Generation and functional analysis of monoclonal antibodies against the second extracellular loop of human M3 muscarinic acetylcholine receptor
Abstract The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of the...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Tsuboi, Hiroto [verfasserIn] Nakamura, Yumi [verfasserIn] Iizuka, Mana [verfasserIn] Matsuo, Naomi [verfasserIn] Matsumoto, Isao [verfasserIn] Sumida, Takayuki [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2011 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Modern rheumatology - Oxford : Oxford University Press, 2000, 22(2011), 2 vom: 30. Aug., Seite 264-271 |
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| Übergeordnetes Werk: |
volume:22 ; year:2011 ; number:2 ; day:30 ; month:08 ; pages:264-271 |
| Links: |
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| DOI / URN: |
10.1007/s10165-011-0514-8 |
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| Katalog-ID: |
SPR009025626 |
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| 520 | |a Abstract The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren’s syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-$ M3R^{2nd} $ mAbs) and analyzed their function by $ Ca^{2+} $-influx assays, using a human salivary gland (HSG) cell line. These two anti-$ M3R^{2nd} $ mAbs suppressed $ Ca^{2+} $-influx in the HSG cells induced by cevimeline stimulation, suggesting that autoantibodies against the second extracellular loop of M3R could be involved in salivary dysfunction in patients with SS. | ||
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| 650 | 4 | |a M3 muscarinic acetylcholine receptor |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Salivary secretion |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Sjögren’s syndrome |7 (dpeaa)DE-He213 | |
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10.1007/s10165-011-0514-8 doi (DE-627)SPR009025626 (SPR)s10165-011-0514-8-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE 44.83 bkl Tsuboi, Hiroto verfasserin aut Generation and functional analysis of monoclonal antibodies against the second extracellular loop of human M3 muscarinic acetylcholine receptor 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren’s syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-$ M3R^{2nd} $ mAbs) and analyzed their function by $ Ca^{2+} $-influx assays, using a human salivary gland (HSG) cell line. These two anti-$ M3R^{2nd} $ mAbs suppressed $ Ca^{2+} $-influx in the HSG cells induced by cevimeline stimulation, suggesting that autoantibodies against the second extracellular loop of M3R could be involved in salivary dysfunction in patients with SS. Autoantibodies (dpeaa)DE-He213 Ca (dpeaa)DE-He213 -influx (dpeaa)DE-He213 M3 muscarinic acetylcholine receptor (dpeaa)DE-He213 Salivary secretion (dpeaa)DE-He213 Sjögren’s syndrome (dpeaa)DE-He213 Nakamura, Yumi verfasserin aut Iizuka, Mana verfasserin aut Matsuo, Naomi verfasserin aut Matsumoto, Isao verfasserin aut Sumida, Takayuki verfasserin aut Enthalten in Modern rheumatology Oxford : Oxford University Press, 2000 22(2011), 2 vom: 30. Aug., Seite 264-271 (DE-627)320627497 (DE-600)2023498-3 1439-7609 nnns volume:22 year:2011 number:2 day:30 month:08 pages:264-271 https://dx.doi.org/10.1007/s10165-011-0514-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_151 GBV_ILN_170 GBV_ILN_224 GBV_ILN_267 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2026 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4328 GBV_ILN_4333 44.83 ASE AR 22 2011 2 30 08 264-271 |
| spelling |
10.1007/s10165-011-0514-8 doi (DE-627)SPR009025626 (SPR)s10165-011-0514-8-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE 44.83 bkl Tsuboi, Hiroto verfasserin aut Generation and functional analysis of monoclonal antibodies against the second extracellular loop of human M3 muscarinic acetylcholine receptor 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren’s syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-$ M3R^{2nd} $ mAbs) and analyzed their function by $ Ca^{2+} $-influx assays, using a human salivary gland (HSG) cell line. These two anti-$ M3R^{2nd} $ mAbs suppressed $ Ca^{2+} $-influx in the HSG cells induced by cevimeline stimulation, suggesting that autoantibodies against the second extracellular loop of M3R could be involved in salivary dysfunction in patients with SS. Autoantibodies (dpeaa)DE-He213 Ca (dpeaa)DE-He213 -influx (dpeaa)DE-He213 M3 muscarinic acetylcholine receptor (dpeaa)DE-He213 Salivary secretion (dpeaa)DE-He213 Sjögren’s syndrome (dpeaa)DE-He213 Nakamura, Yumi verfasserin aut Iizuka, Mana verfasserin aut Matsuo, Naomi verfasserin aut Matsumoto, Isao verfasserin aut Sumida, Takayuki verfasserin aut Enthalten in Modern rheumatology Oxford : Oxford University Press, 2000 22(2011), 2 vom: 30. Aug., Seite 264-271 (DE-627)320627497 (DE-600)2023498-3 1439-7609 nnns volume:22 year:2011 number:2 day:30 month:08 pages:264-271 https://dx.doi.org/10.1007/s10165-011-0514-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_151 GBV_ILN_170 GBV_ILN_224 GBV_ILN_267 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2026 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4328 GBV_ILN_4333 44.83 ASE AR 22 2011 2 30 08 264-271 |
| allfields_unstemmed |
10.1007/s10165-011-0514-8 doi (DE-627)SPR009025626 (SPR)s10165-011-0514-8-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE 44.83 bkl Tsuboi, Hiroto verfasserin aut Generation and functional analysis of monoclonal antibodies against the second extracellular loop of human M3 muscarinic acetylcholine receptor 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren’s syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-$ M3R^{2nd} $ mAbs) and analyzed their function by $ Ca^{2+} $-influx assays, using a human salivary gland (HSG) cell line. These two anti-$ M3R^{2nd} $ mAbs suppressed $ Ca^{2+} $-influx in the HSG cells induced by cevimeline stimulation, suggesting that autoantibodies against the second extracellular loop of M3R could be involved in salivary dysfunction in patients with SS. Autoantibodies (dpeaa)DE-He213 Ca (dpeaa)DE-He213 -influx (dpeaa)DE-He213 M3 muscarinic acetylcholine receptor (dpeaa)DE-He213 Salivary secretion (dpeaa)DE-He213 Sjögren’s syndrome (dpeaa)DE-He213 Nakamura, Yumi verfasserin aut Iizuka, Mana verfasserin aut Matsuo, Naomi verfasserin aut Matsumoto, Isao verfasserin aut Sumida, Takayuki verfasserin aut Enthalten in Modern rheumatology Oxford : Oxford University Press, 2000 22(2011), 2 vom: 30. Aug., Seite 264-271 (DE-627)320627497 (DE-600)2023498-3 1439-7609 nnns volume:22 year:2011 number:2 day:30 month:08 pages:264-271 https://dx.doi.org/10.1007/s10165-011-0514-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_151 GBV_ILN_170 GBV_ILN_224 GBV_ILN_267 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2026 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4328 GBV_ILN_4333 44.83 ASE AR 22 2011 2 30 08 264-271 |
| allfieldsGer |
10.1007/s10165-011-0514-8 doi (DE-627)SPR009025626 (SPR)s10165-011-0514-8-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE 44.83 bkl Tsuboi, Hiroto verfasserin aut Generation and functional analysis of monoclonal antibodies against the second extracellular loop of human M3 muscarinic acetylcholine receptor 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren’s syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-$ M3R^{2nd} $ mAbs) and analyzed their function by $ Ca^{2+} $-influx assays, using a human salivary gland (HSG) cell line. These two anti-$ M3R^{2nd} $ mAbs suppressed $ Ca^{2+} $-influx in the HSG cells induced by cevimeline stimulation, suggesting that autoantibodies against the second extracellular loop of M3R could be involved in salivary dysfunction in patients with SS. Autoantibodies (dpeaa)DE-He213 Ca (dpeaa)DE-He213 -influx (dpeaa)DE-He213 M3 muscarinic acetylcholine receptor (dpeaa)DE-He213 Salivary secretion (dpeaa)DE-He213 Sjögren’s syndrome (dpeaa)DE-He213 Nakamura, Yumi verfasserin aut Iizuka, Mana verfasserin aut Matsuo, Naomi verfasserin aut Matsumoto, Isao verfasserin aut Sumida, Takayuki verfasserin aut Enthalten in Modern rheumatology Oxford : Oxford University Press, 2000 22(2011), 2 vom: 30. Aug., Seite 264-271 (DE-627)320627497 (DE-600)2023498-3 1439-7609 nnns volume:22 year:2011 number:2 day:30 month:08 pages:264-271 https://dx.doi.org/10.1007/s10165-011-0514-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_151 GBV_ILN_170 GBV_ILN_224 GBV_ILN_267 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2026 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4328 GBV_ILN_4333 44.83 ASE AR 22 2011 2 30 08 264-271 |
| allfieldsSound |
10.1007/s10165-011-0514-8 doi (DE-627)SPR009025626 (SPR)s10165-011-0514-8-e DE-627 ger DE-627 rakwb eng 610 ASE 610 ASE 44.83 bkl Tsuboi, Hiroto verfasserin aut Generation and functional analysis of monoclonal antibodies against the second extracellular loop of human M3 muscarinic acetylcholine receptor 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren’s syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-$ M3R^{2nd} $ mAbs) and analyzed their function by $ Ca^{2+} $-influx assays, using a human salivary gland (HSG) cell line. These two anti-$ M3R^{2nd} $ mAbs suppressed $ Ca^{2+} $-influx in the HSG cells induced by cevimeline stimulation, suggesting that autoantibodies against the second extracellular loop of M3R could be involved in salivary dysfunction in patients with SS. Autoantibodies (dpeaa)DE-He213 Ca (dpeaa)DE-He213 -influx (dpeaa)DE-He213 M3 muscarinic acetylcholine receptor (dpeaa)DE-He213 Salivary secretion (dpeaa)DE-He213 Sjögren’s syndrome (dpeaa)DE-He213 Nakamura, Yumi verfasserin aut Iizuka, Mana verfasserin aut Matsuo, Naomi verfasserin aut Matsumoto, Isao verfasserin aut Sumida, Takayuki verfasserin aut Enthalten in Modern rheumatology Oxford : Oxford University Press, 2000 22(2011), 2 vom: 30. Aug., Seite 264-271 (DE-627)320627497 (DE-600)2023498-3 1439-7609 nnns volume:22 year:2011 number:2 day:30 month:08 pages:264-271 https://dx.doi.org/10.1007/s10165-011-0514-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_151 GBV_ILN_170 GBV_ILN_224 GBV_ILN_267 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2026 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4328 GBV_ILN_4333 44.83 ASE AR 22 2011 2 30 08 264-271 |
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Enthalten in Modern rheumatology 22(2011), 2 vom: 30. Aug., Seite 264-271 volume:22 year:2011 number:2 day:30 month:08 pages:264-271 |
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Tsuboi, Hiroto |
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Tsuboi, Hiroto ddc 610 bkl 44.83 misc Autoantibodies misc Ca misc -influx misc M3 muscarinic acetylcholine receptor misc Salivary secretion misc Sjögren’s syndrome Generation and functional analysis of monoclonal antibodies against the second extracellular loop of human M3 muscarinic acetylcholine receptor |
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generation and functional analysis of monoclonal antibodies against the second extracellular loop of human m3 muscarinic acetylcholine receptor |
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Generation and functional analysis of monoclonal antibodies against the second extracellular loop of human M3 muscarinic acetylcholine receptor |
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Abstract The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren’s syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-$ M3R^{2nd} $ mAbs) and analyzed their function by $ Ca^{2+} $-influx assays, using a human salivary gland (HSG) cell line. These two anti-$ M3R^{2nd} $ mAbs suppressed $ Ca^{2+} $-influx in the HSG cells induced by cevimeline stimulation, suggesting that autoantibodies against the second extracellular loop of M3R could be involved in salivary dysfunction in patients with SS. |
| abstractGer |
Abstract The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren’s syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-$ M3R^{2nd} $ mAbs) and analyzed their function by $ Ca^{2+} $-influx assays, using a human salivary gland (HSG) cell line. These two anti-$ M3R^{2nd} $ mAbs suppressed $ Ca^{2+} $-influx in the HSG cells induced by cevimeline stimulation, suggesting that autoantibodies against the second extracellular loop of M3R could be involved in salivary dysfunction in patients with SS. |
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Abstract The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren’s syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-$ M3R^{2nd} $ mAbs) and analyzed their function by $ Ca^{2+} $-influx assays, using a human salivary gland (HSG) cell line. These two anti-$ M3R^{2nd} $ mAbs suppressed $ Ca^{2+} $-influx in the HSG cells induced by cevimeline stimulation, suggesting that autoantibodies against the second extracellular loop of M3R could be involved in salivary dysfunction in patients with SS. |
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The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren’s syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-$ M3R^{2nd} $ mAbs) and analyzed their function by $ Ca^{2+} $-influx assays, using a human salivary gland (HSG) cell line. 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