Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Abstract The novel species Halomonas zhaodongensis NEAU-ST10-$ 25^{T} $ recently identified by our group is a moderate halophile which can grow at the range of 0–2.5 M NaCl (optimum 0.5 M) and pH 6–12 (optimum pH 9). To explore its halo-alkaline tolerant mechanism, genomic DNA was screened from NEAU...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Meng, Lin [verfasserIn] Hong, Shan [verfasserIn] Liu, Henan [verfasserIn] Huang, Haipeng [verfasserIn] Sun, Hao [verfasserIn] Xu, Tong [verfasserIn] Jiang, Juquan [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Extremophiles - Springer-Verlag, 2001, 18(2014), 6 vom: 05. Juli, Seite 963-972 |
|---|---|
| Übergeordnetes Werk: |
volume:18 ; year:2014 ; number:6 ; day:05 ; month:07 ; pages:963-972 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00792-014-0666-5 |
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SPR007862954 |
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| 520 | |a Abstract The novel species Halomonas zhaodongensis NEAU-ST10-$ 25^{T} $ recently identified by our group is a moderate halophile which can grow at the range of 0–2.5 M NaCl (optimum 0.5 M) and pH 6–12 (optimum pH 9). To explore its halo-alkaline tolerant mechanism, genomic DNA was screened from NEAU-ST10-$ 25^{T} $ in this study for $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporter genes by selection in Escherichia coli KNabc lacking three major $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporters. One mrp operon could confer tolerance of E. coli KNabc to 0.8 M NaCl and 100 mM LiCl, and an alkaline pH. This operon was previously mainly designated mrp (also mnh, pha or sha) due to its multiple resistance and pH-related activity. Here, we will also use mrp to designate the homolog from H. zhaodongensis (Hz_mrp). Sequence analysis and protein alignment showed that Hz_mrp should belong to Group 1 mrp operons. Further phylogenetic analysis reveals that Hz_Mrp system should represent a novel sub-class of Group 1 Mrp systems. This was confirmed by a significant difference in pH-dependent activity profile or the specificity and affinity for the transported monovalent cations between Hz_Mrp system and all the known Mrp systems. Therefore, we propose that Hz_Mrp should be categorized as a novel Group 1 Mrp system. | ||
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10.1007/s00792-014-0666-5 doi (DE-627)SPR007862954 (SPR)s00792-014-0666-5-e DE-627 ger DE-627 rakwb eng Meng, Lin verfasserin aut Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The novel species Halomonas zhaodongensis NEAU-ST10-$ 25^{T} $ recently identified by our group is a moderate halophile which can grow at the range of 0–2.5 M NaCl (optimum 0.5 M) and pH 6–12 (optimum pH 9). To explore its halo-alkaline tolerant mechanism, genomic DNA was screened from NEAU-ST10-$ 25^{T} $ in this study for $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporter genes by selection in Escherichia coli KNabc lacking three major $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporters. One mrp operon could confer tolerance of E. coli KNabc to 0.8 M NaCl and 100 mM LiCl, and an alkaline pH. This operon was previously mainly designated mrp (also mnh, pha or sha) due to its multiple resistance and pH-related activity. Here, we will also use mrp to designate the homolog from H. zhaodongensis (Hz_mrp). Sequence analysis and protein alignment showed that Hz_mrp should belong to Group 1 mrp operons. Further phylogenetic analysis reveals that Hz_Mrp system should represent a novel sub-class of Group 1 Mrp systems. This was confirmed by a significant difference in pH-dependent activity profile or the specificity and affinity for the transported monovalent cations between Hz_Mrp system and all the known Mrp systems. Therefore, we propose that Hz_Mrp should be categorized as a novel Group 1 Mrp system. Cloning (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Novel Mrp system (dpeaa)DE-He213 Halophilic bacterium (dpeaa)DE-He213 Hong, Shan verfasserin aut Liu, Henan verfasserin aut Huang, Haipeng verfasserin aut Sun, Hao verfasserin aut Xu, Tong verfasserin aut Jiang, Juquan verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 18(2014), 6 vom: 05. Juli, Seite 963-972 (DE-627)SPR007852657 nnns volume:18 year:2014 number:6 day:05 month:07 pages:963-972 https://dx.doi.org/10.1007/s00792-014-0666-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 18 2014 6 05 07 963-972 |
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10.1007/s00792-014-0666-5 doi (DE-627)SPR007862954 (SPR)s00792-014-0666-5-e DE-627 ger DE-627 rakwb eng Meng, Lin verfasserin aut Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The novel species Halomonas zhaodongensis NEAU-ST10-$ 25^{T} $ recently identified by our group is a moderate halophile which can grow at the range of 0–2.5 M NaCl (optimum 0.5 M) and pH 6–12 (optimum pH 9). To explore its halo-alkaline tolerant mechanism, genomic DNA was screened from NEAU-ST10-$ 25^{T} $ in this study for $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporter genes by selection in Escherichia coli KNabc lacking three major $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporters. One mrp operon could confer tolerance of E. coli KNabc to 0.8 M NaCl and 100 mM LiCl, and an alkaline pH. This operon was previously mainly designated mrp (also mnh, pha or sha) due to its multiple resistance and pH-related activity. Here, we will also use mrp to designate the homolog from H. zhaodongensis (Hz_mrp). Sequence analysis and protein alignment showed that Hz_mrp should belong to Group 1 mrp operons. Further phylogenetic analysis reveals that Hz_Mrp system should represent a novel sub-class of Group 1 Mrp systems. This was confirmed by a significant difference in pH-dependent activity profile or the specificity and affinity for the transported monovalent cations between Hz_Mrp system and all the known Mrp systems. Therefore, we propose that Hz_Mrp should be categorized as a novel Group 1 Mrp system. Cloning (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Novel Mrp system (dpeaa)DE-He213 Halophilic bacterium (dpeaa)DE-He213 Hong, Shan verfasserin aut Liu, Henan verfasserin aut Huang, Haipeng verfasserin aut Sun, Hao verfasserin aut Xu, Tong verfasserin aut Jiang, Juquan verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 18(2014), 6 vom: 05. Juli, Seite 963-972 (DE-627)SPR007852657 nnns volume:18 year:2014 number:6 day:05 month:07 pages:963-972 https://dx.doi.org/10.1007/s00792-014-0666-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 18 2014 6 05 07 963-972 |
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10.1007/s00792-014-0666-5 doi (DE-627)SPR007862954 (SPR)s00792-014-0666-5-e DE-627 ger DE-627 rakwb eng Meng, Lin verfasserin aut Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The novel species Halomonas zhaodongensis NEAU-ST10-$ 25^{T} $ recently identified by our group is a moderate halophile which can grow at the range of 0–2.5 M NaCl (optimum 0.5 M) and pH 6–12 (optimum pH 9). To explore its halo-alkaline tolerant mechanism, genomic DNA was screened from NEAU-ST10-$ 25^{T} $ in this study for $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporter genes by selection in Escherichia coli KNabc lacking three major $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporters. One mrp operon could confer tolerance of E. coli KNabc to 0.8 M NaCl and 100 mM LiCl, and an alkaline pH. This operon was previously mainly designated mrp (also mnh, pha or sha) due to its multiple resistance and pH-related activity. Here, we will also use mrp to designate the homolog from H. zhaodongensis (Hz_mrp). Sequence analysis and protein alignment showed that Hz_mrp should belong to Group 1 mrp operons. Further phylogenetic analysis reveals that Hz_Mrp system should represent a novel sub-class of Group 1 Mrp systems. This was confirmed by a significant difference in pH-dependent activity profile or the specificity and affinity for the transported monovalent cations between Hz_Mrp system and all the known Mrp systems. Therefore, we propose that Hz_Mrp should be categorized as a novel Group 1 Mrp system. Cloning (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Novel Mrp system (dpeaa)DE-He213 Halophilic bacterium (dpeaa)DE-He213 Hong, Shan verfasserin aut Liu, Henan verfasserin aut Huang, Haipeng verfasserin aut Sun, Hao verfasserin aut Xu, Tong verfasserin aut Jiang, Juquan verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 18(2014), 6 vom: 05. Juli, Seite 963-972 (DE-627)SPR007852657 nnns volume:18 year:2014 number:6 day:05 month:07 pages:963-972 https://dx.doi.org/10.1007/s00792-014-0666-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 18 2014 6 05 07 963-972 |
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10.1007/s00792-014-0666-5 doi (DE-627)SPR007862954 (SPR)s00792-014-0666-5-e DE-627 ger DE-627 rakwb eng Meng, Lin verfasserin aut Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The novel species Halomonas zhaodongensis NEAU-ST10-$ 25^{T} $ recently identified by our group is a moderate halophile which can grow at the range of 0–2.5 M NaCl (optimum 0.5 M) and pH 6–12 (optimum pH 9). To explore its halo-alkaline tolerant mechanism, genomic DNA was screened from NEAU-ST10-$ 25^{T} $ in this study for $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporter genes by selection in Escherichia coli KNabc lacking three major $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporters. One mrp operon could confer tolerance of E. coli KNabc to 0.8 M NaCl and 100 mM LiCl, and an alkaline pH. This operon was previously mainly designated mrp (also mnh, pha or sha) due to its multiple resistance and pH-related activity. Here, we will also use mrp to designate the homolog from H. zhaodongensis (Hz_mrp). Sequence analysis and protein alignment showed that Hz_mrp should belong to Group 1 mrp operons. Further phylogenetic analysis reveals that Hz_Mrp system should represent a novel sub-class of Group 1 Mrp systems. This was confirmed by a significant difference in pH-dependent activity profile or the specificity and affinity for the transported monovalent cations between Hz_Mrp system and all the known Mrp systems. Therefore, we propose that Hz_Mrp should be categorized as a novel Group 1 Mrp system. Cloning (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Novel Mrp system (dpeaa)DE-He213 Halophilic bacterium (dpeaa)DE-He213 Hong, Shan verfasserin aut Liu, Henan verfasserin aut Huang, Haipeng verfasserin aut Sun, Hao verfasserin aut Xu, Tong verfasserin aut Jiang, Juquan verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 18(2014), 6 vom: 05. Juli, Seite 963-972 (DE-627)SPR007852657 nnns volume:18 year:2014 number:6 day:05 month:07 pages:963-972 https://dx.doi.org/10.1007/s00792-014-0666-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 18 2014 6 05 07 963-972 |
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10.1007/s00792-014-0666-5 doi (DE-627)SPR007862954 (SPR)s00792-014-0666-5-e DE-627 ger DE-627 rakwb eng Meng, Lin verfasserin aut Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The novel species Halomonas zhaodongensis NEAU-ST10-$ 25^{T} $ recently identified by our group is a moderate halophile which can grow at the range of 0–2.5 M NaCl (optimum 0.5 M) and pH 6–12 (optimum pH 9). To explore its halo-alkaline tolerant mechanism, genomic DNA was screened from NEAU-ST10-$ 25^{T} $ in this study for $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporter genes by selection in Escherichia coli KNabc lacking three major $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporters. One mrp operon could confer tolerance of E. coli KNabc to 0.8 M NaCl and 100 mM LiCl, and an alkaline pH. This operon was previously mainly designated mrp (also mnh, pha or sha) due to its multiple resistance and pH-related activity. Here, we will also use mrp to designate the homolog from H. zhaodongensis (Hz_mrp). Sequence analysis and protein alignment showed that Hz_mrp should belong to Group 1 mrp operons. Further phylogenetic analysis reveals that Hz_Mrp system should represent a novel sub-class of Group 1 Mrp systems. This was confirmed by a significant difference in pH-dependent activity profile or the specificity and affinity for the transported monovalent cations between Hz_Mrp system and all the known Mrp systems. Therefore, we propose that Hz_Mrp should be categorized as a novel Group 1 Mrp system. Cloning (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Novel Mrp system (dpeaa)DE-He213 Halophilic bacterium (dpeaa)DE-He213 Hong, Shan verfasserin aut Liu, Henan verfasserin aut Huang, Haipeng verfasserin aut Sun, Hao verfasserin aut Xu, Tong verfasserin aut Jiang, Juquan verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 18(2014), 6 vom: 05. Juli, Seite 963-972 (DE-627)SPR007852657 nnns volume:18 year:2014 number:6 day:05 month:07 pages:963-972 https://dx.doi.org/10.1007/s00792-014-0666-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 18 2014 6 05 07 963-972 |
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Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis Cloning (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Novel Mrp system (dpeaa)DE-He213 Halophilic bacterium (dpeaa)DE-He213 |
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Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis |
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Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis |
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Meng, Lin |
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2014 |
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Meng, Lin Hong, Shan Liu, Henan Huang, Haipeng Sun, Hao Xu, Tong Jiang, Juquan |
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Elektronische Aufsätze |
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10.1007/s00792-014-0666-5 |
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cloning and identification of group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile halomonas zhaodongensis |
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Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis |
| abstract |
Abstract The novel species Halomonas zhaodongensis NEAU-ST10-$ 25^{T} $ recently identified by our group is a moderate halophile which can grow at the range of 0–2.5 M NaCl (optimum 0.5 M) and pH 6–12 (optimum pH 9). To explore its halo-alkaline tolerant mechanism, genomic DNA was screened from NEAU-ST10-$ 25^{T} $ in this study for $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporter genes by selection in Escherichia coli KNabc lacking three major $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporters. One mrp operon could confer tolerance of E. coli KNabc to 0.8 M NaCl and 100 mM LiCl, and an alkaline pH. This operon was previously mainly designated mrp (also mnh, pha or sha) due to its multiple resistance and pH-related activity. Here, we will also use mrp to designate the homolog from H. zhaodongensis (Hz_mrp). Sequence analysis and protein alignment showed that Hz_mrp should belong to Group 1 mrp operons. Further phylogenetic analysis reveals that Hz_Mrp system should represent a novel sub-class of Group 1 Mrp systems. This was confirmed by a significant difference in pH-dependent activity profile or the specificity and affinity for the transported monovalent cations between Hz_Mrp system and all the known Mrp systems. Therefore, we propose that Hz_Mrp should be categorized as a novel Group 1 Mrp system. |
| abstractGer |
Abstract The novel species Halomonas zhaodongensis NEAU-ST10-$ 25^{T} $ recently identified by our group is a moderate halophile which can grow at the range of 0–2.5 M NaCl (optimum 0.5 M) and pH 6–12 (optimum pH 9). To explore its halo-alkaline tolerant mechanism, genomic DNA was screened from NEAU-ST10-$ 25^{T} $ in this study for $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporter genes by selection in Escherichia coli KNabc lacking three major $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporters. One mrp operon could confer tolerance of E. coli KNabc to 0.8 M NaCl and 100 mM LiCl, and an alkaline pH. This operon was previously mainly designated mrp (also mnh, pha or sha) due to its multiple resistance and pH-related activity. Here, we will also use mrp to designate the homolog from H. zhaodongensis (Hz_mrp). Sequence analysis and protein alignment showed that Hz_mrp should belong to Group 1 mrp operons. Further phylogenetic analysis reveals that Hz_Mrp system should represent a novel sub-class of Group 1 Mrp systems. This was confirmed by a significant difference in pH-dependent activity profile or the specificity and affinity for the transported monovalent cations between Hz_Mrp system and all the known Mrp systems. Therefore, we propose that Hz_Mrp should be categorized as a novel Group 1 Mrp system. |
| abstract_unstemmed |
Abstract The novel species Halomonas zhaodongensis NEAU-ST10-$ 25^{T} $ recently identified by our group is a moderate halophile which can grow at the range of 0–2.5 M NaCl (optimum 0.5 M) and pH 6–12 (optimum pH 9). To explore its halo-alkaline tolerant mechanism, genomic DNA was screened from NEAU-ST10-$ 25^{T} $ in this study for $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporter genes by selection in Escherichia coli KNabc lacking three major $ Na^{+} $($ Li^{+} $)/$ H^{+} $ antiporters. One mrp operon could confer tolerance of E. coli KNabc to 0.8 M NaCl and 100 mM LiCl, and an alkaline pH. This operon was previously mainly designated mrp (also mnh, pha or sha) due to its multiple resistance and pH-related activity. Here, we will also use mrp to designate the homolog from H. zhaodongensis (Hz_mrp). Sequence analysis and protein alignment showed that Hz_mrp should belong to Group 1 mrp operons. Further phylogenetic analysis reveals that Hz_Mrp system should represent a novel sub-class of Group 1 Mrp systems. This was confirmed by a significant difference in pH-dependent activity profile or the specificity and affinity for the transported monovalent cations between Hz_Mrp system and all the known Mrp systems. Therefore, we propose that Hz_Mrp should be categorized as a novel Group 1 Mrp system. |
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Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis |
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Hong, Shan Liu, Henan Huang, Haipeng Sun, Hao Xu, Tong Jiang, Juquan |
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