Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System
Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen proc...
Ausführliche Beschreibung
Autor*in: |
Manterola, J. M. [verfasserIn] Thornton, C. G. [verfasserIn] Padilla, E. [verfasserIn] Lonca, J. [verfasserIn] Corea, I. [verfasserIn] Martínez, E. [verfasserIn] Ausina, V. [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2003 |
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Schlagwörter: |
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Übergeordnetes Werk: |
Enthalten in: European journal of clinical microbiology & infectious diseases - Berlin : Springer, 1982, 22(2003), 1 vom: Jan., Seite 35-42 |
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Übergeordnetes Werk: |
volume:22 ; year:2003 ; number:1 ; month:01 ; pages:35-42 |
Links: |
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DOI / URN: |
10.1007/s10096-002-0853-7 |
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Katalog-ID: |
SPR008664315 |
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100 | 1 | |a Manterola, J. M. |e verfasserin |4 aut | |
245 | 1 | 0 | |a Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System |
264 | 1 | |c 2003 | |
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520 | |a Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses $ C_{18} $-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with $ C_{18} $-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMérieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and $ C_{18} $-carboxypropylbetaine was 61.2% and 58.6% (P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% (P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2±5.6 and 15.0±8.8 days (P>0.05), respectively, and 20.0±7.6 and 15.7±8.9 days (P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. $ C_{18} $-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture. | ||
650 | 4 | |a Culture Sensitivity |7 (dpeaa)DE-He213 | |
650 | 4 | |a Contamination Rate |7 (dpeaa)DE-He213 | |
650 | 4 | |a Specimen Processing |7 (dpeaa)DE-He213 | |
650 | 4 | |a Mycobacterium Fortuitum |7 (dpeaa)DE-He213 | |
650 | 4 | |a Liquid Culture System |7 (dpeaa)DE-He213 | |
700 | 1 | |a Thornton, C. G. |e verfasserin |4 aut | |
700 | 1 | |a Padilla, E. |e verfasserin |4 aut | |
700 | 1 | |a Lonca, J. |e verfasserin |4 aut | |
700 | 1 | |a Corea, I. |e verfasserin |4 aut | |
700 | 1 | |a Martínez, E. |e verfasserin |4 aut | |
700 | 1 | |a Ausina, V. |e verfasserin |4 aut | |
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2003 |
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10.1007/s10096-002-0853-7 doi (DE-627)SPR008664315 (SPR)s10096-002-0853-7-e DE-627 ger DE-627 rakwb eng 610 ASE 44.43 bkl 44.75 bkl Manterola, J. M. verfasserin aut Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses $ C_{18} $-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with $ C_{18} $-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMérieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and $ C_{18} $-carboxypropylbetaine was 61.2% and 58.6% (P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% (P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2±5.6 and 15.0±8.8 days (P>0.05), respectively, and 20.0±7.6 and 15.7±8.9 days (P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. $ C_{18} $-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture. Culture Sensitivity (dpeaa)DE-He213 Contamination Rate (dpeaa)DE-He213 Specimen Processing (dpeaa)DE-He213 Mycobacterium Fortuitum (dpeaa)DE-He213 Liquid Culture System (dpeaa)DE-He213 Thornton, C. G. verfasserin aut Padilla, E. verfasserin aut Lonca, J. verfasserin aut Corea, I. verfasserin aut Martínez, E. verfasserin aut Ausina, V. verfasserin aut Enthalten in European journal of clinical microbiology & infectious diseases Berlin : Springer, 1982 22(2003), 1 vom: Jan., Seite 35-42 (DE-627)25372273X (DE-600)1459049-9 1435-4373 nnns volume:22 year:2003 number:1 month:01 pages:35-42 https://dx.doi.org/10.1007/s10096-002-0853-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.43 ASE 44.75 ASE AR 22 2003 1 01 35-42 |
spelling |
10.1007/s10096-002-0853-7 doi (DE-627)SPR008664315 (SPR)s10096-002-0853-7-e DE-627 ger DE-627 rakwb eng 610 ASE 44.43 bkl 44.75 bkl Manterola, J. M. verfasserin aut Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses $ C_{18} $-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with $ C_{18} $-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMérieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and $ C_{18} $-carboxypropylbetaine was 61.2% and 58.6% (P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% (P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2±5.6 and 15.0±8.8 days (P>0.05), respectively, and 20.0±7.6 and 15.7±8.9 days (P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. $ C_{18} $-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture. Culture Sensitivity (dpeaa)DE-He213 Contamination Rate (dpeaa)DE-He213 Specimen Processing (dpeaa)DE-He213 Mycobacterium Fortuitum (dpeaa)DE-He213 Liquid Culture System (dpeaa)DE-He213 Thornton, C. G. verfasserin aut Padilla, E. verfasserin aut Lonca, J. verfasserin aut Corea, I. verfasserin aut Martínez, E. verfasserin aut Ausina, V. verfasserin aut Enthalten in European journal of clinical microbiology & infectious diseases Berlin : Springer, 1982 22(2003), 1 vom: Jan., Seite 35-42 (DE-627)25372273X (DE-600)1459049-9 1435-4373 nnns volume:22 year:2003 number:1 month:01 pages:35-42 https://dx.doi.org/10.1007/s10096-002-0853-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.43 ASE 44.75 ASE AR 22 2003 1 01 35-42 |
allfields_unstemmed |
10.1007/s10096-002-0853-7 doi (DE-627)SPR008664315 (SPR)s10096-002-0853-7-e DE-627 ger DE-627 rakwb eng 610 ASE 44.43 bkl 44.75 bkl Manterola, J. M. verfasserin aut Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses $ C_{18} $-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with $ C_{18} $-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMérieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and $ C_{18} $-carboxypropylbetaine was 61.2% and 58.6% (P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% (P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2±5.6 and 15.0±8.8 days (P>0.05), respectively, and 20.0±7.6 and 15.7±8.9 days (P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. $ C_{18} $-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture. Culture Sensitivity (dpeaa)DE-He213 Contamination Rate (dpeaa)DE-He213 Specimen Processing (dpeaa)DE-He213 Mycobacterium Fortuitum (dpeaa)DE-He213 Liquid Culture System (dpeaa)DE-He213 Thornton, C. G. verfasserin aut Padilla, E. verfasserin aut Lonca, J. verfasserin aut Corea, I. verfasserin aut Martínez, E. verfasserin aut Ausina, V. verfasserin aut Enthalten in European journal of clinical microbiology & infectious diseases Berlin : Springer, 1982 22(2003), 1 vom: Jan., Seite 35-42 (DE-627)25372273X (DE-600)1459049-9 1435-4373 nnns volume:22 year:2003 number:1 month:01 pages:35-42 https://dx.doi.org/10.1007/s10096-002-0853-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.43 ASE 44.75 ASE AR 22 2003 1 01 35-42 |
allfieldsGer |
10.1007/s10096-002-0853-7 doi (DE-627)SPR008664315 (SPR)s10096-002-0853-7-e DE-627 ger DE-627 rakwb eng 610 ASE 44.43 bkl 44.75 bkl Manterola, J. M. verfasserin aut Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses $ C_{18} $-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with $ C_{18} $-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMérieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and $ C_{18} $-carboxypropylbetaine was 61.2% and 58.6% (P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% (P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2±5.6 and 15.0±8.8 days (P>0.05), respectively, and 20.0±7.6 and 15.7±8.9 days (P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. $ C_{18} $-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture. Culture Sensitivity (dpeaa)DE-He213 Contamination Rate (dpeaa)DE-He213 Specimen Processing (dpeaa)DE-He213 Mycobacterium Fortuitum (dpeaa)DE-He213 Liquid Culture System (dpeaa)DE-He213 Thornton, C. G. verfasserin aut Padilla, E. verfasserin aut Lonca, J. verfasserin aut Corea, I. verfasserin aut Martínez, E. verfasserin aut Ausina, V. verfasserin aut Enthalten in European journal of clinical microbiology & infectious diseases Berlin : Springer, 1982 22(2003), 1 vom: Jan., Seite 35-42 (DE-627)25372273X (DE-600)1459049-9 1435-4373 nnns volume:22 year:2003 number:1 month:01 pages:35-42 https://dx.doi.org/10.1007/s10096-002-0853-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.43 ASE 44.75 ASE AR 22 2003 1 01 35-42 |
allfieldsSound |
10.1007/s10096-002-0853-7 doi (DE-627)SPR008664315 (SPR)s10096-002-0853-7-e DE-627 ger DE-627 rakwb eng 610 ASE 44.43 bkl 44.75 bkl Manterola, J. M. verfasserin aut Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses $ C_{18} $-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with $ C_{18} $-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMérieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and $ C_{18} $-carboxypropylbetaine was 61.2% and 58.6% (P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% (P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2±5.6 and 15.0±8.8 days (P>0.05), respectively, and 20.0±7.6 and 15.7±8.9 days (P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. $ C_{18} $-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture. Culture Sensitivity (dpeaa)DE-He213 Contamination Rate (dpeaa)DE-He213 Specimen Processing (dpeaa)DE-He213 Mycobacterium Fortuitum (dpeaa)DE-He213 Liquid Culture System (dpeaa)DE-He213 Thornton, C. G. verfasserin aut Padilla, E. verfasserin aut Lonca, J. verfasserin aut Corea, I. verfasserin aut Martínez, E. verfasserin aut Ausina, V. verfasserin aut Enthalten in European journal of clinical microbiology & infectious diseases Berlin : Springer, 1982 22(2003), 1 vom: Jan., Seite 35-42 (DE-627)25372273X (DE-600)1459049-9 1435-4373 nnns volume:22 year:2003 number:1 month:01 pages:35-42 https://dx.doi.org/10.1007/s10096-002-0853-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.43 ASE 44.75 ASE AR 22 2003 1 01 35-42 |
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Enthalten in European journal of clinical microbiology & infectious diseases 22(2003), 1 vom: Jan., Seite 35-42 volume:22 year:2003 number:1 month:01 pages:35-42 |
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Enthalten in European journal of clinical microbiology & infectious diseases 22(2003), 1 vom: Jan., Seite 35-42 volume:22 year:2003 number:1 month:01 pages:35-42 |
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Culture Sensitivity Contamination Rate Specimen Processing Mycobacterium Fortuitum Liquid Culture System |
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European journal of clinical microbiology & infectious diseases |
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Manterola, J. M. @@aut@@ Thornton, C. G. @@aut@@ Padilla, E. @@aut@@ Lonca, J. @@aut@@ Corea, I. @@aut@@ Martínez, E. @@aut@@ Ausina, V. @@aut@@ |
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M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2003</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses $ C_{18} $-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with $ C_{18} $-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMérieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and $ C_{18} $-carboxypropylbetaine was 61.2% and 58.6% (P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% (P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2±5.6 and 15.0±8.8 days (P>0.05), respectively, and 20.0±7.6 and 15.7±8.9 days (P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. $ C_{18} $-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Culture Sensitivity</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Contamination Rate</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Specimen Processing</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mycobacterium Fortuitum</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Liquid Culture System</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Thornton, C. 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author |
Manterola, J. M. |
spellingShingle |
Manterola, J. M. ddc 610 bkl 44.43 bkl 44.75 misc Culture Sensitivity misc Contamination Rate misc Specimen Processing misc Mycobacterium Fortuitum misc Liquid Culture System Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System |
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topic_title |
610 ASE 44.43 bkl 44.75 bkl Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System Culture Sensitivity (dpeaa)DE-He213 Contamination Rate (dpeaa)DE-He213 Specimen Processing (dpeaa)DE-He213 Mycobacterium Fortuitum (dpeaa)DE-He213 Liquid Culture System (dpeaa)DE-He213 |
topic |
ddc 610 bkl 44.43 bkl 44.75 misc Culture Sensitivity misc Contamination Rate misc Specimen Processing misc Mycobacterium Fortuitum misc Liquid Culture System |
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ddc 610 bkl 44.43 bkl 44.75 misc Culture Sensitivity misc Contamination Rate misc Specimen Processing misc Mycobacterium Fortuitum misc Liquid Culture System |
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Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System |
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title_full |
Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System |
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Manterola, J. M. |
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European journal of clinical microbiology & infectious diseases |
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European journal of clinical microbiology & infectious diseases |
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600 - Technology |
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Manterola, J. M. Thornton, C. G. Padilla, E. Lonca, J. Corea, I. Martínez, E. Ausina, V. |
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Manterola, J. M. |
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10.1007/s10096-002-0853-7 |
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610 |
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verfasserin |
title_sort |
comparison of the sodium dodecyl sulfate-sodium hydroxide specimen processing method with the $ c_{18} $-carboxypropylbetaine specimen processing method using the mb/bact liquid culture system |
title_auth |
Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System |
abstract |
Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses $ C_{18} $-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with $ C_{18} $-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMérieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and $ C_{18} $-carboxypropylbetaine was 61.2% and 58.6% (P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% (P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2±5.6 and 15.0±8.8 days (P>0.05), respectively, and 20.0±7.6 and 15.7±8.9 days (P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. $ C_{18} $-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture. |
abstractGer |
Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses $ C_{18} $-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with $ C_{18} $-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMérieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and $ C_{18} $-carboxypropylbetaine was 61.2% and 58.6% (P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% (P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2±5.6 and 15.0±8.8 days (P>0.05), respectively, and 20.0±7.6 and 15.7±8.9 days (P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. $ C_{18} $-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture. |
abstract_unstemmed |
Abstract The ability of physicians to diagnose tuberculosis is impacted by the use of smear and culture techniques combined with specimen processing methods. The objective of this study was to evaluate the effects of specimen processing on smear and culture sensitivity by comparing the specimen processing method that uses $ C_{18} $-carboxypropylbetaine with the method that combines sodium dodecyl sulfate and sodium hydroxide. A total of 1,201 specimens were entered into this study. Specimens were split approximately equally such that one-half of each specimen was processed with sodium dodecyl sulfate-sodium hydroxide, while the other half was processed with $ C_{18} $-carboxypropylbetaine. All sediments were subjected to acid-fast staining and then analyzed using the MB/BacT liquid culture system (bioMérieux, France) and solid media. The sensitivity of smear following processing with sodium dodecyl sulfate-sodium hydroxide and $ C_{18} $-carboxypropylbetaine was 61.2% and 58.6% (P>0.05), respectively, while the specificities were identical (99.7%). The sensitivity of culture was 84.2% and 96.1% (P<0.05), respectively. The time to detection in the MB/BacT liquid culture system was 13.2±5.6 and 15.0±8.8 days (P>0.05), respectively, and 20.0±7.6 and 15.7±8.9 days (P<0.05), respectively, on solid media. The contamination rates in the MB/BacT system were 0.8% and 8.7%, respectively, whereas the contamination rates on solid media were 2.6% and 4.3%, respectively. $ C_{18} $-carboxypropylbetaine specimen processing was less labor-intensive than sodium dodecyl sulfate-sodium hydroxide processing and improved the ability of laboratory staff to detect the presence of mycobacteria by culture. |
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title_short |
Comparison of the Sodium Dodecyl Sulfate-Sodium Hydroxide Specimen Processing Method with the $ C_{18} $-Carboxypropylbetaine Specimen Processing Method Using the MB/BacT Liquid Culture System |
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https://dx.doi.org/10.1007/s10096-002-0853-7 |
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Thornton, C. G. Padilla, E. Lonca, J. Corea, I. Martínez, E. Ausina, V. |
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score |
7.3985567 |