Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS
Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been descr...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Veron, L. [verfasserIn] Mailler, S. [verfasserIn] Girard, V. [verfasserIn] Muller, B. H. [verfasserIn] L’Hostis, G. [verfasserIn] Ducruix, C. [verfasserIn] Lesenne, A. [verfasserIn] Richez, A. [verfasserIn] Rostaing, H. [verfasserIn] Lanet, V. [verfasserIn] Ghirardi, S. [verfasserIn] van Belkum, A. [verfasserIn] Mallard, F. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: European journal of clinical microbiology & infectious diseases - Berlin : Springer, 1982, 34(2015), 9 vom: 09. Juni, Seite 1787-1795 |
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| Übergeordnetes Werk: |
volume:34 ; year:2015 ; number:9 ; day:09 ; month:06 ; pages:1787-1795 |
| Links: |
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| DOI / URN: |
10.1007/s10096-015-2413-y |
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| Katalog-ID: |
SPR008697574 |
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| 520 | |a Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > $ 10^{5} $ CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs. | ||
| 650 | 4 | |a Urine Sample |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Bacteriuria |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Sample Preparation Method |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Differential Centrifugation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Short Growth |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Mailler, S. |e verfasserin |4 aut | |
| 700 | 1 | |a Girard, V. |e verfasserin |4 aut | |
| 700 | 1 | |a Muller, B. H. |e verfasserin |4 aut | |
| 700 | 1 | |a L’Hostis, G. |e verfasserin |4 aut | |
| 700 | 1 | |a Ducruix, C. |e verfasserin |4 aut | |
| 700 | 1 | |a Lesenne, A. |e verfasserin |4 aut | |
| 700 | 1 | |a Richez, A. |e verfasserin |4 aut | |
| 700 | 1 | |a Rostaing, H. |e verfasserin |4 aut | |
| 700 | 1 | |a Lanet, V. |e verfasserin |4 aut | |
| 700 | 1 | |a Ghirardi, S. |e verfasserin |4 aut | |
| 700 | 1 | |a van Belkum, A. |e verfasserin |4 aut | |
| 700 | 1 | |a Mallard, F. |e verfasserin |4 aut | |
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10.1007/s10096-015-2413-y doi (DE-627)SPR008697574 (SPR)s10096-015-2413-y-e DE-627 ger DE-627 rakwb eng 610 ASE 44.43 bkl 44.75 bkl Veron, L. verfasserin aut Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > $ 10^{5} $ CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs. Urine Sample (dpeaa)DE-He213 Bacteriuria (dpeaa)DE-He213 Sample Preparation Method (dpeaa)DE-He213 Differential Centrifugation (dpeaa)DE-He213 Short Growth (dpeaa)DE-He213 Mailler, S. verfasserin aut Girard, V. verfasserin aut Muller, B. H. verfasserin aut L’Hostis, G. verfasserin aut Ducruix, C. verfasserin aut Lesenne, A. verfasserin aut Richez, A. verfasserin aut Rostaing, H. verfasserin aut Lanet, V. verfasserin aut Ghirardi, S. verfasserin aut van Belkum, A. verfasserin aut Mallard, F. verfasserin aut Enthalten in European journal of clinical microbiology & infectious diseases Berlin : Springer, 1982 34(2015), 9 vom: 09. Juni, Seite 1787-1795 (DE-627)25372273X (DE-600)1459049-9 1435-4373 nnns volume:34 year:2015 number:9 day:09 month:06 pages:1787-1795 https://dx.doi.org/10.1007/s10096-015-2413-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.43 ASE 44.75 ASE AR 34 2015 9 09 06 1787-1795 |
| spelling |
10.1007/s10096-015-2413-y doi (DE-627)SPR008697574 (SPR)s10096-015-2413-y-e DE-627 ger DE-627 rakwb eng 610 ASE 44.43 bkl 44.75 bkl Veron, L. verfasserin aut Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > $ 10^{5} $ CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs. Urine Sample (dpeaa)DE-He213 Bacteriuria (dpeaa)DE-He213 Sample Preparation Method (dpeaa)DE-He213 Differential Centrifugation (dpeaa)DE-He213 Short Growth (dpeaa)DE-He213 Mailler, S. verfasserin aut Girard, V. verfasserin aut Muller, B. H. verfasserin aut L’Hostis, G. verfasserin aut Ducruix, C. verfasserin aut Lesenne, A. verfasserin aut Richez, A. verfasserin aut Rostaing, H. verfasserin aut Lanet, V. verfasserin aut Ghirardi, S. verfasserin aut van Belkum, A. verfasserin aut Mallard, F. verfasserin aut Enthalten in European journal of clinical microbiology & infectious diseases Berlin : Springer, 1982 34(2015), 9 vom: 09. Juni, Seite 1787-1795 (DE-627)25372273X (DE-600)1459049-9 1435-4373 nnns volume:34 year:2015 number:9 day:09 month:06 pages:1787-1795 https://dx.doi.org/10.1007/s10096-015-2413-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.43 ASE 44.75 ASE AR 34 2015 9 09 06 1787-1795 |
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10.1007/s10096-015-2413-y doi (DE-627)SPR008697574 (SPR)s10096-015-2413-y-e DE-627 ger DE-627 rakwb eng 610 ASE 44.43 bkl 44.75 bkl Veron, L. verfasserin aut Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > $ 10^{5} $ CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs. Urine Sample (dpeaa)DE-He213 Bacteriuria (dpeaa)DE-He213 Sample Preparation Method (dpeaa)DE-He213 Differential Centrifugation (dpeaa)DE-He213 Short Growth (dpeaa)DE-He213 Mailler, S. verfasserin aut Girard, V. verfasserin aut Muller, B. H. verfasserin aut L’Hostis, G. verfasserin aut Ducruix, C. verfasserin aut Lesenne, A. verfasserin aut Richez, A. verfasserin aut Rostaing, H. verfasserin aut Lanet, V. verfasserin aut Ghirardi, S. verfasserin aut van Belkum, A. verfasserin aut Mallard, F. verfasserin aut Enthalten in European journal of clinical microbiology & infectious diseases Berlin : Springer, 1982 34(2015), 9 vom: 09. Juni, Seite 1787-1795 (DE-627)25372273X (DE-600)1459049-9 1435-4373 nnns volume:34 year:2015 number:9 day:09 month:06 pages:1787-1795 https://dx.doi.org/10.1007/s10096-015-2413-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.43 ASE 44.75 ASE AR 34 2015 9 09 06 1787-1795 |
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10.1007/s10096-015-2413-y doi (DE-627)SPR008697574 (SPR)s10096-015-2413-y-e DE-627 ger DE-627 rakwb eng 610 ASE 44.43 bkl 44.75 bkl Veron, L. verfasserin aut Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > $ 10^{5} $ CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs. Urine Sample (dpeaa)DE-He213 Bacteriuria (dpeaa)DE-He213 Sample Preparation Method (dpeaa)DE-He213 Differential Centrifugation (dpeaa)DE-He213 Short Growth (dpeaa)DE-He213 Mailler, S. verfasserin aut Girard, V. verfasserin aut Muller, B. H. verfasserin aut L’Hostis, G. verfasserin aut Ducruix, C. verfasserin aut Lesenne, A. verfasserin aut Richez, A. verfasserin aut Rostaing, H. verfasserin aut Lanet, V. verfasserin aut Ghirardi, S. verfasserin aut van Belkum, A. verfasserin aut Mallard, F. verfasserin aut Enthalten in European journal of clinical microbiology & infectious diseases Berlin : Springer, 1982 34(2015), 9 vom: 09. Juni, Seite 1787-1795 (DE-627)25372273X (DE-600)1459049-9 1435-4373 nnns volume:34 year:2015 number:9 day:09 month:06 pages:1787-1795 https://dx.doi.org/10.1007/s10096-015-2413-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.43 ASE 44.75 ASE AR 34 2015 9 09 06 1787-1795 |
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10.1007/s10096-015-2413-y doi (DE-627)SPR008697574 (SPR)s10096-015-2413-y-e DE-627 ger DE-627 rakwb eng 610 ASE 44.43 bkl 44.75 bkl Veron, L. verfasserin aut Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > $ 10^{5} $ CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs. Urine Sample (dpeaa)DE-He213 Bacteriuria (dpeaa)DE-He213 Sample Preparation Method (dpeaa)DE-He213 Differential Centrifugation (dpeaa)DE-He213 Short Growth (dpeaa)DE-He213 Mailler, S. verfasserin aut Girard, V. verfasserin aut Muller, B. H. verfasserin aut L’Hostis, G. verfasserin aut Ducruix, C. verfasserin aut Lesenne, A. verfasserin aut Richez, A. verfasserin aut Rostaing, H. verfasserin aut Lanet, V. verfasserin aut Ghirardi, S. verfasserin aut van Belkum, A. verfasserin aut Mallard, F. verfasserin aut Enthalten in European journal of clinical microbiology & infectious diseases Berlin : Springer, 1982 34(2015), 9 vom: 09. Juni, Seite 1787-1795 (DE-627)25372273X (DE-600)1459049-9 1435-4373 nnns volume:34 year:2015 number:9 day:09 month:06 pages:1787-1795 https://dx.doi.org/10.1007/s10096-015-2413-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.43 ASE 44.75 ASE AR 34 2015 9 09 06 1787-1795 |
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Enthalten in European journal of clinical microbiology & infectious diseases 34(2015), 9 vom: 09. Juni, Seite 1787-1795 volume:34 year:2015 number:9 day:09 month:06 pages:1787-1795 |
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Veron, L. @@aut@@ Mailler, S. @@aut@@ Girard, V. @@aut@@ Muller, B. H. @@aut@@ L’Hostis, G. @@aut@@ Ducruix, C. @@aut@@ Lesenne, A. @@aut@@ Richez, A. @@aut@@ Rostaing, H. @@aut@@ Lanet, V. @@aut@@ Ghirardi, S. @@aut@@ van Belkum, A. @@aut@@ Mallard, F. @@aut@@ |
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| author |
Veron, L. |
| spellingShingle |
Veron, L. ddc 610 bkl 44.43 bkl 44.75 misc Urine Sample misc Bacteriuria misc Sample Preparation Method misc Differential Centrifugation misc Short Growth Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS |
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Veron, L. |
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electronic Article |
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610 - Medicine & health |
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keep |
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springer |
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Not Illustrated |
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1435-4373 |
| topic_title |
610 ASE 44.43 bkl 44.75 bkl Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS Urine Sample (dpeaa)DE-He213 Bacteriuria (dpeaa)DE-He213 Sample Preparation Method (dpeaa)DE-He213 Differential Centrifugation (dpeaa)DE-He213 Short Growth (dpeaa)DE-He213 |
| topic |
ddc 610 bkl 44.43 bkl 44.75 misc Urine Sample misc Bacteriuria misc Sample Preparation Method misc Differential Centrifugation misc Short Growth |
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ddc 610 bkl 44.43 bkl 44.75 misc Urine Sample misc Bacteriuria misc Sample Preparation Method misc Differential Centrifugation misc Short Growth |
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ddc 610 bkl 44.43 bkl 44.75 misc Urine Sample misc Bacteriuria misc Sample Preparation Method misc Differential Centrifugation misc Short Growth |
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Elektronische Aufsätze Aufsätze Elektronische Ressource |
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European journal of clinical microbiology & infectious diseases |
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25372273X |
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610 - Medicine & health |
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European journal of clinical microbiology & infectious diseases |
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Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS |
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Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS |
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Veron, L. |
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European journal of clinical microbiology & infectious diseases |
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European journal of clinical microbiology & infectious diseases |
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Veron, L. Mailler, S. Girard, V. Muller, B. H. L’Hostis, G. Ducruix, C. Lesenne, A. Richez, A. Rostaing, H. Lanet, V. Ghirardi, S. van Belkum, A. Mallard, F. |
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rapid urine preparation prior to identification of uropathogens by maldi-tof ms |
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Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS |
| abstract |
Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > $ 10^{5} $ CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs. |
| abstractGer |
Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > $ 10^{5} $ CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs. |
| abstract_unstemmed |
Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > $ 10^{5} $ CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs. |
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Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS |
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Mailler, S. Girard, V. Muller, B. H. L’Hostis, G. Ducruix, C. Lesenne, A. Richez, A. Rostaing, H. Lanet, V. Ghirardi, S. van Belkum, A. Mallard, F. |
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| score |
7.399008 |