Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium
Abstract Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lee, Yong Kwon [verfasserIn] Kim, Seung Yeul [verfasserIn] Kim, Ki Heon [verfasserIn] Chun, Bok-Hwan [verfasserIn] Lee, Kweon-Haeng [verfasserIn] Oh, Duk Jae [verfasserIn] Chung, Namhyun [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2008 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Biotechnology letters - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979, 30(2008), 11 vom: 09. Juli, Seite 1931-1936 |
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| Übergeordnetes Werk: |
volume:30 ; year:2008 ; number:11 ; day:09 ; month:07 ; pages:1931-1936 |
| Links: |
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| DOI / URN: |
10.1007/s10529-008-9796-0 |
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| Katalog-ID: |
SPR010839577 |
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| 245 | 1 | 0 | |a Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium |
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| 520 | |a Abstract Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE. | ||
| 650 | 4 | |a Soybean protein hydrolysates |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Cell growth |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Keratinocytes |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Serum-free medium |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Kim, Seung Yeul |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Ki Heon |e verfasserin |4 aut | |
| 700 | 1 | |a Chun, Bok-Hwan |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Kweon-Haeng |e verfasserin |4 aut | |
| 700 | 1 | |a Oh, Duk Jae |e verfasserin |4 aut | |
| 700 | 1 | |a Chung, Namhyun |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biotechnology letters |d Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 |g 30(2008), 11 vom: 09. Juli, Seite 1931-1936 |w (DE-627)312808356 |w (DE-600)2012203-2 |x 1573-6776 |7 nnns |
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| 912 | |a GBV_ILN_2064 | ||
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| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
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| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
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10.1007/s10529-008-9796-0 doi (DE-627)SPR010839577 (SPR)s10529-008-9796-0-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Lee, Yong Kwon verfasserin aut Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE. Soybean protein hydrolysates (dpeaa)DE-He213 Cell growth (dpeaa)DE-He213 Keratinocytes (dpeaa)DE-He213 Serum-free medium (dpeaa)DE-He213 Kim, Seung Yeul verfasserin aut Kim, Ki Heon verfasserin aut Chun, Bok-Hwan verfasserin aut Lee, Kweon-Haeng verfasserin aut Oh, Duk Jae verfasserin aut Chung, Namhyun verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 30(2008), 11 vom: 09. Juli, Seite 1931-1936 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:30 year:2008 number:11 day:09 month:07 pages:1931-1936 https://dx.doi.org/10.1007/s10529-008-9796-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 30 2008 11 09 07 1931-1936 |
| spelling |
10.1007/s10529-008-9796-0 doi (DE-627)SPR010839577 (SPR)s10529-008-9796-0-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Lee, Yong Kwon verfasserin aut Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE. Soybean protein hydrolysates (dpeaa)DE-He213 Cell growth (dpeaa)DE-He213 Keratinocytes (dpeaa)DE-He213 Serum-free medium (dpeaa)DE-He213 Kim, Seung Yeul verfasserin aut Kim, Ki Heon verfasserin aut Chun, Bok-Hwan verfasserin aut Lee, Kweon-Haeng verfasserin aut Oh, Duk Jae verfasserin aut Chung, Namhyun verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 30(2008), 11 vom: 09. Juli, Seite 1931-1936 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:30 year:2008 number:11 day:09 month:07 pages:1931-1936 https://dx.doi.org/10.1007/s10529-008-9796-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 30 2008 11 09 07 1931-1936 |
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10.1007/s10529-008-9796-0 doi (DE-627)SPR010839577 (SPR)s10529-008-9796-0-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Lee, Yong Kwon verfasserin aut Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE. Soybean protein hydrolysates (dpeaa)DE-He213 Cell growth (dpeaa)DE-He213 Keratinocytes (dpeaa)DE-He213 Serum-free medium (dpeaa)DE-He213 Kim, Seung Yeul verfasserin aut Kim, Ki Heon verfasserin aut Chun, Bok-Hwan verfasserin aut Lee, Kweon-Haeng verfasserin aut Oh, Duk Jae verfasserin aut Chung, Namhyun verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 30(2008), 11 vom: 09. Juli, Seite 1931-1936 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:30 year:2008 number:11 day:09 month:07 pages:1931-1936 https://dx.doi.org/10.1007/s10529-008-9796-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 30 2008 11 09 07 1931-1936 |
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10.1007/s10529-008-9796-0 doi (DE-627)SPR010839577 (SPR)s10529-008-9796-0-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Lee, Yong Kwon verfasserin aut Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE. Soybean protein hydrolysates (dpeaa)DE-He213 Cell growth (dpeaa)DE-He213 Keratinocytes (dpeaa)DE-He213 Serum-free medium (dpeaa)DE-He213 Kim, Seung Yeul verfasserin aut Kim, Ki Heon verfasserin aut Chun, Bok-Hwan verfasserin aut Lee, Kweon-Haeng verfasserin aut Oh, Duk Jae verfasserin aut Chung, Namhyun verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 30(2008), 11 vom: 09. Juli, Seite 1931-1936 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:30 year:2008 number:11 day:09 month:07 pages:1931-1936 https://dx.doi.org/10.1007/s10529-008-9796-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 30 2008 11 09 07 1931-1936 |
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10.1007/s10529-008-9796-0 doi (DE-627)SPR010839577 (SPR)s10529-008-9796-0-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Lee, Yong Kwon verfasserin aut Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE. Soybean protein hydrolysates (dpeaa)DE-He213 Cell growth (dpeaa)DE-He213 Keratinocytes (dpeaa)DE-He213 Serum-free medium (dpeaa)DE-He213 Kim, Seung Yeul verfasserin aut Kim, Ki Heon verfasserin aut Chun, Bok-Hwan verfasserin aut Lee, Kweon-Haeng verfasserin aut Oh, Duk Jae verfasserin aut Chung, Namhyun verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 30(2008), 11 vom: 09. Juli, Seite 1931-1936 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:30 year:2008 number:11 day:09 month:07 pages:1931-1936 https://dx.doi.org/10.1007/s10529-008-9796-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 30 2008 11 09 07 1931-1936 |
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Enthalten in Biotechnology letters 30(2008), 11 vom: 09. Juli, Seite 1931-1936 volume:30 year:2008 number:11 day:09 month:07 pages:1931-1936 |
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Enthalten in Biotechnology letters 30(2008), 11 vom: 09. Juli, Seite 1931-1936 volume:30 year:2008 number:11 day:09 month:07 pages:1931-1936 |
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Soybean protein hydrolysates Cell growth Keratinocytes Serum-free medium |
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Biotechnology letters |
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Lee, Yong Kwon @@aut@@ Kim, Seung Yeul @@aut@@ Kim, Ki Heon @@aut@@ Chun, Bok-Hwan @@aut@@ Lee, Kweon-Haeng @@aut@@ Oh, Duk Jae @@aut@@ Chung, Namhyun @@aut@@ |
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2008-07-09T00:00:00Z |
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Lee, Yong Kwon |
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Lee, Yong Kwon ddc 660 bkl 42.30 bkl 58.30 misc Soybean protein hydrolysates misc Cell growth misc Keratinocytes misc Serum-free medium Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium |
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660 ASE 42.30 bkl 58.30 bkl Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium Soybean protein hydrolysates (dpeaa)DE-He213 Cell growth (dpeaa)DE-He213 Keratinocytes (dpeaa)DE-He213 Serum-free medium (dpeaa)DE-He213 |
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ddc 660 bkl 42.30 bkl 58.30 misc Soybean protein hydrolysates misc Cell growth misc Keratinocytes misc Serum-free medium |
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ddc 660 bkl 42.30 bkl 58.30 misc Soybean protein hydrolysates misc Cell growth misc Keratinocytes misc Serum-free medium |
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Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium |
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Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium |
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Lee, Yong Kwon Kim, Seung Yeul Kim, Ki Heon Chun, Bok-Hwan Lee, Kweon-Haeng Oh, Duk Jae Chung, Namhyun |
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use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium |
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Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium |
| abstract |
Abstract Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE. |
| abstractGer |
Abstract Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE. |
| abstract_unstemmed |
Abstract Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE. |
| collection_details |
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| container_issue |
11 |
| title_short |
Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium |
| url |
https://dx.doi.org/10.1007/s10529-008-9796-0 |
| remote_bool |
true |
| author2 |
Kim, Seung Yeul Kim, Ki Heon Chun, Bok-Hwan Lee, Kweon-Haeng Oh, Duk Jae Chung, Namhyun |
| author2Str |
Kim, Seung Yeul Kim, Ki Heon Chun, Bok-Hwan Lee, Kweon-Haeng Oh, Duk Jae Chung, Namhyun |
| ppnlink |
312808356 |
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c |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1007/s10529-008-9796-0 |
| up_date |
2024-07-03T18:38:21.856Z |
| _version_ |
1803584171523702784 |
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| score |
7.4031916 |