Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis
Abstract Six promoters in the 419 bp upstream sequence of the phycocyanin β subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of pro...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Guo, Nan [verfasserIn] Zhang, Xuecheng [verfasserIn] Lu, Yongzhong [verfasserIn] Song, Xiaojin [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2007 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Biotechnology letters - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979, 29(2007), 3 vom: 23. Jan., Seite 459-464 |
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| Übergeordnetes Werk: |
volume:29 ; year:2007 ; number:3 ; day:23 ; month:01 ; pages:459-464 |
| Links: |
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| DOI / URN: |
10.1007/s10529-006-9266-5 |
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| Katalog-ID: |
SPR010837337 |
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| 100 | 1 | |a Guo, Nan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis |
| 264 | 1 | |c 2007 | |
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| 520 | |a Abstract Six promoters in the 419 bp upstream sequence of the phycocyanin β subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined. | ||
| 650 | 4 | |a Gene |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Green fluorescent protein |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Site-directed mutagenesis |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Zhang, Xuecheng |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Yongzhong |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Xiaojin |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biotechnology letters |d Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 |g 29(2007), 3 vom: 23. Jan., Seite 459-464 |w (DE-627)312808356 |w (DE-600)2012203-2 |x 1573-6776 |7 nnns |
| 773 | 1 | 8 | |g volume:29 |g year:2007 |g number:3 |g day:23 |g month:01 |g pages:459-464 |
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| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
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| 912 | |a GBV_ILN_90 | ||
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| 912 | |a GBV_ILN_105 | ||
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| 912 | |a GBV_ILN_281 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
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| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2007 | ||
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| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
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| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
| 912 | |a GBV_ILN_4700 | ||
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| allfields |
10.1007/s10529-006-9266-5 doi (DE-627)SPR010837337 (SPR)s10529-006-9266-5-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Guo, Nan verfasserin aut Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Six promoters in the 419 bp upstream sequence of the phycocyanin β subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined. Gene (dpeaa)DE-He213 Green fluorescent protein (dpeaa)DE-He213 Site-directed mutagenesis (dpeaa)DE-He213 Zhang, Xuecheng verfasserin aut Lu, Yongzhong verfasserin aut Song, Xiaojin verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 29(2007), 3 vom: 23. Jan., Seite 459-464 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:29 year:2007 number:3 day:23 month:01 pages:459-464 https://dx.doi.org/10.1007/s10529-006-9266-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 29 2007 3 23 01 459-464 |
| spelling |
10.1007/s10529-006-9266-5 doi (DE-627)SPR010837337 (SPR)s10529-006-9266-5-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Guo, Nan verfasserin aut Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Six promoters in the 419 bp upstream sequence of the phycocyanin β subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined. Gene (dpeaa)DE-He213 Green fluorescent protein (dpeaa)DE-He213 Site-directed mutagenesis (dpeaa)DE-He213 Zhang, Xuecheng verfasserin aut Lu, Yongzhong verfasserin aut Song, Xiaojin verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 29(2007), 3 vom: 23. Jan., Seite 459-464 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:29 year:2007 number:3 day:23 month:01 pages:459-464 https://dx.doi.org/10.1007/s10529-006-9266-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 29 2007 3 23 01 459-464 |
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10.1007/s10529-006-9266-5 doi (DE-627)SPR010837337 (SPR)s10529-006-9266-5-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Guo, Nan verfasserin aut Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Six promoters in the 419 bp upstream sequence of the phycocyanin β subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined. Gene (dpeaa)DE-He213 Green fluorescent protein (dpeaa)DE-He213 Site-directed mutagenesis (dpeaa)DE-He213 Zhang, Xuecheng verfasserin aut Lu, Yongzhong verfasserin aut Song, Xiaojin verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 29(2007), 3 vom: 23. Jan., Seite 459-464 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:29 year:2007 number:3 day:23 month:01 pages:459-464 https://dx.doi.org/10.1007/s10529-006-9266-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 29 2007 3 23 01 459-464 |
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10.1007/s10529-006-9266-5 doi (DE-627)SPR010837337 (SPR)s10529-006-9266-5-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Guo, Nan verfasserin aut Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Six promoters in the 419 bp upstream sequence of the phycocyanin β subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined. Gene (dpeaa)DE-He213 Green fluorescent protein (dpeaa)DE-He213 Site-directed mutagenesis (dpeaa)DE-He213 Zhang, Xuecheng verfasserin aut Lu, Yongzhong verfasserin aut Song, Xiaojin verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 29(2007), 3 vom: 23. Jan., Seite 459-464 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:29 year:2007 number:3 day:23 month:01 pages:459-464 https://dx.doi.org/10.1007/s10529-006-9266-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 29 2007 3 23 01 459-464 |
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English |
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Enthalten in Biotechnology letters 29(2007), 3 vom: 23. Jan., Seite 459-464 volume:29 year:2007 number:3 day:23 month:01 pages:459-464 |
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Enthalten in Biotechnology letters 29(2007), 3 vom: 23. Jan., Seite 459-464 volume:29 year:2007 number:3 day:23 month:01 pages:459-464 |
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Gene Green fluorescent protein Site-directed mutagenesis |
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Biotechnology letters |
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Guo, Nan @@aut@@ Zhang, Xuecheng @@aut@@ Lu, Yongzhong @@aut@@ Song, Xiaojin @@aut@@ |
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2007-01-23T00:00:00Z |
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312808356 |
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3660 |
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| author |
Guo, Nan |
| spellingShingle |
Guo, Nan ddc 660 bkl 42.30 bkl 58.30 misc Gene misc Green fluorescent protein misc Site-directed mutagenesis Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis |
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660 ASE 42.30 bkl 58.30 bkl Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis Gene (dpeaa)DE-He213 Green fluorescent protein (dpeaa)DE-He213 Site-directed mutagenesis (dpeaa)DE-He213 |
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ddc 660 bkl 42.30 bkl 58.30 misc Gene misc Green fluorescent protein misc Site-directed mutagenesis |
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ddc 660 bkl 42.30 bkl 58.30 misc Gene misc Green fluorescent protein misc Site-directed mutagenesis |
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Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis |
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(DE-627)SPR010837337 (SPR)s10529-006-9266-5-e |
| title_full |
Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis |
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Guo, Nan |
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Guo, Nan Zhang, Xuecheng Lu, Yongzhong Song, Xiaojin |
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verfasserin |
| title_sort |
analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from arthrospira platensis by site-directed mutagenesis |
| title_auth |
Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis |
| abstract |
Abstract Six promoters in the 419 bp upstream sequence of the phycocyanin β subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined. |
| abstractGer |
Abstract Six promoters in the 419 bp upstream sequence of the phycocyanin β subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined. |
| abstract_unstemmed |
Abstract Six promoters in the 419 bp upstream sequence of the phycocyanin β subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined. |
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| container_issue |
3 |
| title_short |
Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis |
| url |
https://dx.doi.org/10.1007/s10529-006-9266-5 |
| remote_bool |
true |
| author2 |
Zhang, Xuecheng Lu, Yongzhong Song, Xiaojin |
| author2Str |
Zhang, Xuecheng Lu, Yongzhong Song, Xiaojin |
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312808356 |
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c |
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false |
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false |
| doi_str |
10.1007/s10529-006-9266-5 |
| up_date |
2024-07-03T18:37:28.799Z |
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1803584115903037441 |
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| score |
7.3998337 |