Isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the Southwest Indian Ocean Ridge
Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From ar...
Ausführliche Beschreibung
Gespeichert in:
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Chen, Shuangxi [verfasserIn] Shao, Zongze [verfasserIn] |
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E-Artikel |
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Englisch |
| Erschienen: |
2008 |
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Enthalten in: Extremophiles - Springer-Verlag, 2001, 13(2008), 1 vom: 08. Okt., Seite 39-48 |
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| Übergeordnetes Werk: |
volume:13 ; year:2008 ; number:1 ; day:08 ; month:10 ; pages:39-48 |
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10.1007/s00792-008-0195-1 |
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| 520 | |a Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2–80 mM. Phylogenetic analysis based on 16S rRNA showed that they mainly belonged to Proteobacteria and Actinobacteria. Denaturing gradient gel electrophoresis revealed that Microbacterium esteraromaticum was the dominant member in the arsenite enriched communities, and this was reconfirmed by 16S rRNA gene library analyses. Thus, M. esteraromaticum showed highest resistant to arsenite among the detected bacteria. These results indicate that there are quite diverse bacteria of arsenite resistance inhabiting the deep sea sediment, which may play a role in the geobiocycling of arsenic element in marine environments. | ||
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10.1007/s00792-008-0195-1 doi (DE-627)SPR007857705 (SPR)s00792-008-0195-1-e DE-627 ger DE-627 rakwb eng Chen, Shuangxi verfasserin aut Isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the Southwest Indian Ocean Ridge 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2–80 mM. Phylogenetic analysis based on 16S rRNA showed that they mainly belonged to Proteobacteria and Actinobacteria. Denaturing gradient gel electrophoresis revealed that Microbacterium esteraromaticum was the dominant member in the arsenite enriched communities, and this was reconfirmed by 16S rRNA gene library analyses. Thus, M. esteraromaticum showed highest resistant to arsenite among the detected bacteria. These results indicate that there are quite diverse bacteria of arsenite resistance inhabiting the deep sea sediment, which may play a role in the geobiocycling of arsenic element in marine environments. Arsenite (dpeaa)DE-He213 Heavy metal tolerance (dpeaa)DE-He213 Deep sea (dpeaa)DE-He213 Sediment (dpeaa)DE-He213 Biodiversity (dpeaa)DE-He213 Shao, Zongze verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 13(2008), 1 vom: 08. Okt., Seite 39-48 (DE-627)SPR007852657 nnns volume:13 year:2008 number:1 day:08 month:10 pages:39-48 https://dx.doi.org/10.1007/s00792-008-0195-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 13 2008 1 08 10 39-48 |
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10.1007/s00792-008-0195-1 doi (DE-627)SPR007857705 (SPR)s00792-008-0195-1-e DE-627 ger DE-627 rakwb eng Chen, Shuangxi verfasserin aut Isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the Southwest Indian Ocean Ridge 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2–80 mM. Phylogenetic analysis based on 16S rRNA showed that they mainly belonged to Proteobacteria and Actinobacteria. Denaturing gradient gel electrophoresis revealed that Microbacterium esteraromaticum was the dominant member in the arsenite enriched communities, and this was reconfirmed by 16S rRNA gene library analyses. Thus, M. esteraromaticum showed highest resistant to arsenite among the detected bacteria. These results indicate that there are quite diverse bacteria of arsenite resistance inhabiting the deep sea sediment, which may play a role in the geobiocycling of arsenic element in marine environments. Arsenite (dpeaa)DE-He213 Heavy metal tolerance (dpeaa)DE-He213 Deep sea (dpeaa)DE-He213 Sediment (dpeaa)DE-He213 Biodiversity (dpeaa)DE-He213 Shao, Zongze verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 13(2008), 1 vom: 08. Okt., Seite 39-48 (DE-627)SPR007852657 nnns volume:13 year:2008 number:1 day:08 month:10 pages:39-48 https://dx.doi.org/10.1007/s00792-008-0195-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 13 2008 1 08 10 39-48 |
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10.1007/s00792-008-0195-1 doi (DE-627)SPR007857705 (SPR)s00792-008-0195-1-e DE-627 ger DE-627 rakwb eng Chen, Shuangxi verfasserin aut Isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the Southwest Indian Ocean Ridge 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2–80 mM. Phylogenetic analysis based on 16S rRNA showed that they mainly belonged to Proteobacteria and Actinobacteria. Denaturing gradient gel electrophoresis revealed that Microbacterium esteraromaticum was the dominant member in the arsenite enriched communities, and this was reconfirmed by 16S rRNA gene library analyses. Thus, M. esteraromaticum showed highest resistant to arsenite among the detected bacteria. These results indicate that there are quite diverse bacteria of arsenite resistance inhabiting the deep sea sediment, which may play a role in the geobiocycling of arsenic element in marine environments. Arsenite (dpeaa)DE-He213 Heavy metal tolerance (dpeaa)DE-He213 Deep sea (dpeaa)DE-He213 Sediment (dpeaa)DE-He213 Biodiversity (dpeaa)DE-He213 Shao, Zongze verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 13(2008), 1 vom: 08. Okt., Seite 39-48 (DE-627)SPR007852657 nnns volume:13 year:2008 number:1 day:08 month:10 pages:39-48 https://dx.doi.org/10.1007/s00792-008-0195-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 13 2008 1 08 10 39-48 |
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10.1007/s00792-008-0195-1 doi (DE-627)SPR007857705 (SPR)s00792-008-0195-1-e DE-627 ger DE-627 rakwb eng Chen, Shuangxi verfasserin aut Isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the Southwest Indian Ocean Ridge 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2–80 mM. Phylogenetic analysis based on 16S rRNA showed that they mainly belonged to Proteobacteria and Actinobacteria. Denaturing gradient gel electrophoresis revealed that Microbacterium esteraromaticum was the dominant member in the arsenite enriched communities, and this was reconfirmed by 16S rRNA gene library analyses. Thus, M. esteraromaticum showed highest resistant to arsenite among the detected bacteria. These results indicate that there are quite diverse bacteria of arsenite resistance inhabiting the deep sea sediment, which may play a role in the geobiocycling of arsenic element in marine environments. Arsenite (dpeaa)DE-He213 Heavy metal tolerance (dpeaa)DE-He213 Deep sea (dpeaa)DE-He213 Sediment (dpeaa)DE-He213 Biodiversity (dpeaa)DE-He213 Shao, Zongze verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 13(2008), 1 vom: 08. Okt., Seite 39-48 (DE-627)SPR007852657 nnns volume:13 year:2008 number:1 day:08 month:10 pages:39-48 https://dx.doi.org/10.1007/s00792-008-0195-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 13 2008 1 08 10 39-48 |
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10.1007/s00792-008-0195-1 doi (DE-627)SPR007857705 (SPR)s00792-008-0195-1-e DE-627 ger DE-627 rakwb eng Chen, Shuangxi verfasserin aut Isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the Southwest Indian Ocean Ridge 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2–80 mM. Phylogenetic analysis based on 16S rRNA showed that they mainly belonged to Proteobacteria and Actinobacteria. Denaturing gradient gel electrophoresis revealed that Microbacterium esteraromaticum was the dominant member in the arsenite enriched communities, and this was reconfirmed by 16S rRNA gene library analyses. Thus, M. esteraromaticum showed highest resistant to arsenite among the detected bacteria. These results indicate that there are quite diverse bacteria of arsenite resistance inhabiting the deep sea sediment, which may play a role in the geobiocycling of arsenic element in marine environments. Arsenite (dpeaa)DE-He213 Heavy metal tolerance (dpeaa)DE-He213 Deep sea (dpeaa)DE-He213 Sediment (dpeaa)DE-He213 Biodiversity (dpeaa)DE-He213 Shao, Zongze verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 13(2008), 1 vom: 08. Okt., Seite 39-48 (DE-627)SPR007852657 nnns volume:13 year:2008 number:1 day:08 month:10 pages:39-48 https://dx.doi.org/10.1007/s00792-008-0195-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 13 2008 1 08 10 39-48 |
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Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2–80 mM. Phylogenetic analysis based on 16S rRNA showed that they mainly belonged to Proteobacteria and Actinobacteria. Denaturing gradient gel electrophoresis revealed that Microbacterium esteraromaticum was the dominant member in the arsenite enriched communities, and this was reconfirmed by 16S rRNA gene library analyses. Thus, M. esteraromaticum showed highest resistant to arsenite among the detected bacteria. These results indicate that there are quite diverse bacteria of arsenite resistance inhabiting the deep sea sediment, which may play a role in the geobiocycling of arsenic element in marine environments. |
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Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2–80 mM. Phylogenetic analysis based on 16S rRNA showed that they mainly belonged to Proteobacteria and Actinobacteria. Denaturing gradient gel electrophoresis revealed that Microbacterium esteraromaticum was the dominant member in the arsenite enriched communities, and this was reconfirmed by 16S rRNA gene library analyses. Thus, M. esteraromaticum showed highest resistant to arsenite among the detected bacteria. These results indicate that there are quite diverse bacteria of arsenite resistance inhabiting the deep sea sediment, which may play a role in the geobiocycling of arsenic element in marine environments. |
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Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2–80 mM. Phylogenetic analysis based on 16S rRNA showed that they mainly belonged to Proteobacteria and Actinobacteria. Denaturing gradient gel electrophoresis revealed that Microbacterium esteraromaticum was the dominant member in the arsenite enriched communities, and this was reconfirmed by 16S rRNA gene library analyses. Thus, M. esteraromaticum showed highest resistant to arsenite among the detected bacteria. These results indicate that there are quite diverse bacteria of arsenite resistance inhabiting the deep sea sediment, which may play a role in the geobiocycling of arsenic element in marine environments. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR007857705</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20201124022537.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201005s2008 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00792-008-0195-1</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR007857705</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00792-008-0195-1-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Chen, Shuangxi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the Southwest Indian Ocean Ridge</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2008</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Microorganisms play an important role in the geobiocycling of arsenic element. However, little is known about the bacteria involved in this process in oceanic environments. In this report, arsenite-resistant bacteria were detected in deep-sea sediments on the Southwest Indian Ridge. From arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2–80 mM. Phylogenetic analysis based on 16S rRNA showed that they mainly belonged to Proteobacteria and Actinobacteria. Denaturing gradient gel electrophoresis revealed that Microbacterium esteraromaticum was the dominant member in the arsenite enriched communities, and this was reconfirmed by 16S rRNA gene library analyses. Thus, M. esteraromaticum showed highest resistant to arsenite among the detected bacteria. These results indicate that there are quite diverse bacteria of arsenite resistance inhabiting the deep sea sediment, which may play a role in the geobiocycling of arsenic element in marine environments.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Arsenite</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Heavy metal tolerance</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Deep sea</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Sediment</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biodiversity</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Shao, Zongze</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Extremophiles</subfield><subfield code="d">Springer-Verlag, 2001</subfield><subfield code="g">13(2008), 1 vom: 08. 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