Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation
Abstract Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yang, Shu [verfasserIn] Zhang, Xin [verfasserIn] Zhao, Wentao [verfasserIn] Sun, Liquan [verfasserIn] Luo, Aiqin [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of materials science - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1966, 51(2015), 2 vom: 18. Sept., Seite 937-949 |
|---|---|
| Übergeordnetes Werk: |
volume:51 ; year:2015 ; number:2 ; day:18 ; month:09 ; pages:937-949 |
| Links: |
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| DOI / URN: |
10.1007/s10853-015-9423-0 |
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| Katalog-ID: |
SPR013913689 |
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| 520 | |a Abstract Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe%$_{3}%$O%$_{4}%$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe%$_{3}%$O%$_{4}%$ complex incorporated. Then Fe%$_{3}%$O%$_{4}%$–MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe%$_{3}%$O%$_{4}%$–MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. This report provides a possibility of the selective separation of protein in complex matrix. | ||
| 650 | 4 | |a Imprint Polymer |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Bulk Polymerization |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Binding Kinetic |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Binding Isotherm |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Template Protein |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Zhang, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Wentao |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Liquan |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Aiqin |e verfasserin |4 aut | |
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10.1007/s10853-015-9423-0 doi (DE-627)SPR013913689 (SPR)s10853-015-9423-0-e DE-627 ger DE-627 rakwb eng 670 ASE 51.00 bkl Yang, Shu verfasserin aut Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe%$_{3}%$O%$_{4}%$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe%$_{3}%$O%$_{4}%$ complex incorporated. Then Fe%$_{3}%$O%$_{4}%$–MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe%$_{3}%$O%$_{4}%$–MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. This report provides a possibility of the selective separation of protein in complex matrix. Imprint Polymer (dpeaa)DE-He213 Bulk Polymerization (dpeaa)DE-He213 Binding Kinetic (dpeaa)DE-He213 Binding Isotherm (dpeaa)DE-He213 Template Protein (dpeaa)DE-He213 Zhang, Xin verfasserin aut Zhao, Wentao verfasserin aut Sun, Liquan verfasserin aut Luo, Aiqin verfasserin aut Enthalten in Journal of materials science Dordrecht [u.a.] : Springer Science + Business Media B.V, 1966 51(2015), 2 vom: 18. Sept., Seite 937-949 (DE-627)315293969 (DE-600)2015305-3 1573-4803 nnns volume:51 year:2015 number:2 day:18 month:09 pages:937-949 https://dx.doi.org/10.1007/s10853-015-9423-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 51.00 ASE AR 51 2015 2 18 09 937-949 |
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10.1007/s10853-015-9423-0 doi (DE-627)SPR013913689 (SPR)s10853-015-9423-0-e DE-627 ger DE-627 rakwb eng 670 ASE 51.00 bkl Yang, Shu verfasserin aut Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe%$_{3}%$O%$_{4}%$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe%$_{3}%$O%$_{4}%$ complex incorporated. Then Fe%$_{3}%$O%$_{4}%$–MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe%$_{3}%$O%$_{4}%$–MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. This report provides a possibility of the selective separation of protein in complex matrix. Imprint Polymer (dpeaa)DE-He213 Bulk Polymerization (dpeaa)DE-He213 Binding Kinetic (dpeaa)DE-He213 Binding Isotherm (dpeaa)DE-He213 Template Protein (dpeaa)DE-He213 Zhang, Xin verfasserin aut Zhao, Wentao verfasserin aut Sun, Liquan verfasserin aut Luo, Aiqin verfasserin aut Enthalten in Journal of materials science Dordrecht [u.a.] : Springer Science + Business Media B.V, 1966 51(2015), 2 vom: 18. Sept., Seite 937-949 (DE-627)315293969 (DE-600)2015305-3 1573-4803 nnns volume:51 year:2015 number:2 day:18 month:09 pages:937-949 https://dx.doi.org/10.1007/s10853-015-9423-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 51.00 ASE AR 51 2015 2 18 09 937-949 |
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10.1007/s10853-015-9423-0 doi (DE-627)SPR013913689 (SPR)s10853-015-9423-0-e DE-627 ger DE-627 rakwb eng 670 ASE 51.00 bkl Yang, Shu verfasserin aut Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe%$_{3}%$O%$_{4}%$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe%$_{3}%$O%$_{4}%$ complex incorporated. Then Fe%$_{3}%$O%$_{4}%$–MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe%$_{3}%$O%$_{4}%$–MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. This report provides a possibility of the selective separation of protein in complex matrix. Imprint Polymer (dpeaa)DE-He213 Bulk Polymerization (dpeaa)DE-He213 Binding Kinetic (dpeaa)DE-He213 Binding Isotherm (dpeaa)DE-He213 Template Protein (dpeaa)DE-He213 Zhang, Xin verfasserin aut Zhao, Wentao verfasserin aut Sun, Liquan verfasserin aut Luo, Aiqin verfasserin aut Enthalten in Journal of materials science Dordrecht [u.a.] : Springer Science + Business Media B.V, 1966 51(2015), 2 vom: 18. Sept., Seite 937-949 (DE-627)315293969 (DE-600)2015305-3 1573-4803 nnns volume:51 year:2015 number:2 day:18 month:09 pages:937-949 https://dx.doi.org/10.1007/s10853-015-9423-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 51.00 ASE AR 51 2015 2 18 09 937-949 |
| allfieldsGer |
10.1007/s10853-015-9423-0 doi (DE-627)SPR013913689 (SPR)s10853-015-9423-0-e DE-627 ger DE-627 rakwb eng 670 ASE 51.00 bkl Yang, Shu verfasserin aut Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe%$_{3}%$O%$_{4}%$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe%$_{3}%$O%$_{4}%$ complex incorporated. Then Fe%$_{3}%$O%$_{4}%$–MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe%$_{3}%$O%$_{4}%$–MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. This report provides a possibility of the selective separation of protein in complex matrix. Imprint Polymer (dpeaa)DE-He213 Bulk Polymerization (dpeaa)DE-He213 Binding Kinetic (dpeaa)DE-He213 Binding Isotherm (dpeaa)DE-He213 Template Protein (dpeaa)DE-He213 Zhang, Xin verfasserin aut Zhao, Wentao verfasserin aut Sun, Liquan verfasserin aut Luo, Aiqin verfasserin aut Enthalten in Journal of materials science Dordrecht [u.a.] : Springer Science + Business Media B.V, 1966 51(2015), 2 vom: 18. Sept., Seite 937-949 (DE-627)315293969 (DE-600)2015305-3 1573-4803 nnns volume:51 year:2015 number:2 day:18 month:09 pages:937-949 https://dx.doi.org/10.1007/s10853-015-9423-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 51.00 ASE AR 51 2015 2 18 09 937-949 |
| allfieldsSound |
10.1007/s10853-015-9423-0 doi (DE-627)SPR013913689 (SPR)s10853-015-9423-0-e DE-627 ger DE-627 rakwb eng 670 ASE 51.00 bkl Yang, Shu verfasserin aut Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe%$_{3}%$O%$_{4}%$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe%$_{3}%$O%$_{4}%$ complex incorporated. Then Fe%$_{3}%$O%$_{4}%$–MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe%$_{3}%$O%$_{4}%$–MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. This report provides a possibility of the selective separation of protein in complex matrix. Imprint Polymer (dpeaa)DE-He213 Bulk Polymerization (dpeaa)DE-He213 Binding Kinetic (dpeaa)DE-He213 Binding Isotherm (dpeaa)DE-He213 Template Protein (dpeaa)DE-He213 Zhang, Xin verfasserin aut Zhao, Wentao verfasserin aut Sun, Liquan verfasserin aut Luo, Aiqin verfasserin aut Enthalten in Journal of materials science Dordrecht [u.a.] : Springer Science + Business Media B.V, 1966 51(2015), 2 vom: 18. Sept., Seite 937-949 (DE-627)315293969 (DE-600)2015305-3 1573-4803 nnns volume:51 year:2015 number:2 day:18 month:09 pages:937-949 https://dx.doi.org/10.1007/s10853-015-9423-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 51.00 ASE AR 51 2015 2 18 09 937-949 |
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Enthalten in Journal of materials science 51(2015), 2 vom: 18. Sept., Seite 937-949 volume:51 year:2015 number:2 day:18 month:09 pages:937-949 |
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Enthalten in Journal of materials science 51(2015), 2 vom: 18. Sept., Seite 937-949 volume:51 year:2015 number:2 day:18 month:09 pages:937-949 |
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Imprint Polymer Bulk Polymerization Binding Kinetic Binding Isotherm Template Protein |
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Yang, Shu @@aut@@ Zhang, Xin @@aut@@ Zhao, Wentao @@aut@@ Sun, Liquan @@aut@@ Luo, Aiqin @@aut@@ |
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2015-09-18T00:00:00Z |
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In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe%$_{3}%$O%$_{4}%$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe%$_{3}%$O%$_{4}%$ complex incorporated. Then Fe%$_{3}%$O%$_{4}%$–MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe%$_{3}%$O%$_{4}%$–MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. 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Sept., Seite 937-949</subfield><subfield code="w">(DE-627)315293969</subfield><subfield code="w">(DE-600)2015305-3</subfield><subfield code="x">1573-4803</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:51</subfield><subfield code="g">year:2015</subfield><subfield code="g">number:2</subfield><subfield code="g">day:18</subfield><subfield code="g">month:09</subfield><subfield code="g">pages:937-949</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s10853-015-9423-0</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" 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Yang, Shu |
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Yang, Shu ddc 670 bkl 51.00 misc Imprint Polymer misc Bulk Polymerization misc Binding Kinetic misc Binding Isotherm misc Template Protein Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation |
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670 ASE 51.00 bkl Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation Imprint Polymer (dpeaa)DE-He213 Bulk Polymerization (dpeaa)DE-He213 Binding Kinetic (dpeaa)DE-He213 Binding Isotherm (dpeaa)DE-He213 Template Protein (dpeaa)DE-He213 |
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Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation |
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Yang, Shu Zhang, Xin Zhao, Wentao Sun, Liquan Luo, Aiqin |
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preparation and evaluation of fe%$_{3}%$o%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation |
| title_auth |
Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation |
| abstract |
Abstract Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe%$_{3}%$O%$_{4}%$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe%$_{3}%$O%$_{4}%$ complex incorporated. Then Fe%$_{3}%$O%$_{4}%$–MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe%$_{3}%$O%$_{4}%$–MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. This report provides a possibility of the selective separation of protein in complex matrix. |
| abstractGer |
Abstract Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe%$_{3}%$O%$_{4}%$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe%$_{3}%$O%$_{4}%$ complex incorporated. Then Fe%$_{3}%$O%$_{4}%$–MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe%$_{3}%$O%$_{4}%$–MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. This report provides a possibility of the selective separation of protein in complex matrix. |
| abstract_unstemmed |
Abstract Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe%$_{3}%$O%$_{4}%$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe%$_{3}%$O%$_{4}%$ complex incorporated. Then Fe%$_{3}%$O%$_{4}%$–MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe%$_{3}%$O%$_{4}%$–MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe%$_{3}%$O%$_{4}%$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. This report provides a possibility of the selective separation of protein in complex matrix. |
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2 |
| title_short |
Preparation and evaluation of Fe%$_{3}%$O%$_{4}%$ nanoparticles incorporated molecularly imprinted polymers for protein separation |
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https://dx.doi.org/10.1007/s10853-015-9423-0 |
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Zhang, Xin Zhao, Wentao Sun, Liquan Luo, Aiqin |
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| doi_str |
10.1007/s10853-015-9423-0 |
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2024-07-03T22:58:46.450Z |
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| score |
7.402316 |