial DNA (21/21). Prevotella nigrescens (57.2 %) was the most frequent species. Higher levels of endotoxins were found in teeth with pain on palpation and exudation (all p < 0.05). Positive correlations were found between endotoxins and the levels of TNF-α and IL-1β, whereas a negative correlation was found between endotoxin and the amount of IL-10 (p < 0.05). Endotoxin levels were found to be a risk factor for exudation and increased the number of Gram-negative bacterial species for the presence of a larger area of bone destruction (all p < 0.05). Conclusion A wide variety of Gram-negative bacterial species are involved in primary endodontic infection, with participation of different Treponema species. Thus, the levels of endotoxins and the number of Gram-negative bacteria species present in root canals were considered risk factors for the severity of endodontic infection. Clinical relevance The present study revealed that Gram-negative bacterial species and endotoxins play an important role in the development of signs/symptoms and the severity of bone destruction, this knowledge is essential for the establishment of an effective therapy. Endotoxin (dpeaa)DE-He213 Periapical periodontitis (dpeaa)DE-He213 Leite, Fábio R. M. verfasserin aut Nascimento, Gustavo G. verfasserin aut Cirelli, Joni A. verfasserin aut Gomes, Brenda P. F. A. verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 18(2014), 9 vom: 13. Feb., Seite 2095-2102 (DE-627)SPR007794231 nnns volume:18 year:2014 number:9 day:13 month:02 pages:2095-2102 https://dx.doi.org/10.1007/s00784-014-1198-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 18 2014 9 13 02 2095-2102 |
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Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production |
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Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production |
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Martinho, Frederico C. |
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Clinical Oral Investigations |
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Clinical Oral Investigations |
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2014 |
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Martinho, Frederico C. Leite, Fábio R. M. Nascimento, Gustavo G. Cirelli, Joni A. Gomes, Brenda P. F. A. |
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Elektronische Aufsätze |
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Martinho, Frederico C. |
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10.1007/s00784-014-1198-1 |
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clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production |
| title_auth |
Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production |
| abstract |
Introduction This study investigated the presence of different Gram-negative bacterial species and the levels of endotoxins found in primary endodontic infection (PEI), determining their stimulation ability against macrophages through the levels of interleukin (IL)-1, IL-6, IL-10, and tumor necrosis factor alpha (TNF-α), and evaluated their relationship with clinical and radiographic findings. Material and methods Samples were taken from 21 root canals with primary endodontic infection with apical periodontitis (PEIAP). Molecular techniques were used for bacterial detection. Limulus amebocyte lysate assay was used to measure endotoxins. Pro-inflammatory cytokines were measured by ELISA assay. Results All samples were positive for bacterial DNA (21/21). Prevotella nigrescens (57.2 %) was the most frequent species. Higher levels of endotoxins were found in teeth with pain on palpation and exudation (all p < 0.05). Positive correlations were found between endotoxins and the levels of TNF-α and IL-1β, whereas a negative correlation was found between endotoxin and the amount of IL-10 (p < 0.05). Endotoxin levels were found to be a risk factor for exudation and increased the number of Gram-negative bacterial species for the presence of a larger area of bone destruction (all p < 0.05). Conclusion A wide variety of Gram-negative bacterial species are involved in primary endodontic infection, with participation of different Treponema species. Thus, the levels of endotoxins and the number of Gram-negative bacteria species present in root canals were considered risk factors for the severity of endodontic infection. Clinical relevance The present study revealed that Gram-negative bacterial species and endotoxins play an important role in the development of signs/symptoms and the severity of bone destruction, this knowledge is essential for the establishment of an effective therapy. |
| abstractGer |
Introduction This study investigated the presence of different Gram-negative bacterial species and the levels of endotoxins found in primary endodontic infection (PEI), determining their stimulation ability against macrophages through the levels of interleukin (IL)-1, IL-6, IL-10, and tumor necrosis factor alpha (TNF-α), and evaluated their relationship with clinical and radiographic findings. Material and methods Samples were taken from 21 root canals with primary endodontic infection with apical periodontitis (PEIAP). Molecular techniques were used for bacterial detection. Limulus amebocyte lysate assay was used to measure endotoxins. Pro-inflammatory cytokines were measured by ELISA assay. Results All samples were positive for bacterial DNA (21/21). Prevotella nigrescens (57.2 %) was the most frequent species. Higher levels of endotoxins were found in teeth with pain on palpation and exudation (all p < 0.05). Positive correlations were found between endotoxins and the levels of TNF-α and IL-1β, whereas a negative correlation was found between endotoxin and the amount of IL-10 (p < 0.05). Endotoxin levels were found to be a risk factor for exudation and increased the number of Gram-negative bacterial species for the presence of a larger area of bone destruction (all p < 0.05). Conclusion A wide variety of Gram-negative bacterial species are involved in primary endodontic infection, with participation of different Treponema species. Thus, the levels of endotoxins and the number of Gram-negative bacteria species present in root canals were considered risk factors for the severity of endodontic infection. Clinical relevance The present study revealed that Gram-negative bacterial species and endotoxins play an important role in the development of signs/symptoms and the severity of bone destruction, this knowledge is essential for the establishment of an effective therapy. |
| abstract_unstemmed |
Introduction This study investigated the presence of different Gram-negative bacterial species and the levels of endotoxins found in primary endodontic infection (PEI), determining their stimulation ability against macrophages through the levels of interleukin (IL)-1, IL-6, IL-10, and tumor necrosis factor alpha (TNF-α), and evaluated their relationship with clinical and radiographic findings. Material and methods Samples were taken from 21 root canals with primary endodontic infection with apical periodontitis (PEIAP). Molecular techniques were used for bacterial detection. Limulus amebocyte lysate assay was used to measure endotoxins. Pro-inflammatory cytokines were measured by ELISA assay. Results All samples were positive for bacterial DNA (21/21). Prevotella nigrescens (57.2 %) was the most frequent species. Higher levels of endotoxins were found in teeth with pain on palpation and exudation (all p < 0.05). Positive correlations were found between endotoxins and the levels of TNF-α and IL-1β, whereas a negative correlation was found between endotoxin and the amount of IL-10 (p < 0.05). Endotoxin levels were found to be a risk factor for exudation and increased the number of Gram-negative bacterial species for the presence of a larger area of bone destruction (all p < 0.05). Conclusion A wide variety of Gram-negative bacterial species are involved in primary endodontic infection, with participation of different Treponema species. Thus, the levels of endotoxins and the number of Gram-negative bacteria species present in root canals were considered risk factors for the severity of endodontic infection. Clinical relevance The present study revealed that Gram-negative bacterial species and endotoxins play an important role in the development of signs/symptoms and the severity of bone destruction, this knowledge is essential for the establishment of an effective therapy. |
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Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production |
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https://dx.doi.org/10.1007/s00784-014-1198-1 |
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Leite, Fábio R. M. Nascimento, Gustavo G. Cirelli, Joni A. Gomes, Brenda P. F. A. |
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