Applications of flow cytometry in environmental microbiology and biotechnology
Abstract Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Bergquist, Peter L. [verfasserIn] Hardiman, Elizabeth M. [verfasserIn] Ferrari, Belinda C. [verfasserIn] Winsley, Tristrom [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Extremophiles - Springer-Verlag, 2001, 13(2009), 3 vom: 20. März, Seite 389-401 |
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| Übergeordnetes Werk: |
volume:13 ; year:2009 ; number:3 ; day:20 ; month:03 ; pages:389-401 |
| Links: |
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| DOI / URN: |
10.1007/s00792-009-0236-4 |
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| 520 | |a Abstract Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-in-water emulsions or with individual genes in transcriptional/translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedure known as random or neutral drift where screening and selection is based on the retention of some predetermined level of activity through multiple rounds of mutagenesis. | ||
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10.1007/s00792-009-0236-4 doi (DE-627)SPR007858167 (SPR)s00792-009-0236-4-e DE-627 ger DE-627 rakwb eng Bergquist, Peter L. verfasserin aut Applications of flow cytometry in environmental microbiology and biotechnology 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-in-water emulsions or with individual genes in transcriptional/translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedure known as random or neutral drift where screening and selection is based on the retention of some predetermined level of activity through multiple rounds of mutagenesis. Fluorescence-activated cell sorting (dpeaa)DE-He213 Directed evolution (dpeaa)DE-He213 In vitro compartmentalisation (dpeaa)DE-He213 Non-culturable bacteria (dpeaa)DE-He213 Single cell analysis (dpeaa)DE-He213 Random (neutral) drift (dpeaa)DE-He213 Random mutagenesis (dpeaa)DE-He213 Protein optimisation (dpeaa)DE-He213 Hardiman, Elizabeth M. verfasserin aut Ferrari, Belinda C. verfasserin aut Winsley, Tristrom verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 13(2009), 3 vom: 20. März, Seite 389-401 (DE-627)SPR007852657 nnns volume:13 year:2009 number:3 day:20 month:03 pages:389-401 https://dx.doi.org/10.1007/s00792-009-0236-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 13 2009 3 20 03 389-401 |
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10.1007/s00792-009-0236-4 doi (DE-627)SPR007858167 (SPR)s00792-009-0236-4-e DE-627 ger DE-627 rakwb eng Bergquist, Peter L. verfasserin aut Applications of flow cytometry in environmental microbiology and biotechnology 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-in-water emulsions or with individual genes in transcriptional/translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedure known as random or neutral drift where screening and selection is based on the retention of some predetermined level of activity through multiple rounds of mutagenesis. Fluorescence-activated cell sorting (dpeaa)DE-He213 Directed evolution (dpeaa)DE-He213 In vitro compartmentalisation (dpeaa)DE-He213 Non-culturable bacteria (dpeaa)DE-He213 Single cell analysis (dpeaa)DE-He213 Random (neutral) drift (dpeaa)DE-He213 Random mutagenesis (dpeaa)DE-He213 Protein optimisation (dpeaa)DE-He213 Hardiman, Elizabeth M. verfasserin aut Ferrari, Belinda C. verfasserin aut Winsley, Tristrom verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 13(2009), 3 vom: 20. März, Seite 389-401 (DE-627)SPR007852657 nnns volume:13 year:2009 number:3 day:20 month:03 pages:389-401 https://dx.doi.org/10.1007/s00792-009-0236-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 13 2009 3 20 03 389-401 |
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10.1007/s00792-009-0236-4 doi (DE-627)SPR007858167 (SPR)s00792-009-0236-4-e DE-627 ger DE-627 rakwb eng Bergquist, Peter L. verfasserin aut Applications of flow cytometry in environmental microbiology and biotechnology 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-in-water emulsions or with individual genes in transcriptional/translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedure known as random or neutral drift where screening and selection is based on the retention of some predetermined level of activity through multiple rounds of mutagenesis. Fluorescence-activated cell sorting (dpeaa)DE-He213 Directed evolution (dpeaa)DE-He213 In vitro compartmentalisation (dpeaa)DE-He213 Non-culturable bacteria (dpeaa)DE-He213 Single cell analysis (dpeaa)DE-He213 Random (neutral) drift (dpeaa)DE-He213 Random mutagenesis (dpeaa)DE-He213 Protein optimisation (dpeaa)DE-He213 Hardiman, Elizabeth M. verfasserin aut Ferrari, Belinda C. verfasserin aut Winsley, Tristrom verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 13(2009), 3 vom: 20. März, Seite 389-401 (DE-627)SPR007852657 nnns volume:13 year:2009 number:3 day:20 month:03 pages:389-401 https://dx.doi.org/10.1007/s00792-009-0236-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 13 2009 3 20 03 389-401 |
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10.1007/s00792-009-0236-4 doi (DE-627)SPR007858167 (SPR)s00792-009-0236-4-e DE-627 ger DE-627 rakwb eng Bergquist, Peter L. verfasserin aut Applications of flow cytometry in environmental microbiology and biotechnology 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-in-water emulsions or with individual genes in transcriptional/translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedure known as random or neutral drift where screening and selection is based on the retention of some predetermined level of activity through multiple rounds of mutagenesis. Fluorescence-activated cell sorting (dpeaa)DE-He213 Directed evolution (dpeaa)DE-He213 In vitro compartmentalisation (dpeaa)DE-He213 Non-culturable bacteria (dpeaa)DE-He213 Single cell analysis (dpeaa)DE-He213 Random (neutral) drift (dpeaa)DE-He213 Random mutagenesis (dpeaa)DE-He213 Protein optimisation (dpeaa)DE-He213 Hardiman, Elizabeth M. verfasserin aut Ferrari, Belinda C. verfasserin aut Winsley, Tristrom verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 13(2009), 3 vom: 20. März, Seite 389-401 (DE-627)SPR007852657 nnns volume:13 year:2009 number:3 day:20 month:03 pages:389-401 https://dx.doi.org/10.1007/s00792-009-0236-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 13 2009 3 20 03 389-401 |
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Applications of flow cytometry in environmental microbiology and biotechnology |
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Abstract Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-in-water emulsions or with individual genes in transcriptional/translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedure known as random or neutral drift where screening and selection is based on the retention of some predetermined level of activity through multiple rounds of mutagenesis. |
| abstractGer |
Abstract Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-in-water emulsions or with individual genes in transcriptional/translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedure known as random or neutral drift where screening and selection is based on the retention of some predetermined level of activity through multiple rounds of mutagenesis. |
| abstract_unstemmed |
Abstract Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-in-water emulsions or with individual genes in transcriptional/translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedure known as random or neutral drift where screening and selection is based on the retention of some predetermined level of activity through multiple rounds of mutagenesis. |
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| title_short |
Applications of flow cytometry in environmental microbiology and biotechnology |
| url |
https://dx.doi.org/10.1007/s00792-009-0236-4 |
| remote_bool |
true |
| author2 |
Hardiman, Elizabeth M. Ferrari, Belinda C. Winsley, Tristrom |
| author2Str |
Hardiman, Elizabeth M. Ferrari, Belinda C. Winsley, Tristrom |
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SPR007852657 |
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| hochschulschrift_bool |
false |
| doi_str |
10.1007/s00792-009-0236-4 |
| up_date |
2024-07-03T15:42:19.703Z |
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7.402815 |