Mutation and reversion frequencies of different Sulfolobus species and strains
Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Berkner, Silvia [verfasserIn] Lipps, Georg [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2008 |
|---|
| Schlagwörter: |
|---|
| Übergeordnetes Werk: |
Enthalten in: Extremophiles - Springer-Verlag, 2001, 12(2008), 2 vom: 05. Jan., Seite 263-270 |
|---|---|
| Übergeordnetes Werk: |
volume:12 ; year:2008 ; number:2 ; day:05 ; month:01 ; pages:263-270 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00792-007-0125-7 |
|---|
| Katalog-ID: |
SPR007856989 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR007856989 | ||
| 003 | DE-627 | ||
| 005 | 20201124022536.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201005s2008 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00792-007-0125-7 |2 doi | |
| 035 | |a (DE-627)SPR007856989 | ||
| 035 | |a (SPR)s00792-007-0125-7-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Berkner, Silvia |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Mutation and reversion frequencies of different Sulfolobus species and strains |
| 264 | 1 | |c 2008 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed. | ||
| 650 | 4 | |a Mutation frequencies |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Reversion frequencies |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Uracil selection |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Lactose selection |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Small-scale fluctuation tests |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Lipps, Georg |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Extremophiles |d Springer-Verlag, 2001 |g 12(2008), 2 vom: 05. Jan., Seite 263-270 |w (DE-627)SPR007852657 |7 nnns |
| 773 | 1 | 8 | |g volume:12 |g year:2008 |g number:2 |g day:05 |g month:01 |g pages:263-270 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s00792-007-0125-7 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 951 | |a AR | ||
| 952 | |d 12 |j 2008 |e 2 |b 05 |c 01 |h 263-270 | ||
| author_variant |
s b sb g l gl |
|---|---|
| matchkey_str |
berknersilvialippsgeorg:2008----:uainnrvrinrqeceodfeetuflb |
| hierarchy_sort_str |
2008 |
| publishDate |
2008 |
| allfields |
10.1007/s00792-007-0125-7 doi (DE-627)SPR007856989 (SPR)s00792-007-0125-7-e DE-627 ger DE-627 rakwb eng Berkner, Silvia verfasserin aut Mutation and reversion frequencies of different Sulfolobus species and strains 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed. Mutation frequencies (dpeaa)DE-He213 Reversion frequencies (dpeaa)DE-He213 Uracil selection (dpeaa)DE-He213 Lactose selection (dpeaa)DE-He213 Small-scale fluctuation tests (dpeaa)DE-He213 Lipps, Georg verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 12(2008), 2 vom: 05. Jan., Seite 263-270 (DE-627)SPR007852657 nnns volume:12 year:2008 number:2 day:05 month:01 pages:263-270 https://dx.doi.org/10.1007/s00792-007-0125-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 12 2008 2 05 01 263-270 |
| spelling |
10.1007/s00792-007-0125-7 doi (DE-627)SPR007856989 (SPR)s00792-007-0125-7-e DE-627 ger DE-627 rakwb eng Berkner, Silvia verfasserin aut Mutation and reversion frequencies of different Sulfolobus species and strains 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed. Mutation frequencies (dpeaa)DE-He213 Reversion frequencies (dpeaa)DE-He213 Uracil selection (dpeaa)DE-He213 Lactose selection (dpeaa)DE-He213 Small-scale fluctuation tests (dpeaa)DE-He213 Lipps, Georg verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 12(2008), 2 vom: 05. Jan., Seite 263-270 (DE-627)SPR007852657 nnns volume:12 year:2008 number:2 day:05 month:01 pages:263-270 https://dx.doi.org/10.1007/s00792-007-0125-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 12 2008 2 05 01 263-270 |
| allfields_unstemmed |
10.1007/s00792-007-0125-7 doi (DE-627)SPR007856989 (SPR)s00792-007-0125-7-e DE-627 ger DE-627 rakwb eng Berkner, Silvia verfasserin aut Mutation and reversion frequencies of different Sulfolobus species and strains 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed. Mutation frequencies (dpeaa)DE-He213 Reversion frequencies (dpeaa)DE-He213 Uracil selection (dpeaa)DE-He213 Lactose selection (dpeaa)DE-He213 Small-scale fluctuation tests (dpeaa)DE-He213 Lipps, Georg verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 12(2008), 2 vom: 05. Jan., Seite 263-270 (DE-627)SPR007852657 nnns volume:12 year:2008 number:2 day:05 month:01 pages:263-270 https://dx.doi.org/10.1007/s00792-007-0125-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 12 2008 2 05 01 263-270 |
| allfieldsGer |
10.1007/s00792-007-0125-7 doi (DE-627)SPR007856989 (SPR)s00792-007-0125-7-e DE-627 ger DE-627 rakwb eng Berkner, Silvia verfasserin aut Mutation and reversion frequencies of different Sulfolobus species and strains 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed. Mutation frequencies (dpeaa)DE-He213 Reversion frequencies (dpeaa)DE-He213 Uracil selection (dpeaa)DE-He213 Lactose selection (dpeaa)DE-He213 Small-scale fluctuation tests (dpeaa)DE-He213 Lipps, Georg verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 12(2008), 2 vom: 05. Jan., Seite 263-270 (DE-627)SPR007852657 nnns volume:12 year:2008 number:2 day:05 month:01 pages:263-270 https://dx.doi.org/10.1007/s00792-007-0125-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 12 2008 2 05 01 263-270 |
| allfieldsSound |
10.1007/s00792-007-0125-7 doi (DE-627)SPR007856989 (SPR)s00792-007-0125-7-e DE-627 ger DE-627 rakwb eng Berkner, Silvia verfasserin aut Mutation and reversion frequencies of different Sulfolobus species and strains 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed. Mutation frequencies (dpeaa)DE-He213 Reversion frequencies (dpeaa)DE-He213 Uracil selection (dpeaa)DE-He213 Lactose selection (dpeaa)DE-He213 Small-scale fluctuation tests (dpeaa)DE-He213 Lipps, Georg verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 12(2008), 2 vom: 05. Jan., Seite 263-270 (DE-627)SPR007852657 nnns volume:12 year:2008 number:2 day:05 month:01 pages:263-270 https://dx.doi.org/10.1007/s00792-007-0125-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 12 2008 2 05 01 263-270 |
| language |
English |
| source |
Enthalten in Extremophiles 12(2008), 2 vom: 05. Jan., Seite 263-270 volume:12 year:2008 number:2 day:05 month:01 pages:263-270 |
| sourceStr |
Enthalten in Extremophiles 12(2008), 2 vom: 05. Jan., Seite 263-270 volume:12 year:2008 number:2 day:05 month:01 pages:263-270 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Mutation frequencies Reversion frequencies Uracil selection Lactose selection Small-scale fluctuation tests |
| isfreeaccess_bool |
false |
| container_title |
Extremophiles |
| authorswithroles_txt_mv |
Berkner, Silvia @@aut@@ Lipps, Georg @@aut@@ |
| publishDateDaySort_date |
2008-01-05T00:00:00Z |
| hierarchy_top_id |
SPR007852657 |
| id |
SPR007856989 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR007856989</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20201124022536.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201005s2008 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00792-007-0125-7</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR007856989</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00792-007-0125-7-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Berkner, Silvia</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Mutation and reversion frequencies of different Sulfolobus species and strains</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2008</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mutation frequencies</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Reversion frequencies</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Uracil selection</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Lactose selection</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Small-scale fluctuation tests</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lipps, Georg</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Extremophiles</subfield><subfield code="d">Springer-Verlag, 2001</subfield><subfield code="g">12(2008), 2 vom: 05. Jan., Seite 263-270</subfield><subfield code="w">(DE-627)SPR007852657</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:12</subfield><subfield code="g">year:2008</subfield><subfield code="g">number:2</subfield><subfield code="g">day:05</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:263-270</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s00792-007-0125-7</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">12</subfield><subfield code="j">2008</subfield><subfield code="e">2</subfield><subfield code="b">05</subfield><subfield code="c">01</subfield><subfield code="h">263-270</subfield></datafield></record></collection>
|
| author |
Berkner, Silvia |
| spellingShingle |
Berkner, Silvia misc Mutation frequencies misc Reversion frequencies misc Uracil selection misc Lactose selection misc Small-scale fluctuation tests Mutation and reversion frequencies of different Sulfolobus species and strains |
| authorStr |
Berkner, Silvia |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)SPR007852657 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut |
| collection |
springer |
| remote_str |
true |
| illustrated |
Not Illustrated |
| topic_title |
Mutation and reversion frequencies of different Sulfolobus species and strains Mutation frequencies (dpeaa)DE-He213 Reversion frequencies (dpeaa)DE-He213 Uracil selection (dpeaa)DE-He213 Lactose selection (dpeaa)DE-He213 Small-scale fluctuation tests (dpeaa)DE-He213 |
| topic |
misc Mutation frequencies misc Reversion frequencies misc Uracil selection misc Lactose selection misc Small-scale fluctuation tests |
| topic_unstemmed |
misc Mutation frequencies misc Reversion frequencies misc Uracil selection misc Lactose selection misc Small-scale fluctuation tests |
| topic_browse |
misc Mutation frequencies misc Reversion frequencies misc Uracil selection misc Lactose selection misc Small-scale fluctuation tests |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| hierarchy_parent_title |
Extremophiles |
| hierarchy_parent_id |
SPR007852657 |
| hierarchy_top_title |
Extremophiles |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)SPR007852657 |
| title |
Mutation and reversion frequencies of different Sulfolobus species and strains |
| ctrlnum |
(DE-627)SPR007856989 (SPR)s00792-007-0125-7-e |
| title_full |
Mutation and reversion frequencies of different Sulfolobus species and strains |
| author_sort |
Berkner, Silvia |
| journal |
Extremophiles |
| journalStr |
Extremophiles |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
2008 |
| contenttype_str_mv |
txt |
| container_start_page |
263 |
| author_browse |
Berkner, Silvia Lipps, Georg |
| container_volume |
12 |
| format_se |
Elektronische Aufsätze |
| author-letter |
Berkner, Silvia |
| doi_str_mv |
10.1007/s00792-007-0125-7 |
| author2-role |
verfasserin |
| title_sort |
mutation and reversion frequencies of different sulfolobus species and strains |
| title_auth |
Mutation and reversion frequencies of different Sulfolobus species and strains |
| abstract |
Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed. |
| abstractGer |
Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed. |
| abstract_unstemmed |
Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed. |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER |
| container_issue |
2 |
| title_short |
Mutation and reversion frequencies of different Sulfolobus species and strains |
| url |
https://dx.doi.org/10.1007/s00792-007-0125-7 |
| remote_bool |
true |
| author2 |
Lipps, Georg |
| author2Str |
Lipps, Georg |
| ppnlink |
SPR007852657 |
| mediatype_str_mv |
c |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1007/s00792-007-0125-7 |
| up_date |
2024-07-03T15:41:55.604Z |
| _version_ |
1803573071038119936 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR007856989</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20201124022536.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201005s2008 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00792-007-0125-7</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR007856989</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00792-007-0125-7-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Berkner, Silvia</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Mutation and reversion frequencies of different Sulfolobus species and strains</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2008</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract We have determined the apparent and actual spontaneous mutation frequencies and rates for different species and strains of the thermoacidophilic crenarchaeote Sulfolobus. The proportion of mutations caused by insertion sequences has also been analyzed. Mutation frequencies for S. islandicus (0.08–0.6 mutations per cell division and $ 10^{7} $ cells) were below those determined for S. solfataricus and comparable to or lower than those for S. acidocaldarius. The proportion of insertion sequence mutations for the S. islandicus strains REN1H1 (9 out of 230) and HVE10/4 (0 out of 24) was found to be considerably lower than in S. solfataricus P1 and P2 and also low in comparison to other S. islandicus strains. Mutants defective in either the pyrEF genes or the lacS gene have been isolated. Their growth phenotype on selective and non-selective medium was examined and the inactivating mutations in either of the genes were determined. In addition the reversion frequencies for these mutants were measured and found to be in the range of <0.6–1.5 mutations per cell division and $ 10^{8} $ cells. However, when being subjected to electroporation as a transformation procedure, increased reversion was observed.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mutation frequencies</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Reversion frequencies</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Uracil selection</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Lactose selection</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Small-scale fluctuation tests</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lipps, Georg</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Extremophiles</subfield><subfield code="d">Springer-Verlag, 2001</subfield><subfield code="g">12(2008), 2 vom: 05. Jan., Seite 263-270</subfield><subfield code="w">(DE-627)SPR007852657</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:12</subfield><subfield code="g">year:2008</subfield><subfield code="g">number:2</subfield><subfield code="g">day:05</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:263-270</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s00792-007-0125-7</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">12</subfield><subfield code="j">2008</subfield><subfield code="e">2</subfield><subfield code="b">05</subfield><subfield code="c">01</subfield><subfield code="h">263-270</subfield></datafield></record></collection>
|
| score |
7.399703 |