Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase

Abstract Mixed-mode HPLC (MM-HPLC), combining different interactions or retention modes in a single column, can be an interesting alternative to reversed-phase HPLC, notably to achieve the combined retention of polar and non-polar species. In the present fundamental study, we have selected one bimod...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Lemasson, Elise [verfasserIn] Richer, Yoann [verfasserIn] Bertin, Sophie [verfasserIn] Hennig, Philippe [verfasserIn] West, Caroline [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Reversed-phase liquid chromatography Mixed-mode chromatography Linear solvation energy relationships (LSER) Solvation parameter model Stationary phase characterization Thermodynamic characterization

Übergeordnetes Werk:	Enthalten in: Chromatographia - Wiesbaden : Vieweg, 1968, 81(2018), 3 vom: 25. Jan., Seite 387-399
Übergeordnetes Werk:	volume:81 ; year:2018 ; number:3 ; day:25 ; month:01 ; pages:387-399

Links:	Volltext

DOI / URN:	10.1007/s10337-018-3477-5

Katalog-ID:	SPR009569553

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520			\|a Abstract Mixed-mode HPLC (MM-HPLC), combining different interactions or retention modes in a single column, can be an interesting alternative to reversed-phase HPLC, notably to achieve the combined retention of polar and non-polar species. In the present fundamental study, we have selected one bimodal stationary phase allowing for both reversed-phase and weak cation-exchange retention modes (Acclaim mixed-mode WCX-1 LC). First, the mobile phase buffer composition (buffer pH ranging from 5 to 7 and concentration ranging from 20 to 100 mM) was explored with a small set of probe compounds (15 acids, bases and neutrals) to ensure adequate retention and peak shapes for the target compounds, and to evaluate the relative contributions of reversed-phase and ion-exchange mechanisms. Second, retention values measured for 63 probe compounds with various proportions of acetonitrile (ranging from 30 to 80%) served to establish linear solvation energy relationships based on (a) the usual and (b) a modified version of the solvation parameter model comprising additional descriptors to take account of interactions with ionizable species to bring some insights into the retention mechanisms. Finally, temperature effects at the low (30%) and high (60%) proportions of acetonitrile were observed between 20 and 40 °C (with 5 °C increments) and Van’t Hoff plots were drawn to measure the changes in interactions energies when the mobile phase composition changed. Graphical Abstract
650		4	\|a Reversed-phase liquid chromatography \|7 (dpeaa)DE-He213
650		4	\|a Mixed-mode chromatography \|7 (dpeaa)DE-He213
650		4	\|a Linear solvation energy relationships (LSER) \|7 (dpeaa)DE-He213
650		4	\|a Solvation parameter model \|7 (dpeaa)DE-He213
650		4	\|a Stationary phase characterization \|7 (dpeaa)DE-He213
650		4	\|a Thermodynamic characterization \|7 (dpeaa)DE-He213
700	1		\|a Richer, Yoann \|e verfasserin \|4 aut
700	1		\|a Bertin, Sophie \|e verfasserin \|4 aut
700	1		\|a Hennig, Philippe \|e verfasserin \|4 aut
700	1		\|a West, Caroline \|e verfasserin \|4 aut
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912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_267
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
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912			\|a GBV_ILN_2147
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912			\|a GBV_ILN_2152
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912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
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912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
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912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
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952			\|d 81 \|j 2018 \|e 3 \|b 25 \|c 01 \|h 387-399

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author_variant	e l el y r yr s b sb p h ph c w cw
matchkey_str	article:16121112:2018----::hrceiainfeetomcaimimxdoepcihbmdleesdhsc
hierarchy_sort_str	2018
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publishDate	2018
allfields	10.1007/s10337-018-3477-5 doi (DE-627)SPR009569553 (SPR)s10337-018-3477-5-e DE-627 ger DE-627 rakwb eng 540 ASE 540 ASE 35.29 bkl Lemasson, Elise verfasserin aut Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Mixed-mode HPLC (MM-HPLC), combining different interactions or retention modes in a single column, can be an interesting alternative to reversed-phase HPLC, notably to achieve the combined retention of polar and non-polar species. In the present fundamental study, we have selected one bimodal stationary phase allowing for both reversed-phase and weak cation-exchange retention modes (Acclaim mixed-mode WCX-1 LC). First, the mobile phase buffer composition (buffer pH ranging from 5 to 7 and concentration ranging from 20 to 100 mM) was explored with a small set of probe compounds (15 acids, bases and neutrals) to ensure adequate retention and peak shapes for the target compounds, and to evaluate the relative contributions of reversed-phase and ion-exchange mechanisms. Second, retention values measured for 63 probe compounds with various proportions of acetonitrile (ranging from 30 to 80%) served to establish linear solvation energy relationships based on (a) the usual and (b) a modified version of the solvation parameter model comprising additional descriptors to take account of interactions with ionizable species to bring some insights into the retention mechanisms. Finally, temperature effects at the low (30%) and high (60%) proportions of acetonitrile were observed between 20 and 40 °C (with 5 °C increments) and Van’t Hoff plots were drawn to measure the changes in interactions energies when the mobile phase composition changed. Graphical Abstract Reversed-phase liquid chromatography (dpeaa)DE-He213 Mixed-mode chromatography (dpeaa)DE-He213 Linear solvation energy relationships (LSER) (dpeaa)DE-He213 Solvation parameter model (dpeaa)DE-He213 Stationary phase characterization (dpeaa)DE-He213 Thermodynamic characterization (dpeaa)DE-He213 Richer, Yoann verfasserin aut Bertin, Sophie verfasserin aut Hennig, Philippe verfasserin aut West, Caroline verfasserin aut Enthalten in Chromatographia Wiesbaden : Vieweg, 1968 81(2018), 3 vom: 25. Jan., Seite 387-399 (DE-627)320592219 (DE-600)2019091-8 1612-1112 nnns volume:81 year:2018 number:3 day:25 month:01 pages:387-399 https://dx.doi.org/10.1007/s10337-018-3477-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.29 ASE AR 81 2018 3 25 01 387-399
spelling	10.1007/s10337-018-3477-5 doi (DE-627)SPR009569553 (SPR)s10337-018-3477-5-e DE-627 ger DE-627 rakwb eng 540 ASE 540 ASE 35.29 bkl Lemasson, Elise verfasserin aut Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Mixed-mode HPLC (MM-HPLC), combining different interactions or retention modes in a single column, can be an interesting alternative to reversed-phase HPLC, notably to achieve the combined retention of polar and non-polar species. In the present fundamental study, we have selected one bimodal stationary phase allowing for both reversed-phase and weak cation-exchange retention modes (Acclaim mixed-mode WCX-1 LC). First, the mobile phase buffer composition (buffer pH ranging from 5 to 7 and concentration ranging from 20 to 100 mM) was explored with a small set of probe compounds (15 acids, bases and neutrals) to ensure adequate retention and peak shapes for the target compounds, and to evaluate the relative contributions of reversed-phase and ion-exchange mechanisms. Second, retention values measured for 63 probe compounds with various proportions of acetonitrile (ranging from 30 to 80%) served to establish linear solvation energy relationships based on (a) the usual and (b) a modified version of the solvation parameter model comprising additional descriptors to take account of interactions with ionizable species to bring some insights into the retention mechanisms. Finally, temperature effects at the low (30%) and high (60%) proportions of acetonitrile were observed between 20 and 40 °C (with 5 °C increments) and Van’t Hoff plots were drawn to measure the changes in interactions energies when the mobile phase composition changed. Graphical Abstract Reversed-phase liquid chromatography (dpeaa)DE-He213 Mixed-mode chromatography (dpeaa)DE-He213 Linear solvation energy relationships (LSER) (dpeaa)DE-He213 Solvation parameter model (dpeaa)DE-He213 Stationary phase characterization (dpeaa)DE-He213 Thermodynamic characterization (dpeaa)DE-He213 Richer, Yoann verfasserin aut Bertin, Sophie verfasserin aut Hennig, Philippe verfasserin aut West, Caroline verfasserin aut Enthalten in Chromatographia Wiesbaden : Vieweg, 1968 81(2018), 3 vom: 25. Jan., Seite 387-399 (DE-627)320592219 (DE-600)2019091-8 1612-1112 nnns volume:81 year:2018 number:3 day:25 month:01 pages:387-399 https://dx.doi.org/10.1007/s10337-018-3477-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.29 ASE AR 81 2018 3 25 01 387-399
allfields_unstemmed	10.1007/s10337-018-3477-5 doi (DE-627)SPR009569553 (SPR)s10337-018-3477-5-e DE-627 ger DE-627 rakwb eng 540 ASE 540 ASE 35.29 bkl Lemasson, Elise verfasserin aut Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Mixed-mode HPLC (MM-HPLC), combining different interactions or retention modes in a single column, can be an interesting alternative to reversed-phase HPLC, notably to achieve the combined retention of polar and non-polar species. In the present fundamental study, we have selected one bimodal stationary phase allowing for both reversed-phase and weak cation-exchange retention modes (Acclaim mixed-mode WCX-1 LC). First, the mobile phase buffer composition (buffer pH ranging from 5 to 7 and concentration ranging from 20 to 100 mM) was explored with a small set of probe compounds (15 acids, bases and neutrals) to ensure adequate retention and peak shapes for the target compounds, and to evaluate the relative contributions of reversed-phase and ion-exchange mechanisms. Second, retention values measured for 63 probe compounds with various proportions of acetonitrile (ranging from 30 to 80%) served to establish linear solvation energy relationships based on (a) the usual and (b) a modified version of the solvation parameter model comprising additional descriptors to take account of interactions with ionizable species to bring some insights into the retention mechanisms. Finally, temperature effects at the low (30%) and high (60%) proportions of acetonitrile were observed between 20 and 40 °C (with 5 °C increments) and Van’t Hoff plots were drawn to measure the changes in interactions energies when the mobile phase composition changed. Graphical Abstract Reversed-phase liquid chromatography (dpeaa)DE-He213 Mixed-mode chromatography (dpeaa)DE-He213 Linear solvation energy relationships (LSER) (dpeaa)DE-He213 Solvation parameter model (dpeaa)DE-He213 Stationary phase characterization (dpeaa)DE-He213 Thermodynamic characterization (dpeaa)DE-He213 Richer, Yoann verfasserin aut Bertin, Sophie verfasserin aut Hennig, Philippe verfasserin aut West, Caroline verfasserin aut Enthalten in Chromatographia Wiesbaden : Vieweg, 1968 81(2018), 3 vom: 25. Jan., Seite 387-399 (DE-627)320592219 (DE-600)2019091-8 1612-1112 nnns volume:81 year:2018 number:3 day:25 month:01 pages:387-399 https://dx.doi.org/10.1007/s10337-018-3477-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.29 ASE AR 81 2018 3 25 01 387-399
allfieldsGer	10.1007/s10337-018-3477-5 doi (DE-627)SPR009569553 (SPR)s10337-018-3477-5-e DE-627 ger DE-627 rakwb eng 540 ASE 540 ASE 35.29 bkl Lemasson, Elise verfasserin aut Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Mixed-mode HPLC (MM-HPLC), combining different interactions or retention modes in a single column, can be an interesting alternative to reversed-phase HPLC, notably to achieve the combined retention of polar and non-polar species. In the present fundamental study, we have selected one bimodal stationary phase allowing for both reversed-phase and weak cation-exchange retention modes (Acclaim mixed-mode WCX-1 LC). First, the mobile phase buffer composition (buffer pH ranging from 5 to 7 and concentration ranging from 20 to 100 mM) was explored with a small set of probe compounds (15 acids, bases and neutrals) to ensure adequate retention and peak shapes for the target compounds, and to evaluate the relative contributions of reversed-phase and ion-exchange mechanisms. Second, retention values measured for 63 probe compounds with various proportions of acetonitrile (ranging from 30 to 80%) served to establish linear solvation energy relationships based on (a) the usual and (b) a modified version of the solvation parameter model comprising additional descriptors to take account of interactions with ionizable species to bring some insights into the retention mechanisms. Finally, temperature effects at the low (30%) and high (60%) proportions of acetonitrile were observed between 20 and 40 °C (with 5 °C increments) and Van’t Hoff plots were drawn to measure the changes in interactions energies when the mobile phase composition changed. Graphical Abstract Reversed-phase liquid chromatography (dpeaa)DE-He213 Mixed-mode chromatography (dpeaa)DE-He213 Linear solvation energy relationships (LSER) (dpeaa)DE-He213 Solvation parameter model (dpeaa)DE-He213 Stationary phase characterization (dpeaa)DE-He213 Thermodynamic characterization (dpeaa)DE-He213 Richer, Yoann verfasserin aut Bertin, Sophie verfasserin aut Hennig, Philippe verfasserin aut West, Caroline verfasserin aut Enthalten in Chromatographia Wiesbaden : Vieweg, 1968 81(2018), 3 vom: 25. Jan., Seite 387-399 (DE-627)320592219 (DE-600)2019091-8 1612-1112 nnns volume:81 year:2018 number:3 day:25 month:01 pages:387-399 https://dx.doi.org/10.1007/s10337-018-3477-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.29 ASE AR 81 2018 3 25 01 387-399
allfieldsSound	10.1007/s10337-018-3477-5 doi (DE-627)SPR009569553 (SPR)s10337-018-3477-5-e DE-627 ger DE-627 rakwb eng 540 ASE 540 ASE 35.29 bkl Lemasson, Elise verfasserin aut Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Mixed-mode HPLC (MM-HPLC), combining different interactions or retention modes in a single column, can be an interesting alternative to reversed-phase HPLC, notably to achieve the combined retention of polar and non-polar species. In the present fundamental study, we have selected one bimodal stationary phase allowing for both reversed-phase and weak cation-exchange retention modes (Acclaim mixed-mode WCX-1 LC). First, the mobile phase buffer composition (buffer pH ranging from 5 to 7 and concentration ranging from 20 to 100 mM) was explored with a small set of probe compounds (15 acids, bases and neutrals) to ensure adequate retention and peak shapes for the target compounds, and to evaluate the relative contributions of reversed-phase and ion-exchange mechanisms. Second, retention values measured for 63 probe compounds with various proportions of acetonitrile (ranging from 30 to 80%) served to establish linear solvation energy relationships based on (a) the usual and (b) a modified version of the solvation parameter model comprising additional descriptors to take account of interactions with ionizable species to bring some insights into the retention mechanisms. Finally, temperature effects at the low (30%) and high (60%) proportions of acetonitrile were observed between 20 and 40 °C (with 5 °C increments) and Van’t Hoff plots were drawn to measure the changes in interactions energies when the mobile phase composition changed. Graphical Abstract Reversed-phase liquid chromatography (dpeaa)DE-He213 Mixed-mode chromatography (dpeaa)DE-He213 Linear solvation energy relationships (LSER) (dpeaa)DE-He213 Solvation parameter model (dpeaa)DE-He213 Stationary phase characterization (dpeaa)DE-He213 Thermodynamic characterization (dpeaa)DE-He213 Richer, Yoann verfasserin aut Bertin, Sophie verfasserin aut Hennig, Philippe verfasserin aut West, Caroline verfasserin aut Enthalten in Chromatographia Wiesbaden : Vieweg, 1968 81(2018), 3 vom: 25. Jan., Seite 387-399 (DE-627)320592219 (DE-600)2019091-8 1612-1112 nnns volume:81 year:2018 number:3 day:25 month:01 pages:387-399 https://dx.doi.org/10.1007/s10337-018-3477-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.29 ASE AR 81 2018 3 25 01 387-399
language	English
source	Enthalten in Chromatographia 81(2018), 3 vom: 25. Jan., Seite 387-399 volume:81 year:2018 number:3 day:25 month:01 pages:387-399
sourceStr	Enthalten in Chromatographia 81(2018), 3 vom: 25. Jan., Seite 387-399 volume:81 year:2018 number:3 day:25 month:01 pages:387-399
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Reversed-phase liquid chromatography Mixed-mode chromatography Linear solvation energy relationships (LSER) Solvation parameter model Stationary phase characterization Thermodynamic characterization
dewey-raw	540
isfreeaccess_bool	false
container_title	Chromatographia
authorswithroles_txt_mv	Lemasson, Elise @@aut@@ Richer, Yoann @@aut@@ Bertin, Sophie @@aut@@ Hennig, Philippe @@aut@@ West, Caroline @@aut@@
publishDateDaySort_date	2018-01-25T00:00:00Z
hierarchy_top_id	320592219
dewey-sort	3540
id	SPR009569553
language_de	englisch
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author	Lemasson, Elise
spellingShingle	Lemasson, Elise ddc 540 bkl 35.29 misc Reversed-phase liquid chromatography misc Mixed-mode chromatography misc Linear solvation energy relationships (LSER) misc Solvation parameter model misc Stationary phase characterization misc Thermodynamic characterization Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase
authorStr	Lemasson, Elise
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topic_title	540 ASE 35.29 bkl Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase Reversed-phase liquid chromatography (dpeaa)DE-He213 Mixed-mode chromatography (dpeaa)DE-He213 Linear solvation energy relationships (LSER) (dpeaa)DE-He213 Solvation parameter model (dpeaa)DE-He213 Stationary phase characterization (dpeaa)DE-He213 Thermodynamic characterization (dpeaa)DE-He213
topic	ddc 540 bkl 35.29 misc Reversed-phase liquid chromatography misc Mixed-mode chromatography misc Linear solvation energy relationships (LSER) misc Solvation parameter model misc Stationary phase characterization misc Thermodynamic characterization
topic_unstemmed	ddc 540 bkl 35.29 misc Reversed-phase liquid chromatography misc Mixed-mode chromatography misc Linear solvation energy relationships (LSER) misc Solvation parameter model misc Stationary phase characterization misc Thermodynamic characterization
topic_browse	ddc 540 bkl 35.29 misc Reversed-phase liquid chromatography misc Mixed-mode chromatography misc Linear solvation energy relationships (LSER) misc Solvation parameter model misc Stationary phase characterization misc Thermodynamic characterization
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title	Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase
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title_full	Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase
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author_browse	Lemasson, Elise Richer, Yoann Bertin, Sophie Hennig, Philippe West, Caroline
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doi_str_mv	10.1007/s10337-018-3477-5
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title_sort	characterization of retention mechanisms in mixed-mode hplc with a bimodal reversed-phase/cation-exchange stationary phase
title_auth	Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase
abstract	Abstract Mixed-mode HPLC (MM-HPLC), combining different interactions or retention modes in a single column, can be an interesting alternative to reversed-phase HPLC, notably to achieve the combined retention of polar and non-polar species. In the present fundamental study, we have selected one bimodal stationary phase allowing for both reversed-phase and weak cation-exchange retention modes (Acclaim mixed-mode WCX-1 LC). First, the mobile phase buffer composition (buffer pH ranging from 5 to 7 and concentration ranging from 20 to 100 mM) was explored with a small set of probe compounds (15 acids, bases and neutrals) to ensure adequate retention and peak shapes for the target compounds, and to evaluate the relative contributions of reversed-phase and ion-exchange mechanisms. Second, retention values measured for 63 probe compounds with various proportions of acetonitrile (ranging from 30 to 80%) served to establish linear solvation energy relationships based on (a) the usual and (b) a modified version of the solvation parameter model comprising additional descriptors to take account of interactions with ionizable species to bring some insights into the retention mechanisms. Finally, temperature effects at the low (30%) and high (60%) proportions of acetonitrile were observed between 20 and 40 °C (with 5 °C increments) and Van’t Hoff plots were drawn to measure the changes in interactions energies when the mobile phase composition changed. Graphical Abstract
abstractGer	Abstract Mixed-mode HPLC (MM-HPLC), combining different interactions or retention modes in a single column, can be an interesting alternative to reversed-phase HPLC, notably to achieve the combined retention of polar and non-polar species. In the present fundamental study, we have selected one bimodal stationary phase allowing for both reversed-phase and weak cation-exchange retention modes (Acclaim mixed-mode WCX-1 LC). First, the mobile phase buffer composition (buffer pH ranging from 5 to 7 and concentration ranging from 20 to 100 mM) was explored with a small set of probe compounds (15 acids, bases and neutrals) to ensure adequate retention and peak shapes for the target compounds, and to evaluate the relative contributions of reversed-phase and ion-exchange mechanisms. Second, retention values measured for 63 probe compounds with various proportions of acetonitrile (ranging from 30 to 80%) served to establish linear solvation energy relationships based on (a) the usual and (b) a modified version of the solvation parameter model comprising additional descriptors to take account of interactions with ionizable species to bring some insights into the retention mechanisms. Finally, temperature effects at the low (30%) and high (60%) proportions of acetonitrile were observed between 20 and 40 °C (with 5 °C increments) and Van’t Hoff plots were drawn to measure the changes in interactions energies when the mobile phase composition changed. Graphical Abstract
abstract_unstemmed	Abstract Mixed-mode HPLC (MM-HPLC), combining different interactions or retention modes in a single column, can be an interesting alternative to reversed-phase HPLC, notably to achieve the combined retention of polar and non-polar species. In the present fundamental study, we have selected one bimodal stationary phase allowing for both reversed-phase and weak cation-exchange retention modes (Acclaim mixed-mode WCX-1 LC). First, the mobile phase buffer composition (buffer pH ranging from 5 to 7 and concentration ranging from 20 to 100 mM) was explored with a small set of probe compounds (15 acids, bases and neutrals) to ensure adequate retention and peak shapes for the target compounds, and to evaluate the relative contributions of reversed-phase and ion-exchange mechanisms. Second, retention values measured for 63 probe compounds with various proportions of acetonitrile (ranging from 30 to 80%) served to establish linear solvation energy relationships based on (a) the usual and (b) a modified version of the solvation parameter model comprising additional descriptors to take account of interactions with ionizable species to bring some insights into the retention mechanisms. Finally, temperature effects at the low (30%) and high (60%) proportions of acetonitrile were observed between 20 and 40 °C (with 5 °C increments) and Van’t Hoff plots were drawn to measure the changes in interactions energies when the mobile phase composition changed. Graphical Abstract
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title_short	Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase
url	https://dx.doi.org/10.1007/s10337-018-3477-5
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author2	Richer, Yoann Bertin, Sophie Hennig, Philippe West, Caroline
author2Str	Richer, Yoann Bertin, Sophie Hennig, Philippe West, Caroline
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doi_str	10.1007/s10337-018-3477-5
up_date	2024-07-04T02:33:34.830Z
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Characterization of Retention Mechanisms in Mixed-Mode HPLC with a Bimodal Reversed-Phase/Cation-Exchange Stationary Phase

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