3D cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation
Abstract Tumor-originated and undefined extracellular matrices (ECMs) such as Matrigel™ have been widely used in three-dimensional (3D) cell and tissue culture, but their use is unacceptable in clinical cell therapies. In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kim, Hyo Jeong [verfasserIn] Oh, Dongyeop X. [verfasserIn] Choy, Seunghwan [verfasserIn] Nguyen, Hoang-Linh [verfasserIn] Cha, Hyung Joon [verfasserIn] Hwang, Dong Soo [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Cellulose - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1994, 25(2018), 12 vom: 04. Okt., Seite 7299-7314 |
|---|---|
| Übergeordnetes Werk: |
volume:25 ; year:2018 ; number:12 ; day:04 ; month:10 ; pages:7299-7314 |
| Links: |
|---|
| DOI / URN: |
10.1007/s10570-018-2058-y |
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| Katalog-ID: |
SPR011603623 |
|---|
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| 520 | |a Abstract Tumor-originated and undefined extracellular matrices (ECMs) such as Matrigel™ have been widely used in three-dimensional (3D) cell and tissue culture, but their use is unacceptable in clinical cell therapies. In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great potential as a defined tissue-engineering scaffold, especially for osteoblast culture. The CNF hydrogel showed attractive features as a cell scaffold material. It exhibited a ~ 1.4-fold higher diffusion coefficient (~ 2.98 × $ 10^{−7} $ $ cm^{2} $/s) of macromolecules such as bovine serum albumin than does Matrigel™ (< 2.2 × $ 10^{−7} $ $ cm^{2} $/s) due to the former’s higher porosity (> 95%) and pore size (~ 310.8 μm). Most pre-osteoblast cells that are encapsulated in the CNF hydrogel were immediately locked without sinking by instant hydrogen bond cross-linking between CNFs, whereas cells encapsulated in Matrigel™ sank to the bottom of the scaffold due to the slow sol–gel transition (> 20 min). The elastic modulus of the cell-encapsulated CNF hydrogel could be reinforced by further calcium-mediated cross-linking without cytotoxicity. As a result, the pre-osteoblast cells in the CNF hydrogels were homogeneously distributed in the 3D structure, proliferated for 3 weeks, and successfully differentiated. Overall, CNFs showed that it has potential to be used in tissue engineering as a defined ECM component. Graphical abstract | ||
| 650 | 4 | |a Cellulose nanofibers |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Extracellular matrices |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Matrigel |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Shear-dependent viscosity |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a 3D hydrogel scaffolds |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Oh, Dongyeop X. |e verfasserin |4 aut | |
| 700 | 1 | |a Choy, Seunghwan |e verfasserin |4 aut | |
| 700 | 1 | |a Nguyen, Hoang-Linh |e verfasserin |4 aut | |
| 700 | 1 | |a Cha, Hyung Joon |e verfasserin |4 aut | |
| 700 | 1 | |a Hwang, Dong Soo |e verfasserin |4 aut | |
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10.1007/s10570-018-2058-y doi (DE-627)SPR011603623 (SPR)s10570-018-2058-y-e DE-627 ger DE-627 rakwb eng 540 ASE 35.63 bkl 35.77 bkl Kim, Hyo Jeong verfasserin aut 3D cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Tumor-originated and undefined extracellular matrices (ECMs) such as Matrigel™ have been widely used in three-dimensional (3D) cell and tissue culture, but their use is unacceptable in clinical cell therapies. In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great potential as a defined tissue-engineering scaffold, especially for osteoblast culture. The CNF hydrogel showed attractive features as a cell scaffold material. It exhibited a ~ 1.4-fold higher diffusion coefficient (~ 2.98 × $ 10^{−7} $ $ cm^{2} $/s) of macromolecules such as bovine serum albumin than does Matrigel™ (< 2.2 × $ 10^{−7} $ $ cm^{2} $/s) due to the former’s higher porosity (> 95%) and pore size (~ 310.8 μm). Most pre-osteoblast cells that are encapsulated in the CNF hydrogel were immediately locked without sinking by instant hydrogen bond cross-linking between CNFs, whereas cells encapsulated in Matrigel™ sank to the bottom of the scaffold due to the slow sol–gel transition (> 20 min). The elastic modulus of the cell-encapsulated CNF hydrogel could be reinforced by further calcium-mediated cross-linking without cytotoxicity. As a result, the pre-osteoblast cells in the CNF hydrogels were homogeneously distributed in the 3D structure, proliferated for 3 weeks, and successfully differentiated. Overall, CNFs showed that it has potential to be used in tissue engineering as a defined ECM component. Graphical abstract Cellulose nanofibers (dpeaa)DE-He213 Extracellular matrices (dpeaa)DE-He213 Matrigel (dpeaa)DE-He213 Shear-dependent viscosity (dpeaa)DE-He213 3D hydrogel scaffolds (dpeaa)DE-He213 Oh, Dongyeop X. verfasserin aut Choy, Seunghwan verfasserin aut Nguyen, Hoang-Linh verfasserin aut Cha, Hyung Joon verfasserin aut Hwang, Dong Soo verfasserin aut Enthalten in Cellulose Dordrecht [u.a.] : Springer Science + Business Media B.V, 1994 25(2018), 12 vom: 04. Okt., Seite 7299-7314 (DE-627)306353857 (DE-600)1496831-9 1572-882X nnns volume:25 year:2018 number:12 day:04 month:10 pages:7299-7314 https://dx.doi.org/10.1007/s10570-018-2058-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.63 ASE 35.77 ASE AR 25 2018 12 04 10 7299-7314 |
| spelling |
10.1007/s10570-018-2058-y doi (DE-627)SPR011603623 (SPR)s10570-018-2058-y-e DE-627 ger DE-627 rakwb eng 540 ASE 35.63 bkl 35.77 bkl Kim, Hyo Jeong verfasserin aut 3D cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Tumor-originated and undefined extracellular matrices (ECMs) such as Matrigel™ have been widely used in three-dimensional (3D) cell and tissue culture, but their use is unacceptable in clinical cell therapies. In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great potential as a defined tissue-engineering scaffold, especially for osteoblast culture. The CNF hydrogel showed attractive features as a cell scaffold material. It exhibited a ~ 1.4-fold higher diffusion coefficient (~ 2.98 × $ 10^{−7} $ $ cm^{2} $/s) of macromolecules such as bovine serum albumin than does Matrigel™ (< 2.2 × $ 10^{−7} $ $ cm^{2} $/s) due to the former’s higher porosity (> 95%) and pore size (~ 310.8 μm). Most pre-osteoblast cells that are encapsulated in the CNF hydrogel were immediately locked without sinking by instant hydrogen bond cross-linking between CNFs, whereas cells encapsulated in Matrigel™ sank to the bottom of the scaffold due to the slow sol–gel transition (> 20 min). The elastic modulus of the cell-encapsulated CNF hydrogel could be reinforced by further calcium-mediated cross-linking without cytotoxicity. As a result, the pre-osteoblast cells in the CNF hydrogels were homogeneously distributed in the 3D structure, proliferated for 3 weeks, and successfully differentiated. Overall, CNFs showed that it has potential to be used in tissue engineering as a defined ECM component. Graphical abstract Cellulose nanofibers (dpeaa)DE-He213 Extracellular matrices (dpeaa)DE-He213 Matrigel (dpeaa)DE-He213 Shear-dependent viscosity (dpeaa)DE-He213 3D hydrogel scaffolds (dpeaa)DE-He213 Oh, Dongyeop X. verfasserin aut Choy, Seunghwan verfasserin aut Nguyen, Hoang-Linh verfasserin aut Cha, Hyung Joon verfasserin aut Hwang, Dong Soo verfasserin aut Enthalten in Cellulose Dordrecht [u.a.] : Springer Science + Business Media B.V, 1994 25(2018), 12 vom: 04. Okt., Seite 7299-7314 (DE-627)306353857 (DE-600)1496831-9 1572-882X nnns volume:25 year:2018 number:12 day:04 month:10 pages:7299-7314 https://dx.doi.org/10.1007/s10570-018-2058-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.63 ASE 35.77 ASE AR 25 2018 12 04 10 7299-7314 |
| allfields_unstemmed |
10.1007/s10570-018-2058-y doi (DE-627)SPR011603623 (SPR)s10570-018-2058-y-e DE-627 ger DE-627 rakwb eng 540 ASE 35.63 bkl 35.77 bkl Kim, Hyo Jeong verfasserin aut 3D cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Tumor-originated and undefined extracellular matrices (ECMs) such as Matrigel™ have been widely used in three-dimensional (3D) cell and tissue culture, but their use is unacceptable in clinical cell therapies. In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great potential as a defined tissue-engineering scaffold, especially for osteoblast culture. The CNF hydrogel showed attractive features as a cell scaffold material. It exhibited a ~ 1.4-fold higher diffusion coefficient (~ 2.98 × $ 10^{−7} $ $ cm^{2} $/s) of macromolecules such as bovine serum albumin than does Matrigel™ (< 2.2 × $ 10^{−7} $ $ cm^{2} $/s) due to the former’s higher porosity (> 95%) and pore size (~ 310.8 μm). Most pre-osteoblast cells that are encapsulated in the CNF hydrogel were immediately locked without sinking by instant hydrogen bond cross-linking between CNFs, whereas cells encapsulated in Matrigel™ sank to the bottom of the scaffold due to the slow sol–gel transition (> 20 min). The elastic modulus of the cell-encapsulated CNF hydrogel could be reinforced by further calcium-mediated cross-linking without cytotoxicity. As a result, the pre-osteoblast cells in the CNF hydrogels were homogeneously distributed in the 3D structure, proliferated for 3 weeks, and successfully differentiated. Overall, CNFs showed that it has potential to be used in tissue engineering as a defined ECM component. Graphical abstract Cellulose nanofibers (dpeaa)DE-He213 Extracellular matrices (dpeaa)DE-He213 Matrigel (dpeaa)DE-He213 Shear-dependent viscosity (dpeaa)DE-He213 3D hydrogel scaffolds (dpeaa)DE-He213 Oh, Dongyeop X. verfasserin aut Choy, Seunghwan verfasserin aut Nguyen, Hoang-Linh verfasserin aut Cha, Hyung Joon verfasserin aut Hwang, Dong Soo verfasserin aut Enthalten in Cellulose Dordrecht [u.a.] : Springer Science + Business Media B.V, 1994 25(2018), 12 vom: 04. Okt., Seite 7299-7314 (DE-627)306353857 (DE-600)1496831-9 1572-882X nnns volume:25 year:2018 number:12 day:04 month:10 pages:7299-7314 https://dx.doi.org/10.1007/s10570-018-2058-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.63 ASE 35.77 ASE AR 25 2018 12 04 10 7299-7314 |
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10.1007/s10570-018-2058-y doi (DE-627)SPR011603623 (SPR)s10570-018-2058-y-e DE-627 ger DE-627 rakwb eng 540 ASE 35.63 bkl 35.77 bkl Kim, Hyo Jeong verfasserin aut 3D cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Tumor-originated and undefined extracellular matrices (ECMs) such as Matrigel™ have been widely used in three-dimensional (3D) cell and tissue culture, but their use is unacceptable in clinical cell therapies. In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great potential as a defined tissue-engineering scaffold, especially for osteoblast culture. The CNF hydrogel showed attractive features as a cell scaffold material. It exhibited a ~ 1.4-fold higher diffusion coefficient (~ 2.98 × $ 10^{−7} $ $ cm^{2} $/s) of macromolecules such as bovine serum albumin than does Matrigel™ (< 2.2 × $ 10^{−7} $ $ cm^{2} $/s) due to the former’s higher porosity (> 95%) and pore size (~ 310.8 μm). Most pre-osteoblast cells that are encapsulated in the CNF hydrogel were immediately locked without sinking by instant hydrogen bond cross-linking between CNFs, whereas cells encapsulated in Matrigel™ sank to the bottom of the scaffold due to the slow sol–gel transition (> 20 min). The elastic modulus of the cell-encapsulated CNF hydrogel could be reinforced by further calcium-mediated cross-linking without cytotoxicity. As a result, the pre-osteoblast cells in the CNF hydrogels were homogeneously distributed in the 3D structure, proliferated for 3 weeks, and successfully differentiated. Overall, CNFs showed that it has potential to be used in tissue engineering as a defined ECM component. Graphical abstract Cellulose nanofibers (dpeaa)DE-He213 Extracellular matrices (dpeaa)DE-He213 Matrigel (dpeaa)DE-He213 Shear-dependent viscosity (dpeaa)DE-He213 3D hydrogel scaffolds (dpeaa)DE-He213 Oh, Dongyeop X. verfasserin aut Choy, Seunghwan verfasserin aut Nguyen, Hoang-Linh verfasserin aut Cha, Hyung Joon verfasserin aut Hwang, Dong Soo verfasserin aut Enthalten in Cellulose Dordrecht [u.a.] : Springer Science + Business Media B.V, 1994 25(2018), 12 vom: 04. Okt., Seite 7299-7314 (DE-627)306353857 (DE-600)1496831-9 1572-882X nnns volume:25 year:2018 number:12 day:04 month:10 pages:7299-7314 https://dx.doi.org/10.1007/s10570-018-2058-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.63 ASE 35.77 ASE AR 25 2018 12 04 10 7299-7314 |
| allfieldsSound |
10.1007/s10570-018-2058-y doi (DE-627)SPR011603623 (SPR)s10570-018-2058-y-e DE-627 ger DE-627 rakwb eng 540 ASE 35.63 bkl 35.77 bkl Kim, Hyo Jeong verfasserin aut 3D cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Tumor-originated and undefined extracellular matrices (ECMs) such as Matrigel™ have been widely used in three-dimensional (3D) cell and tissue culture, but their use is unacceptable in clinical cell therapies. In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great potential as a defined tissue-engineering scaffold, especially for osteoblast culture. The CNF hydrogel showed attractive features as a cell scaffold material. It exhibited a ~ 1.4-fold higher diffusion coefficient (~ 2.98 × $ 10^{−7} $ $ cm^{2} $/s) of macromolecules such as bovine serum albumin than does Matrigel™ (< 2.2 × $ 10^{−7} $ $ cm^{2} $/s) due to the former’s higher porosity (> 95%) and pore size (~ 310.8 μm). Most pre-osteoblast cells that are encapsulated in the CNF hydrogel were immediately locked without sinking by instant hydrogen bond cross-linking between CNFs, whereas cells encapsulated in Matrigel™ sank to the bottom of the scaffold due to the slow sol–gel transition (> 20 min). The elastic modulus of the cell-encapsulated CNF hydrogel could be reinforced by further calcium-mediated cross-linking without cytotoxicity. As a result, the pre-osteoblast cells in the CNF hydrogels were homogeneously distributed in the 3D structure, proliferated for 3 weeks, and successfully differentiated. Overall, CNFs showed that it has potential to be used in tissue engineering as a defined ECM component. Graphical abstract Cellulose nanofibers (dpeaa)DE-He213 Extracellular matrices (dpeaa)DE-He213 Matrigel (dpeaa)DE-He213 Shear-dependent viscosity (dpeaa)DE-He213 3D hydrogel scaffolds (dpeaa)DE-He213 Oh, Dongyeop X. verfasserin aut Choy, Seunghwan verfasserin aut Nguyen, Hoang-Linh verfasserin aut Cha, Hyung Joon verfasserin aut Hwang, Dong Soo verfasserin aut Enthalten in Cellulose Dordrecht [u.a.] : Springer Science + Business Media B.V, 1994 25(2018), 12 vom: 04. Okt., Seite 7299-7314 (DE-627)306353857 (DE-600)1496831-9 1572-882X nnns volume:25 year:2018 number:12 day:04 month:10 pages:7299-7314 https://dx.doi.org/10.1007/s10570-018-2058-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.63 ASE 35.77 ASE AR 25 2018 12 04 10 7299-7314 |
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English |
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Enthalten in Cellulose 25(2018), 12 vom: 04. Okt., Seite 7299-7314 volume:25 year:2018 number:12 day:04 month:10 pages:7299-7314 |
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Enthalten in Cellulose 25(2018), 12 vom: 04. Okt., Seite 7299-7314 volume:25 year:2018 number:12 day:04 month:10 pages:7299-7314 |
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Cellulose nanofibers Extracellular matrices Matrigel Shear-dependent viscosity 3D hydrogel scaffolds |
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Kim, Hyo Jeong @@aut@@ Oh, Dongyeop X. @@aut@@ Choy, Seunghwan @@aut@@ Nguyen, Hoang-Linh @@aut@@ Cha, Hyung Joon @@aut@@ Hwang, Dong Soo @@aut@@ |
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2018-10-04T00:00:00Z |
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In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great potential as a defined tissue-engineering scaffold, especially for osteoblast culture. The CNF hydrogel showed attractive features as a cell scaffold material. It exhibited a ~ 1.4-fold higher diffusion coefficient (~ 2.98 × $ 10^{−7} $ $ cm^{2} $/s) of macromolecules such as bovine serum albumin than does Matrigel™ (< 2.2 × $ 10^{−7} $ $ cm^{2} $/s) due to the former’s higher porosity (> 95%) and pore size (~ 310.8 μm). Most pre-osteoblast cells that are encapsulated in the CNF hydrogel were immediately locked without sinking by instant hydrogen bond cross-linking between CNFs, whereas cells encapsulated in Matrigel™ sank to the bottom of the scaffold due to the slow sol–gel transition (> 20 min). The elastic modulus of the cell-encapsulated CNF hydrogel could be reinforced by further calcium-mediated cross-linking without cytotoxicity. As a result, the pre-osteoblast cells in the CNF hydrogels were homogeneously distributed in the 3D structure, proliferated for 3 weeks, and successfully differentiated. Overall, CNFs showed that it has potential to be used in tissue engineering as a defined ECM component. 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Kim, Hyo Jeong |
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Kim, Hyo Jeong ddc 540 bkl 35.63 bkl 35.77 misc Cellulose nanofibers misc Extracellular matrices misc Matrigel misc Shear-dependent viscosity misc 3D hydrogel scaffolds 3D cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation |
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540 ASE 35.63 bkl 35.77 bkl 3D cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation Cellulose nanofibers (dpeaa)DE-He213 Extracellular matrices (dpeaa)DE-He213 Matrigel (dpeaa)DE-He213 Shear-dependent viscosity (dpeaa)DE-He213 3D hydrogel scaffolds (dpeaa)DE-He213 |
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3d cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation |
| title_auth |
3D cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation |
| abstract |
Abstract Tumor-originated and undefined extracellular matrices (ECMs) such as Matrigel™ have been widely used in three-dimensional (3D) cell and tissue culture, but their use is unacceptable in clinical cell therapies. In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great potential as a defined tissue-engineering scaffold, especially for osteoblast culture. The CNF hydrogel showed attractive features as a cell scaffold material. It exhibited a ~ 1.4-fold higher diffusion coefficient (~ 2.98 × $ 10^{−7} $ $ cm^{2} $/s) of macromolecules such as bovine serum albumin than does Matrigel™ (< 2.2 × $ 10^{−7} $ $ cm^{2} $/s) due to the former’s higher porosity (> 95%) and pore size (~ 310.8 μm). Most pre-osteoblast cells that are encapsulated in the CNF hydrogel were immediately locked without sinking by instant hydrogen bond cross-linking between CNFs, whereas cells encapsulated in Matrigel™ sank to the bottom of the scaffold due to the slow sol–gel transition (> 20 min). The elastic modulus of the cell-encapsulated CNF hydrogel could be reinforced by further calcium-mediated cross-linking without cytotoxicity. As a result, the pre-osteoblast cells in the CNF hydrogels were homogeneously distributed in the 3D structure, proliferated for 3 weeks, and successfully differentiated. Overall, CNFs showed that it has potential to be used in tissue engineering as a defined ECM component. Graphical abstract |
| abstractGer |
Abstract Tumor-originated and undefined extracellular matrices (ECMs) such as Matrigel™ have been widely used in three-dimensional (3D) cell and tissue culture, but their use is unacceptable in clinical cell therapies. In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great potential as a defined tissue-engineering scaffold, especially for osteoblast culture. The CNF hydrogel showed attractive features as a cell scaffold material. It exhibited a ~ 1.4-fold higher diffusion coefficient (~ 2.98 × $ 10^{−7} $ $ cm^{2} $/s) of macromolecules such as bovine serum albumin than does Matrigel™ (< 2.2 × $ 10^{−7} $ $ cm^{2} $/s) due to the former’s higher porosity (> 95%) and pore size (~ 310.8 μm). Most pre-osteoblast cells that are encapsulated in the CNF hydrogel were immediately locked without sinking by instant hydrogen bond cross-linking between CNFs, whereas cells encapsulated in Matrigel™ sank to the bottom of the scaffold due to the slow sol–gel transition (> 20 min). The elastic modulus of the cell-encapsulated CNF hydrogel could be reinforced by further calcium-mediated cross-linking without cytotoxicity. As a result, the pre-osteoblast cells in the CNF hydrogels were homogeneously distributed in the 3D structure, proliferated for 3 weeks, and successfully differentiated. Overall, CNFs showed that it has potential to be used in tissue engineering as a defined ECM component. Graphical abstract |
| abstract_unstemmed |
Abstract Tumor-originated and undefined extracellular matrices (ECMs) such as Matrigel™ have been widely used in three-dimensional (3D) cell and tissue culture, but their use is unacceptable in clinical cell therapies. In this study, we proposed a 3D cellulose nanofiber (CNF) hydrogel that has great potential as a defined tissue-engineering scaffold, especially for osteoblast culture. The CNF hydrogel showed attractive features as a cell scaffold material. It exhibited a ~ 1.4-fold higher diffusion coefficient (~ 2.98 × $ 10^{−7} $ $ cm^{2} $/s) of macromolecules such as bovine serum albumin than does Matrigel™ (< 2.2 × $ 10^{−7} $ $ cm^{2} $/s) due to the former’s higher porosity (> 95%) and pore size (~ 310.8 μm). Most pre-osteoblast cells that are encapsulated in the CNF hydrogel were immediately locked without sinking by instant hydrogen bond cross-linking between CNFs, whereas cells encapsulated in Matrigel™ sank to the bottom of the scaffold due to the slow sol–gel transition (> 20 min). The elastic modulus of the cell-encapsulated CNF hydrogel could be reinforced by further calcium-mediated cross-linking without cytotoxicity. As a result, the pre-osteoblast cells in the CNF hydrogels were homogeneously distributed in the 3D structure, proliferated for 3 weeks, and successfully differentiated. Overall, CNFs showed that it has potential to be used in tissue engineering as a defined ECM component. Graphical abstract |
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| title_short |
3D cellulose nanofiber scaffold with homogeneous cell population and long-term proliferation |
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https://dx.doi.org/10.1007/s10570-018-2058-y |
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Oh, Dongyeop X. Choy, Seunghwan Nguyen, Hoang-Linh Cha, Hyung Joon Hwang, Dong Soo |
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| up_date |
2024-07-03T23:35:51.562Z |
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| score |
7.398777 |