Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine
Abstract Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFL...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Krebelj, Anastazija Jež [verfasserIn] Čepin, Urška [verfasserIn] Ravnikar, Maja [verfasserIn] Novak, Maruša Pompe [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: European journal of plant pathology - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895, 142(2015), 1 vom: 09. Feb., Seite 159-171 |
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| Übergeordnetes Werk: |
volume:142 ; year:2015 ; number:1 ; day:09 ; month:02 ; pages:159-171 |
| Links: |
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| DOI / URN: |
10.1007/s10658-015-0600-4 |
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| Katalog-ID: |
SPR012176079 |
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| 520 | |a Abstract Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. Additionally, different GFLV titres were shown in five different cultivars and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptoms’ appearance and GFLV titres was analysed. | ||
| 650 | 4 | |a Grapevine fanleaf virus |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a GFLV |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Grapevine disease |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Virus titre |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Distribution |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Fluctuation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a ELISA |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a qPCR |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Čepin, Urška |e verfasserin |4 aut | |
| 700 | 1 | |a Ravnikar, Maja |e verfasserin |4 aut | |
| 700 | 1 | |a Novak, Maruša Pompe |e verfasserin |4 aut | |
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10.1007/s10658-015-0600-4 doi (DE-627)SPR012176079 (SPR)s10658-015-0600-4-e DE-627 ger DE-627 rakwb eng 580 630 640 ASE 48.54 bkl Krebelj, Anastazija Jež verfasserin aut Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. Additionally, different GFLV titres were shown in five different cultivars and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptoms’ appearance and GFLV titres was analysed. Grapevine fanleaf virus (dpeaa)DE-He213 GFLV (dpeaa)DE-He213 Grapevine disease (dpeaa)DE-He213 Virus titre (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Fluctuation (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Čepin, Urška verfasserin aut Ravnikar, Maja verfasserin aut Novak, Maruša Pompe verfasserin aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 142(2015), 1 vom: 09. Feb., Seite 159-171 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:142 year:2015 number:1 day:09 month:02 pages:159-171 https://dx.doi.org/10.1007/s10658-015-0600-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 142 2015 1 09 02 159-171 |
| spelling |
10.1007/s10658-015-0600-4 doi (DE-627)SPR012176079 (SPR)s10658-015-0600-4-e DE-627 ger DE-627 rakwb eng 580 630 640 ASE 48.54 bkl Krebelj, Anastazija Jež verfasserin aut Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. Additionally, different GFLV titres were shown in five different cultivars and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptoms’ appearance and GFLV titres was analysed. Grapevine fanleaf virus (dpeaa)DE-He213 GFLV (dpeaa)DE-He213 Grapevine disease (dpeaa)DE-He213 Virus titre (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Fluctuation (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Čepin, Urška verfasserin aut Ravnikar, Maja verfasserin aut Novak, Maruša Pompe verfasserin aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 142(2015), 1 vom: 09. Feb., Seite 159-171 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:142 year:2015 number:1 day:09 month:02 pages:159-171 https://dx.doi.org/10.1007/s10658-015-0600-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 142 2015 1 09 02 159-171 |
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10.1007/s10658-015-0600-4 doi (DE-627)SPR012176079 (SPR)s10658-015-0600-4-e DE-627 ger DE-627 rakwb eng 580 630 640 ASE 48.54 bkl Krebelj, Anastazija Jež verfasserin aut Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. Additionally, different GFLV titres were shown in five different cultivars and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptoms’ appearance and GFLV titres was analysed. Grapevine fanleaf virus (dpeaa)DE-He213 GFLV (dpeaa)DE-He213 Grapevine disease (dpeaa)DE-He213 Virus titre (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Fluctuation (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Čepin, Urška verfasserin aut Ravnikar, Maja verfasserin aut Novak, Maruša Pompe verfasserin aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 142(2015), 1 vom: 09. Feb., Seite 159-171 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:142 year:2015 number:1 day:09 month:02 pages:159-171 https://dx.doi.org/10.1007/s10658-015-0600-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 142 2015 1 09 02 159-171 |
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10.1007/s10658-015-0600-4 doi (DE-627)SPR012176079 (SPR)s10658-015-0600-4-e DE-627 ger DE-627 rakwb eng 580 630 640 ASE 48.54 bkl Krebelj, Anastazija Jež verfasserin aut Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. Additionally, different GFLV titres were shown in five different cultivars and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptoms’ appearance and GFLV titres was analysed. Grapevine fanleaf virus (dpeaa)DE-He213 GFLV (dpeaa)DE-He213 Grapevine disease (dpeaa)DE-He213 Virus titre (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Fluctuation (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Čepin, Urška verfasserin aut Ravnikar, Maja verfasserin aut Novak, Maruša Pompe verfasserin aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 142(2015), 1 vom: 09. Feb., Seite 159-171 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:142 year:2015 number:1 day:09 month:02 pages:159-171 https://dx.doi.org/10.1007/s10658-015-0600-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 142 2015 1 09 02 159-171 |
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10.1007/s10658-015-0600-4 doi (DE-627)SPR012176079 (SPR)s10658-015-0600-4-e DE-627 ger DE-627 rakwb eng 580 630 640 ASE 48.54 bkl Krebelj, Anastazija Jež verfasserin aut Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. Additionally, different GFLV titres were shown in five different cultivars and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptoms’ appearance and GFLV titres was analysed. Grapevine fanleaf virus (dpeaa)DE-He213 GFLV (dpeaa)DE-He213 Grapevine disease (dpeaa)DE-He213 Virus titre (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Fluctuation (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 Čepin, Urška verfasserin aut Ravnikar, Maja verfasserin aut Novak, Maruša Pompe verfasserin aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 142(2015), 1 vom: 09. Feb., Seite 159-171 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:142 year:2015 number:1 day:09 month:02 pages:159-171 https://dx.doi.org/10.1007/s10658-015-0600-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 142 2015 1 09 02 159-171 |
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English |
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Enthalten in European journal of plant pathology 142(2015), 1 vom: 09. Feb., Seite 159-171 volume:142 year:2015 number:1 day:09 month:02 pages:159-171 |
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Enthalten in European journal of plant pathology 142(2015), 1 vom: 09. Feb., Seite 159-171 volume:142 year:2015 number:1 day:09 month:02 pages:159-171 |
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Grapevine fanleaf virus GFLV Grapevine disease Virus titre Distribution Fluctuation ELISA qPCR |
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European journal of plant pathology |
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Krebelj, Anastazija Jež @@aut@@ Čepin, Urška @@aut@@ Ravnikar, Maja @@aut@@ Novak, Maruša Pompe @@aut@@ |
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The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. 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|
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Krebelj, Anastazija Jež |
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Krebelj, Anastazija Jež ddc 580 bkl 48.54 misc Grapevine fanleaf virus misc GFLV misc Grapevine disease misc Virus titre misc Distribution misc Fluctuation misc ELISA misc qPCR Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine |
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580 630 640 ASE 48.54 bkl Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine Grapevine fanleaf virus (dpeaa)DE-He213 GFLV (dpeaa)DE-He213 Grapevine disease (dpeaa)DE-He213 Virus titre (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Fluctuation (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 qPCR (dpeaa)DE-He213 |
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ddc 580 bkl 48.54 misc Grapevine fanleaf virus misc GFLV misc Grapevine disease misc Virus titre misc Distribution misc Fluctuation misc ELISA misc qPCR |
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ddc 580 bkl 48.54 misc Grapevine fanleaf virus misc GFLV misc Grapevine disease misc Virus titre misc Distribution misc Fluctuation misc ELISA misc qPCR |
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Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine |
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Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine |
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Krebelj, Anastazija Jež |
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European journal of plant pathology |
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Krebelj, Anastazija Jež Čepin, Urška Ravnikar, Maja Novak, Maruša Pompe |
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Krebelj, Anastazija Jež |
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10.1007/s10658-015-0600-4 |
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verfasserin |
| title_sort |
spatio-temporal distribution of grapevine fanleaf virus (gflv) in grapevine |
| title_auth |
Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine |
| abstract |
Abstract Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. Additionally, different GFLV titres were shown in five different cultivars and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptoms’ appearance and GFLV titres was analysed. |
| abstractGer |
Abstract Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. Additionally, different GFLV titres were shown in five different cultivars and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptoms’ appearance and GFLV titres was analysed. |
| abstract_unstemmed |
Abstract Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. Additionally, different GFLV titres were shown in five different cultivars and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptoms’ appearance and GFLV titres was analysed. |
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| container_issue |
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| title_short |
Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine |
| url |
https://dx.doi.org/10.1007/s10658-015-0600-4 |
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| author2 |
Čepin, Urška Ravnikar, Maja Novak, Maruša Pompe |
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Čepin, Urška Ravnikar, Maja Novak, Maruša Pompe |
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| doi_str |
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| up_date |
2024-07-04T02:06:13.009Z |
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| score |
7.402915 |