Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus”
Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing s...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Baumler, David J. [verfasserIn] Hung, Kai-Foong [verfasserIn] Jeong, Kwang Cheol [verfasserIn] Kaspar, Charles W. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2007 |
|---|
| Schlagwörter: |
|---|
| Übergeordnetes Werk: |
Enthalten in: Extremophiles - Springer-Verlag, 2001, 11(2007), 6 vom: 04. Okt., Seite 841-851 |
|---|---|
| Übergeordnetes Werk: |
volume:11 ; year:2007 ; number:6 ; day:04 ; month:10 ; pages:841-851 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00792-007-0108-8 |
|---|
| Katalog-ID: |
SPR00785658X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR00785658X | ||
| 003 | DE-627 | ||
| 005 | 20201124022535.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201005s2007 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00792-007-0108-8 |2 doi | |
| 035 | |a (DE-627)SPR00785658X | ||
| 035 | |a (SPR)s00792-007-0108-8-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Baumler, David J. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” |
| 264 | 1 | |c 2007 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle. | ||
| 650 | 4 | |a Cysteine |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Methanethiol |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Methionine |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Sulfate |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Sulfite reductase |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Thiol methyltransferase |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Hung, Kai-Foong |e verfasserin |4 aut | |
| 700 | 1 | |a Jeong, Kwang Cheol |e verfasserin |4 aut | |
| 700 | 1 | |a Kaspar, Charles W. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Extremophiles |d Springer-Verlag, 2001 |g 11(2007), 6 vom: 04. Okt., Seite 841-851 |w (DE-627)SPR007852657 |7 nnns |
| 773 | 1 | 8 | |g volume:11 |g year:2007 |g number:6 |g day:04 |g month:10 |g pages:841-851 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s00792-007-0108-8 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 951 | |a AR | ||
| 952 | |d 11 |j 2007 |e 6 |b 04 |c 10 |h 841-851 | ||
| author_variant |
d j b dj djb k f h kfh k c j kc kcj c w k cw cwk |
|---|---|
| matchkey_str |
baumlerdavidjhungkaifoongjeongkwangcheol:2007----:rdcinfehntilnvltlslucmonsyhacao |
| hierarchy_sort_str |
2007 |
| publishDate |
2007 |
| allfields |
10.1007/s00792-007-0108-8 doi (DE-627)SPR00785658X (SPR)s00792-007-0108-8-e DE-627 ger DE-627 rakwb eng Baumler, David J. verfasserin aut Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle. Cysteine (dpeaa)DE-He213 Methanethiol (dpeaa)DE-He213 Methionine (dpeaa)DE-He213 Sulfate (dpeaa)DE-He213 Sulfite reductase (dpeaa)DE-He213 Thiol methyltransferase (dpeaa)DE-He213 Hung, Kai-Foong verfasserin aut Jeong, Kwang Cheol verfasserin aut Kaspar, Charles W. verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 11(2007), 6 vom: 04. Okt., Seite 841-851 (DE-627)SPR007852657 nnns volume:11 year:2007 number:6 day:04 month:10 pages:841-851 https://dx.doi.org/10.1007/s00792-007-0108-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 11 2007 6 04 10 841-851 |
| spelling |
10.1007/s00792-007-0108-8 doi (DE-627)SPR00785658X (SPR)s00792-007-0108-8-e DE-627 ger DE-627 rakwb eng Baumler, David J. verfasserin aut Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle. Cysteine (dpeaa)DE-He213 Methanethiol (dpeaa)DE-He213 Methionine (dpeaa)DE-He213 Sulfate (dpeaa)DE-He213 Sulfite reductase (dpeaa)DE-He213 Thiol methyltransferase (dpeaa)DE-He213 Hung, Kai-Foong verfasserin aut Jeong, Kwang Cheol verfasserin aut Kaspar, Charles W. verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 11(2007), 6 vom: 04. Okt., Seite 841-851 (DE-627)SPR007852657 nnns volume:11 year:2007 number:6 day:04 month:10 pages:841-851 https://dx.doi.org/10.1007/s00792-007-0108-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 11 2007 6 04 10 841-851 |
| allfields_unstemmed |
10.1007/s00792-007-0108-8 doi (DE-627)SPR00785658X (SPR)s00792-007-0108-8-e DE-627 ger DE-627 rakwb eng Baumler, David J. verfasserin aut Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle. Cysteine (dpeaa)DE-He213 Methanethiol (dpeaa)DE-He213 Methionine (dpeaa)DE-He213 Sulfate (dpeaa)DE-He213 Sulfite reductase (dpeaa)DE-He213 Thiol methyltransferase (dpeaa)DE-He213 Hung, Kai-Foong verfasserin aut Jeong, Kwang Cheol verfasserin aut Kaspar, Charles W. verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 11(2007), 6 vom: 04. Okt., Seite 841-851 (DE-627)SPR007852657 nnns volume:11 year:2007 number:6 day:04 month:10 pages:841-851 https://dx.doi.org/10.1007/s00792-007-0108-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 11 2007 6 04 10 841-851 |
| allfieldsGer |
10.1007/s00792-007-0108-8 doi (DE-627)SPR00785658X (SPR)s00792-007-0108-8-e DE-627 ger DE-627 rakwb eng Baumler, David J. verfasserin aut Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle. Cysteine (dpeaa)DE-He213 Methanethiol (dpeaa)DE-He213 Methionine (dpeaa)DE-He213 Sulfate (dpeaa)DE-He213 Sulfite reductase (dpeaa)DE-He213 Thiol methyltransferase (dpeaa)DE-He213 Hung, Kai-Foong verfasserin aut Jeong, Kwang Cheol verfasserin aut Kaspar, Charles W. verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 11(2007), 6 vom: 04. Okt., Seite 841-851 (DE-627)SPR007852657 nnns volume:11 year:2007 number:6 day:04 month:10 pages:841-851 https://dx.doi.org/10.1007/s00792-007-0108-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 11 2007 6 04 10 841-851 |
| allfieldsSound |
10.1007/s00792-007-0108-8 doi (DE-627)SPR00785658X (SPR)s00792-007-0108-8-e DE-627 ger DE-627 rakwb eng Baumler, David J. verfasserin aut Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle. Cysteine (dpeaa)DE-He213 Methanethiol (dpeaa)DE-He213 Methionine (dpeaa)DE-He213 Sulfate (dpeaa)DE-He213 Sulfite reductase (dpeaa)DE-He213 Thiol methyltransferase (dpeaa)DE-He213 Hung, Kai-Foong verfasserin aut Jeong, Kwang Cheol verfasserin aut Kaspar, Charles W. verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 11(2007), 6 vom: 04. Okt., Seite 841-851 (DE-627)SPR007852657 nnns volume:11 year:2007 number:6 day:04 month:10 pages:841-851 https://dx.doi.org/10.1007/s00792-007-0108-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 11 2007 6 04 10 841-851 |
| language |
English |
| source |
Enthalten in Extremophiles 11(2007), 6 vom: 04. Okt., Seite 841-851 volume:11 year:2007 number:6 day:04 month:10 pages:841-851 |
| sourceStr |
Enthalten in Extremophiles 11(2007), 6 vom: 04. Okt., Seite 841-851 volume:11 year:2007 number:6 day:04 month:10 pages:841-851 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Cysteine Methanethiol Methionine Sulfate Sulfite reductase Thiol methyltransferase |
| isfreeaccess_bool |
false |
| container_title |
Extremophiles |
| authorswithroles_txt_mv |
Baumler, David J. @@aut@@ Hung, Kai-Foong @@aut@@ Jeong, Kwang Cheol @@aut@@ Kaspar, Charles W. @@aut@@ |
| publishDateDaySort_date |
2007-10-04T00:00:00Z |
| hierarchy_top_id |
SPR007852657 |
| id |
SPR00785658X |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR00785658X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20201124022535.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201005s2007 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00792-007-0108-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR00785658X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00792-007-0108-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Baumler, David J.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus”</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2007</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cysteine</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Methanethiol</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Methionine</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Sulfate</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Sulfite reductase</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Thiol methyltransferase</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hung, Kai-Foong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jeong, Kwang Cheol</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kaspar, Charles W.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Extremophiles</subfield><subfield code="d">Springer-Verlag, 2001</subfield><subfield code="g">11(2007), 6 vom: 04. Okt., Seite 841-851</subfield><subfield code="w">(DE-627)SPR007852657</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:11</subfield><subfield code="g">year:2007</subfield><subfield code="g">number:6</subfield><subfield code="g">day:04</subfield><subfield code="g">month:10</subfield><subfield code="g">pages:841-851</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s00792-007-0108-8</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">11</subfield><subfield code="j">2007</subfield><subfield code="e">6</subfield><subfield code="b">04</subfield><subfield code="c">10</subfield><subfield code="h">841-851</subfield></datafield></record></collection>
|
| author |
Baumler, David J. |
| spellingShingle |
Baumler, David J. misc Cysteine misc Methanethiol misc Methionine misc Sulfate misc Sulfite reductase misc Thiol methyltransferase Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” |
| authorStr |
Baumler, David J. |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)SPR007852657 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut aut |
| collection |
springer |
| remote_str |
true |
| illustrated |
Not Illustrated |
| topic_title |
Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” Cysteine (dpeaa)DE-He213 Methanethiol (dpeaa)DE-He213 Methionine (dpeaa)DE-He213 Sulfate (dpeaa)DE-He213 Sulfite reductase (dpeaa)DE-He213 Thiol methyltransferase (dpeaa)DE-He213 |
| topic |
misc Cysteine misc Methanethiol misc Methionine misc Sulfate misc Sulfite reductase misc Thiol methyltransferase |
| topic_unstemmed |
misc Cysteine misc Methanethiol misc Methionine misc Sulfate misc Sulfite reductase misc Thiol methyltransferase |
| topic_browse |
misc Cysteine misc Methanethiol misc Methionine misc Sulfate misc Sulfite reductase misc Thiol methyltransferase |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| hierarchy_parent_title |
Extremophiles |
| hierarchy_parent_id |
SPR007852657 |
| hierarchy_top_title |
Extremophiles |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)SPR007852657 |
| title |
Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” |
| ctrlnum |
(DE-627)SPR00785658X (SPR)s00792-007-0108-8-e |
| title_full |
Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” |
| author_sort |
Baumler, David J. |
| journal |
Extremophiles |
| journalStr |
Extremophiles |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
2007 |
| contenttype_str_mv |
txt |
| container_start_page |
841 |
| author_browse |
Baumler, David J. Hung, Kai-Foong Jeong, Kwang Cheol Kaspar, Charles W. |
| container_volume |
11 |
| format_se |
Elektronische Aufsätze |
| author-letter |
Baumler, David J. |
| doi_str_mv |
10.1007/s00792-007-0108-8 |
| author2-role |
verfasserin |
| title_sort |
production of methanethiol and volatile sulfur compounds by the archaeon “ferroplasma acidarmanus” |
| title_auth |
Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” |
| abstract |
Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle. |
| abstractGer |
Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle. |
| abstract_unstemmed |
Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle. |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER |
| container_issue |
6 |
| title_short |
Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus” |
| url |
https://dx.doi.org/10.1007/s00792-007-0108-8 |
| remote_bool |
true |
| author2 |
Hung, Kai-Foong Jeong, Kwang Cheol Kaspar, Charles W. |
| author2Str |
Hung, Kai-Foong Jeong, Kwang Cheol Kaspar, Charles W. |
| ppnlink |
SPR007852657 |
| mediatype_str_mv |
c |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1007/s00792-007-0108-8 |
| up_date |
2024-07-03T15:41:48.009Z |
| _version_ |
1803573063069990912 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR00785658X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20201124022535.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201005s2007 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00792-007-0108-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR00785658X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00792-007-0108-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Baumler, David J.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Production of methanethiol and volatile sulfur compounds by the archaeon “Ferroplasma acidarmanus”</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2007</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown “Ferroplasma acidarmanus” showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active “F. acidarmanus” cultures is methanethiol. In “F. acidarmanus” cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO32− indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce $ H_{2} $S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that “F. acidarmanus” produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cysteine</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Methanethiol</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Methionine</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Sulfate</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Sulfite reductase</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Thiol methyltransferase</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hung, Kai-Foong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jeong, Kwang Cheol</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kaspar, Charles W.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Extremophiles</subfield><subfield code="d">Springer-Verlag, 2001</subfield><subfield code="g">11(2007), 6 vom: 04. Okt., Seite 841-851</subfield><subfield code="w">(DE-627)SPR007852657</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:11</subfield><subfield code="g">year:2007</subfield><subfield code="g">number:6</subfield><subfield code="g">day:04</subfield><subfield code="g">month:10</subfield><subfield code="g">pages:841-851</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s00792-007-0108-8</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">11</subfield><subfield code="j">2007</subfield><subfield code="e">6</subfield><subfield code="b">04</subfield><subfield code="c">10</subfield><subfield code="h">841-851</subfield></datafield></record></collection>
|
| score |
7.399703 |