Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein
Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of...
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| Autor*in: |
Tyan, Yu-Chang [verfasserIn] Yang, Ming-Hui [verfasserIn] Chung, Tze-Wen [verfasserIn] Chen, Wen-Cheng [verfasserIn] Wang, Ming-Chen [verfasserIn] Chen, Yi-Ling [verfasserIn] Huang, Shu-Ling [verfasserIn] Huang, Ying-Fong [verfasserIn] Jong, Shiang-Bin [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2011 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of materials science - Dordrecht : Springer Science + Business Media B.V, 1990, 22(2011), 6 vom: 09. Apr., Seite 1383-1391 |
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| Übergeordnetes Werk: |
volume:22 ; year:2011 ; number:6 ; day:09 ; month:04 ; pages:1383-1391 |
| Links: |
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| DOI / URN: |
10.1007/s10856-011-4312-9 |
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| Katalog-ID: |
SPR014123096 |
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| 245 | 1 | 0 | |a Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein |
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| 520 | |a Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of the attachment of antibody using alkanethiols, to form SAMs on the face of Au-quartz crystal microbalance (QCM) surfaces. In this study, the attachment of anti-α-fetoprotein monoclonal antibody to a SAMs surface of 11-mercaptoundecanoic acid was achieved using water-soluble N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as coupling agents. Surface analyses were utilized by X-ray photoelectron spectroscopy and atomic force microscopy. The quantization of immobilized antibody was characterized by the frequency shift of QCM and the radioactivity change of 125I labeled antibody. The limit of detection and linear range of the calibration curve of the QCM method were 15 ng/ml and 15–850 ng/ml. The correlation coefficients of α-fetoprotein concentration between QCM and radioimmunoassay were 0.9903 and 0.9750 for the standards and serum samples, respectively. This report illustrates an investigation of SAMs for the preparation of covalently immobilized antibody biosensors. | ||
| 650 | 4 | |a Quartz Crystal Microbalance |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Quartz Crystal Microbalance Sensor |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Sauerbrey Equation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a SAMs Surface |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Quartz Crystal Microbalance Measurement |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Yang, Ming-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Chung, Tze-Wen |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Wen-Cheng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ming-Chen |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yi-Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Shu-Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Ying-Fong |e verfasserin |4 aut | |
| 700 | 1 | |a Jong, Shiang-Bin |e verfasserin |4 aut | |
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| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
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| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
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| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
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| 912 | |a GBV_ILN_2122 | ||
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| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
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| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
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10.1007/s10856-011-4312-9 doi (DE-627)SPR014123096 (SPR)s10856-011-4312-9-e DE-627 ger DE-627 rakwb eng 610 670 ASE 44.09 bkl 51.40 bkl Tyan, Yu-Chang verfasserin aut Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of the attachment of antibody using alkanethiols, to form SAMs on the face of Au-quartz crystal microbalance (QCM) surfaces. In this study, the attachment of anti-α-fetoprotein monoclonal antibody to a SAMs surface of 11-mercaptoundecanoic acid was achieved using water-soluble N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as coupling agents. Surface analyses were utilized by X-ray photoelectron spectroscopy and atomic force microscopy. The quantization of immobilized antibody was characterized by the frequency shift of QCM and the radioactivity change of 125I labeled antibody. The limit of detection and linear range of the calibration curve of the QCM method were 15 ng/ml and 15–850 ng/ml. The correlation coefficients of α-fetoprotein concentration between QCM and radioimmunoassay were 0.9903 and 0.9750 for the standards and serum samples, respectively. This report illustrates an investigation of SAMs for the preparation of covalently immobilized antibody biosensors. Quartz Crystal Microbalance (dpeaa)DE-He213 Quartz Crystal Microbalance Sensor (dpeaa)DE-He213 Sauerbrey Equation (dpeaa)DE-He213 SAMs Surface (dpeaa)DE-He213 Quartz Crystal Microbalance Measurement (dpeaa)DE-He213 Yang, Ming-Hui verfasserin aut Chung, Tze-Wen verfasserin aut Chen, Wen-Cheng verfasserin aut Wang, Ming-Chen verfasserin aut Chen, Yi-Ling verfasserin aut Huang, Shu-Ling verfasserin aut Huang, Ying-Fong verfasserin aut Jong, Shiang-Bin verfasserin aut Enthalten in Journal of materials science Dordrecht : Springer Science + Business Media B.V, 1990 22(2011), 6 vom: 09. Apr., Seite 1383-1391 (DE-627)317827316 (DE-600)2016995-4 1573-4838 nnns volume:22 year:2011 number:6 day:09 month:04 pages:1383-1391 https://dx.doi.org/10.1007/s10856-011-4312-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.09 ASE 51.40 ASE AR 22 2011 6 09 04 1383-1391 |
| spelling |
10.1007/s10856-011-4312-9 doi (DE-627)SPR014123096 (SPR)s10856-011-4312-9-e DE-627 ger DE-627 rakwb eng 610 670 ASE 44.09 bkl 51.40 bkl Tyan, Yu-Chang verfasserin aut Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of the attachment of antibody using alkanethiols, to form SAMs on the face of Au-quartz crystal microbalance (QCM) surfaces. In this study, the attachment of anti-α-fetoprotein monoclonal antibody to a SAMs surface of 11-mercaptoundecanoic acid was achieved using water-soluble N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as coupling agents. Surface analyses were utilized by X-ray photoelectron spectroscopy and atomic force microscopy. The quantization of immobilized antibody was characterized by the frequency shift of QCM and the radioactivity change of 125I labeled antibody. The limit of detection and linear range of the calibration curve of the QCM method were 15 ng/ml and 15–850 ng/ml. The correlation coefficients of α-fetoprotein concentration between QCM and radioimmunoassay were 0.9903 and 0.9750 for the standards and serum samples, respectively. This report illustrates an investigation of SAMs for the preparation of covalently immobilized antibody biosensors. Quartz Crystal Microbalance (dpeaa)DE-He213 Quartz Crystal Microbalance Sensor (dpeaa)DE-He213 Sauerbrey Equation (dpeaa)DE-He213 SAMs Surface (dpeaa)DE-He213 Quartz Crystal Microbalance Measurement (dpeaa)DE-He213 Yang, Ming-Hui verfasserin aut Chung, Tze-Wen verfasserin aut Chen, Wen-Cheng verfasserin aut Wang, Ming-Chen verfasserin aut Chen, Yi-Ling verfasserin aut Huang, Shu-Ling verfasserin aut Huang, Ying-Fong verfasserin aut Jong, Shiang-Bin verfasserin aut Enthalten in Journal of materials science Dordrecht : Springer Science + Business Media B.V, 1990 22(2011), 6 vom: 09. Apr., Seite 1383-1391 (DE-627)317827316 (DE-600)2016995-4 1573-4838 nnns volume:22 year:2011 number:6 day:09 month:04 pages:1383-1391 https://dx.doi.org/10.1007/s10856-011-4312-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.09 ASE 51.40 ASE AR 22 2011 6 09 04 1383-1391 |
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10.1007/s10856-011-4312-9 doi (DE-627)SPR014123096 (SPR)s10856-011-4312-9-e DE-627 ger DE-627 rakwb eng 610 670 ASE 44.09 bkl 51.40 bkl Tyan, Yu-Chang verfasserin aut Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of the attachment of antibody using alkanethiols, to form SAMs on the face of Au-quartz crystal microbalance (QCM) surfaces. In this study, the attachment of anti-α-fetoprotein monoclonal antibody to a SAMs surface of 11-mercaptoundecanoic acid was achieved using water-soluble N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as coupling agents. Surface analyses were utilized by X-ray photoelectron spectroscopy and atomic force microscopy. The quantization of immobilized antibody was characterized by the frequency shift of QCM and the radioactivity change of 125I labeled antibody. The limit of detection and linear range of the calibration curve of the QCM method were 15 ng/ml and 15–850 ng/ml. The correlation coefficients of α-fetoprotein concentration between QCM and radioimmunoassay were 0.9903 and 0.9750 for the standards and serum samples, respectively. This report illustrates an investigation of SAMs for the preparation of covalently immobilized antibody biosensors. Quartz Crystal Microbalance (dpeaa)DE-He213 Quartz Crystal Microbalance Sensor (dpeaa)DE-He213 Sauerbrey Equation (dpeaa)DE-He213 SAMs Surface (dpeaa)DE-He213 Quartz Crystal Microbalance Measurement (dpeaa)DE-He213 Yang, Ming-Hui verfasserin aut Chung, Tze-Wen verfasserin aut Chen, Wen-Cheng verfasserin aut Wang, Ming-Chen verfasserin aut Chen, Yi-Ling verfasserin aut Huang, Shu-Ling verfasserin aut Huang, Ying-Fong verfasserin aut Jong, Shiang-Bin verfasserin aut Enthalten in Journal of materials science Dordrecht : Springer Science + Business Media B.V, 1990 22(2011), 6 vom: 09. Apr., Seite 1383-1391 (DE-627)317827316 (DE-600)2016995-4 1573-4838 nnns volume:22 year:2011 number:6 day:09 month:04 pages:1383-1391 https://dx.doi.org/10.1007/s10856-011-4312-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.09 ASE 51.40 ASE AR 22 2011 6 09 04 1383-1391 |
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10.1007/s10856-011-4312-9 doi (DE-627)SPR014123096 (SPR)s10856-011-4312-9-e DE-627 ger DE-627 rakwb eng 610 670 ASE 44.09 bkl 51.40 bkl Tyan, Yu-Chang verfasserin aut Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of the attachment of antibody using alkanethiols, to form SAMs on the face of Au-quartz crystal microbalance (QCM) surfaces. In this study, the attachment of anti-α-fetoprotein monoclonal antibody to a SAMs surface of 11-mercaptoundecanoic acid was achieved using water-soluble N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as coupling agents. Surface analyses were utilized by X-ray photoelectron spectroscopy and atomic force microscopy. The quantization of immobilized antibody was characterized by the frequency shift of QCM and the radioactivity change of 125I labeled antibody. The limit of detection and linear range of the calibration curve of the QCM method were 15 ng/ml and 15–850 ng/ml. The correlation coefficients of α-fetoprotein concentration between QCM and radioimmunoassay were 0.9903 and 0.9750 for the standards and serum samples, respectively. This report illustrates an investigation of SAMs for the preparation of covalently immobilized antibody biosensors. Quartz Crystal Microbalance (dpeaa)DE-He213 Quartz Crystal Microbalance Sensor (dpeaa)DE-He213 Sauerbrey Equation (dpeaa)DE-He213 SAMs Surface (dpeaa)DE-He213 Quartz Crystal Microbalance Measurement (dpeaa)DE-He213 Yang, Ming-Hui verfasserin aut Chung, Tze-Wen verfasserin aut Chen, Wen-Cheng verfasserin aut Wang, Ming-Chen verfasserin aut Chen, Yi-Ling verfasserin aut Huang, Shu-Ling verfasserin aut Huang, Ying-Fong verfasserin aut Jong, Shiang-Bin verfasserin aut Enthalten in Journal of materials science Dordrecht : Springer Science + Business Media B.V, 1990 22(2011), 6 vom: 09. Apr., Seite 1383-1391 (DE-627)317827316 (DE-600)2016995-4 1573-4838 nnns volume:22 year:2011 number:6 day:09 month:04 pages:1383-1391 https://dx.doi.org/10.1007/s10856-011-4312-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.09 ASE 51.40 ASE AR 22 2011 6 09 04 1383-1391 |
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10.1007/s10856-011-4312-9 doi (DE-627)SPR014123096 (SPR)s10856-011-4312-9-e DE-627 ger DE-627 rakwb eng 610 670 ASE 44.09 bkl 51.40 bkl Tyan, Yu-Chang verfasserin aut Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of the attachment of antibody using alkanethiols, to form SAMs on the face of Au-quartz crystal microbalance (QCM) surfaces. In this study, the attachment of anti-α-fetoprotein monoclonal antibody to a SAMs surface of 11-mercaptoundecanoic acid was achieved using water-soluble N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as coupling agents. Surface analyses were utilized by X-ray photoelectron spectroscopy and atomic force microscopy. The quantization of immobilized antibody was characterized by the frequency shift of QCM and the radioactivity change of 125I labeled antibody. The limit of detection and linear range of the calibration curve of the QCM method were 15 ng/ml and 15–850 ng/ml. The correlation coefficients of α-fetoprotein concentration between QCM and radioimmunoassay were 0.9903 and 0.9750 for the standards and serum samples, respectively. This report illustrates an investigation of SAMs for the preparation of covalently immobilized antibody biosensors. Quartz Crystal Microbalance (dpeaa)DE-He213 Quartz Crystal Microbalance Sensor (dpeaa)DE-He213 Sauerbrey Equation (dpeaa)DE-He213 SAMs Surface (dpeaa)DE-He213 Quartz Crystal Microbalance Measurement (dpeaa)DE-He213 Yang, Ming-Hui verfasserin aut Chung, Tze-Wen verfasserin aut Chen, Wen-Cheng verfasserin aut Wang, Ming-Chen verfasserin aut Chen, Yi-Ling verfasserin aut Huang, Shu-Ling verfasserin aut Huang, Ying-Fong verfasserin aut Jong, Shiang-Bin verfasserin aut Enthalten in Journal of materials science Dordrecht : Springer Science + Business Media B.V, 1990 22(2011), 6 vom: 09. Apr., Seite 1383-1391 (DE-627)317827316 (DE-600)2016995-4 1573-4838 nnns volume:22 year:2011 number:6 day:09 month:04 pages:1383-1391 https://dx.doi.org/10.1007/s10856-011-4312-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.09 ASE 51.40 ASE AR 22 2011 6 09 04 1383-1391 |
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Enthalten in Journal of materials science 22(2011), 6 vom: 09. Apr., Seite 1383-1391 volume:22 year:2011 number:6 day:09 month:04 pages:1383-1391 |
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Quartz Crystal Microbalance Quartz Crystal Microbalance Sensor Sauerbrey Equation SAMs Surface Quartz Crystal Microbalance Measurement |
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Tyan, Yu-Chang @@aut@@ Yang, Ming-Hui @@aut@@ Chung, Tze-Wen @@aut@@ Chen, Wen-Cheng @@aut@@ Wang, Ming-Chen @@aut@@ Chen, Yi-Ling @@aut@@ Huang, Shu-Ling @@aut@@ Huang, Ying-Fong @@aut@@ Jong, Shiang-Bin @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR014123096</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519163532.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201006s2011 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s10856-011-4312-9</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR014123096</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s10856-011-4312-9-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="a">670</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.09</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">51.40</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Tyan, Yu-Chang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2011</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of the attachment of antibody using alkanethiols, to form SAMs on the face of Au-quartz crystal microbalance (QCM) surfaces. In this study, the attachment of anti-α-fetoprotein monoclonal antibody to a SAMs surface of 11-mercaptoundecanoic acid was achieved using water-soluble N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as coupling agents. Surface analyses were utilized by X-ray photoelectron spectroscopy and atomic force microscopy. The quantization of immobilized antibody was characterized by the frequency shift of QCM and the radioactivity change of 125I labeled antibody. The limit of detection and linear range of the calibration curve of the QCM method were 15 ng/ml and 15–850 ng/ml. The correlation coefficients of α-fetoprotein concentration between QCM and radioimmunoassay were 0.9903 and 0.9750 for the standards and serum samples, respectively. 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|
| author |
Tyan, Yu-Chang |
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Tyan, Yu-Chang ddc 610 bkl 44.09 bkl 51.40 misc Quartz Crystal Microbalance misc Quartz Crystal Microbalance Sensor misc Sauerbrey Equation misc SAMs Surface misc Quartz Crystal Microbalance Measurement Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein |
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Tyan, Yu-Chang |
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610 670 ASE 44.09 bkl 51.40 bkl Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein Quartz Crystal Microbalance (dpeaa)DE-He213 Quartz Crystal Microbalance Sensor (dpeaa)DE-He213 Sauerbrey Equation (dpeaa)DE-He213 SAMs Surface (dpeaa)DE-He213 Quartz Crystal Microbalance Measurement (dpeaa)DE-He213 |
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ddc 610 bkl 44.09 bkl 51.40 misc Quartz Crystal Microbalance misc Quartz Crystal Microbalance Sensor misc Sauerbrey Equation misc SAMs Surface misc Quartz Crystal Microbalance Measurement |
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ddc 610 bkl 44.09 bkl 51.40 misc Quartz Crystal Microbalance misc Quartz Crystal Microbalance Sensor misc Sauerbrey Equation misc SAMs Surface misc Quartz Crystal Microbalance Measurement |
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ddc 610 bkl 44.09 bkl 51.40 misc Quartz Crystal Microbalance misc Quartz Crystal Microbalance Sensor misc Sauerbrey Equation misc SAMs Surface misc Quartz Crystal Microbalance Measurement |
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Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein |
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Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein |
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Tyan, Yu-Chang |
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Journal of materials science |
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Journal of materials science |
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eng |
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600 - Technology |
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Tyan, Yu-Chang Yang, Ming-Hui Chung, Tze-Wen Chen, Wen-Cheng Wang, Ming-Chen Chen, Yi-Ling Huang, Shu-Ling Huang, Ying-Fong Jong, Shiang-Bin |
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610 670 ASE 44.09 bkl 51.40 bkl |
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Elektronische Aufsätze |
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Tyan, Yu-Chang |
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10.1007/s10856-011-4312-9 |
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610 670 |
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verfasserin |
| title_sort |
characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein |
| title_auth |
Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein |
| abstract |
Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of the attachment of antibody using alkanethiols, to form SAMs on the face of Au-quartz crystal microbalance (QCM) surfaces. In this study, the attachment of anti-α-fetoprotein monoclonal antibody to a SAMs surface of 11-mercaptoundecanoic acid was achieved using water-soluble N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as coupling agents. Surface analyses were utilized by X-ray photoelectron spectroscopy and atomic force microscopy. The quantization of immobilized antibody was characterized by the frequency shift of QCM and the radioactivity change of 125I labeled antibody. The limit of detection and linear range of the calibration curve of the QCM method were 15 ng/ml and 15–850 ng/ml. The correlation coefficients of α-fetoprotein concentration between QCM and radioimmunoassay were 0.9903 and 0.9750 for the standards and serum samples, respectively. This report illustrates an investigation of SAMs for the preparation of covalently immobilized antibody biosensors. |
| abstractGer |
Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of the attachment of antibody using alkanethiols, to form SAMs on the face of Au-quartz crystal microbalance (QCM) surfaces. In this study, the attachment of anti-α-fetoprotein monoclonal antibody to a SAMs surface of 11-mercaptoundecanoic acid was achieved using water-soluble N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as coupling agents. Surface analyses were utilized by X-ray photoelectron spectroscopy and atomic force microscopy. The quantization of immobilized antibody was characterized by the frequency shift of QCM and the radioactivity change of 125I labeled antibody. The limit of detection and linear range of the calibration curve of the QCM method were 15 ng/ml and 15–850 ng/ml. The correlation coefficients of α-fetoprotein concentration between QCM and radioimmunoassay were 0.9903 and 0.9750 for the standards and serum samples, respectively. This report illustrates an investigation of SAMs for the preparation of covalently immobilized antibody biosensors. |
| abstract_unstemmed |
Abstract Self-assembled monolayers (SAMs) on coinage metallic material can provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently, a bio-sensing system has been produced by analysis of the attachment of antibody using alkanethiols, to form SAMs on the face of Au-quartz crystal microbalance (QCM) surfaces. In this study, the attachment of anti-α-fetoprotein monoclonal antibody to a SAMs surface of 11-mercaptoundecanoic acid was achieved using water-soluble N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide as coupling agents. Surface analyses were utilized by X-ray photoelectron spectroscopy and atomic force microscopy. The quantization of immobilized antibody was characterized by the frequency shift of QCM and the radioactivity change of 125I labeled antibody. The limit of detection and linear range of the calibration curve of the QCM method were 15 ng/ml and 15–850 ng/ml. The correlation coefficients of α-fetoprotein concentration between QCM and radioimmunoassay were 0.9903 and 0.9750 for the standards and serum samples, respectively. This report illustrates an investigation of SAMs for the preparation of covalently immobilized antibody biosensors. |
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6 |
| title_short |
Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein |
| url |
https://dx.doi.org/10.1007/s10856-011-4312-9 |
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Yang, Ming-Hui Chung, Tze-Wen Chen, Wen-Cheng Wang, Ming-Chen Chen, Yi-Ling Huang, Shu-Ling Huang, Ying-Fong Jong, Shiang-Bin |
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Yang, Ming-Hui Chung, Tze-Wen Chen, Wen-Cheng Wang, Ming-Chen Chen, Yi-Ling Huang, Shu-Ling Huang, Ying-Fong Jong, Shiang-Bin |
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2024-07-04T00:11:33.914Z |
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| score |
7.399828 |