Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW
Abstract Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in l-isoleucine uptake rates, while overexpression of brnFE leads to enhanced l-isoleucine export rates. Given that net excretion rates would be an importa...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Xie, Xixian [verfasserIn] Xu, Lanlan [verfasserIn] Shi, Jianming [verfasserIn] Xu, Qingyang [verfasserIn] Chen, Ning [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2012 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of industrial microbiology and biotechnology - Berlin : Springer, 1986, 39(2012), 10 vom: 26. Juni, Seite 1549-1556 |
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| Übergeordnetes Werk: |
volume:39 ; year:2012 ; number:10 ; day:26 ; month:06 ; pages:1549-1556 |
| Links: |
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| DOI / URN: |
10.1007/s10295-012-1155-4 |
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| Katalog-ID: |
SPR009373039 |
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| 100 | 1 | |a Xie, Xixian |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW |
| 264 | 1 | |c 2012 | |
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| 520 | |a Abstract Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in l-isoleucine uptake rates, while overexpression of brnFE leads to enhanced l-isoleucine export rates. Given that net excretion rates would be an important factor for high titers of l-isoleucine accumulation, we have tested the notion that decreased l-isoleucine uptake combined with increased l-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of l-isoleucine. To examine the effect of the two carriers on l-isoleucine accumulation in l-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol $ min^{−1} $ mg (dry wt)−1 and its l-isoleucine titer increased from 154.3 mM (20.2 g $ l^{−1} $) to 170.3 mM (22.3 g $ l^{−1} $). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol $ min^{−1} $ mg (dry wt)−1, and their l-isoleucine production titers were 197.2 mM (25.9 g $ l^{−1} $) and 221.0 mM (29.0 g $ l^{−1} $), respectively. Our results suggest that modifications of the transport system could provide a promising avenue for further increasing l-isoleucine yield in the l-isoleucine producer. | ||
| 650 | 4 | |a -isoleucine |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Excretion |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Uptake carrier |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Export carrier |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Xu, Lanlan |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Jianming |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Qingyang |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Ning |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of industrial microbiology and biotechnology |d Berlin : Springer, 1986 |g 39(2012), 10 vom: 26. Juni, Seite 1549-1556 |w (DE-627)300589514 |w (DE-600)1482484-X |x 1476-5535 |7 nnns |
| 773 | 1 | 8 | |g volume:39 |g year:2012 |g number:10 |g day:26 |g month:06 |g pages:1549-1556 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s10295-012-1155-4 |z lizenzpflichtig |3 Volltext |
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10.1007/s10295-012-1155-4 doi (DE-627)SPR009373039 (SPR)s10295-012-1155-4-e DE-627 ger DE-627 rakwb eng 570 ASE 42.30 bkl 58.00 bkl Xie, Xixian verfasserin aut Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in l-isoleucine uptake rates, while overexpression of brnFE leads to enhanced l-isoleucine export rates. Given that net excretion rates would be an important factor for high titers of l-isoleucine accumulation, we have tested the notion that decreased l-isoleucine uptake combined with increased l-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of l-isoleucine. To examine the effect of the two carriers on l-isoleucine accumulation in l-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol $ min^{−1} $ mg (dry wt)−1 and its l-isoleucine titer increased from 154.3 mM (20.2 g $ l^{−1} $) to 170.3 mM (22.3 g $ l^{−1} $). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol $ min^{−1} $ mg (dry wt)−1, and their l-isoleucine production titers were 197.2 mM (25.9 g $ l^{−1} $) and 221.0 mM (29.0 g $ l^{−1} $), respectively. Our results suggest that modifications of the transport system could provide a promising avenue for further increasing l-isoleucine yield in the l-isoleucine producer. -isoleucine (dpeaa)DE-He213 Excretion (dpeaa)DE-He213 Uptake carrier (dpeaa)DE-He213 Export carrier (dpeaa)DE-He213 Xu, Lanlan verfasserin aut Shi, Jianming verfasserin aut Xu, Qingyang verfasserin aut Chen, Ning verfasserin aut Enthalten in Journal of industrial microbiology and biotechnology Berlin : Springer, 1986 39(2012), 10 vom: 26. Juni, Seite 1549-1556 (DE-627)300589514 (DE-600)1482484-X 1476-5535 nnns volume:39 year:2012 number:10 day:26 month:06 pages:1549-1556 https://dx.doi.org/10.1007/s10295-012-1155-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.00 ASE AR 39 2012 10 26 06 1549-1556 |
| spelling |
10.1007/s10295-012-1155-4 doi (DE-627)SPR009373039 (SPR)s10295-012-1155-4-e DE-627 ger DE-627 rakwb eng 570 ASE 42.30 bkl 58.00 bkl Xie, Xixian verfasserin aut Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in l-isoleucine uptake rates, while overexpression of brnFE leads to enhanced l-isoleucine export rates. Given that net excretion rates would be an important factor for high titers of l-isoleucine accumulation, we have tested the notion that decreased l-isoleucine uptake combined with increased l-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of l-isoleucine. To examine the effect of the two carriers on l-isoleucine accumulation in l-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol $ min^{−1} $ mg (dry wt)−1 and its l-isoleucine titer increased from 154.3 mM (20.2 g $ l^{−1} $) to 170.3 mM (22.3 g $ l^{−1} $). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol $ min^{−1} $ mg (dry wt)−1, and their l-isoleucine production titers were 197.2 mM (25.9 g $ l^{−1} $) and 221.0 mM (29.0 g $ l^{−1} $), respectively. Our results suggest that modifications of the transport system could provide a promising avenue for further increasing l-isoleucine yield in the l-isoleucine producer. -isoleucine (dpeaa)DE-He213 Excretion (dpeaa)DE-He213 Uptake carrier (dpeaa)DE-He213 Export carrier (dpeaa)DE-He213 Xu, Lanlan verfasserin aut Shi, Jianming verfasserin aut Xu, Qingyang verfasserin aut Chen, Ning verfasserin aut Enthalten in Journal of industrial microbiology and biotechnology Berlin : Springer, 1986 39(2012), 10 vom: 26. Juni, Seite 1549-1556 (DE-627)300589514 (DE-600)1482484-X 1476-5535 nnns volume:39 year:2012 number:10 day:26 month:06 pages:1549-1556 https://dx.doi.org/10.1007/s10295-012-1155-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.00 ASE AR 39 2012 10 26 06 1549-1556 |
| allfields_unstemmed |
10.1007/s10295-012-1155-4 doi (DE-627)SPR009373039 (SPR)s10295-012-1155-4-e DE-627 ger DE-627 rakwb eng 570 ASE 42.30 bkl 58.00 bkl Xie, Xixian verfasserin aut Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in l-isoleucine uptake rates, while overexpression of brnFE leads to enhanced l-isoleucine export rates. Given that net excretion rates would be an important factor for high titers of l-isoleucine accumulation, we have tested the notion that decreased l-isoleucine uptake combined with increased l-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of l-isoleucine. To examine the effect of the two carriers on l-isoleucine accumulation in l-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol $ min^{−1} $ mg (dry wt)−1 and its l-isoleucine titer increased from 154.3 mM (20.2 g $ l^{−1} $) to 170.3 mM (22.3 g $ l^{−1} $). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol $ min^{−1} $ mg (dry wt)−1, and their l-isoleucine production titers were 197.2 mM (25.9 g $ l^{−1} $) and 221.0 mM (29.0 g $ l^{−1} $), respectively. Our results suggest that modifications of the transport system could provide a promising avenue for further increasing l-isoleucine yield in the l-isoleucine producer. -isoleucine (dpeaa)DE-He213 Excretion (dpeaa)DE-He213 Uptake carrier (dpeaa)DE-He213 Export carrier (dpeaa)DE-He213 Xu, Lanlan verfasserin aut Shi, Jianming verfasserin aut Xu, Qingyang verfasserin aut Chen, Ning verfasserin aut Enthalten in Journal of industrial microbiology and biotechnology Berlin : Springer, 1986 39(2012), 10 vom: 26. Juni, Seite 1549-1556 (DE-627)300589514 (DE-600)1482484-X 1476-5535 nnns volume:39 year:2012 number:10 day:26 month:06 pages:1549-1556 https://dx.doi.org/10.1007/s10295-012-1155-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.00 ASE AR 39 2012 10 26 06 1549-1556 |
| allfieldsGer |
10.1007/s10295-012-1155-4 doi (DE-627)SPR009373039 (SPR)s10295-012-1155-4-e DE-627 ger DE-627 rakwb eng 570 ASE 42.30 bkl 58.00 bkl Xie, Xixian verfasserin aut Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in l-isoleucine uptake rates, while overexpression of brnFE leads to enhanced l-isoleucine export rates. Given that net excretion rates would be an important factor for high titers of l-isoleucine accumulation, we have tested the notion that decreased l-isoleucine uptake combined with increased l-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of l-isoleucine. To examine the effect of the two carriers on l-isoleucine accumulation in l-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol $ min^{−1} $ mg (dry wt)−1 and its l-isoleucine titer increased from 154.3 mM (20.2 g $ l^{−1} $) to 170.3 mM (22.3 g $ l^{−1} $). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol $ min^{−1} $ mg (dry wt)−1, and their l-isoleucine production titers were 197.2 mM (25.9 g $ l^{−1} $) and 221.0 mM (29.0 g $ l^{−1} $), respectively. Our results suggest that modifications of the transport system could provide a promising avenue for further increasing l-isoleucine yield in the l-isoleucine producer. -isoleucine (dpeaa)DE-He213 Excretion (dpeaa)DE-He213 Uptake carrier (dpeaa)DE-He213 Export carrier (dpeaa)DE-He213 Xu, Lanlan verfasserin aut Shi, Jianming verfasserin aut Xu, Qingyang verfasserin aut Chen, Ning verfasserin aut Enthalten in Journal of industrial microbiology and biotechnology Berlin : Springer, 1986 39(2012), 10 vom: 26. Juni, Seite 1549-1556 (DE-627)300589514 (DE-600)1482484-X 1476-5535 nnns volume:39 year:2012 number:10 day:26 month:06 pages:1549-1556 https://dx.doi.org/10.1007/s10295-012-1155-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.00 ASE AR 39 2012 10 26 06 1549-1556 |
| allfieldsSound |
10.1007/s10295-012-1155-4 doi (DE-627)SPR009373039 (SPR)s10295-012-1155-4-e DE-627 ger DE-627 rakwb eng 570 ASE 42.30 bkl 58.00 bkl Xie, Xixian verfasserin aut Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in l-isoleucine uptake rates, while overexpression of brnFE leads to enhanced l-isoleucine export rates. Given that net excretion rates would be an important factor for high titers of l-isoleucine accumulation, we have tested the notion that decreased l-isoleucine uptake combined with increased l-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of l-isoleucine. To examine the effect of the two carriers on l-isoleucine accumulation in l-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol $ min^{−1} $ mg (dry wt)−1 and its l-isoleucine titer increased from 154.3 mM (20.2 g $ l^{−1} $) to 170.3 mM (22.3 g $ l^{−1} $). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol $ min^{−1} $ mg (dry wt)−1, and their l-isoleucine production titers were 197.2 mM (25.9 g $ l^{−1} $) and 221.0 mM (29.0 g $ l^{−1} $), respectively. Our results suggest that modifications of the transport system could provide a promising avenue for further increasing l-isoleucine yield in the l-isoleucine producer. -isoleucine (dpeaa)DE-He213 Excretion (dpeaa)DE-He213 Uptake carrier (dpeaa)DE-He213 Export carrier (dpeaa)DE-He213 Xu, Lanlan verfasserin aut Shi, Jianming verfasserin aut Xu, Qingyang verfasserin aut Chen, Ning verfasserin aut Enthalten in Journal of industrial microbiology and biotechnology Berlin : Springer, 1986 39(2012), 10 vom: 26. Juni, Seite 1549-1556 (DE-627)300589514 (DE-600)1482484-X 1476-5535 nnns volume:39 year:2012 number:10 day:26 month:06 pages:1549-1556 https://dx.doi.org/10.1007/s10295-012-1155-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.00 ASE AR 39 2012 10 26 06 1549-1556 |
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Enthalten in Journal of industrial microbiology and biotechnology 39(2012), 10 vom: 26. Juni, Seite 1549-1556 volume:39 year:2012 number:10 day:26 month:06 pages:1549-1556 |
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Xie, Xixian @@aut@@ Xu, Lanlan @@aut@@ Shi, Jianming @@aut@@ Xu, Qingyang @@aut@@ Chen, Ning @@aut@@ |
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2012-06-26T00:00:00Z |
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|
| author |
Xie, Xixian |
| spellingShingle |
Xie, Xixian ddc 570 bkl 42.30 bkl 58.00 misc -isoleucine misc Excretion misc Uptake carrier misc Export carrier Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW |
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Xie, Xixian |
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570 - Life sciences; biology |
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1476-5535 |
| topic_title |
570 ASE 42.30 bkl 58.00 bkl Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW -isoleucine (dpeaa)DE-He213 Excretion (dpeaa)DE-He213 Uptake carrier (dpeaa)DE-He213 Export carrier (dpeaa)DE-He213 |
| topic |
ddc 570 bkl 42.30 bkl 58.00 misc -isoleucine misc Excretion misc Uptake carrier misc Export carrier |
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ddc 570 bkl 42.30 bkl 58.00 misc -isoleucine misc Excretion misc Uptake carrier misc Export carrier |
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ddc 570 bkl 42.30 bkl 58.00 misc -isoleucine misc Excretion misc Uptake carrier misc Export carrier |
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Elektronische Aufsätze Aufsätze Elektronische Ressource |
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Journal of industrial microbiology and biotechnology |
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Journal of industrial microbiology and biotechnology |
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(DE-627)300589514 (DE-600)1482484-X |
| title |
Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW |
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(DE-627)SPR009373039 (SPR)s10295-012-1155-4-e |
| title_full |
Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW |
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Xie, Xixian |
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Journal of industrial microbiology and biotechnology |
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Journal of industrial microbiology and biotechnology |
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eng |
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500 - Science |
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2012 |
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Xie, Xixian Xu, Lanlan Shi, Jianming Xu, Qingyang Chen, Ning |
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39 |
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570 ASE 42.30 bkl 58.00 bkl |
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Elektronische Aufsätze |
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Xie, Xixian |
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10.1007/s10295-012-1155-4 |
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570 |
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verfasserin |
| title_sort |
effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain corynebacterium glutamicum yilw |
| title_auth |
Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW |
| abstract |
Abstract Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in l-isoleucine uptake rates, while overexpression of brnFE leads to enhanced l-isoleucine export rates. Given that net excretion rates would be an important factor for high titers of l-isoleucine accumulation, we have tested the notion that decreased l-isoleucine uptake combined with increased l-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of l-isoleucine. To examine the effect of the two carriers on l-isoleucine accumulation in l-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol $ min^{−1} $ mg (dry wt)−1 and its l-isoleucine titer increased from 154.3 mM (20.2 g $ l^{−1} $) to 170.3 mM (22.3 g $ l^{−1} $). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol $ min^{−1} $ mg (dry wt)−1, and their l-isoleucine production titers were 197.2 mM (25.9 g $ l^{−1} $) and 221.0 mM (29.0 g $ l^{−1} $), respectively. Our results suggest that modifications of the transport system could provide a promising avenue for further increasing l-isoleucine yield in the l-isoleucine producer. |
| abstractGer |
Abstract Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in l-isoleucine uptake rates, while overexpression of brnFE leads to enhanced l-isoleucine export rates. Given that net excretion rates would be an important factor for high titers of l-isoleucine accumulation, we have tested the notion that decreased l-isoleucine uptake combined with increased l-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of l-isoleucine. To examine the effect of the two carriers on l-isoleucine accumulation in l-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol $ min^{−1} $ mg (dry wt)−1 and its l-isoleucine titer increased from 154.3 mM (20.2 g $ l^{−1} $) to 170.3 mM (22.3 g $ l^{−1} $). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol $ min^{−1} $ mg (dry wt)−1, and their l-isoleucine production titers were 197.2 mM (25.9 g $ l^{−1} $) and 221.0 mM (29.0 g $ l^{−1} $), respectively. Our results suggest that modifications of the transport system could provide a promising avenue for further increasing l-isoleucine yield in the l-isoleucine producer. |
| abstract_unstemmed |
Abstract Previous studies have shown that the deletion of brnQ from the Corynebacterium glutamicum chromosome results in a significant reduction in l-isoleucine uptake rates, while overexpression of brnFE leads to enhanced l-isoleucine export rates. Given that net excretion rates would be an important factor for high titers of l-isoleucine accumulation, we have tested the notion that decreased l-isoleucine uptake combined with increased l-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of l-isoleucine. To examine the effect of the two carriers on l-isoleucine accumulation in l-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol $ min^{−1} $ mg (dry wt)−1 and its l-isoleucine titer increased from 154.3 mM (20.2 g $ l^{−1} $) to 170.3 mM (22.3 g $ l^{−1} $). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol $ min^{−1} $ mg (dry wt)−1, and their l-isoleucine production titers were 197.2 mM (25.9 g $ l^{−1} $) and 221.0 mM (29.0 g $ l^{−1} $), respectively. Our results suggest that modifications of the transport system could provide a promising avenue for further increasing l-isoleucine yield in the l-isoleucine producer. |
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| title_short |
Effect of transport proteins on l-isoleucine production with the l-isoleucine-producing strain Corynebacterium glutamicum YILW |
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https://dx.doi.org/10.1007/s10295-012-1155-4 |
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Xu, Lanlan Shi, Jianming Xu, Qingyang Chen, Ning |
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Xu, Lanlan Shi, Jianming Xu, Qingyang Chen, Ning |
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10.1007/s10295-012-1155-4 |
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2024-07-04T01:48:08.072Z |
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1803611210331979776 |
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Given that net excretion rates would be an important factor for high titers of l-isoleucine accumulation, we have tested the notion that decreased l-isoleucine uptake combined with increased l-isoleucine excretion will further improve high-yield strains that are currently used for the industrial-scale production of l-isoleucine. To examine the effect of the two carriers on l-isoleucine accumulation in l-isoleucine producer C. glutamicum YILW, we constructed a brnQ deletion mutant (C. glutamicum YILW∆brnQ) and two brnFE overexpressors (C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE). Compared to the original strain, the efflux rate of the brnQ mutant increased from 19.0 to 23.6 nmol $ min^{−1} $ mg (dry wt)−1 and its l-isoleucine titer increased from 154.3 mM (20.2 g $ l^{−1} $) to 170.3 mM (22.3 g $ l^{−1} $). The efflux rates of C. glutamicum YILWpXMJ19brnFE and C. glutamicum YILW∆brnQpXMJ19brnFE were 33.5 and 39.1 nmol $ min^{−1} $ mg (dry wt)−1, and their l-isoleucine production titers were 197.2 mM (25.9 g $ l^{−1} $) and 221.0 mM (29.0 g $ l^{−1} $), respectively. 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