Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules
Abstract Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We h...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Sugiura, Shinji [verfasserIn] Oda, Tatsuya [verfasserIn] Aoyagi, Yasuyuki [verfasserIn] Matsuo, Ryota [verfasserIn] Enomoto, Tsuyoshi [verfasserIn] Matsumoto, Kunio [verfasserIn] Nakamura, Toshikazu [verfasserIn] Satake, Mitsuo [verfasserIn] Ochiai, Atsushi [verfasserIn] Ohkohchi, Nobuhiro [verfasserIn] Nakajima, Mitsutoshi [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2006 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Biomedical microdevices - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998, 9(2006), 1 vom: 11. Nov., Seite 91-99 |
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| Übergeordnetes Werk: |
volume:9 ; year:2006 ; number:1 ; day:11 ; month:11 ; pages:91-99 |
| Links: |
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| DOI / URN: |
10.1007/s10544-006-9011-9 |
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| Katalog-ID: |
SPR01116803X |
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| 100 | 1 | |a Sugiura, Shinji |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules |
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| 520 | |a Abstract Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a $ CaCl_{2} $ solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. The advantages of smaller microcapsules offer new prospects for the advancement of microencapsulation technology. | ||
| 650 | 4 | |a Material fabrication |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Cell encapsulation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Microcapsule |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Size control |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Microfluidic device |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Oda, Tatsuya |e verfasserin |4 aut | |
| 700 | 1 | |a Aoyagi, Yasuyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Matsuo, Ryota |e verfasserin |4 aut | |
| 700 | 1 | |a Enomoto, Tsuyoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Matsumoto, Kunio |e verfasserin |4 aut | |
| 700 | 1 | |a Nakamura, Toshikazu |e verfasserin |4 aut | |
| 700 | 1 | |a Satake, Mitsuo |e verfasserin |4 aut | |
| 700 | 1 | |a Ochiai, Atsushi |e verfasserin |4 aut | |
| 700 | 1 | |a Ohkohchi, Nobuhiro |e verfasserin |4 aut | |
| 700 | 1 | |a Nakajima, Mitsutoshi |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biomedical microdevices |d Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 |g 9(2006), 1 vom: 11. Nov., Seite 91-99 |w (DE-627)320433269 |w (DE-600)2004019-2 |x 1572-8781 |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2006 |g number:1 |g day:11 |g month:11 |g pages:91-99 |
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| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
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| 912 | |a GBV_ILN_2110 | ||
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| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
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| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
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| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
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| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
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| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
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10.1007/s10544-006-9011-9 doi (DE-627)SPR01116803X (SPR)s10544-006-9011-9-e DE-627 ger DE-627 rakwb eng 570 ASE 44.00 bkl Sugiura, Shinji verfasserin aut Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a $ CaCl_{2} $ solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. The advantages of smaller microcapsules offer new prospects for the advancement of microencapsulation technology. Material fabrication (dpeaa)DE-He213 Cell encapsulation (dpeaa)DE-He213 Microcapsule (dpeaa)DE-He213 Size control (dpeaa)DE-He213 Microfluidic device (dpeaa)DE-He213 Oda, Tatsuya verfasserin aut Aoyagi, Yasuyuki verfasserin aut Matsuo, Ryota verfasserin aut Enomoto, Tsuyoshi verfasserin aut Matsumoto, Kunio verfasserin aut Nakamura, Toshikazu verfasserin aut Satake, Mitsuo verfasserin aut Ochiai, Atsushi verfasserin aut Ohkohchi, Nobuhiro verfasserin aut Nakajima, Mitsutoshi verfasserin aut Enthalten in Biomedical microdevices Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 9(2006), 1 vom: 11. Nov., Seite 91-99 (DE-627)320433269 (DE-600)2004019-2 1572-8781 nnns volume:9 year:2006 number:1 day:11 month:11 pages:91-99 https://dx.doi.org/10.1007/s10544-006-9011-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE AR 9 2006 1 11 11 91-99 |
| spelling |
10.1007/s10544-006-9011-9 doi (DE-627)SPR01116803X (SPR)s10544-006-9011-9-e DE-627 ger DE-627 rakwb eng 570 ASE 44.00 bkl Sugiura, Shinji verfasserin aut Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a $ CaCl_{2} $ solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. The advantages of smaller microcapsules offer new prospects for the advancement of microencapsulation technology. Material fabrication (dpeaa)DE-He213 Cell encapsulation (dpeaa)DE-He213 Microcapsule (dpeaa)DE-He213 Size control (dpeaa)DE-He213 Microfluidic device (dpeaa)DE-He213 Oda, Tatsuya verfasserin aut Aoyagi, Yasuyuki verfasserin aut Matsuo, Ryota verfasserin aut Enomoto, Tsuyoshi verfasserin aut Matsumoto, Kunio verfasserin aut Nakamura, Toshikazu verfasserin aut Satake, Mitsuo verfasserin aut Ochiai, Atsushi verfasserin aut Ohkohchi, Nobuhiro verfasserin aut Nakajima, Mitsutoshi verfasserin aut Enthalten in Biomedical microdevices Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 9(2006), 1 vom: 11. Nov., Seite 91-99 (DE-627)320433269 (DE-600)2004019-2 1572-8781 nnns volume:9 year:2006 number:1 day:11 month:11 pages:91-99 https://dx.doi.org/10.1007/s10544-006-9011-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE AR 9 2006 1 11 11 91-99 |
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10.1007/s10544-006-9011-9 doi (DE-627)SPR01116803X (SPR)s10544-006-9011-9-e DE-627 ger DE-627 rakwb eng 570 ASE 44.00 bkl Sugiura, Shinji verfasserin aut Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a $ CaCl_{2} $ solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. The advantages of smaller microcapsules offer new prospects for the advancement of microencapsulation technology. Material fabrication (dpeaa)DE-He213 Cell encapsulation (dpeaa)DE-He213 Microcapsule (dpeaa)DE-He213 Size control (dpeaa)DE-He213 Microfluidic device (dpeaa)DE-He213 Oda, Tatsuya verfasserin aut Aoyagi, Yasuyuki verfasserin aut Matsuo, Ryota verfasserin aut Enomoto, Tsuyoshi verfasserin aut Matsumoto, Kunio verfasserin aut Nakamura, Toshikazu verfasserin aut Satake, Mitsuo verfasserin aut Ochiai, Atsushi verfasserin aut Ohkohchi, Nobuhiro verfasserin aut Nakajima, Mitsutoshi verfasserin aut Enthalten in Biomedical microdevices Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 9(2006), 1 vom: 11. Nov., Seite 91-99 (DE-627)320433269 (DE-600)2004019-2 1572-8781 nnns volume:9 year:2006 number:1 day:11 month:11 pages:91-99 https://dx.doi.org/10.1007/s10544-006-9011-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE AR 9 2006 1 11 11 91-99 |
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10.1007/s10544-006-9011-9 doi (DE-627)SPR01116803X (SPR)s10544-006-9011-9-e DE-627 ger DE-627 rakwb eng 570 ASE 44.00 bkl Sugiura, Shinji verfasserin aut Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a $ CaCl_{2} $ solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. The advantages of smaller microcapsules offer new prospects for the advancement of microencapsulation technology. Material fabrication (dpeaa)DE-He213 Cell encapsulation (dpeaa)DE-He213 Microcapsule (dpeaa)DE-He213 Size control (dpeaa)DE-He213 Microfluidic device (dpeaa)DE-He213 Oda, Tatsuya verfasserin aut Aoyagi, Yasuyuki verfasserin aut Matsuo, Ryota verfasserin aut Enomoto, Tsuyoshi verfasserin aut Matsumoto, Kunio verfasserin aut Nakamura, Toshikazu verfasserin aut Satake, Mitsuo verfasserin aut Ochiai, Atsushi verfasserin aut Ohkohchi, Nobuhiro verfasserin aut Nakajima, Mitsutoshi verfasserin aut Enthalten in Biomedical microdevices Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 9(2006), 1 vom: 11. Nov., Seite 91-99 (DE-627)320433269 (DE-600)2004019-2 1572-8781 nnns volume:9 year:2006 number:1 day:11 month:11 pages:91-99 https://dx.doi.org/10.1007/s10544-006-9011-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE AR 9 2006 1 11 11 91-99 |
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10.1007/s10544-006-9011-9 doi (DE-627)SPR01116803X (SPR)s10544-006-9011-9-e DE-627 ger DE-627 rakwb eng 570 ASE 44.00 bkl Sugiura, Shinji verfasserin aut Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a $ CaCl_{2} $ solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. The advantages of smaller microcapsules offer new prospects for the advancement of microencapsulation technology. Material fabrication (dpeaa)DE-He213 Cell encapsulation (dpeaa)DE-He213 Microcapsule (dpeaa)DE-He213 Size control (dpeaa)DE-He213 Microfluidic device (dpeaa)DE-He213 Oda, Tatsuya verfasserin aut Aoyagi, Yasuyuki verfasserin aut Matsuo, Ryota verfasserin aut Enomoto, Tsuyoshi verfasserin aut Matsumoto, Kunio verfasserin aut Nakamura, Toshikazu verfasserin aut Satake, Mitsuo verfasserin aut Ochiai, Atsushi verfasserin aut Ohkohchi, Nobuhiro verfasserin aut Nakajima, Mitsutoshi verfasserin aut Enthalten in Biomedical microdevices Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 9(2006), 1 vom: 11. Nov., Seite 91-99 (DE-627)320433269 (DE-600)2004019-2 1572-8781 nnns volume:9 year:2006 number:1 day:11 month:11 pages:91-99 https://dx.doi.org/10.1007/s10544-006-9011-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE AR 9 2006 1 11 11 91-99 |
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Enthalten in Biomedical microdevices 9(2006), 1 vom: 11. Nov., Seite 91-99 volume:9 year:2006 number:1 day:11 month:11 pages:91-99 |
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Biomedical microdevices |
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Sugiura, Shinji @@aut@@ Oda, Tatsuya @@aut@@ Aoyagi, Yasuyuki @@aut@@ Matsuo, Ryota @@aut@@ Enomoto, Tsuyoshi @@aut@@ Matsumoto, Kunio @@aut@@ Nakamura, Toshikazu @@aut@@ Satake, Mitsuo @@aut@@ Ochiai, Atsushi @@aut@@ Ohkohchi, Nobuhiro @@aut@@ Nakajima, Mitsutoshi @@aut@@ |
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2006-11-11T00:00:00Z |
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Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a $ CaCl_{2} $ solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. 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Sugiura, Shinji |
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Sugiura, Shinji Oda, Tatsuya Aoyagi, Yasuyuki Matsuo, Ryota Enomoto, Tsuyoshi Matsumoto, Kunio Nakamura, Toshikazu Satake, Mitsuo Ochiai, Atsushi Ohkohchi, Nobuhiro Nakajima, Mitsutoshi |
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Elektronische Aufsätze |
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Sugiura, Shinji |
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10.1007/s10544-006-9011-9 |
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microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules |
| title_auth |
Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules |
| abstract |
Abstract Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a $ CaCl_{2} $ solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. The advantages of smaller microcapsules offer new prospects for the advancement of microencapsulation technology. |
| abstractGer |
Abstract Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a $ CaCl_{2} $ solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. The advantages of smaller microcapsules offer new prospects for the advancement of microencapsulation technology. |
| abstract_unstemmed |
Abstract Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a $ CaCl_{2} $ solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. The advantages of smaller microcapsules offer new prospects for the advancement of microencapsulation technology. |
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Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules |
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Oda, Tatsuya Aoyagi, Yasuyuki Matsuo, Ryota Enomoto, Tsuyoshi Matsumoto, Kunio Nakamura, Toshikazu Satake, Mitsuo Ochiai, Atsushi Ohkohchi, Nobuhiro Nakajima, Mitsutoshi |
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Oda, Tatsuya Aoyagi, Yasuyuki Matsuo, Ryota Enomoto, Tsuyoshi Matsumoto, Kunio Nakamura, Toshikazu Satake, Mitsuo Ochiai, Atsushi Ohkohchi, Nobuhiro Nakajima, Mitsutoshi |
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| score |
7.4001417 |