High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity
Abstract We describe a strategy to establish cyanobacterial strains with high levels of $ H_{2} $ production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other hetero...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yoshino, Fuminori [verfasserIn] Ikeda, Hiroshi [verfasserIn] Masukawa, Hajime [verfasserIn] Sakurai, Hidehiro [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2006 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Marine biotechnology - New York, NY : Springer, 1999, 9(2006), 1 vom: 28. Nov., Seite 101-112 |
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| Übergeordnetes Werk: |
volume:9 ; year:2006 ; number:1 ; day:28 ; month:11 ; pages:101-112 |
| Links: |
|---|
| DOI / URN: |
10.1007/s10126-006-6035-3 |
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| Katalog-ID: |
SPR009355758 |
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| 100 | 1 | |a Yoshino, Fuminori |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity |
| 264 | 1 | |c 2006 | |
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| 520 | |a Abstract We describe a strategy to establish cyanobacterial strains with high levels of $ H_{2} $ production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced $ H_{2} $ at 100 μmoles mg chlorophyll a-1 $ h^{-1} $, a rate three times that of the wild-type. The ΔhupL cells could accumulate $ H_{2} $ to about 29% (v/v) accompanied by $ O_{2} $ evolution in 6 days, under a starting gas phase of Ar + 5% $ CO_{2} $. The presence of 20% $ O_{2} $ in the initial gas phase inhibited $ H_{2} $ accumulation of the ΔhupL cells by less than 20% until day 7. | ||
| 650 | 4 | |a Cyanobacteria |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a hydrogen |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a hydrogenase |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a nitrogenase |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a photobiological H |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a production |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Ikeda, Hiroshi |e verfasserin |4 aut | |
| 700 | 1 | |a Masukawa, Hajime |e verfasserin |4 aut | |
| 700 | 1 | |a Sakurai, Hidehiro |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Marine biotechnology |d New York, NY : Springer, 1999 |g 9(2006), 1 vom: 28. Nov., Seite 101-112 |w (DE-627)271349891 |w (DE-600)1479877-3 |x 1436-2236 |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2006 |g number:1 |g day:28 |g month:11 |g pages:101-112 |
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| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
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| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
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| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4012 | ||
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| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
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| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
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| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
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| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
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| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
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| allfields |
10.1007/s10126-006-6035-3 doi (DE-627)SPR009355758 (SPR)s10126-006-6035-3-e DE-627 ger DE-627 rakwb eng 660 570 ASE 570 550 ASE 58.30 bkl 42.94 bkl Yoshino, Fuminori verfasserin aut High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We describe a strategy to establish cyanobacterial strains with high levels of $ H_{2} $ production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced $ H_{2} $ at 100 μmoles mg chlorophyll a-1 $ h^{-1} $, a rate three times that of the wild-type. The ΔhupL cells could accumulate $ H_{2} $ to about 29% (v/v) accompanied by $ O_{2} $ evolution in 6 days, under a starting gas phase of Ar + 5% $ CO_{2} $. The presence of 20% $ O_{2} $ in the initial gas phase inhibited $ H_{2} $ accumulation of the ΔhupL cells by less than 20% until day 7. Cyanobacteria (dpeaa)DE-He213 hydrogen (dpeaa)DE-He213 hydrogenase (dpeaa)DE-He213 nitrogenase (dpeaa)DE-He213 photobiological H (dpeaa)DE-He213 production (dpeaa)DE-He213 Ikeda, Hiroshi verfasserin aut Masukawa, Hajime verfasserin aut Sakurai, Hidehiro verfasserin aut Enthalten in Marine biotechnology New York, NY : Springer, 1999 9(2006), 1 vom: 28. Nov., Seite 101-112 (DE-627)271349891 (DE-600)1479877-3 1436-2236 nnns volume:9 year:2006 number:1 day:28 month:11 pages:101-112 https://dx.doi.org/10.1007/s10126-006-6035-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.30 ASE 42.94 ASE AR 9 2006 1 28 11 101-112 |
| spelling |
10.1007/s10126-006-6035-3 doi (DE-627)SPR009355758 (SPR)s10126-006-6035-3-e DE-627 ger DE-627 rakwb eng 660 570 ASE 570 550 ASE 58.30 bkl 42.94 bkl Yoshino, Fuminori verfasserin aut High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We describe a strategy to establish cyanobacterial strains with high levels of $ H_{2} $ production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced $ H_{2} $ at 100 μmoles mg chlorophyll a-1 $ h^{-1} $, a rate three times that of the wild-type. The ΔhupL cells could accumulate $ H_{2} $ to about 29% (v/v) accompanied by $ O_{2} $ evolution in 6 days, under a starting gas phase of Ar + 5% $ CO_{2} $. The presence of 20% $ O_{2} $ in the initial gas phase inhibited $ H_{2} $ accumulation of the ΔhupL cells by less than 20% until day 7. Cyanobacteria (dpeaa)DE-He213 hydrogen (dpeaa)DE-He213 hydrogenase (dpeaa)DE-He213 nitrogenase (dpeaa)DE-He213 photobiological H (dpeaa)DE-He213 production (dpeaa)DE-He213 Ikeda, Hiroshi verfasserin aut Masukawa, Hajime verfasserin aut Sakurai, Hidehiro verfasserin aut Enthalten in Marine biotechnology New York, NY : Springer, 1999 9(2006), 1 vom: 28. Nov., Seite 101-112 (DE-627)271349891 (DE-600)1479877-3 1436-2236 nnns volume:9 year:2006 number:1 day:28 month:11 pages:101-112 https://dx.doi.org/10.1007/s10126-006-6035-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.30 ASE 42.94 ASE AR 9 2006 1 28 11 101-112 |
| allfields_unstemmed |
10.1007/s10126-006-6035-3 doi (DE-627)SPR009355758 (SPR)s10126-006-6035-3-e DE-627 ger DE-627 rakwb eng 660 570 ASE 570 550 ASE 58.30 bkl 42.94 bkl Yoshino, Fuminori verfasserin aut High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We describe a strategy to establish cyanobacterial strains with high levels of $ H_{2} $ production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced $ H_{2} $ at 100 μmoles mg chlorophyll a-1 $ h^{-1} $, a rate three times that of the wild-type. The ΔhupL cells could accumulate $ H_{2} $ to about 29% (v/v) accompanied by $ O_{2} $ evolution in 6 days, under a starting gas phase of Ar + 5% $ CO_{2} $. The presence of 20% $ O_{2} $ in the initial gas phase inhibited $ H_{2} $ accumulation of the ΔhupL cells by less than 20% until day 7. Cyanobacteria (dpeaa)DE-He213 hydrogen (dpeaa)DE-He213 hydrogenase (dpeaa)DE-He213 nitrogenase (dpeaa)DE-He213 photobiological H (dpeaa)DE-He213 production (dpeaa)DE-He213 Ikeda, Hiroshi verfasserin aut Masukawa, Hajime verfasserin aut Sakurai, Hidehiro verfasserin aut Enthalten in Marine biotechnology New York, NY : Springer, 1999 9(2006), 1 vom: 28. Nov., Seite 101-112 (DE-627)271349891 (DE-600)1479877-3 1436-2236 nnns volume:9 year:2006 number:1 day:28 month:11 pages:101-112 https://dx.doi.org/10.1007/s10126-006-6035-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.30 ASE 42.94 ASE AR 9 2006 1 28 11 101-112 |
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10.1007/s10126-006-6035-3 doi (DE-627)SPR009355758 (SPR)s10126-006-6035-3-e DE-627 ger DE-627 rakwb eng 660 570 ASE 570 550 ASE 58.30 bkl 42.94 bkl Yoshino, Fuminori verfasserin aut High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We describe a strategy to establish cyanobacterial strains with high levels of $ H_{2} $ production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced $ H_{2} $ at 100 μmoles mg chlorophyll a-1 $ h^{-1} $, a rate three times that of the wild-type. The ΔhupL cells could accumulate $ H_{2} $ to about 29% (v/v) accompanied by $ O_{2} $ evolution in 6 days, under a starting gas phase of Ar + 5% $ CO_{2} $. The presence of 20% $ O_{2} $ in the initial gas phase inhibited $ H_{2} $ accumulation of the ΔhupL cells by less than 20% until day 7. Cyanobacteria (dpeaa)DE-He213 hydrogen (dpeaa)DE-He213 hydrogenase (dpeaa)DE-He213 nitrogenase (dpeaa)DE-He213 photobiological H (dpeaa)DE-He213 production (dpeaa)DE-He213 Ikeda, Hiroshi verfasserin aut Masukawa, Hajime verfasserin aut Sakurai, Hidehiro verfasserin aut Enthalten in Marine biotechnology New York, NY : Springer, 1999 9(2006), 1 vom: 28. Nov., Seite 101-112 (DE-627)271349891 (DE-600)1479877-3 1436-2236 nnns volume:9 year:2006 number:1 day:28 month:11 pages:101-112 https://dx.doi.org/10.1007/s10126-006-6035-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.30 ASE 42.94 ASE AR 9 2006 1 28 11 101-112 |
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10.1007/s10126-006-6035-3 doi (DE-627)SPR009355758 (SPR)s10126-006-6035-3-e DE-627 ger DE-627 rakwb eng 660 570 ASE 570 550 ASE 58.30 bkl 42.94 bkl Yoshino, Fuminori verfasserin aut High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We describe a strategy to establish cyanobacterial strains with high levels of $ H_{2} $ production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced $ H_{2} $ at 100 μmoles mg chlorophyll a-1 $ h^{-1} $, a rate three times that of the wild-type. The ΔhupL cells could accumulate $ H_{2} $ to about 29% (v/v) accompanied by $ O_{2} $ evolution in 6 days, under a starting gas phase of Ar + 5% $ CO_{2} $. The presence of 20% $ O_{2} $ in the initial gas phase inhibited $ H_{2} $ accumulation of the ΔhupL cells by less than 20% until day 7. Cyanobacteria (dpeaa)DE-He213 hydrogen (dpeaa)DE-He213 hydrogenase (dpeaa)DE-He213 nitrogenase (dpeaa)DE-He213 photobiological H (dpeaa)DE-He213 production (dpeaa)DE-He213 Ikeda, Hiroshi verfasserin aut Masukawa, Hajime verfasserin aut Sakurai, Hidehiro verfasserin aut Enthalten in Marine biotechnology New York, NY : Springer, 1999 9(2006), 1 vom: 28. Nov., Seite 101-112 (DE-627)271349891 (DE-600)1479877-3 1436-2236 nnns volume:9 year:2006 number:1 day:28 month:11 pages:101-112 https://dx.doi.org/10.1007/s10126-006-6035-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.30 ASE 42.94 ASE AR 9 2006 1 28 11 101-112 |
| language |
English |
| source |
Enthalten in Marine biotechnology 9(2006), 1 vom: 28. Nov., Seite 101-112 volume:9 year:2006 number:1 day:28 month:11 pages:101-112 |
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Enthalten in Marine biotechnology 9(2006), 1 vom: 28. Nov., Seite 101-112 volume:9 year:2006 number:1 day:28 month:11 pages:101-112 |
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findex.gbv.de |
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Cyanobacteria hydrogen hydrogenase nitrogenase photobiological H production |
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Marine biotechnology |
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Yoshino, Fuminori @@aut@@ Ikeda, Hiroshi @@aut@@ Masukawa, Hajime @@aut@@ Sakurai, Hidehiro @@aut@@ |
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2006-11-28T00:00:00Z |
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PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced $ H_{2} $ at 100 μmoles mg chlorophyll a-1 $ h^{-1} $, a rate three times that of the wild-type. The ΔhupL cells could accumulate $ H_{2} $ to about 29% (v/v) accompanied by $ O_{2} $ evolution in 6 days, under a starting gas phase of Ar + 5% $ CO_{2} $. 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Yoshino, Fuminori |
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Yoshino, Fuminori ddc 660 ddc 570 bkl 58.30 bkl 42.94 misc Cyanobacteria misc hydrogen misc hydrogenase misc nitrogenase misc photobiological H misc production High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity |
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660 570 ASE 570 550 ASE 58.30 bkl 42.94 bkl High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity Cyanobacteria (dpeaa)DE-He213 hydrogen (dpeaa)DE-He213 hydrogenase (dpeaa)DE-He213 nitrogenase (dpeaa)DE-He213 photobiological H (dpeaa)DE-He213 production (dpeaa)DE-He213 |
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ddc 660 ddc 570 bkl 58.30 bkl 42.94 misc Cyanobacteria misc hydrogen misc hydrogenase misc nitrogenase misc photobiological H misc production |
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High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity |
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Yoshino, Fuminori Ikeda, Hiroshi Masukawa, Hajime Sakurai, Hidehiro |
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Yoshino, Fuminori |
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high photobiological hydrogen production activity of a nostoc sp. pcc 7422 uptake hydrogenase-deficient mutant with high nitrogenase activity |
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High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity |
| abstract |
Abstract We describe a strategy to establish cyanobacterial strains with high levels of $ H_{2} $ production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced $ H_{2} $ at 100 μmoles mg chlorophyll a-1 $ h^{-1} $, a rate three times that of the wild-type. The ΔhupL cells could accumulate $ H_{2} $ to about 29% (v/v) accompanied by $ O_{2} $ evolution in 6 days, under a starting gas phase of Ar + 5% $ CO_{2} $. The presence of 20% $ O_{2} $ in the initial gas phase inhibited $ H_{2} $ accumulation of the ΔhupL cells by less than 20% until day 7. |
| abstractGer |
Abstract We describe a strategy to establish cyanobacterial strains with high levels of $ H_{2} $ production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced $ H_{2} $ at 100 μmoles mg chlorophyll a-1 $ h^{-1} $, a rate three times that of the wild-type. The ΔhupL cells could accumulate $ H_{2} $ to about 29% (v/v) accompanied by $ O_{2} $ evolution in 6 days, under a starting gas phase of Ar + 5% $ CO_{2} $. The presence of 20% $ O_{2} $ in the initial gas phase inhibited $ H_{2} $ accumulation of the ΔhupL cells by less than 20% until day 7. |
| abstract_unstemmed |
Abstract We describe a strategy to establish cyanobacterial strains with high levels of $ H_{2} $ production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. Nostoc sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (ΔhupL) by insertional disruption of the hupL gene. The ΔhupL mutant produced $ H_{2} $ at 100 μmoles mg chlorophyll a-1 $ h^{-1} $, a rate three times that of the wild-type. The ΔhupL cells could accumulate $ H_{2} $ to about 29% (v/v) accompanied by $ O_{2} $ evolution in 6 days, under a starting gas phase of Ar + 5% $ CO_{2} $. The presence of 20% $ O_{2} $ in the initial gas phase inhibited $ H_{2} $ accumulation of the ΔhupL cells by less than 20% until day 7. |
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High Photobiological Hydrogen Production Activity of a Nostoc sp. PCC 7422 Uptake Hydrogenase-Deficient Mutant with High Nitrogenase Activity |
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| score |
7.399867 |