Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance

Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was compos...
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Autor*in:	Faize, Mohamed [verfasserIn] Faize, Lydia [verfasserIn] Ishii, Hideo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2007

Übergeordnetes Werk:	Enthalten in: Journal of general plant pathology - Tokyo : Springer, 2000, 73(2007), 2 vom: Apr., Seite 104-112
Übergeordnetes Werk:	volume:73 ; year:2007 ; number:2 ; month:04 ; pages:104-112

Links:	Volltext

DOI / URN:	10.1007/s10327-006-0330-8

Katalog-ID:	SPR009438572

Internformat


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100	1		\|a Faize, Mohamed \|e verfasserin \|4 aut
245	1	0	\|a Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance
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520			\|a Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. However, in resistant Kinchaku, expression of this gene was only weakly enhanced. Moreover, treatment with a resistance inducer, acibenzolar-S-methyl (ASM), which significantly reduces scab severity on potted trees of Kousui, was also able to induce and to potentiate the expression of this gene after inoculation. This suggests that the LRPKp gene is possibly involved in ASM-induced resistance.
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773	0	8	\|i Enthalten in \|t Journal of general plant pathology \|d Tokyo : Springer, 2000 \|g 73(2007), 2 vom: Apr., Seite 104-112 \|w (DE-627)362771189 \|w (DE-600)2100156-X \|x 1610-739X \|7 nnns
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912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_267
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
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912			\|a GBV_ILN_4700
936	b	k	\|a 48.54 \|q ASE
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952			\|d 73 \|j 2007 \|e 2 \|c 04 \|h 104-112

Indexfelder

author_variant	m f mf l f lf h i hi
matchkey_str	article:1610739X:2007----::hrceiainfluieiheeteetriertiknslpgnfojpnspaadtp
hierarchy_sort_str	2007
bklnumber	48.54
publishDate	2007
allfields	10.1007/s10327-006-0330-8 doi (DE-627)SPR009438572 (SPR)s10327-006-0330-8-e DE-627 ger DE-627 rakwb eng 630 ASE 580 ASE 48.54 bkl Faize, Mohamed verfasserin aut Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. However, in resistant Kinchaku, expression of this gene was only weakly enhanced. Moreover, treatment with a resistance inducer, acibenzolar-S-methyl (ASM), which significantly reduces scab severity on potted trees of Kousui, was also able to induce and to potentiate the expression of this gene after inoculation. This suggests that the LRPKp gene is possibly involved in ASM-induced resistance. Faize, Lydia verfasserin aut Ishii, Hideo verfasserin aut Enthalten in Journal of general plant pathology Tokyo : Springer, 2000 73(2007), 2 vom: Apr., Seite 104-112 (DE-627)362771189 (DE-600)2100156-X 1610-739X nnns volume:73 year:2007 number:2 month:04 pages:104-112 https://dx.doi.org/10.1007/s10327-006-0330-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 73 2007 2 04 104-112
spelling	10.1007/s10327-006-0330-8 doi (DE-627)SPR009438572 (SPR)s10327-006-0330-8-e DE-627 ger DE-627 rakwb eng 630 ASE 580 ASE 48.54 bkl Faize, Mohamed verfasserin aut Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. However, in resistant Kinchaku, expression of this gene was only weakly enhanced. Moreover, treatment with a resistance inducer, acibenzolar-S-methyl (ASM), which significantly reduces scab severity on potted trees of Kousui, was also able to induce and to potentiate the expression of this gene after inoculation. This suggests that the LRPKp gene is possibly involved in ASM-induced resistance. Faize, Lydia verfasserin aut Ishii, Hideo verfasserin aut Enthalten in Journal of general plant pathology Tokyo : Springer, 2000 73(2007), 2 vom: Apr., Seite 104-112 (DE-627)362771189 (DE-600)2100156-X 1610-739X nnns volume:73 year:2007 number:2 month:04 pages:104-112 https://dx.doi.org/10.1007/s10327-006-0330-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 73 2007 2 04 104-112
allfields_unstemmed	10.1007/s10327-006-0330-8 doi (DE-627)SPR009438572 (SPR)s10327-006-0330-8-e DE-627 ger DE-627 rakwb eng 630 ASE 580 ASE 48.54 bkl Faize, Mohamed verfasserin aut Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. However, in resistant Kinchaku, expression of this gene was only weakly enhanced. Moreover, treatment with a resistance inducer, acibenzolar-S-methyl (ASM), which significantly reduces scab severity on potted trees of Kousui, was also able to induce and to potentiate the expression of this gene after inoculation. This suggests that the LRPKp gene is possibly involved in ASM-induced resistance. Faize, Lydia verfasserin aut Ishii, Hideo verfasserin aut Enthalten in Journal of general plant pathology Tokyo : Springer, 2000 73(2007), 2 vom: Apr., Seite 104-112 (DE-627)362771189 (DE-600)2100156-X 1610-739X nnns volume:73 year:2007 number:2 month:04 pages:104-112 https://dx.doi.org/10.1007/s10327-006-0330-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 73 2007 2 04 104-112
allfieldsGer	10.1007/s10327-006-0330-8 doi (DE-627)SPR009438572 (SPR)s10327-006-0330-8-e DE-627 ger DE-627 rakwb eng 630 ASE 580 ASE 48.54 bkl Faize, Mohamed verfasserin aut Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. However, in resistant Kinchaku, expression of this gene was only weakly enhanced. Moreover, treatment with a resistance inducer, acibenzolar-S-methyl (ASM), which significantly reduces scab severity on potted trees of Kousui, was also able to induce and to potentiate the expression of this gene after inoculation. This suggests that the LRPKp gene is possibly involved in ASM-induced resistance. Faize, Lydia verfasserin aut Ishii, Hideo verfasserin aut Enthalten in Journal of general plant pathology Tokyo : Springer, 2000 73(2007), 2 vom: Apr., Seite 104-112 (DE-627)362771189 (DE-600)2100156-X 1610-739X nnns volume:73 year:2007 number:2 month:04 pages:104-112 https://dx.doi.org/10.1007/s10327-006-0330-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 73 2007 2 04 104-112
allfieldsSound	10.1007/s10327-006-0330-8 doi (DE-627)SPR009438572 (SPR)s10327-006-0330-8-e DE-627 ger DE-627 rakwb eng 630 ASE 580 ASE 48.54 bkl Faize, Mohamed verfasserin aut Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. However, in resistant Kinchaku, expression of this gene was only weakly enhanced. Moreover, treatment with a resistance inducer, acibenzolar-S-methyl (ASM), which significantly reduces scab severity on potted trees of Kousui, was also able to induce and to potentiate the expression of this gene after inoculation. This suggests that the LRPKp gene is possibly involved in ASM-induced resistance. Faize, Lydia verfasserin aut Ishii, Hideo verfasserin aut Enthalten in Journal of general plant pathology Tokyo : Springer, 2000 73(2007), 2 vom: Apr., Seite 104-112 (DE-627)362771189 (DE-600)2100156-X 1610-739X nnns volume:73 year:2007 number:2 month:04 pages:104-112 https://dx.doi.org/10.1007/s10327-006-0330-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 73 2007 2 04 104-112
language	English
source	Enthalten in Journal of general plant pathology 73(2007), 2 vom: Apr., Seite 104-112 volume:73 year:2007 number:2 month:04 pages:104-112
sourceStr	Enthalten in Journal of general plant pathology 73(2007), 2 vom: Apr., Seite 104-112 volume:73 year:2007 number:2 month:04 pages:104-112
format_phy_str_mv	Article
institution	findex.gbv.de
dewey-raw	630
isfreeaccess_bool	false
container_title	Journal of general plant pathology
authorswithroles_txt_mv	Faize, Mohamed @@aut@@ Faize, Lydia @@aut@@ Ishii, Hideo @@aut@@
publishDateDaySort_date	2007-04-01T00:00:00Z
hierarchy_top_id	362771189
dewey-sort	3630
id	SPR009438572
language_de	englisch
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The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. 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author	Faize, Mohamed
spellingShingle	Faize, Mohamed ddc 630 ddc 580 bkl 48.54 Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance
authorStr	Faize, Mohamed
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topic_title	630 ASE 580 ASE 48.54 bkl Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance
topic	ddc 630 ddc 580 bkl 48.54
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title	Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance
ctrlnum	(DE-627)SPR009438572 (SPR)s10327-006-0330-8-e
title_full	Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance
author_sort	Faize, Mohamed
journal	Journal of general plant pathology
journalStr	Journal of general plant pathology
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author_browse	Faize, Mohamed Faize, Lydia Ishii, Hideo
container_volume	73
class	630 ASE 580 ASE 48.54 bkl
format_se	Elektronische Aufsätze
author-letter	Faize, Mohamed
doi_str_mv	10.1007/s10327-006-0330-8
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title_sort	characterization of a leucine-rich repeat receptor-like protein kinase (lrpk) gene from japanese pear and its possible involvement in scab resistance
title_auth	Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance
abstract	Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. However, in resistant Kinchaku, expression of this gene was only weakly enhanced. Moreover, treatment with a resistance inducer, acibenzolar-S-methyl (ASM), which significantly reduces scab severity on potted trees of Kousui, was also able to induce and to potentiate the expression of this gene after inoculation. This suggests that the LRPKp gene is possibly involved in ASM-induced resistance.
abstractGer	Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. However, in resistant Kinchaku, expression of this gene was only weakly enhanced. Moreover, treatment with a resistance inducer, acibenzolar-S-methyl (ASM), which significantly reduces scab severity on potted trees of Kousui, was also able to induce and to potentiate the expression of this gene after inoculation. This suggests that the LRPKp gene is possibly involved in ASM-induced resistance.
abstract_unstemmed	Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. However, in resistant Kinchaku, expression of this gene was only weakly enhanced. Moreover, treatment with a resistance inducer, acibenzolar-S-methyl (ASM), which significantly reduces scab severity on potted trees of Kousui, was also able to induce and to potentiate the expression of this gene after inoculation. This suggests that the LRPKp gene is possibly involved in ASM-induced resistance.
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title_short	Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance
url	https://dx.doi.org/10.1007/s10327-006-0330-8
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author2	Faize, Lydia Ishii, Hideo
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doi_str	10.1007/s10327-006-0330-8
up_date	2024-07-04T02:04:02.583Z
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fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR009438572</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519153424.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201005s2007 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s10327-006-0330-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR009438572</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s10327-006-0330-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">630</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">580</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">48.54</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Faize, Mohamed</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2007</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a transmembrane-spanning segment, and a cytoplasmic kinase domain; it had the highest sequence homology with apple LRPKm and Arabidopsis receptor-like protein kinase (RLK). Southern blot analysis revealed that the LRPKp belongs to a multigenic family, and a polymorphism in restriction fragment length was observed among Asian and European pear cultivars. The LRPKp transcripts were present at a high level in leaves and at a low level in flowers, while no expression was detected in fruits. The expression pattern of LRPKp was also studied by real-time RT-PCR in the scab susceptible cv. Kousui and resistant cvs. Kinchaku and Flemish Beauty after challenge inoculation with Venturia nashicola, the causal agent of Japanese pear scab. Transcripts of LRPKp were transiently enhanced in both the susceptible Kousui and to a great extent in the non-host-resistant Flemish Beauty. However, in resistant Kinchaku, expression of this gene was only weakly enhanced. Moreover, treatment with a resistance inducer, acibenzolar-S-methyl (ASM), which significantly reduces scab severity on potted trees of Kousui, was also able to induce and to potentiate the expression of this gene after inoculation. This suggests that the LRPKp gene is possibly involved in ASM-induced resistance.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Faize, Lydia</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ishii, Hideo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of general plant pathology</subfield><subfield code="d">Tokyo : Springer, 2000</subfield><subfield code="g">73(2007), 2 vom: Apr., Seite 104-112</subfield><subfield code="w">(DE-627)362771189</subfield><subfield code="w">(DE-600)2100156-X</subfield><subfield code="x">1610-739X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:73</subfield><subfield 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Characterization of a leucine-rich repeat receptor-like protein kinase (LRPK) gene from Japanese pear and its possible involvement in scab resistance

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