Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells
Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $,...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Fan, Li [verfasserIn] Zhao, Liang [verfasserIn] Ye, Zhaoyang [verfasserIn] Sun, Yating [verfasserIn] Kou, Tianci [verfasserIn] Zhou, Yan [verfasserIn] Tan, Wen-Song [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Biotechnology letters - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979, 32(2010), 9 vom: 09. Juni, Seite 1239-1244 |
|---|---|
| Übergeordnetes Werk: |
volume:32 ; year:2010 ; number:9 ; day:09 ; month:06 ; pages:1239-1244 |
| Links: |
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| DOI / URN: |
10.1007/s10529-010-0318-5 |
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| Katalog-ID: |
SPR010847669 |
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| 245 | 1 | 0 | |a Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells |
| 264 | 1 | |c 2010 | |
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| 520 | |a Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $, increased as culture temperature decreased; and the maximum $ q_{TNFR-Fc} $ was 20 mg/($ 10^{9} $ cells·day) at 30°C which was three times that at 37°C. Increasing the viable cell density (VCD) to above 2.2 × $ 10^{6} $ cells/ml, however, decreased the $ q_{TNFR-Fc} $ at 30°C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve $ q_{TNFR-Fc} $ but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed. | ||
| 650 | 4 | |a CHO cells |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Culture temperature |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Specific productivity |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a TNFR-Fc |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Zhao, Liang |e verfasserin |4 aut | |
| 700 | 1 | |a Ye, Zhaoyang |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Yating |e verfasserin |4 aut | |
| 700 | 1 | |a Kou, Tianci |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Wen-Song |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biotechnology letters |d Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 |g 32(2010), 9 vom: 09. Juni, Seite 1239-1244 |w (DE-627)312808356 |w (DE-600)2012203-2 |x 1573-6776 |7 nnns |
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| allfields |
10.1007/s10529-010-0318-5 doi (DE-627)SPR010847669 (SPR)s10529-010-0318-5-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Fan, Li verfasserin aut Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $, increased as culture temperature decreased; and the maximum $ q_{TNFR-Fc} $ was 20 mg/($ 10^{9} $ cells·day) at 30°C which was three times that at 37°C. Increasing the viable cell density (VCD) to above 2.2 × $ 10^{6} $ cells/ml, however, decreased the $ q_{TNFR-Fc} $ at 30°C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve $ q_{TNFR-Fc} $ but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed. CHO cells (dpeaa)DE-He213 Culture temperature (dpeaa)DE-He213 Specific productivity (dpeaa)DE-He213 TNFR-Fc (dpeaa)DE-He213 Zhao, Liang verfasserin aut Ye, Zhaoyang verfasserin aut Sun, Yating verfasserin aut Kou, Tianci verfasserin aut Zhou, Yan verfasserin aut Tan, Wen-Song verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 32(2010), 9 vom: 09. Juni, Seite 1239-1244 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:32 year:2010 number:9 day:09 month:06 pages:1239-1244 https://dx.doi.org/10.1007/s10529-010-0318-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 32 2010 9 09 06 1239-1244 |
| spelling |
10.1007/s10529-010-0318-5 doi (DE-627)SPR010847669 (SPR)s10529-010-0318-5-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Fan, Li verfasserin aut Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $, increased as culture temperature decreased; and the maximum $ q_{TNFR-Fc} $ was 20 mg/($ 10^{9} $ cells·day) at 30°C which was three times that at 37°C. Increasing the viable cell density (VCD) to above 2.2 × $ 10^{6} $ cells/ml, however, decreased the $ q_{TNFR-Fc} $ at 30°C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve $ q_{TNFR-Fc} $ but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed. CHO cells (dpeaa)DE-He213 Culture temperature (dpeaa)DE-He213 Specific productivity (dpeaa)DE-He213 TNFR-Fc (dpeaa)DE-He213 Zhao, Liang verfasserin aut Ye, Zhaoyang verfasserin aut Sun, Yating verfasserin aut Kou, Tianci verfasserin aut Zhou, Yan verfasserin aut Tan, Wen-Song verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 32(2010), 9 vom: 09. Juni, Seite 1239-1244 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:32 year:2010 number:9 day:09 month:06 pages:1239-1244 https://dx.doi.org/10.1007/s10529-010-0318-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 32 2010 9 09 06 1239-1244 |
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10.1007/s10529-010-0318-5 doi (DE-627)SPR010847669 (SPR)s10529-010-0318-5-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Fan, Li verfasserin aut Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $, increased as culture temperature decreased; and the maximum $ q_{TNFR-Fc} $ was 20 mg/($ 10^{9} $ cells·day) at 30°C which was three times that at 37°C. Increasing the viable cell density (VCD) to above 2.2 × $ 10^{6} $ cells/ml, however, decreased the $ q_{TNFR-Fc} $ at 30°C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve $ q_{TNFR-Fc} $ but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed. CHO cells (dpeaa)DE-He213 Culture temperature (dpeaa)DE-He213 Specific productivity (dpeaa)DE-He213 TNFR-Fc (dpeaa)DE-He213 Zhao, Liang verfasserin aut Ye, Zhaoyang verfasserin aut Sun, Yating verfasserin aut Kou, Tianci verfasserin aut Zhou, Yan verfasserin aut Tan, Wen-Song verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 32(2010), 9 vom: 09. Juni, Seite 1239-1244 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:32 year:2010 number:9 day:09 month:06 pages:1239-1244 https://dx.doi.org/10.1007/s10529-010-0318-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 32 2010 9 09 06 1239-1244 |
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10.1007/s10529-010-0318-5 doi (DE-627)SPR010847669 (SPR)s10529-010-0318-5-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Fan, Li verfasserin aut Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $, increased as culture temperature decreased; and the maximum $ q_{TNFR-Fc} $ was 20 mg/($ 10^{9} $ cells·day) at 30°C which was three times that at 37°C. Increasing the viable cell density (VCD) to above 2.2 × $ 10^{6} $ cells/ml, however, decreased the $ q_{TNFR-Fc} $ at 30°C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve $ q_{TNFR-Fc} $ but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed. CHO cells (dpeaa)DE-He213 Culture temperature (dpeaa)DE-He213 Specific productivity (dpeaa)DE-He213 TNFR-Fc (dpeaa)DE-He213 Zhao, Liang verfasserin aut Ye, Zhaoyang verfasserin aut Sun, Yating verfasserin aut Kou, Tianci verfasserin aut Zhou, Yan verfasserin aut Tan, Wen-Song verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 32(2010), 9 vom: 09. Juni, Seite 1239-1244 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:32 year:2010 number:9 day:09 month:06 pages:1239-1244 https://dx.doi.org/10.1007/s10529-010-0318-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 32 2010 9 09 06 1239-1244 |
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10.1007/s10529-010-0318-5 doi (DE-627)SPR010847669 (SPR)s10529-010-0318-5-e DE-627 ger DE-627 rakwb eng 660 ASE 42.30 bkl 58.30 bkl Fan, Li verfasserin aut Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $, increased as culture temperature decreased; and the maximum $ q_{TNFR-Fc} $ was 20 mg/($ 10^{9} $ cells·day) at 30°C which was three times that at 37°C. Increasing the viable cell density (VCD) to above 2.2 × $ 10^{6} $ cells/ml, however, decreased the $ q_{TNFR-Fc} $ at 30°C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve $ q_{TNFR-Fc} $ but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed. CHO cells (dpeaa)DE-He213 Culture temperature (dpeaa)DE-He213 Specific productivity (dpeaa)DE-He213 TNFR-Fc (dpeaa)DE-He213 Zhao, Liang verfasserin aut Ye, Zhaoyang verfasserin aut Sun, Yating verfasserin aut Kou, Tianci verfasserin aut Zhou, Yan verfasserin aut Tan, Wen-Song verfasserin aut Enthalten in Biotechnology letters Dordrecht [u.a.] : Springer Science + Business Media B.V, 1979 32(2010), 9 vom: 09. Juni, Seite 1239-1244 (DE-627)312808356 (DE-600)2012203-2 1573-6776 nnns volume:32 year:2010 number:9 day:09 month:06 pages:1239-1244 https://dx.doi.org/10.1007/s10529-010-0318-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.30 ASE 58.30 ASE AR 32 2010 9 09 06 1239-1244 |
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Enthalten in Biotechnology letters 32(2010), 9 vom: 09. Juni, Seite 1239-1244 volume:32 year:2010 number:9 day:09 month:06 pages:1239-1244 |
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CHO cells Culture temperature Specific productivity TNFR-Fc |
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Fan, Li @@aut@@ Zhao, Liang @@aut@@ Ye, Zhaoyang @@aut@@ Sun, Yating @@aut@@ Kou, Tianci @@aut@@ Zhou, Yan @@aut@@ Tan, Wen-Song @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR010847669</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519185739.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201005s2010 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s10529-010-0318-5</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR010847669</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s10529-010-0318-5-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">660</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.30</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">58.30</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Fan, Li</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2010</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $, increased as culture temperature decreased; and the maximum $ q_{TNFR-Fc} $ was 20 mg/($ 10^{9} $ cells·day) at 30°C which was three times that at 37°C. Increasing the viable cell density (VCD) to above 2.2 × $ 10^{6} $ cells/ml, however, decreased the $ q_{TNFR-Fc} $ at 30°C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve $ q_{TNFR-Fc} $ but the negative effect of increasing VCD compromises this effect. 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Fan, Li |
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Fan, Li ddc 660 bkl 42.30 bkl 58.30 misc CHO cells misc Culture temperature misc Specific productivity misc TNFR-Fc Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells |
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660 ASE 42.30 bkl 58.30 bkl Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells CHO cells (dpeaa)DE-He213 Culture temperature (dpeaa)DE-He213 Specific productivity (dpeaa)DE-He213 TNFR-Fc (dpeaa)DE-He213 |
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Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells |
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Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells |
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effect of culture temperature on tnfr-fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells |
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Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells |
| abstract |
Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $, increased as culture temperature decreased; and the maximum $ q_{TNFR-Fc} $ was 20 mg/($ 10^{9} $ cells·day) at 30°C which was three times that at 37°C. Increasing the viable cell density (VCD) to above 2.2 × $ 10^{6} $ cells/ml, however, decreased the $ q_{TNFR-Fc} $ at 30°C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve $ q_{TNFR-Fc} $ but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed. |
| abstractGer |
Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $, increased as culture temperature decreased; and the maximum $ q_{TNFR-Fc} $ was 20 mg/($ 10^{9} $ cells·day) at 30°C which was three times that at 37°C. Increasing the viable cell density (VCD) to above 2.2 × $ 10^{6} $ cells/ml, however, decreased the $ q_{TNFR-Fc} $ at 30°C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve $ q_{TNFR-Fc} $ but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed. |
| abstract_unstemmed |
Abstract Lowering the culture temperature is often useful to improve the production of many recombinant proteins in Chinese hamster ovary (CHO) cells. Batch cultivation of GS-CHO cells expressing TNFR-Fc antibody was therefore carried out at 30, 33.5 and 37°C. TNFR-Fc productivity, $ q_{TNFR-Fc} $, increased as culture temperature decreased; and the maximum $ q_{TNFR-Fc} $ was 20 mg/($ 10^{9} $ cells·day) at 30°C which was three times that at 37°C. Increasing the viable cell density (VCD) to above 2.2 × $ 10^{6} $ cells/ml, however, decreased the $ q_{TNFR-Fc} $ at 30°C, which was due to a reduction in transcription of the TNFR-Fc gene. Taken together, lowering temperature can improve $ q_{TNFR-Fc} $ but the negative effect of increasing VCD compromises this effect. Further process development addressing the issue of cell density-dependent TNFR-Fc productivity is therefore needed. |
| collection_details |
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| container_issue |
9 |
| title_short |
Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells |
| url |
https://dx.doi.org/10.1007/s10529-010-0318-5 |
| remote_bool |
true |
| author2 |
Zhao, Liang Ye, Zhaoyang Sun, Yating Kou, Tianci Zhou, Yan Tan, Wen-Song |
| author2Str |
Zhao, Liang Ye, Zhaoyang Sun, Yating Kou, Tianci Zhou, Yan Tan, Wen-Song |
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312808356 |
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false |
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| doi_str |
10.1007/s10529-010-0318-5 |
| up_date |
2024-07-03T18:41:32.211Z |
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| score |
7.3997555 |