First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens
Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which...
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Gespeichert in:
| Autor*in: |
Ishii, Hideo [verfasserIn] Tanoue, Junko [verfasserIn] Oshima, Michiyo [verfasserIn] Chung, Wen-Hsin [verfasserIn] Nishimura, Kumiko [verfasserIn] Yamaguchi, Junichiro [verfasserIn] Nemoto, Fumihiro [verfasserIn] So, Kazuhiro [verfasserIn] Iwama, Toshitaka [verfasserIn] Yoshimatsu, Hideaki [verfasserIn] Shimizu, Motoshige [verfasserIn] Kozawa, Toru [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2008 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of general plant pathology - Tokyo : Springer, 2000, 74(2008), 6 vom: 26. Aug., Seite 409-416 |
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| Übergeordnetes Werk: |
volume:74 ; year:2008 ; number:6 ; day:26 ; month:08 ; pages:409-416 |
| Links: |
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| DOI / URN: |
10.1007/s10327-008-0119-z |
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| Katalog-ID: |
SPR009439978 |
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| 520 | |a Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. | ||
| 650 | 4 | |a Fusarium head blight |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a MBI-D fungicides |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Rice blast |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Thiophanate–methyl |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Wheat scab |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Tanoue, Junko |e verfasserin |4 aut | |
| 700 | 1 | |a Oshima, Michiyo |e verfasserin |4 aut | |
| 700 | 1 | |a Chung, Wen-Hsin |e verfasserin |4 aut | |
| 700 | 1 | |a Nishimura, Kumiko |e verfasserin |4 aut | |
| 700 | 1 | |a Yamaguchi, Junichiro |e verfasserin |4 aut | |
| 700 | 1 | |a Nemoto, Fumihiro |e verfasserin |4 aut | |
| 700 | 1 | |a So, Kazuhiro |e verfasserin |4 aut | |
| 700 | 1 | |a Iwama, Toshitaka |e verfasserin |4 aut | |
| 700 | 1 | |a Yoshimatsu, Hideaki |e verfasserin |4 aut | |
| 700 | 1 | |a Shimizu, Motoshige |e verfasserin |4 aut | |
| 700 | 1 | |a Kozawa, Toru |e verfasserin |4 aut | |
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10.1007/s10327-008-0119-z doi (DE-627)SPR009439978 (SPR)s10327-008-0119-z-e DE-627 ger DE-627 rakwb eng 630 ASE 580 ASE 48.54 bkl Ishii, Hideo verfasserin aut First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. Fusarium head blight (dpeaa)DE-He213 MBI-D fungicides (dpeaa)DE-He213 Rice blast (dpeaa)DE-He213 Thiophanate–methyl (dpeaa)DE-He213 Wheat scab (dpeaa)DE-He213 Tanoue, Junko verfasserin aut Oshima, Michiyo verfasserin aut Chung, Wen-Hsin verfasserin aut Nishimura, Kumiko verfasserin aut Yamaguchi, Junichiro verfasserin aut Nemoto, Fumihiro verfasserin aut So, Kazuhiro verfasserin aut Iwama, Toshitaka verfasserin aut Yoshimatsu, Hideaki verfasserin aut Shimizu, Motoshige verfasserin aut Kozawa, Toru verfasserin aut Enthalten in Journal of general plant pathology Tokyo : Springer, 2000 74(2008), 6 vom: 26. Aug., Seite 409-416 (DE-627)362771189 (DE-600)2100156-X 1610-739X nnns volume:74 year:2008 number:6 day:26 month:08 pages:409-416 https://dx.doi.org/10.1007/s10327-008-0119-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 74 2008 6 26 08 409-416 |
| spelling |
10.1007/s10327-008-0119-z doi (DE-627)SPR009439978 (SPR)s10327-008-0119-z-e DE-627 ger DE-627 rakwb eng 630 ASE 580 ASE 48.54 bkl Ishii, Hideo verfasserin aut First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. Fusarium head blight (dpeaa)DE-He213 MBI-D fungicides (dpeaa)DE-He213 Rice blast (dpeaa)DE-He213 Thiophanate–methyl (dpeaa)DE-He213 Wheat scab (dpeaa)DE-He213 Tanoue, Junko verfasserin aut Oshima, Michiyo verfasserin aut Chung, Wen-Hsin verfasserin aut Nishimura, Kumiko verfasserin aut Yamaguchi, Junichiro verfasserin aut Nemoto, Fumihiro verfasserin aut So, Kazuhiro verfasserin aut Iwama, Toshitaka verfasserin aut Yoshimatsu, Hideaki verfasserin aut Shimizu, Motoshige verfasserin aut Kozawa, Toru verfasserin aut Enthalten in Journal of general plant pathology Tokyo : Springer, 2000 74(2008), 6 vom: 26. Aug., Seite 409-416 (DE-627)362771189 (DE-600)2100156-X 1610-739X nnns volume:74 year:2008 number:6 day:26 month:08 pages:409-416 https://dx.doi.org/10.1007/s10327-008-0119-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 74 2008 6 26 08 409-416 |
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10.1007/s10327-008-0119-z doi (DE-627)SPR009439978 (SPR)s10327-008-0119-z-e DE-627 ger DE-627 rakwb eng 630 ASE 580 ASE 48.54 bkl Ishii, Hideo verfasserin aut First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. Fusarium head blight (dpeaa)DE-He213 MBI-D fungicides (dpeaa)DE-He213 Rice blast (dpeaa)DE-He213 Thiophanate–methyl (dpeaa)DE-He213 Wheat scab (dpeaa)DE-He213 Tanoue, Junko verfasserin aut Oshima, Michiyo verfasserin aut Chung, Wen-Hsin verfasserin aut Nishimura, Kumiko verfasserin aut Yamaguchi, Junichiro verfasserin aut Nemoto, Fumihiro verfasserin aut So, Kazuhiro verfasserin aut Iwama, Toshitaka verfasserin aut Yoshimatsu, Hideaki verfasserin aut Shimizu, Motoshige verfasserin aut Kozawa, Toru verfasserin aut Enthalten in Journal of general plant pathology Tokyo : Springer, 2000 74(2008), 6 vom: 26. Aug., Seite 409-416 (DE-627)362771189 (DE-600)2100156-X 1610-739X nnns volume:74 year:2008 number:6 day:26 month:08 pages:409-416 https://dx.doi.org/10.1007/s10327-008-0119-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 74 2008 6 26 08 409-416 |
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10.1007/s10327-008-0119-z doi (DE-627)SPR009439978 (SPR)s10327-008-0119-z-e DE-627 ger DE-627 rakwb eng 630 ASE 580 ASE 48.54 bkl Ishii, Hideo verfasserin aut First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. Fusarium head blight (dpeaa)DE-He213 MBI-D fungicides (dpeaa)DE-He213 Rice blast (dpeaa)DE-He213 Thiophanate–methyl (dpeaa)DE-He213 Wheat scab (dpeaa)DE-He213 Tanoue, Junko verfasserin aut Oshima, Michiyo verfasserin aut Chung, Wen-Hsin verfasserin aut Nishimura, Kumiko verfasserin aut Yamaguchi, Junichiro verfasserin aut Nemoto, Fumihiro verfasserin aut So, Kazuhiro verfasserin aut Iwama, Toshitaka verfasserin aut Yoshimatsu, Hideaki verfasserin aut Shimizu, Motoshige verfasserin aut Kozawa, Toru verfasserin aut Enthalten in Journal of general plant pathology Tokyo : Springer, 2000 74(2008), 6 vom: 26. Aug., Seite 409-416 (DE-627)362771189 (DE-600)2100156-X 1610-739X nnns volume:74 year:2008 number:6 day:26 month:08 pages:409-416 https://dx.doi.org/10.1007/s10327-008-0119-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 74 2008 6 26 08 409-416 |
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10.1007/s10327-008-0119-z doi (DE-627)SPR009439978 (SPR)s10327-008-0119-z-e DE-627 ger DE-627 rakwb eng 630 ASE 580 ASE 48.54 bkl Ishii, Hideo verfasserin aut First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. Fusarium head blight (dpeaa)DE-He213 MBI-D fungicides (dpeaa)DE-He213 Rice blast (dpeaa)DE-He213 Thiophanate–methyl (dpeaa)DE-He213 Wheat scab (dpeaa)DE-He213 Tanoue, Junko verfasserin aut Oshima, Michiyo verfasserin aut Chung, Wen-Hsin verfasserin aut Nishimura, Kumiko verfasserin aut Yamaguchi, Junichiro verfasserin aut Nemoto, Fumihiro verfasserin aut So, Kazuhiro verfasserin aut Iwama, Toshitaka verfasserin aut Yoshimatsu, Hideaki verfasserin aut Shimizu, Motoshige verfasserin aut Kozawa, Toru verfasserin aut Enthalten in Journal of general plant pathology Tokyo : Springer, 2000 74(2008), 6 vom: 26. Aug., Seite 409-416 (DE-627)362771189 (DE-600)2100156-X 1610-739X nnns volume:74 year:2008 number:6 day:26 month:08 pages:409-416 https://dx.doi.org/10.1007/s10327-008-0119-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 74 2008 6 26 08 409-416 |
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Enthalten in Journal of general plant pathology 74(2008), 6 vom: 26. Aug., Seite 409-416 volume:74 year:2008 number:6 day:26 month:08 pages:409-416 |
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Enthalten in Journal of general plant pathology 74(2008), 6 vom: 26. Aug., Seite 409-416 volume:74 year:2008 number:6 day:26 month:08 pages:409-416 |
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Fusarium head blight MBI-D fungicides Rice blast Thiophanate–methyl Wheat scab |
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Journal of general plant pathology |
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Ishii, Hideo @@aut@@ Tanoue, Junko @@aut@@ Oshima, Michiyo @@aut@@ Chung, Wen-Hsin @@aut@@ Nishimura, Kumiko @@aut@@ Yamaguchi, Junichiro @@aut@@ Nemoto, Fumihiro @@aut@@ So, Kazuhiro @@aut@@ Iwama, Toshitaka @@aut@@ Yoshimatsu, Hideaki @@aut@@ Shimizu, Motoshige @@aut@@ Kozawa, Toru @@aut@@ |
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2008-08-26T00:00:00Z |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR009439978</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519153426.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201005s2008 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s10327-008-0119-z</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR009439978</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s10327-008-0119-z-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">630</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">580</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">48.54</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Ishii, Hideo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2008</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. 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Ishii, Hideo |
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Ishii, Hideo ddc 630 ddc 580 bkl 48.54 misc Fusarium head blight misc MBI-D fungicides misc Rice blast misc Thiophanate–methyl misc Wheat scab First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens |
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630 ASE 580 ASE 48.54 bkl First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens Fusarium head blight (dpeaa)DE-He213 MBI-D fungicides (dpeaa)DE-He213 Rice blast (dpeaa)DE-He213 Thiophanate–methyl (dpeaa)DE-He213 Wheat scab (dpeaa)DE-He213 |
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ddc 630 ddc 580 bkl 48.54 misc Fusarium head blight misc MBI-D fungicides misc Rice blast misc Thiophanate–methyl misc Wheat scab |
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ddc 630 ddc 580 bkl 48.54 misc Fusarium head blight misc MBI-D fungicides misc Rice blast misc Thiophanate–methyl misc Wheat scab |
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ddc 630 ddc 580 bkl 48.54 misc Fusarium head blight misc MBI-D fungicides misc Rice blast misc Thiophanate–methyl misc Wheat scab |
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First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens |
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(DE-627)SPR009439978 (SPR)s10327-008-0119-z-e |
| title_full |
First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens |
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Ishii, Hideo |
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Journal of general plant pathology |
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Journal of general plant pathology |
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eng |
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600 - Technology 500 - Science |
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2008 |
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Ishii, Hideo Tanoue, Junko Oshima, Michiyo Chung, Wen-Hsin Nishimura, Kumiko Yamaguchi, Junichiro Nemoto, Fumihiro So, Kazuhiro Iwama, Toshitaka Yoshimatsu, Hideaki Shimizu, Motoshige Kozawa, Toru |
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Elektronische Aufsätze |
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Ishii, Hideo |
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10.1007/s10327-008-0119-z |
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630 580 |
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verfasserin |
| title_sort |
first application of pcr-luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens |
| title_auth |
First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens |
| abstract |
Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. |
| abstractGer |
Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. |
| abstract_unstemmed |
Abstract Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. |
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6 |
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First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens |
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https://dx.doi.org/10.1007/s10327-008-0119-z |
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Tanoue, Junko Oshima, Michiyo Chung, Wen-Hsin Nishimura, Kumiko Yamaguchi, Junichiro Nemoto, Fumihiro So, Kazuhiro Iwama, Toshitaka Yoshimatsu, Hideaki Shimizu, Motoshige Kozawa, Toru |
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Tanoue, Junko Oshima, Michiyo Chung, Wen-Hsin Nishimura, Kumiko Yamaguchi, Junichiro Nemoto, Fumihiro So, Kazuhiro Iwama, Toshitaka Yoshimatsu, Hideaki Shimizu, Motoshige Kozawa, Toru |
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| score |
7.398904 |