Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment
Abstract A novel mesophilic, methylotrophic, methanogenic archaeon, designated strain $ EK1^{T} $, was enriched and isolated from wetland sediment. Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Cha, In-Tae [verfasserIn] Min, Ui-Gi [verfasserIn] Kim, So-Jeong [verfasserIn] Yim, Kyung June [verfasserIn] Roh, Seong Woon [verfasserIn] Rhee, Sung-Keun [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Antonie van Leeuwenhoek - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1934, 104(2013), 6 vom: 03. Sept., Seite 1005-1012 |
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| Übergeordnetes Werk: |
volume:104 ; year:2013 ; number:6 ; day:03 ; month:09 ; pages:1005-1012 |
| Links: |
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| DOI / URN: |
10.1007/s10482-013-0020-4 |
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| Katalog-ID: |
SPR010373624 |
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| 245 | 1 | 0 | |a Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment |
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| 520 | |a Abstract A novel mesophilic, methylotrophic, methanogenic archaeon, designated strain $ EK1^{T} $, was enriched and isolated from wetland sediment. Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. The type strain is strain $ EK1^{T} $(=NBRC $ 109636^{T} $ = KCTC $ 4119^{T} $ = JCM $ 19217^{T} $). | ||
| 650 | 4 | |a Methanogen |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Polyphasic taxonomy |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Wetland sediment |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Min, Ui-Gi |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, So-Jeong |e verfasserin |4 aut | |
| 700 | 1 | |a Yim, Kyung June |e verfasserin |4 aut | |
| 700 | 1 | |a Roh, Seong Woon |e verfasserin |4 aut | |
| 700 | 1 | |a Rhee, Sung-Keun |e verfasserin |4 aut | |
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10.1007/s10482-013-0020-4 doi (DE-627)SPR010373624 (SPR)s10482-013-0020-4-e DE-627 ger DE-627 rakwb eng 570 610 ASE 42.30 bkl Cha, In-Tae verfasserin aut Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A novel mesophilic, methylotrophic, methanogenic archaeon, designated strain $ EK1^{T} $, was enriched and isolated from wetland sediment. Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. The type strain is strain $ EK1^{T} $(=NBRC $ 109636^{T} $ = KCTC $ 4119^{T} $ = JCM $ 19217^{T} $). Methanogen (dpeaa)DE-He213 Polyphasic taxonomy (dpeaa)DE-He213 Wetland sediment (dpeaa)DE-He213 Min, Ui-Gi verfasserin aut Kim, So-Jeong verfasserin aut Yim, Kyung June verfasserin aut Roh, Seong Woon verfasserin aut Rhee, Sung-Keun verfasserin aut Enthalten in Antonie van Leeuwenhoek Dordrecht [u.a.] : Springer Science + Business Media B.V, 1934 104(2013), 6 vom: 03. Sept., Seite 1005-1012 (DE-627)270929355 (DE-600)1478112-8 1572-9699 nnns volume:104 year:2013 number:6 day:03 month:09 pages:1005-1012 https://dx.doi.org/10.1007/s10482-013-0020-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE AR 104 2013 6 03 09 1005-1012 |
| spelling |
10.1007/s10482-013-0020-4 doi (DE-627)SPR010373624 (SPR)s10482-013-0020-4-e DE-627 ger DE-627 rakwb eng 570 610 ASE 42.30 bkl Cha, In-Tae verfasserin aut Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A novel mesophilic, methylotrophic, methanogenic archaeon, designated strain $ EK1^{T} $, was enriched and isolated from wetland sediment. Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. The type strain is strain $ EK1^{T} $(=NBRC $ 109636^{T} $ = KCTC $ 4119^{T} $ = JCM $ 19217^{T} $). Methanogen (dpeaa)DE-He213 Polyphasic taxonomy (dpeaa)DE-He213 Wetland sediment (dpeaa)DE-He213 Min, Ui-Gi verfasserin aut Kim, So-Jeong verfasserin aut Yim, Kyung June verfasserin aut Roh, Seong Woon verfasserin aut Rhee, Sung-Keun verfasserin aut Enthalten in Antonie van Leeuwenhoek Dordrecht [u.a.] : Springer Science + Business Media B.V, 1934 104(2013), 6 vom: 03. Sept., Seite 1005-1012 (DE-627)270929355 (DE-600)1478112-8 1572-9699 nnns volume:104 year:2013 number:6 day:03 month:09 pages:1005-1012 https://dx.doi.org/10.1007/s10482-013-0020-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE AR 104 2013 6 03 09 1005-1012 |
| allfields_unstemmed |
10.1007/s10482-013-0020-4 doi (DE-627)SPR010373624 (SPR)s10482-013-0020-4-e DE-627 ger DE-627 rakwb eng 570 610 ASE 42.30 bkl Cha, In-Tae verfasserin aut Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A novel mesophilic, methylotrophic, methanogenic archaeon, designated strain $ EK1^{T} $, was enriched and isolated from wetland sediment. Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. The type strain is strain $ EK1^{T} $(=NBRC $ 109636^{T} $ = KCTC $ 4119^{T} $ = JCM $ 19217^{T} $). Methanogen (dpeaa)DE-He213 Polyphasic taxonomy (dpeaa)DE-He213 Wetland sediment (dpeaa)DE-He213 Min, Ui-Gi verfasserin aut Kim, So-Jeong verfasserin aut Yim, Kyung June verfasserin aut Roh, Seong Woon verfasserin aut Rhee, Sung-Keun verfasserin aut Enthalten in Antonie van Leeuwenhoek Dordrecht [u.a.] : Springer Science + Business Media B.V, 1934 104(2013), 6 vom: 03. Sept., Seite 1005-1012 (DE-627)270929355 (DE-600)1478112-8 1572-9699 nnns volume:104 year:2013 number:6 day:03 month:09 pages:1005-1012 https://dx.doi.org/10.1007/s10482-013-0020-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE AR 104 2013 6 03 09 1005-1012 |
| allfieldsGer |
10.1007/s10482-013-0020-4 doi (DE-627)SPR010373624 (SPR)s10482-013-0020-4-e DE-627 ger DE-627 rakwb eng 570 610 ASE 42.30 bkl Cha, In-Tae verfasserin aut Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A novel mesophilic, methylotrophic, methanogenic archaeon, designated strain $ EK1^{T} $, was enriched and isolated from wetland sediment. Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. The type strain is strain $ EK1^{T} $(=NBRC $ 109636^{T} $ = KCTC $ 4119^{T} $ = JCM $ 19217^{T} $). Methanogen (dpeaa)DE-He213 Polyphasic taxonomy (dpeaa)DE-He213 Wetland sediment (dpeaa)DE-He213 Min, Ui-Gi verfasserin aut Kim, So-Jeong verfasserin aut Yim, Kyung June verfasserin aut Roh, Seong Woon verfasserin aut Rhee, Sung-Keun verfasserin aut Enthalten in Antonie van Leeuwenhoek Dordrecht [u.a.] : Springer Science + Business Media B.V, 1934 104(2013), 6 vom: 03. Sept., Seite 1005-1012 (DE-627)270929355 (DE-600)1478112-8 1572-9699 nnns volume:104 year:2013 number:6 day:03 month:09 pages:1005-1012 https://dx.doi.org/10.1007/s10482-013-0020-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE AR 104 2013 6 03 09 1005-1012 |
| allfieldsSound |
10.1007/s10482-013-0020-4 doi (DE-627)SPR010373624 (SPR)s10482-013-0020-4-e DE-627 ger DE-627 rakwb eng 570 610 ASE 42.30 bkl Cha, In-Tae verfasserin aut Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A novel mesophilic, methylotrophic, methanogenic archaeon, designated strain $ EK1^{T} $, was enriched and isolated from wetland sediment. Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. The type strain is strain $ EK1^{T} $(=NBRC $ 109636^{T} $ = KCTC $ 4119^{T} $ = JCM $ 19217^{T} $). Methanogen (dpeaa)DE-He213 Polyphasic taxonomy (dpeaa)DE-He213 Wetland sediment (dpeaa)DE-He213 Min, Ui-Gi verfasserin aut Kim, So-Jeong verfasserin aut Yim, Kyung June verfasserin aut Roh, Seong Woon verfasserin aut Rhee, Sung-Keun verfasserin aut Enthalten in Antonie van Leeuwenhoek Dordrecht [u.a.] : Springer Science + Business Media B.V, 1934 104(2013), 6 vom: 03. Sept., Seite 1005-1012 (DE-627)270929355 (DE-600)1478112-8 1572-9699 nnns volume:104 year:2013 number:6 day:03 month:09 pages:1005-1012 https://dx.doi.org/10.1007/s10482-013-0020-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE AR 104 2013 6 03 09 1005-1012 |
| language |
English |
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Enthalten in Antonie van Leeuwenhoek 104(2013), 6 vom: 03. Sept., Seite 1005-1012 volume:104 year:2013 number:6 day:03 month:09 pages:1005-1012 |
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Enthalten in Antonie van Leeuwenhoek 104(2013), 6 vom: 03. Sept., Seite 1005-1012 volume:104 year:2013 number:6 day:03 month:09 pages:1005-1012 |
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findex.gbv.de |
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Methanogen Polyphasic taxonomy Wetland sediment |
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570 |
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Antonie van Leeuwenhoek |
| authorswithroles_txt_mv |
Cha, In-Tae @@aut@@ Min, Ui-Gi @@aut@@ Kim, So-Jeong @@aut@@ Yim, Kyung June @@aut@@ Roh, Seong Woon @@aut@@ Rhee, Sung-Keun @@aut@@ |
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2013-09-03T00:00:00Z |
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englisch |
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Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. 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Cha, In-Tae |
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Cha, In-Tae ddc 570 bkl 42.30 misc Methanogen misc Polyphasic taxonomy misc Wetland sediment Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment |
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570 610 ASE 42.30 bkl Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment Methanogen (dpeaa)DE-He213 Polyphasic taxonomy (dpeaa)DE-He213 Wetland sediment (dpeaa)DE-He213 |
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ddc 570 bkl 42.30 misc Methanogen misc Polyphasic taxonomy misc Wetland sediment |
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ddc 570 bkl 42.30 misc Methanogen misc Polyphasic taxonomy misc Wetland sediment |
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Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment |
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Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment |
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Cha, In-Tae Min, Ui-Gi Kim, So-Jeong Yim, Kyung June Roh, Seong Woon Rhee, Sung-Keun |
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methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment |
| title_auth |
Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment |
| abstract |
Abstract A novel mesophilic, methylotrophic, methanogenic archaeon, designated strain $ EK1^{T} $, was enriched and isolated from wetland sediment. Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. The type strain is strain $ EK1^{T} $(=NBRC $ 109636^{T} $ = KCTC $ 4119^{T} $ = JCM $ 19217^{T} $). |
| abstractGer |
Abstract A novel mesophilic, methylotrophic, methanogenic archaeon, designated strain $ EK1^{T} $, was enriched and isolated from wetland sediment. Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. The type strain is strain $ EK1^{T} $(=NBRC $ 109636^{T} $ = KCTC $ 4119^{T} $ = JCM $ 19217^{T} $). |
| abstract_unstemmed |
Abstract A novel mesophilic, methylotrophic, methanogenic archaeon, designated strain $ EK1^{T} $, was enriched and isolated from wetland sediment. Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. The type strain is strain $ EK1^{T} $(=NBRC $ 109636^{T} $ = KCTC $ 4119^{T} $ = JCM $ 19217^{T} $). |
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Methanomethylovorans uponensis sp. nov., a methylotrophic methanogen isolated from wetland sediment |
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Min, Ui-Gi Kim, So-Jeong Yim, Kyung June Roh, Seong Woon Rhee, Sung-Keun |
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| doi_str |
10.1007/s10482-013-0020-4 |
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2024-07-03T15:42:53.047Z |
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Phylogenetic analysis showed that strain $ EK1^{T} $ was affiliated with the genus Methanomethylovorans within the family Methanosarcinaceae, and shared the highest 16S rRNA and methyl-coenzyme M reductase alpha-subunit gene sequence similarity with the type strain of Methanomethylovorans hollandica (98.8 and 92.6 %, respectively). The cells of strain $ EK1^{T} $ were observed to be Gram-negative, non-motile and irregular cocci that did not lyse in 0.1 % (w/v) sodium dodecyl sulfate. Methanol, mono-, di- and trimethylamine, dimethyl sulfide and methanethiol were found to be used as catabolic and methanogenic substrates, whereas $ H_{2} $/$ CO_{2} $, formate, 2-propanol and acetate were not. Growth was observed at 25–40 °C (optimum, 37 °C), at pH 5.5–7.5 (optimum, pH 6.0–6.5) and in the presence of 0–0.1 M NaCl (optimum, 0 M). Growth and methane production rates were stimulated in the presence of $ H_{2} $/$ CO_{2} $ although methane production and growth yields were not significantly affected; acetate, formate, 2-propanol and CO/$ CO_{2} $/$ N_{2} $ did not affect methane production. $ CoCl_{2} $ (0.6–2.0 μM) and $ FeCl_{2} $ (25 mg/l) stimulated growth, while yeast extract and peptone did not. The DNA–DNA hybridization experiment revealed a relatedness of <20 % between $ EK1^{T} $ and the type strains of the genus Methanomethylovorans. The DNA G+C content of strain $ EK1^{T} $ was determined to be 39.2 mol%. Based on the polyphasic taxonomic study, strain $ EK1^{T} $ represents a novel species belonging to the genus Methanomethylovorans, for which the name Methanomethylovorans uponensis sp. nov. is proposed. 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