Effect of intra-ovarian injection of mesenchymal stem cells in aged mares
Purpose This study aims to determine if intra-ovarian injection of bone marrow–derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicl...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Grady, Sicilia T. [verfasserIn] Watts, Ashlee E. [verfasserIn] Thompson, James A. [verfasserIn] Penedo, M. Cecilia T. [verfasserIn] Konganti, Kranti [verfasserIn] Hinrichs, Katrin [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of assisted reproduction and genetics - Dordrecht [u.a.] : Springer Science + Business Media B.V., 1984, 36(2018), 3 vom: 23. Nov., Seite 543-556 |
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| Übergeordnetes Werk: |
volume:36 ; year:2018 ; number:3 ; day:23 ; month:11 ; pages:543-556 |
| Links: |
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| DOI / URN: |
10.1007/s10815-018-1371-6 |
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| Katalog-ID: |
SPR013540289 |
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| 520 | |a Purpose This study aims to determine if intra-ovarian injection of bone marrow–derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Results Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries. Conclusions Intra-ovarian injection of bone marrow–derived MSCs altered gene expression but did not improve ovarian function in aged mares. | ||
| 650 | 4 | |a Aging |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Anti-Müllerian hormone |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Equids |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Fertility |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Follicle-stimulating hormone |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Follicular development |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Oocyte |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Ovary |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Stem cells |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Watts, Ashlee E. |e verfasserin |4 aut | |
| 700 | 1 | |a Thompson, James A. |e verfasserin |4 aut | |
| 700 | 1 | |a Penedo, M. Cecilia T. |e verfasserin |4 aut | |
| 700 | 1 | |a Konganti, Kranti |e verfasserin |4 aut | |
| 700 | 1 | |a Hinrichs, Katrin |e verfasserin |4 aut | |
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10.1007/s10815-018-1371-6 doi (DE-627)SPR013540289 (SPR)s10815-018-1371-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.92 bkl Grady, Sicilia T. verfasserin aut Effect of intra-ovarian injection of mesenchymal stem cells in aged mares 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose This study aims to determine if intra-ovarian injection of bone marrow–derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Results Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries. Conclusions Intra-ovarian injection of bone marrow–derived MSCs altered gene expression but did not improve ovarian function in aged mares. Aging (dpeaa)DE-He213 Anti-Müllerian hormone (dpeaa)DE-He213 Equids (dpeaa)DE-He213 Fertility (dpeaa)DE-He213 Follicle-stimulating hormone (dpeaa)DE-He213 Follicular development (dpeaa)DE-He213 Oocyte (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Stem cells (dpeaa)DE-He213 Watts, Ashlee E. verfasserin aut Thompson, James A. verfasserin aut Penedo, M. Cecilia T. verfasserin aut Konganti, Kranti verfasserin aut Hinrichs, Katrin verfasserin aut Enthalten in Journal of assisted reproduction and genetics Dordrecht [u.a.] : Springer Science + Business Media B.V., 1984 36(2018), 3 vom: 23. Nov., Seite 543-556 (DE-627)320573060 (DE-600)2016722-2 1573-7330 nnns volume:36 year:2018 number:3 day:23 month:11 pages:543-556 https://dx.doi.org/10.1007/s10815-018-1371-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 44.92 ASE AR 36 2018 3 23 11 543-556 |
| spelling |
10.1007/s10815-018-1371-6 doi (DE-627)SPR013540289 (SPR)s10815-018-1371-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.92 bkl Grady, Sicilia T. verfasserin aut Effect of intra-ovarian injection of mesenchymal stem cells in aged mares 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose This study aims to determine if intra-ovarian injection of bone marrow–derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Results Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries. Conclusions Intra-ovarian injection of bone marrow–derived MSCs altered gene expression but did not improve ovarian function in aged mares. Aging (dpeaa)DE-He213 Anti-Müllerian hormone (dpeaa)DE-He213 Equids (dpeaa)DE-He213 Fertility (dpeaa)DE-He213 Follicle-stimulating hormone (dpeaa)DE-He213 Follicular development (dpeaa)DE-He213 Oocyte (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Stem cells (dpeaa)DE-He213 Watts, Ashlee E. verfasserin aut Thompson, James A. verfasserin aut Penedo, M. Cecilia T. verfasserin aut Konganti, Kranti verfasserin aut Hinrichs, Katrin verfasserin aut Enthalten in Journal of assisted reproduction and genetics Dordrecht [u.a.] : Springer Science + Business Media B.V., 1984 36(2018), 3 vom: 23. Nov., Seite 543-556 (DE-627)320573060 (DE-600)2016722-2 1573-7330 nnns volume:36 year:2018 number:3 day:23 month:11 pages:543-556 https://dx.doi.org/10.1007/s10815-018-1371-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 44.92 ASE AR 36 2018 3 23 11 543-556 |
| allfields_unstemmed |
10.1007/s10815-018-1371-6 doi (DE-627)SPR013540289 (SPR)s10815-018-1371-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.92 bkl Grady, Sicilia T. verfasserin aut Effect of intra-ovarian injection of mesenchymal stem cells in aged mares 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose This study aims to determine if intra-ovarian injection of bone marrow–derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Results Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries. Conclusions Intra-ovarian injection of bone marrow–derived MSCs altered gene expression but did not improve ovarian function in aged mares. Aging (dpeaa)DE-He213 Anti-Müllerian hormone (dpeaa)DE-He213 Equids (dpeaa)DE-He213 Fertility (dpeaa)DE-He213 Follicle-stimulating hormone (dpeaa)DE-He213 Follicular development (dpeaa)DE-He213 Oocyte (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Stem cells (dpeaa)DE-He213 Watts, Ashlee E. verfasserin aut Thompson, James A. verfasserin aut Penedo, M. Cecilia T. verfasserin aut Konganti, Kranti verfasserin aut Hinrichs, Katrin verfasserin aut Enthalten in Journal of assisted reproduction and genetics Dordrecht [u.a.] : Springer Science + Business Media B.V., 1984 36(2018), 3 vom: 23. Nov., Seite 543-556 (DE-627)320573060 (DE-600)2016722-2 1573-7330 nnns volume:36 year:2018 number:3 day:23 month:11 pages:543-556 https://dx.doi.org/10.1007/s10815-018-1371-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 44.92 ASE AR 36 2018 3 23 11 543-556 |
| allfieldsGer |
10.1007/s10815-018-1371-6 doi (DE-627)SPR013540289 (SPR)s10815-018-1371-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.92 bkl Grady, Sicilia T. verfasserin aut Effect of intra-ovarian injection of mesenchymal stem cells in aged mares 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose This study aims to determine if intra-ovarian injection of bone marrow–derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Results Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries. Conclusions Intra-ovarian injection of bone marrow–derived MSCs altered gene expression but did not improve ovarian function in aged mares. Aging (dpeaa)DE-He213 Anti-Müllerian hormone (dpeaa)DE-He213 Equids (dpeaa)DE-He213 Fertility (dpeaa)DE-He213 Follicle-stimulating hormone (dpeaa)DE-He213 Follicular development (dpeaa)DE-He213 Oocyte (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Stem cells (dpeaa)DE-He213 Watts, Ashlee E. verfasserin aut Thompson, James A. verfasserin aut Penedo, M. Cecilia T. verfasserin aut Konganti, Kranti verfasserin aut Hinrichs, Katrin verfasserin aut Enthalten in Journal of assisted reproduction and genetics Dordrecht [u.a.] : Springer Science + Business Media B.V., 1984 36(2018), 3 vom: 23. Nov., Seite 543-556 (DE-627)320573060 (DE-600)2016722-2 1573-7330 nnns volume:36 year:2018 number:3 day:23 month:11 pages:543-556 https://dx.doi.org/10.1007/s10815-018-1371-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 44.92 ASE AR 36 2018 3 23 11 543-556 |
| allfieldsSound |
10.1007/s10815-018-1371-6 doi (DE-627)SPR013540289 (SPR)s10815-018-1371-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.92 bkl Grady, Sicilia T. verfasserin aut Effect of intra-ovarian injection of mesenchymal stem cells in aged mares 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose This study aims to determine if intra-ovarian injection of bone marrow–derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Results Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries. Conclusions Intra-ovarian injection of bone marrow–derived MSCs altered gene expression but did not improve ovarian function in aged mares. Aging (dpeaa)DE-He213 Anti-Müllerian hormone (dpeaa)DE-He213 Equids (dpeaa)DE-He213 Fertility (dpeaa)DE-He213 Follicle-stimulating hormone (dpeaa)DE-He213 Follicular development (dpeaa)DE-He213 Oocyte (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Stem cells (dpeaa)DE-He213 Watts, Ashlee E. verfasserin aut Thompson, James A. verfasserin aut Penedo, M. Cecilia T. verfasserin aut Konganti, Kranti verfasserin aut Hinrichs, Katrin verfasserin aut Enthalten in Journal of assisted reproduction and genetics Dordrecht [u.a.] : Springer Science + Business Media B.V., 1984 36(2018), 3 vom: 23. Nov., Seite 543-556 (DE-627)320573060 (DE-600)2016722-2 1573-7330 nnns volume:36 year:2018 number:3 day:23 month:11 pages:543-556 https://dx.doi.org/10.1007/s10815-018-1371-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 44.92 ASE AR 36 2018 3 23 11 543-556 |
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English |
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Enthalten in Journal of assisted reproduction and genetics 36(2018), 3 vom: 23. Nov., Seite 543-556 volume:36 year:2018 number:3 day:23 month:11 pages:543-556 |
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Enthalten in Journal of assisted reproduction and genetics 36(2018), 3 vom: 23. Nov., Seite 543-556 volume:36 year:2018 number:3 day:23 month:11 pages:543-556 |
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Aging Anti-Müllerian hormone Equids Fertility Follicle-stimulating hormone Follicular development Oocyte Ovary Stem cells |
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Grady, Sicilia T. @@aut@@ Watts, Ashlee E. @@aut@@ Thompson, James A. @@aut@@ Penedo, M. Cecilia T. @@aut@@ Konganti, Kranti @@aut@@ Hinrichs, Katrin @@aut@@ |
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2018-11-23T00:00:00Z |
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Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Results Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries. 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Cecilia T.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Konganti, Kranti</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hinrichs, Katrin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of assisted reproduction and genetics</subfield><subfield code="d">Dordrecht [u.a.] : Springer Science + Business Media B.V., 1984</subfield><subfield code="g">36(2018), 3 vom: 23. 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Grady, Sicilia T. |
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Grady, Sicilia T. ddc 610 bkl 44.92 misc Aging misc Anti-Müllerian hormone misc Equids misc Fertility misc Follicle-stimulating hormone misc Follicular development misc Oocyte misc Ovary misc Stem cells Effect of intra-ovarian injection of mesenchymal stem cells in aged mares |
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610 ASE 44.92 bkl Effect of intra-ovarian injection of mesenchymal stem cells in aged mares Aging (dpeaa)DE-He213 Anti-Müllerian hormone (dpeaa)DE-He213 Equids (dpeaa)DE-He213 Fertility (dpeaa)DE-He213 Follicle-stimulating hormone (dpeaa)DE-He213 Follicular development (dpeaa)DE-He213 Oocyte (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Stem cells (dpeaa)DE-He213 |
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ddc 610 bkl 44.92 misc Aging misc Anti-Müllerian hormone misc Equids misc Fertility misc Follicle-stimulating hormone misc Follicular development misc Oocyte misc Ovary misc Stem cells |
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ddc 610 bkl 44.92 misc Aging misc Anti-Müllerian hormone misc Equids misc Fertility misc Follicle-stimulating hormone misc Follicular development misc Oocyte misc Ovary misc Stem cells |
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Effect of intra-ovarian injection of mesenchymal stem cells in aged mares |
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Effect of intra-ovarian injection of mesenchymal stem cells in aged mares |
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Journal of assisted reproduction and genetics |
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Journal of assisted reproduction and genetics |
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Grady, Sicilia T. Watts, Ashlee E. Thompson, James A. Penedo, M. Cecilia T. Konganti, Kranti Hinrichs, Katrin |
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Grady, Sicilia T. |
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effect of intra-ovarian injection of mesenchymal stem cells in aged mares |
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Effect of intra-ovarian injection of mesenchymal stem cells in aged mares |
| abstract |
Purpose This study aims to determine if intra-ovarian injection of bone marrow–derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Results Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries. Conclusions Intra-ovarian injection of bone marrow–derived MSCs altered gene expression but did not improve ovarian function in aged mares. |
| abstractGer |
Purpose This study aims to determine if intra-ovarian injection of bone marrow–derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Results Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries. Conclusions Intra-ovarian injection of bone marrow–derived MSCs altered gene expression but did not improve ovarian function in aged mares. |
| abstract_unstemmed |
Purpose This study aims to determine if intra-ovarian injection of bone marrow–derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. Methods Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing. Results Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries. Conclusions Intra-ovarian injection of bone marrow–derived MSCs altered gene expression but did not improve ovarian function in aged mares. |
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Effect of intra-ovarian injection of mesenchymal stem cells in aged mares |
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https://dx.doi.org/10.1007/s10815-018-1371-6 |
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Watts, Ashlee E. Thompson, James A. Penedo, M. Cecilia T. Konganti, Kranti Hinrichs, Katrin |
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| score |
7.400317 |