Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response

The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS)...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Li, L. [verfasserIn] Cao, X. [verfasserIn] Zhou, Zh. [verfasserIn] Zhang, S. [verfasserIn] Pan, H. [verfasserIn] Chen, J. [verfasserIn] Xu, J. [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	fluorescent sensor genetically encoded pH indicator Trp-Ala-Ala-Ser Trp-Glu-Ala-Ser Trp-Trp-Ala-Ser

Übergeordnetes Werk:	Enthalten in: Journal of applied spectroscopy - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1965, 84(2017), 4 vom: Sept., Seite 633-638
Übergeordnetes Werk:	volume:84 ; year:2017 ; number:4 ; month:09 ; pages:633-638

Links:	Volltext

DOI / URN:	10.1007/s10812-017-0521-4

Katalog-ID:	SPR01351038X

Internformat


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520			\|a The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS) and Trp-Glu-Ala-Ser (WEAS), have higher fluorescence quantum yields, longer fluorescence lifetimes, and more sensitive pH responses. As novel genetically encoded pH indicators, the N-terminal Trp-Ala and Trp-Glu could be fused to proteins for monitoring the environmental pH values during the studies of functional proteins.
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650		4	\|a genetically encoded pH indicator \|7 (dpeaa)DE-He213
650		4	\|a Trp-Ala-Ala-Ser \|7 (dpeaa)DE-He213
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912			\|a GBV_USEFLAG_A
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912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
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Indexfelder

author_variant	l l ll x c xc z z zz s z sz h p hp j c jc j x jx
matchkey_str	article:15738647:2017----::loecneyaisftriatytfnrsdeipl
hierarchy_sort_str	2017
bklnumber	33.00
publishDate	2017
allfields	10.1007/s10812-017-0521-4 doi (DE-627)SPR01351038X (SPR)s10812-017-0521-4-e DE-627 ger DE-627 rakwb eng 530 ASE 33.00 bkl Li, L. verfasserin aut Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS) and Trp-Glu-Ala-Ser (WEAS), have higher fluorescence quantum yields, longer fluorescence lifetimes, and more sensitive pH responses. As novel genetically encoded pH indicators, the N-terminal Trp-Ala and Trp-Glu could be fused to proteins for monitoring the environmental pH values during the studies of functional proteins. fluorescent sensor (dpeaa)DE-He213 genetically encoded pH indicator (dpeaa)DE-He213 Trp-Ala-Ala-Ser (dpeaa)DE-He213 Trp-Glu-Ala-Ser (dpeaa)DE-He213 Trp-Trp-Ala-Ser (dpeaa)DE-He213 Cao, X. verfasserin aut Zhou, Zh. verfasserin aut Zhang, S. verfasserin aut Pan, H. verfasserin aut Chen, J. verfasserin aut Xu, J. verfasserin aut Enthalten in Journal of applied spectroscopy Dordrecht [u.a.] : Springer Science + Business Media B.V, 1965 84(2017), 4 vom: Sept., Seite 633-638 (DE-627)325609918 (DE-600)2037920-1 1573-8647 nnns volume:84 year:2017 number:4 month:09 pages:633-638 https://dx.doi.org/10.1007/s10812-017-0521-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 33.00 ASE AR 84 2017 4 09 633-638
spelling	10.1007/s10812-017-0521-4 doi (DE-627)SPR01351038X (SPR)s10812-017-0521-4-e DE-627 ger DE-627 rakwb eng 530 ASE 33.00 bkl Li, L. verfasserin aut Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS) and Trp-Glu-Ala-Ser (WEAS), have higher fluorescence quantum yields, longer fluorescence lifetimes, and more sensitive pH responses. As novel genetically encoded pH indicators, the N-terminal Trp-Ala and Trp-Glu could be fused to proteins for monitoring the environmental pH values during the studies of functional proteins. fluorescent sensor (dpeaa)DE-He213 genetically encoded pH indicator (dpeaa)DE-He213 Trp-Ala-Ala-Ser (dpeaa)DE-He213 Trp-Glu-Ala-Ser (dpeaa)DE-He213 Trp-Trp-Ala-Ser (dpeaa)DE-He213 Cao, X. verfasserin aut Zhou, Zh. verfasserin aut Zhang, S. verfasserin aut Pan, H. verfasserin aut Chen, J. verfasserin aut Xu, J. verfasserin aut Enthalten in Journal of applied spectroscopy Dordrecht [u.a.] : Springer Science + Business Media B.V, 1965 84(2017), 4 vom: Sept., Seite 633-638 (DE-627)325609918 (DE-600)2037920-1 1573-8647 nnns volume:84 year:2017 number:4 month:09 pages:633-638 https://dx.doi.org/10.1007/s10812-017-0521-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 33.00 ASE AR 84 2017 4 09 633-638
allfields_unstemmed	10.1007/s10812-017-0521-4 doi (DE-627)SPR01351038X (SPR)s10812-017-0521-4-e DE-627 ger DE-627 rakwb eng 530 ASE 33.00 bkl Li, L. verfasserin aut Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS) and Trp-Glu-Ala-Ser (WEAS), have higher fluorescence quantum yields, longer fluorescence lifetimes, and more sensitive pH responses. As novel genetically encoded pH indicators, the N-terminal Trp-Ala and Trp-Glu could be fused to proteins for monitoring the environmental pH values during the studies of functional proteins. fluorescent sensor (dpeaa)DE-He213 genetically encoded pH indicator (dpeaa)DE-He213 Trp-Ala-Ala-Ser (dpeaa)DE-He213 Trp-Glu-Ala-Ser (dpeaa)DE-He213 Trp-Trp-Ala-Ser (dpeaa)DE-He213 Cao, X. verfasserin aut Zhou, Zh. verfasserin aut Zhang, S. verfasserin aut Pan, H. verfasserin aut Chen, J. verfasserin aut Xu, J. verfasserin aut Enthalten in Journal of applied spectroscopy Dordrecht [u.a.] : Springer Science + Business Media B.V, 1965 84(2017), 4 vom: Sept., Seite 633-638 (DE-627)325609918 (DE-600)2037920-1 1573-8647 nnns volume:84 year:2017 number:4 month:09 pages:633-638 https://dx.doi.org/10.1007/s10812-017-0521-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 33.00 ASE AR 84 2017 4 09 633-638
allfieldsGer	10.1007/s10812-017-0521-4 doi (DE-627)SPR01351038X (SPR)s10812-017-0521-4-e DE-627 ger DE-627 rakwb eng 530 ASE 33.00 bkl Li, L. verfasserin aut Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS) and Trp-Glu-Ala-Ser (WEAS), have higher fluorescence quantum yields, longer fluorescence lifetimes, and more sensitive pH responses. As novel genetically encoded pH indicators, the N-terminal Trp-Ala and Trp-Glu could be fused to proteins for monitoring the environmental pH values during the studies of functional proteins. fluorescent sensor (dpeaa)DE-He213 genetically encoded pH indicator (dpeaa)DE-He213 Trp-Ala-Ala-Ser (dpeaa)DE-He213 Trp-Glu-Ala-Ser (dpeaa)DE-He213 Trp-Trp-Ala-Ser (dpeaa)DE-He213 Cao, X. verfasserin aut Zhou, Zh. verfasserin aut Zhang, S. verfasserin aut Pan, H. verfasserin aut Chen, J. verfasserin aut Xu, J. verfasserin aut Enthalten in Journal of applied spectroscopy Dordrecht [u.a.] : Springer Science + Business Media B.V, 1965 84(2017), 4 vom: Sept., Seite 633-638 (DE-627)325609918 (DE-600)2037920-1 1573-8647 nnns volume:84 year:2017 number:4 month:09 pages:633-638 https://dx.doi.org/10.1007/s10812-017-0521-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 33.00 ASE AR 84 2017 4 09 633-638
allfieldsSound	10.1007/s10812-017-0521-4 doi (DE-627)SPR01351038X (SPR)s10812-017-0521-4-e DE-627 ger DE-627 rakwb eng 530 ASE 33.00 bkl Li, L. verfasserin aut Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS) and Trp-Glu-Ala-Ser (WEAS), have higher fluorescence quantum yields, longer fluorescence lifetimes, and more sensitive pH responses. As novel genetically encoded pH indicators, the N-terminal Trp-Ala and Trp-Glu could be fused to proteins for monitoring the environmental pH values during the studies of functional proteins. fluorescent sensor (dpeaa)DE-He213 genetically encoded pH indicator (dpeaa)DE-He213 Trp-Ala-Ala-Ser (dpeaa)DE-He213 Trp-Glu-Ala-Ser (dpeaa)DE-He213 Trp-Trp-Ala-Ser (dpeaa)DE-He213 Cao, X. verfasserin aut Zhou, Zh. verfasserin aut Zhang, S. verfasserin aut Pan, H. verfasserin aut Chen, J. verfasserin aut Xu, J. verfasserin aut Enthalten in Journal of applied spectroscopy Dordrecht [u.a.] : Springer Science + Business Media B.V, 1965 84(2017), 4 vom: Sept., Seite 633-638 (DE-627)325609918 (DE-600)2037920-1 1573-8647 nnns volume:84 year:2017 number:4 month:09 pages:633-638 https://dx.doi.org/10.1007/s10812-017-0521-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 33.00 ASE AR 84 2017 4 09 633-638
language	English
source	Enthalten in Journal of applied spectroscopy 84(2017), 4 vom: Sept., Seite 633-638 volume:84 year:2017 number:4 month:09 pages:633-638
sourceStr	Enthalten in Journal of applied spectroscopy 84(2017), 4 vom: Sept., Seite 633-638 volume:84 year:2017 number:4 month:09 pages:633-638
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	fluorescent sensor genetically encoded pH indicator Trp-Ala-Ala-Ser Trp-Glu-Ala-Ser Trp-Trp-Ala-Ser
dewey-raw	530
isfreeaccess_bool	false
container_title	Journal of applied spectroscopy
authorswithroles_txt_mv	Li, L. @@aut@@ Cao, X. @@aut@@ Zhou, Zh. @@aut@@ Zhang, S. @@aut@@ Pan, H. @@aut@@ Chen, J. @@aut@@ Xu, J. @@aut@@
publishDateDaySort_date	2017-09-01T00:00:00Z
hierarchy_top_id	325609918
dewey-sort	3530
id	SPR01351038X
language_de	englisch
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author	Li, L.
spellingShingle	Li, L. ddc 530 bkl 33.00 misc fluorescent sensor misc genetically encoded pH indicator misc Trp-Ala-Ala-Ser misc Trp-Glu-Ala-Ser misc Trp-Trp-Ala-Ser Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response
authorStr	Li, L.
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topic_title	530 ASE 33.00 bkl Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response fluorescent sensor (dpeaa)DE-He213 genetically encoded pH indicator (dpeaa)DE-He213 Trp-Ala-Ala-Ser (dpeaa)DE-He213 Trp-Glu-Ala-Ser (dpeaa)DE-He213 Trp-Trp-Ala-Ser (dpeaa)DE-He213
topic	ddc 530 bkl 33.00 misc fluorescent sensor misc genetically encoded pH indicator misc Trp-Ala-Ala-Ser misc Trp-Glu-Ala-Ser misc Trp-Trp-Ala-Ser
topic_unstemmed	ddc 530 bkl 33.00 misc fluorescent sensor misc genetically encoded pH indicator misc Trp-Ala-Ala-Ser misc Trp-Glu-Ala-Ser misc Trp-Trp-Ala-Ser
topic_browse	ddc 530 bkl 33.00 misc fluorescent sensor misc genetically encoded pH indicator misc Trp-Ala-Ala-Ser misc Trp-Glu-Ala-Ser misc Trp-Trp-Ala-Ser
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title	Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response
ctrlnum	(DE-627)SPR01351038X (SPR)s10812-017-0521-4-e
title_full	Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response
author_sort	Li, L.
journal	Journal of applied spectroscopy
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author_browse	Li, L. Cao, X. Zhou, Zh. Zhang, S. Pan, H. Chen, J. Xu, J.
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author-letter	Li, L.
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title_sort	fluorescence dynamics of n-terminal tryptofan-x residues in polypeptide: ph response
title_auth	Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response
abstract	The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS) and Trp-Glu-Ala-Ser (WEAS), have higher fluorescence quantum yields, longer fluorescence lifetimes, and more sensitive pH responses. As novel genetically encoded pH indicators, the N-terminal Trp-Ala and Trp-Glu could be fused to proteins for monitoring the environmental pH values during the studies of functional proteins.
abstractGer	The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS) and Trp-Glu-Ala-Ser (WEAS), have higher fluorescence quantum yields, longer fluorescence lifetimes, and more sensitive pH responses. As novel genetically encoded pH indicators, the N-terminal Trp-Ala and Trp-Glu could be fused to proteins for monitoring the environmental pH values during the studies of functional proteins.
abstract_unstemmed	The influence of the second amino acid on the pH response of the genetically encoded pH indicators is studied. The second Trp has been substituted by Ala and Glu to reduce self-quenching. Compared with the previously reported Trp-Trp-Ala-Ser (WWAS), the two new tetrapeptides, Trp-Ala-Ala-Ser (WAAS) and Trp-Glu-Ala-Ser (WEAS), have higher fluorescence quantum yields, longer fluorescence lifetimes, and more sensitive pH responses. As novel genetically encoded pH indicators, the N-terminal Trp-Ala and Trp-Glu could be fused to proteins for monitoring the environmental pH values during the studies of functional proteins.
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container_issue	4
title_short	Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response
url	https://dx.doi.org/10.1007/s10812-017-0521-4
remote_bool	true
author2	Cao, X. Zhou, Zh Zhang, S. Pan, H. Chen, J. Xu, J.
author2Str	Cao, X. Zhou, Zh Zhang, S. Pan, H. Chen, J. Xu, J.
ppnlink	325609918
mediatype_str_mv	c
isOA_txt	false
hochschulschrift_bool	false
doi_str	10.1007/s10812-017-0521-4
up_date	2024-07-03T20:13:47.498Z
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Fluorescence Dynamics of N-Terminal Tryptofan-X Residues in Polypeptide: pH Response

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