Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion

Background Cardiopulmonary bypass (CPB) and hypothermic circulatory arrest (HCA) are important components of congenital cardiac surgery. Ischemia/reperfusion injury and inflammatory cascade activation result in endothelial damage and vascular leak, which are clinically manifested as pulmonary edema...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Tabbutt, Sarah [verfasserIn] Nelson, David P. Tsai, Nina Miura, Takuya Hickey, Paul R. Mayer, John E. Neufeld, Ellis J.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	1997

Schlagwörter:	AQP1 mRNA Levels Aquaporins (AQP1) Reperfusion Hypothermic Circulatory Arrest (HCA) Subtractive Cloning

Anmerkung:	© Picower Institute Press 1997

Übergeordnetes Werk:	Enthalten in: Molecular medicine - [London] : BioMed Central, 1994, 3(1997), 9 vom: 01. Sept., Seite 600-609
Übergeordnetes Werk:	volume:3 ; year:1997 ; number:9 ; day:01 ; month:09 ; pages:600-609

Links:	Volltext

DOI / URN:	10.1007/BF03401817

Katalog-ID:	SPR008055335

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520			\|a Background Cardiopulmonary bypass (CPB) and hypothermic circulatory arrest (HCA) are important components of congenital cardiac surgery. Ischemia/reperfusion injury and inflammatory cascade activation result in endothelial damage and vascular leak, which are clinically manifested as pulmonary edema and low cardiac output postoperatively. Newborns are particularly susceptible. Subtraction cloning is a useful method of isolating induced genes and can be applied to CPB/HCA. Materials and Methods We used a newborn lamb model replicating infant CPB with HCA to obtain tissues during various periods of reperfusion. We utilized subtraction cloning to identify mRNA induced in lung following CPB/HCA and reperfusion. Ribonuclease protection was used to quantify mRNA levels. Results We isolated a cDNA encoding ovine aquaporin-1 in a subtracted cDNA screen comparing control lung with lung exposed to CPB/HCA and reperfusion. Aquaporin-1 mRNA levels increased 3-fold in lung (p =.006) exposed to CPB/HCA and 6 hr of reperfusion. No induction was observed immediately following bypass or after 3 hr of reperfusion. We found no significant induction of aquaporin-1 mRNA following bypass, arrest, and reperfusion in other tissues surveyed, including ventricle, atrium, skeletal muscle, kidney, brain, and liver. Conclusions Our finding that aquaporin-1 mRNA is reproducibly induced in lung following CPB/HCA with 6 hr of reperfusion suggests an important role for the water channel in the setting of pulmonary edema. Induction of Aquaporin-1 is late compared with other inflammatory mediators (ICAM-1, E-selectin, IL-8). Further studies are needed to determine if aquaporin-1 contributes to the disease process or if it is part of the recovery phase.
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700	1		\|a Neufeld, Ellis J. \|4 aut
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allfields	10.1007/BF03401817 doi (DE-627)SPR008055335 (SPR)BF03401817-e DE-627 ger DE-627 rakwb eng Tabbutt, Sarah verfasserin aut Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion 1997 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Picower Institute Press 1997 Background Cardiopulmonary bypass (CPB) and hypothermic circulatory arrest (HCA) are important components of congenital cardiac surgery. Ischemia/reperfusion injury and inflammatory cascade activation result in endothelial damage and vascular leak, which are clinically manifested as pulmonary edema and low cardiac output postoperatively. Newborns are particularly susceptible. Subtraction cloning is a useful method of isolating induced genes and can be applied to CPB/HCA. Materials and Methods We used a newborn lamb model replicating infant CPB with HCA to obtain tissues during various periods of reperfusion. We utilized subtraction cloning to identify mRNA induced in lung following CPB/HCA and reperfusion. Ribonuclease protection was used to quantify mRNA levels. Results We isolated a cDNA encoding ovine aquaporin-1 in a subtracted cDNA screen comparing control lung with lung exposed to CPB/HCA and reperfusion. Aquaporin-1 mRNA levels increased 3-fold in lung (p =.006) exposed to CPB/HCA and 6 hr of reperfusion. No induction was observed immediately following bypass or after 3 hr of reperfusion. We found no significant induction of aquaporin-1 mRNA following bypass, arrest, and reperfusion in other tissues surveyed, including ventricle, atrium, skeletal muscle, kidney, brain, and liver. Conclusions Our finding that aquaporin-1 mRNA is reproducibly induced in lung following CPB/HCA with 6 hr of reperfusion suggests an important role for the water channel in the setting of pulmonary edema. Induction of Aquaporin-1 is late compared with other inflammatory mediators (ICAM-1, E-selectin, IL-8). Further studies are needed to determine if aquaporin-1 contributes to the disease process or if it is part of the recovery phase. AQP1 mRNA Levels (dpeaa)DE-He213 Aquaporins (AQP1) (dpeaa)DE-He213 Reperfusion (dpeaa)DE-He213 Hypothermic Circulatory Arrest (HCA) (dpeaa)DE-He213 Subtractive Cloning (dpeaa)DE-He213 Nelson, David P. aut Tsai, Nina aut Miura, Takuya aut Hickey, Paul R. aut Mayer, John E. aut Neufeld, Ellis J. aut Enthalten in Molecular medicine [London] : BioMed Central, 1994 3(1997), 9 vom: 01. Sept., Seite 600-609 (DE-627)269539611 (DE-600)1475577-4 1528-3658 nnns volume:3 year:1997 number:9 day:01 month:09 pages:600-609 https://dx.doi.org/10.1007/BF03401817 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 1997 9 01 09 600-609
spelling	10.1007/BF03401817 doi (DE-627)SPR008055335 (SPR)BF03401817-e DE-627 ger DE-627 rakwb eng Tabbutt, Sarah verfasserin aut Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion 1997 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Picower Institute Press 1997 Background Cardiopulmonary bypass (CPB) and hypothermic circulatory arrest (HCA) are important components of congenital cardiac surgery. Ischemia/reperfusion injury and inflammatory cascade activation result in endothelial damage and vascular leak, which are clinically manifested as pulmonary edema and low cardiac output postoperatively. Newborns are particularly susceptible. Subtraction cloning is a useful method of isolating induced genes and can be applied to CPB/HCA. Materials and Methods We used a newborn lamb model replicating infant CPB with HCA to obtain tissues during various periods of reperfusion. We utilized subtraction cloning to identify mRNA induced in lung following CPB/HCA and reperfusion. Ribonuclease protection was used to quantify mRNA levels. Results We isolated a cDNA encoding ovine aquaporin-1 in a subtracted cDNA screen comparing control lung with lung exposed to CPB/HCA and reperfusion. Aquaporin-1 mRNA levels increased 3-fold in lung (p =.006) exposed to CPB/HCA and 6 hr of reperfusion. No induction was observed immediately following bypass or after 3 hr of reperfusion. We found no significant induction of aquaporin-1 mRNA following bypass, arrest, and reperfusion in other tissues surveyed, including ventricle, atrium, skeletal muscle, kidney, brain, and liver. Conclusions Our finding that aquaporin-1 mRNA is reproducibly induced in lung following CPB/HCA with 6 hr of reperfusion suggests an important role for the water channel in the setting of pulmonary edema. Induction of Aquaporin-1 is late compared with other inflammatory mediators (ICAM-1, E-selectin, IL-8). Further studies are needed to determine if aquaporin-1 contributes to the disease process or if it is part of the recovery phase. AQP1 mRNA Levels (dpeaa)DE-He213 Aquaporins (AQP1) (dpeaa)DE-He213 Reperfusion (dpeaa)DE-He213 Hypothermic Circulatory Arrest (HCA) (dpeaa)DE-He213 Subtractive Cloning (dpeaa)DE-He213 Nelson, David P. aut Tsai, Nina aut Miura, Takuya aut Hickey, Paul R. aut Mayer, John E. aut Neufeld, Ellis J. aut Enthalten in Molecular medicine [London] : BioMed Central, 1994 3(1997), 9 vom: 01. Sept., Seite 600-609 (DE-627)269539611 (DE-600)1475577-4 1528-3658 nnns volume:3 year:1997 number:9 day:01 month:09 pages:600-609 https://dx.doi.org/10.1007/BF03401817 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 1997 9 01 09 600-609
allfields_unstemmed	10.1007/BF03401817 doi (DE-627)SPR008055335 (SPR)BF03401817-e DE-627 ger DE-627 rakwb eng Tabbutt, Sarah verfasserin aut Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion 1997 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Picower Institute Press 1997 Background Cardiopulmonary bypass (CPB) and hypothermic circulatory arrest (HCA) are important components of congenital cardiac surgery. Ischemia/reperfusion injury and inflammatory cascade activation result in endothelial damage and vascular leak, which are clinically manifested as pulmonary edema and low cardiac output postoperatively. Newborns are particularly susceptible. Subtraction cloning is a useful method of isolating induced genes and can be applied to CPB/HCA. Materials and Methods We used a newborn lamb model replicating infant CPB with HCA to obtain tissues during various periods of reperfusion. We utilized subtraction cloning to identify mRNA induced in lung following CPB/HCA and reperfusion. Ribonuclease protection was used to quantify mRNA levels. Results We isolated a cDNA encoding ovine aquaporin-1 in a subtracted cDNA screen comparing control lung with lung exposed to CPB/HCA and reperfusion. Aquaporin-1 mRNA levels increased 3-fold in lung (p =.006) exposed to CPB/HCA and 6 hr of reperfusion. No induction was observed immediately following bypass or after 3 hr of reperfusion. We found no significant induction of aquaporin-1 mRNA following bypass, arrest, and reperfusion in other tissues surveyed, including ventricle, atrium, skeletal muscle, kidney, brain, and liver. Conclusions Our finding that aquaporin-1 mRNA is reproducibly induced in lung following CPB/HCA with 6 hr of reperfusion suggests an important role for the water channel in the setting of pulmonary edema. Induction of Aquaporin-1 is late compared with other inflammatory mediators (ICAM-1, E-selectin, IL-8). Further studies are needed to determine if aquaporin-1 contributes to the disease process or if it is part of the recovery phase. AQP1 mRNA Levels (dpeaa)DE-He213 Aquaporins (AQP1) (dpeaa)DE-He213 Reperfusion (dpeaa)DE-He213 Hypothermic Circulatory Arrest (HCA) (dpeaa)DE-He213 Subtractive Cloning (dpeaa)DE-He213 Nelson, David P. aut Tsai, Nina aut Miura, Takuya aut Hickey, Paul R. aut Mayer, John E. aut Neufeld, Ellis J. aut Enthalten in Molecular medicine [London] : BioMed Central, 1994 3(1997), 9 vom: 01. Sept., Seite 600-609 (DE-627)269539611 (DE-600)1475577-4 1528-3658 nnns volume:3 year:1997 number:9 day:01 month:09 pages:600-609 https://dx.doi.org/10.1007/BF03401817 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 1997 9 01 09 600-609
allfieldsGer	10.1007/BF03401817 doi (DE-627)SPR008055335 (SPR)BF03401817-e DE-627 ger DE-627 rakwb eng Tabbutt, Sarah verfasserin aut Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion 1997 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Picower Institute Press 1997 Background Cardiopulmonary bypass (CPB) and hypothermic circulatory arrest (HCA) are important components of congenital cardiac surgery. Ischemia/reperfusion injury and inflammatory cascade activation result in endothelial damage and vascular leak, which are clinically manifested as pulmonary edema and low cardiac output postoperatively. Newborns are particularly susceptible. Subtraction cloning is a useful method of isolating induced genes and can be applied to CPB/HCA. Materials and Methods We used a newborn lamb model replicating infant CPB with HCA to obtain tissues during various periods of reperfusion. We utilized subtraction cloning to identify mRNA induced in lung following CPB/HCA and reperfusion. Ribonuclease protection was used to quantify mRNA levels. Results We isolated a cDNA encoding ovine aquaporin-1 in a subtracted cDNA screen comparing control lung with lung exposed to CPB/HCA and reperfusion. Aquaporin-1 mRNA levels increased 3-fold in lung (p =.006) exposed to CPB/HCA and 6 hr of reperfusion. No induction was observed immediately following bypass or after 3 hr of reperfusion. We found no significant induction of aquaporin-1 mRNA following bypass, arrest, and reperfusion in other tissues surveyed, including ventricle, atrium, skeletal muscle, kidney, brain, and liver. Conclusions Our finding that aquaporin-1 mRNA is reproducibly induced in lung following CPB/HCA with 6 hr of reperfusion suggests an important role for the water channel in the setting of pulmonary edema. Induction of Aquaporin-1 is late compared with other inflammatory mediators (ICAM-1, E-selectin, IL-8). Further studies are needed to determine if aquaporin-1 contributes to the disease process or if it is part of the recovery phase. AQP1 mRNA Levels (dpeaa)DE-He213 Aquaporins (AQP1) (dpeaa)DE-He213 Reperfusion (dpeaa)DE-He213 Hypothermic Circulatory Arrest (HCA) (dpeaa)DE-He213 Subtractive Cloning (dpeaa)DE-He213 Nelson, David P. aut Tsai, Nina aut Miura, Takuya aut Hickey, Paul R. aut Mayer, John E. aut Neufeld, Ellis J. aut Enthalten in Molecular medicine [London] : BioMed Central, 1994 3(1997), 9 vom: 01. Sept., Seite 600-609 (DE-627)269539611 (DE-600)1475577-4 1528-3658 nnns volume:3 year:1997 number:9 day:01 month:09 pages:600-609 https://dx.doi.org/10.1007/BF03401817 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 1997 9 01 09 600-609
allfieldsSound	10.1007/BF03401817 doi (DE-627)SPR008055335 (SPR)BF03401817-e DE-627 ger DE-627 rakwb eng Tabbutt, Sarah verfasserin aut Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion 1997 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Picower Institute Press 1997 Background Cardiopulmonary bypass (CPB) and hypothermic circulatory arrest (HCA) are important components of congenital cardiac surgery. Ischemia/reperfusion injury and inflammatory cascade activation result in endothelial damage and vascular leak, which are clinically manifested as pulmonary edema and low cardiac output postoperatively. Newborns are particularly susceptible. Subtraction cloning is a useful method of isolating induced genes and can be applied to CPB/HCA. Materials and Methods We used a newborn lamb model replicating infant CPB with HCA to obtain tissues during various periods of reperfusion. We utilized subtraction cloning to identify mRNA induced in lung following CPB/HCA and reperfusion. Ribonuclease protection was used to quantify mRNA levels. Results We isolated a cDNA encoding ovine aquaporin-1 in a subtracted cDNA screen comparing control lung with lung exposed to CPB/HCA and reperfusion. Aquaporin-1 mRNA levels increased 3-fold in lung (p =.006) exposed to CPB/HCA and 6 hr of reperfusion. No induction was observed immediately following bypass or after 3 hr of reperfusion. We found no significant induction of aquaporin-1 mRNA following bypass, arrest, and reperfusion in other tissues surveyed, including ventricle, atrium, skeletal muscle, kidney, brain, and liver. Conclusions Our finding that aquaporin-1 mRNA is reproducibly induced in lung following CPB/HCA with 6 hr of reperfusion suggests an important role for the water channel in the setting of pulmonary edema. Induction of Aquaporin-1 is late compared with other inflammatory mediators (ICAM-1, E-selectin, IL-8). Further studies are needed to determine if aquaporin-1 contributes to the disease process or if it is part of the recovery phase. AQP1 mRNA Levels (dpeaa)DE-He213 Aquaporins (AQP1) (dpeaa)DE-He213 Reperfusion (dpeaa)DE-He213 Hypothermic Circulatory Arrest (HCA) (dpeaa)DE-He213 Subtractive Cloning (dpeaa)DE-He213 Nelson, David P. aut Tsai, Nina aut Miura, Takuya aut Hickey, Paul R. aut Mayer, John E. aut Neufeld, Ellis J. aut Enthalten in Molecular medicine [London] : BioMed Central, 1994 3(1997), 9 vom: 01. Sept., Seite 600-609 (DE-627)269539611 (DE-600)1475577-4 1528-3658 nnns volume:3 year:1997 number:9 day:01 month:09 pages:600-609 https://dx.doi.org/10.1007/BF03401817 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 1997 9 01 09 600-609
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author	Tabbutt, Sarah
spellingShingle	Tabbutt, Sarah misc AQP1 mRNA Levels misc Aquaporins (AQP1) misc Reperfusion misc Hypothermic Circulatory Arrest (HCA) misc Subtractive Cloning Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion
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topic_title	Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion AQP1 mRNA Levels (dpeaa)DE-He213 Aquaporins (AQP1) (dpeaa)DE-He213 Reperfusion (dpeaa)DE-He213 Hypothermic Circulatory Arrest (HCA) (dpeaa)DE-He213 Subtractive Cloning (dpeaa)DE-He213
topic	misc AQP1 mRNA Levels misc Aquaporins (AQP1) misc Reperfusion misc Hypothermic Circulatory Arrest (HCA) misc Subtractive Cloning
topic_unstemmed	misc AQP1 mRNA Levels misc Aquaporins (AQP1) misc Reperfusion misc Hypothermic Circulatory Arrest (HCA) misc Subtractive Cloning
topic_browse	misc AQP1 mRNA Levels misc Aquaporins (AQP1) misc Reperfusion misc Hypothermic Circulatory Arrest (HCA) misc Subtractive Cloning
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title	Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion
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title_full	Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion
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author_browse	Tabbutt, Sarah Nelson, David P. Tsai, Nina Miura, Takuya Hickey, Paul R. Mayer, John E. Neufeld, Ellis J.
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title_sort	induction of aquaporin-1 mrna following cardiopulmonary bypass and reperfusion
title_auth	Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion
abstract	Background Cardiopulmonary bypass (CPB) and hypothermic circulatory arrest (HCA) are important components of congenital cardiac surgery. Ischemia/reperfusion injury and inflammatory cascade activation result in endothelial damage and vascular leak, which are clinically manifested as pulmonary edema and low cardiac output postoperatively. Newborns are particularly susceptible. Subtraction cloning is a useful method of isolating induced genes and can be applied to CPB/HCA. Materials and Methods We used a newborn lamb model replicating infant CPB with HCA to obtain tissues during various periods of reperfusion. We utilized subtraction cloning to identify mRNA induced in lung following CPB/HCA and reperfusion. Ribonuclease protection was used to quantify mRNA levels. Results We isolated a cDNA encoding ovine aquaporin-1 in a subtracted cDNA screen comparing control lung with lung exposed to CPB/HCA and reperfusion. Aquaporin-1 mRNA levels increased 3-fold in lung (p =.006) exposed to CPB/HCA and 6 hr of reperfusion. No induction was observed immediately following bypass or after 3 hr of reperfusion. We found no significant induction of aquaporin-1 mRNA following bypass, arrest, and reperfusion in other tissues surveyed, including ventricle, atrium, skeletal muscle, kidney, brain, and liver. Conclusions Our finding that aquaporin-1 mRNA is reproducibly induced in lung following CPB/HCA with 6 hr of reperfusion suggests an important role for the water channel in the setting of pulmonary edema. Induction of Aquaporin-1 is late compared with other inflammatory mediators (ICAM-1, E-selectin, IL-8). Further studies are needed to determine if aquaporin-1 contributes to the disease process or if it is part of the recovery phase. © Picower Institute Press 1997
abstractGer	Background Cardiopulmonary bypass (CPB) and hypothermic circulatory arrest (HCA) are important components of congenital cardiac surgery. Ischemia/reperfusion injury and inflammatory cascade activation result in endothelial damage and vascular leak, which are clinically manifested as pulmonary edema and low cardiac output postoperatively. Newborns are particularly susceptible. Subtraction cloning is a useful method of isolating induced genes and can be applied to CPB/HCA. Materials and Methods We used a newborn lamb model replicating infant CPB with HCA to obtain tissues during various periods of reperfusion. We utilized subtraction cloning to identify mRNA induced in lung following CPB/HCA and reperfusion. Ribonuclease protection was used to quantify mRNA levels. Results We isolated a cDNA encoding ovine aquaporin-1 in a subtracted cDNA screen comparing control lung with lung exposed to CPB/HCA and reperfusion. Aquaporin-1 mRNA levels increased 3-fold in lung (p =.006) exposed to CPB/HCA and 6 hr of reperfusion. No induction was observed immediately following bypass or after 3 hr of reperfusion. We found no significant induction of aquaporin-1 mRNA following bypass, arrest, and reperfusion in other tissues surveyed, including ventricle, atrium, skeletal muscle, kidney, brain, and liver. Conclusions Our finding that aquaporin-1 mRNA is reproducibly induced in lung following CPB/HCA with 6 hr of reperfusion suggests an important role for the water channel in the setting of pulmonary edema. Induction of Aquaporin-1 is late compared with other inflammatory mediators (ICAM-1, E-selectin, IL-8). Further studies are needed to determine if aquaporin-1 contributes to the disease process or if it is part of the recovery phase. © Picower Institute Press 1997
abstract_unstemmed	Background Cardiopulmonary bypass (CPB) and hypothermic circulatory arrest (HCA) are important components of congenital cardiac surgery. Ischemia/reperfusion injury and inflammatory cascade activation result in endothelial damage and vascular leak, which are clinically manifested as pulmonary edema and low cardiac output postoperatively. Newborns are particularly susceptible. Subtraction cloning is a useful method of isolating induced genes and can be applied to CPB/HCA. Materials and Methods We used a newborn lamb model replicating infant CPB with HCA to obtain tissues during various periods of reperfusion. We utilized subtraction cloning to identify mRNA induced in lung following CPB/HCA and reperfusion. Ribonuclease protection was used to quantify mRNA levels. Results We isolated a cDNA encoding ovine aquaporin-1 in a subtracted cDNA screen comparing control lung with lung exposed to CPB/HCA and reperfusion. Aquaporin-1 mRNA levels increased 3-fold in lung (p =.006) exposed to CPB/HCA and 6 hr of reperfusion. No induction was observed immediately following bypass or after 3 hr of reperfusion. We found no significant induction of aquaporin-1 mRNA following bypass, arrest, and reperfusion in other tissues surveyed, including ventricle, atrium, skeletal muscle, kidney, brain, and liver. Conclusions Our finding that aquaporin-1 mRNA is reproducibly induced in lung following CPB/HCA with 6 hr of reperfusion suggests an important role for the water channel in the setting of pulmonary edema. Induction of Aquaporin-1 is late compared with other inflammatory mediators (ICAM-1, E-selectin, IL-8). Further studies are needed to determine if aquaporin-1 contributes to the disease process or if it is part of the recovery phase. © Picower Institute Press 1997
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title_short	Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion
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author2	Nelson, David P. Tsai, Nina Miura, Takuya Hickey, Paul R. Mayer, John E. Neufeld, Ellis J.
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We found no significant induction of aquaporin-1 mRNA following bypass, arrest, and reperfusion in other tissues surveyed, including ventricle, atrium, skeletal muscle, kidney, brain, and liver. Conclusions Our finding that aquaporin-1 mRNA is reproducibly induced in lung following CPB/HCA with 6 hr of reperfusion suggests an important role for the water channel in the setting of pulmonary edema. Induction of Aquaporin-1 is late compared with other inflammatory mediators (ICAM-1, E-selectin, IL-8). 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Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion

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