Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis
Abstract The redox-responsive regulator SurR in the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus kodakarensis binds to the SurR-binding consensus sequence (SBS) by responding to the presence of elemental sulfur. Here we constructed a surR gene disruption strain (DTS) in T. kodakare...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Hidese, Ryota [verfasserIn] Yamashita, Keita [verfasserIn] Kawazuma, Kohei [verfasserIn] Kanai, Tamotsu [verfasserIn] Atomi, Haruyuki [verfasserIn] Imanaka, Tadayuki [verfasserIn] Fujiwara, Shinsuke [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Extremophiles - Springer-Verlag, 2001, 21(2017), 5 vom: 07. Juli, Seite 903-917 |
|---|---|
| Übergeordnetes Werk: |
volume:21 ; year:2017 ; number:5 ; day:07 ; month:07 ; pages:903-917 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00792-017-0952-0 |
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| Katalog-ID: |
SPR007866046 |
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| 520 | |a Abstract The redox-responsive regulator SurR in the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus kodakarensis binds to the SurR-binding consensus sequence (SBS) by responding to the presence of elemental sulfur. Here we constructed a surR gene disruption strain (DTS) in T. kodakarensis, and identified the genes that were under SurR control by comparing the transcriptomes of DTS and parent strains. Among these genes, transcript levels of ferredoxin:$ NADP^{+} $ oxidoreductases 1 and 2 (FNOR1 and FNOR2) genes displayed opposite responses to surR deletion, indicating that SurR repressed FNOR1 transcription while enhancing FNOR2 transcription. Each promoter region contains an SBS upstream (uSBS) and downstream (dSBS) of TATA. In addition to in vitro binding assays, we examined the roles of each SBS in vivo. In FNOR1, mutations in either one of the SBSs resulted in a complete loss of repression, indicating that the presence of both SBSs was essential for repression. In FNOR2, uSBS indeed functioned to enhance gene expression, whereas dSBS functioned in gene repression. SurR bound to uSBS2 of FNOR2 more efficiently than to dSBS2 in vitro, which may explain why SurR overall enhances FNOR2 transcription. Further analyses indicated the importance in the distance between uSBS and TATA for transcriptional activation in FNOR2. | ||
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10.1007/s00792-017-0952-0 doi (DE-627)SPR007866046 (SPR)s00792-017-0952-0-e DE-627 ger DE-627 rakwb eng Hidese, Ryota verfasserin aut Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The redox-responsive regulator SurR in the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus kodakarensis binds to the SurR-binding consensus sequence (SBS) by responding to the presence of elemental sulfur. Here we constructed a surR gene disruption strain (DTS) in T. kodakarensis, and identified the genes that were under SurR control by comparing the transcriptomes of DTS and parent strains. Among these genes, transcript levels of ferredoxin:$ NADP^{+} $ oxidoreductases 1 and 2 (FNOR1 and FNOR2) genes displayed opposite responses to surR deletion, indicating that SurR repressed FNOR1 transcription while enhancing FNOR2 transcription. Each promoter region contains an SBS upstream (uSBS) and downstream (dSBS) of TATA. In addition to in vitro binding assays, we examined the roles of each SBS in vivo. In FNOR1, mutations in either one of the SBSs resulted in a complete loss of repression, indicating that the presence of both SBSs was essential for repression. In FNOR2, uSBS indeed functioned to enhance gene expression, whereas dSBS functioned in gene repression. SurR bound to uSBS2 of FNOR2 more efficiently than to dSBS2 in vitro, which may explain why SurR overall enhances FNOR2 transcription. Further analyses indicated the importance in the distance between uSBS and TATA for transcriptional activation in FNOR2. Transcriptional regulator (dpeaa)DE-He213 Redox-responsive (dpeaa)DE-He213 Regulation mechanism (dpeaa)DE-He213 Hyperthermophile (dpeaa)DE-He213 Archaea (dpeaa)DE-He213 Yamashita, Keita verfasserin aut Kawazuma, Kohei verfasserin aut Kanai, Tamotsu verfasserin aut Atomi, Haruyuki verfasserin aut Imanaka, Tadayuki verfasserin aut Fujiwara, Shinsuke verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 21(2017), 5 vom: 07. Juli, Seite 903-917 (DE-627)SPR007852657 nnns volume:21 year:2017 number:5 day:07 month:07 pages:903-917 https://dx.doi.org/10.1007/s00792-017-0952-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 21 2017 5 07 07 903-917 |
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10.1007/s00792-017-0952-0 doi (DE-627)SPR007866046 (SPR)s00792-017-0952-0-e DE-627 ger DE-627 rakwb eng Hidese, Ryota verfasserin aut Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The redox-responsive regulator SurR in the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus kodakarensis binds to the SurR-binding consensus sequence (SBS) by responding to the presence of elemental sulfur. Here we constructed a surR gene disruption strain (DTS) in T. kodakarensis, and identified the genes that were under SurR control by comparing the transcriptomes of DTS and parent strains. Among these genes, transcript levels of ferredoxin:$ NADP^{+} $ oxidoreductases 1 and 2 (FNOR1 and FNOR2) genes displayed opposite responses to surR deletion, indicating that SurR repressed FNOR1 transcription while enhancing FNOR2 transcription. Each promoter region contains an SBS upstream (uSBS) and downstream (dSBS) of TATA. In addition to in vitro binding assays, we examined the roles of each SBS in vivo. In FNOR1, mutations in either one of the SBSs resulted in a complete loss of repression, indicating that the presence of both SBSs was essential for repression. In FNOR2, uSBS indeed functioned to enhance gene expression, whereas dSBS functioned in gene repression. SurR bound to uSBS2 of FNOR2 more efficiently than to dSBS2 in vitro, which may explain why SurR overall enhances FNOR2 transcription. Further analyses indicated the importance in the distance between uSBS and TATA for transcriptional activation in FNOR2. Transcriptional regulator (dpeaa)DE-He213 Redox-responsive (dpeaa)DE-He213 Regulation mechanism (dpeaa)DE-He213 Hyperthermophile (dpeaa)DE-He213 Archaea (dpeaa)DE-He213 Yamashita, Keita verfasserin aut Kawazuma, Kohei verfasserin aut Kanai, Tamotsu verfasserin aut Atomi, Haruyuki verfasserin aut Imanaka, Tadayuki verfasserin aut Fujiwara, Shinsuke verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 21(2017), 5 vom: 07. Juli, Seite 903-917 (DE-627)SPR007852657 nnns volume:21 year:2017 number:5 day:07 month:07 pages:903-917 https://dx.doi.org/10.1007/s00792-017-0952-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 21 2017 5 07 07 903-917 |
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10.1007/s00792-017-0952-0 doi (DE-627)SPR007866046 (SPR)s00792-017-0952-0-e DE-627 ger DE-627 rakwb eng Hidese, Ryota verfasserin aut Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The redox-responsive regulator SurR in the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus kodakarensis binds to the SurR-binding consensus sequence (SBS) by responding to the presence of elemental sulfur. Here we constructed a surR gene disruption strain (DTS) in T. kodakarensis, and identified the genes that were under SurR control by comparing the transcriptomes of DTS and parent strains. Among these genes, transcript levels of ferredoxin:$ NADP^{+} $ oxidoreductases 1 and 2 (FNOR1 and FNOR2) genes displayed opposite responses to surR deletion, indicating that SurR repressed FNOR1 transcription while enhancing FNOR2 transcription. Each promoter region contains an SBS upstream (uSBS) and downstream (dSBS) of TATA. In addition to in vitro binding assays, we examined the roles of each SBS in vivo. In FNOR1, mutations in either one of the SBSs resulted in a complete loss of repression, indicating that the presence of both SBSs was essential for repression. In FNOR2, uSBS indeed functioned to enhance gene expression, whereas dSBS functioned in gene repression. SurR bound to uSBS2 of FNOR2 more efficiently than to dSBS2 in vitro, which may explain why SurR overall enhances FNOR2 transcription. Further analyses indicated the importance in the distance between uSBS and TATA for transcriptional activation in FNOR2. Transcriptional regulator (dpeaa)DE-He213 Redox-responsive (dpeaa)DE-He213 Regulation mechanism (dpeaa)DE-He213 Hyperthermophile (dpeaa)DE-He213 Archaea (dpeaa)DE-He213 Yamashita, Keita verfasserin aut Kawazuma, Kohei verfasserin aut Kanai, Tamotsu verfasserin aut Atomi, Haruyuki verfasserin aut Imanaka, Tadayuki verfasserin aut Fujiwara, Shinsuke verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 21(2017), 5 vom: 07. Juli, Seite 903-917 (DE-627)SPR007852657 nnns volume:21 year:2017 number:5 day:07 month:07 pages:903-917 https://dx.doi.org/10.1007/s00792-017-0952-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 21 2017 5 07 07 903-917 |
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10.1007/s00792-017-0952-0 doi (DE-627)SPR007866046 (SPR)s00792-017-0952-0-e DE-627 ger DE-627 rakwb eng Hidese, Ryota verfasserin aut Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The redox-responsive regulator SurR in the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus kodakarensis binds to the SurR-binding consensus sequence (SBS) by responding to the presence of elemental sulfur. Here we constructed a surR gene disruption strain (DTS) in T. kodakarensis, and identified the genes that were under SurR control by comparing the transcriptomes of DTS and parent strains. Among these genes, transcript levels of ferredoxin:$ NADP^{+} $ oxidoreductases 1 and 2 (FNOR1 and FNOR2) genes displayed opposite responses to surR deletion, indicating that SurR repressed FNOR1 transcription while enhancing FNOR2 transcription. Each promoter region contains an SBS upstream (uSBS) and downstream (dSBS) of TATA. In addition to in vitro binding assays, we examined the roles of each SBS in vivo. In FNOR1, mutations in either one of the SBSs resulted in a complete loss of repression, indicating that the presence of both SBSs was essential for repression. In FNOR2, uSBS indeed functioned to enhance gene expression, whereas dSBS functioned in gene repression. SurR bound to uSBS2 of FNOR2 more efficiently than to dSBS2 in vitro, which may explain why SurR overall enhances FNOR2 transcription. Further analyses indicated the importance in the distance between uSBS and TATA for transcriptional activation in FNOR2. Transcriptional regulator (dpeaa)DE-He213 Redox-responsive (dpeaa)DE-He213 Regulation mechanism (dpeaa)DE-He213 Hyperthermophile (dpeaa)DE-He213 Archaea (dpeaa)DE-He213 Yamashita, Keita verfasserin aut Kawazuma, Kohei verfasserin aut Kanai, Tamotsu verfasserin aut Atomi, Haruyuki verfasserin aut Imanaka, Tadayuki verfasserin aut Fujiwara, Shinsuke verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 21(2017), 5 vom: 07. Juli, Seite 903-917 (DE-627)SPR007852657 nnns volume:21 year:2017 number:5 day:07 month:07 pages:903-917 https://dx.doi.org/10.1007/s00792-017-0952-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 21 2017 5 07 07 903-917 |
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10.1007/s00792-017-0952-0 doi (DE-627)SPR007866046 (SPR)s00792-017-0952-0-e DE-627 ger DE-627 rakwb eng Hidese, Ryota verfasserin aut Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The redox-responsive regulator SurR in the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus kodakarensis binds to the SurR-binding consensus sequence (SBS) by responding to the presence of elemental sulfur. Here we constructed a surR gene disruption strain (DTS) in T. kodakarensis, and identified the genes that were under SurR control by comparing the transcriptomes of DTS and parent strains. Among these genes, transcript levels of ferredoxin:$ NADP^{+} $ oxidoreductases 1 and 2 (FNOR1 and FNOR2) genes displayed opposite responses to surR deletion, indicating that SurR repressed FNOR1 transcription while enhancing FNOR2 transcription. Each promoter region contains an SBS upstream (uSBS) and downstream (dSBS) of TATA. In addition to in vitro binding assays, we examined the roles of each SBS in vivo. In FNOR1, mutations in either one of the SBSs resulted in a complete loss of repression, indicating that the presence of both SBSs was essential for repression. In FNOR2, uSBS indeed functioned to enhance gene expression, whereas dSBS functioned in gene repression. SurR bound to uSBS2 of FNOR2 more efficiently than to dSBS2 in vitro, which may explain why SurR overall enhances FNOR2 transcription. Further analyses indicated the importance in the distance between uSBS and TATA for transcriptional activation in FNOR2. Transcriptional regulator (dpeaa)DE-He213 Redox-responsive (dpeaa)DE-He213 Regulation mechanism (dpeaa)DE-He213 Hyperthermophile (dpeaa)DE-He213 Archaea (dpeaa)DE-He213 Yamashita, Keita verfasserin aut Kawazuma, Kohei verfasserin aut Kanai, Tamotsu verfasserin aut Atomi, Haruyuki verfasserin aut Imanaka, Tadayuki verfasserin aut Fujiwara, Shinsuke verfasserin aut Enthalten in Extremophiles Springer-Verlag, 2001 21(2017), 5 vom: 07. Juli, Seite 903-917 (DE-627)SPR007852657 nnns volume:21 year:2017 number:5 day:07 month:07 pages:903-917 https://dx.doi.org/10.1007/s00792-017-0952-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 21 2017 5 07 07 903-917 |
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Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis Transcriptional regulator (dpeaa)DE-He213 Redox-responsive (dpeaa)DE-He213 Regulation mechanism (dpeaa)DE-He213 Hyperthermophile (dpeaa)DE-He213 Archaea (dpeaa)DE-He213 |
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Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis |
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| title_full |
Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis |
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Hidese, Ryota |
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2017 |
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Hidese, Ryota Yamashita, Keita Kawazuma, Kohei Kanai, Tamotsu Atomi, Haruyuki Imanaka, Tadayuki Fujiwara, Shinsuke |
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Hidese, Ryota |
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10.1007/s00792-017-0952-0 |
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gene regulation of two ferredoxin:$ nadp^{+} $ oxidoreductases by the redox-responsive regulator surr in thermococcus kodakarensis |
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Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis |
| abstract |
Abstract The redox-responsive regulator SurR in the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus kodakarensis binds to the SurR-binding consensus sequence (SBS) by responding to the presence of elemental sulfur. Here we constructed a surR gene disruption strain (DTS) in T. kodakarensis, and identified the genes that were under SurR control by comparing the transcriptomes of DTS and parent strains. Among these genes, transcript levels of ferredoxin:$ NADP^{+} $ oxidoreductases 1 and 2 (FNOR1 and FNOR2) genes displayed opposite responses to surR deletion, indicating that SurR repressed FNOR1 transcription while enhancing FNOR2 transcription. Each promoter region contains an SBS upstream (uSBS) and downstream (dSBS) of TATA. In addition to in vitro binding assays, we examined the roles of each SBS in vivo. In FNOR1, mutations in either one of the SBSs resulted in a complete loss of repression, indicating that the presence of both SBSs was essential for repression. In FNOR2, uSBS indeed functioned to enhance gene expression, whereas dSBS functioned in gene repression. SurR bound to uSBS2 of FNOR2 more efficiently than to dSBS2 in vitro, which may explain why SurR overall enhances FNOR2 transcription. Further analyses indicated the importance in the distance between uSBS and TATA for transcriptional activation in FNOR2. |
| abstractGer |
Abstract The redox-responsive regulator SurR in the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus kodakarensis binds to the SurR-binding consensus sequence (SBS) by responding to the presence of elemental sulfur. Here we constructed a surR gene disruption strain (DTS) in T. kodakarensis, and identified the genes that were under SurR control by comparing the transcriptomes of DTS and parent strains. Among these genes, transcript levels of ferredoxin:$ NADP^{+} $ oxidoreductases 1 and 2 (FNOR1 and FNOR2) genes displayed opposite responses to surR deletion, indicating that SurR repressed FNOR1 transcription while enhancing FNOR2 transcription. Each promoter region contains an SBS upstream (uSBS) and downstream (dSBS) of TATA. In addition to in vitro binding assays, we examined the roles of each SBS in vivo. In FNOR1, mutations in either one of the SBSs resulted in a complete loss of repression, indicating that the presence of both SBSs was essential for repression. In FNOR2, uSBS indeed functioned to enhance gene expression, whereas dSBS functioned in gene repression. SurR bound to uSBS2 of FNOR2 more efficiently than to dSBS2 in vitro, which may explain why SurR overall enhances FNOR2 transcription. Further analyses indicated the importance in the distance between uSBS and TATA for transcriptional activation in FNOR2. |
| abstract_unstemmed |
Abstract The redox-responsive regulator SurR in the hyperthermophilic archaea Pyrococcus furiosus and Thermococcus kodakarensis binds to the SurR-binding consensus sequence (SBS) by responding to the presence of elemental sulfur. Here we constructed a surR gene disruption strain (DTS) in T. kodakarensis, and identified the genes that were under SurR control by comparing the transcriptomes of DTS and parent strains. Among these genes, transcript levels of ferredoxin:$ NADP^{+} $ oxidoreductases 1 and 2 (FNOR1 and FNOR2) genes displayed opposite responses to surR deletion, indicating that SurR repressed FNOR1 transcription while enhancing FNOR2 transcription. Each promoter region contains an SBS upstream (uSBS) and downstream (dSBS) of TATA. In addition to in vitro binding assays, we examined the roles of each SBS in vivo. In FNOR1, mutations in either one of the SBSs resulted in a complete loss of repression, indicating that the presence of both SBSs was essential for repression. In FNOR2, uSBS indeed functioned to enhance gene expression, whereas dSBS functioned in gene repression. SurR bound to uSBS2 of FNOR2 more efficiently than to dSBS2 in vitro, which may explain why SurR overall enhances FNOR2 transcription. Further analyses indicated the importance in the distance between uSBS and TATA for transcriptional activation in FNOR2. |
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Gene regulation of two ferredoxin:$ NADP^{+} $ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis |
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Yamashita, Keita Kawazuma, Kohei Kanai, Tamotsu Atomi, Haruyuki Imanaka, Tadayuki Fujiwara, Shinsuke |
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