The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae)

Abstract In this work, we analyzed the effects of coffee seed proteins, especially Cc-$ LTP_{1} $ on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, whic...
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Gespeichert in:

Autor*in:	Zottich, Umberto [verfasserIn] Da Cunha, Maura [verfasserIn] Dias, Germana B. [verfasserIn] Rabelo, Guilherme R. [verfasserIn] Oliveira, Antonia Elenir A. [verfasserIn] Carvalho, André O. [verfasserIn] Fernandes, Kátia Valevski S. [verfasserIn] do Nascimento, Viviane V. [verfasserIn] Gomes, Valdirene M. [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	Lipid transfer protein Defense protein

Übergeordnetes Werk:	Enthalten in: The protein journal - Dordrecht : Springer Science + Business Media B.V., 2004, 33(2014), 5 vom: 06. Aug., Seite 422-431
Übergeordnetes Werk:	volume:33 ; year:2014 ; number:5 ; day:06 ; month:08 ; pages:422-431

Links:	Volltext

DOI / URN:	10.1007/s10930-014-9575-9

Katalog-ID:	SPR014723077

Internformat


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100	1		\|a Zottich, Umberto \|e verfasserin \|4 aut
245	1	4	\|a The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae)
264		1	\|c 2014
336			\|a Text \|b txt \|2 rdacontent
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520			\|a Abstract In this work, we analyzed the effects of coffee seed proteins, especially Cc-$ LTP_{1} $ on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-$ LTP_{1} $, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-$ LTP_{1} $ was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-$ LTP_{1} $ has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-$ LTP_{1} $ has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues.
650		4	\|a Lipid transfer protein \|7 (dpeaa)DE-He213
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700	1		\|a Da Cunha, Maura \|e verfasserin \|4 aut
700	1		\|a Dias, Germana B. \|e verfasserin \|4 aut
700	1		\|a Rabelo, Guilherme R. \|e verfasserin \|4 aut
700	1		\|a Oliveira, Antonia Elenir A. \|e verfasserin \|4 aut
700	1		\|a Carvalho, André O. \|e verfasserin \|4 aut
700	1		\|a Fernandes, Kátia Valevski S. \|e verfasserin \|4 aut
700	1		\|a do Nascimento, Viviane V. \|e verfasserin \|4 aut
700	1		\|a Gomes, Valdirene M. \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t The protein journal \|d Dordrecht : Springer Science + Business Media B.V., 2004 \|g 33(2014), 5 vom: 06. Aug., Seite 422-431 \|w (DE-627)385615884 \|w (DE-600)2143071-8 \|x 1573-4943 \|7 nnns
773	1	8	\|g volume:33 \|g year:2014 \|g number:5 \|g day:06 \|g month:08 \|g pages:422-431
856	4	0	\|u https://dx.doi.org/10.1007/s10930-014-9575-9 \|z lizenzpflichtig \|3 Volltext
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912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
936	b	k	\|a 35.76 \|q ASE
951			\|a AR
952			\|d 33 \|j 2014 \|e 5 \|b 06 \|c 08 \|h 422-431

Indexfelder

author_variant	u z uz c m d cm cmd g b d gb gbd g r r gr grr a e a o aea aeao a o c ao aoc k v s f kvs kvsf n v v d nvv nvvd v m g vm vmg
matchkey_str	article:15734943:2014----::htxctoaiitasepoenct1rmofaaehrsesnhlradvlpetfalsb
hierarchy_sort_str	2014
bklnumber	35.76
publishDate	2014
allfields	10.1007/s10930-014-9575-9 doi (DE-627)SPR014723077 (SPR)s10930-014-9575-9-e DE-627 ger DE-627 rakwb eng 540 ASE 35.76 bkl Zottich, Umberto verfasserin aut The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae) 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In this work, we analyzed the effects of coffee seed proteins, especially Cc-$ LTP_{1} $ on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-$ LTP_{1} $, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-$ LTP_{1} $ was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-$ LTP_{1} $ has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-$ LTP_{1} $ has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues. Lipid transfer protein (dpeaa)DE-He213 Defense protein (dpeaa)DE-He213 Da Cunha, Maura verfasserin aut Dias, Germana B. verfasserin aut Rabelo, Guilherme R. verfasserin aut Oliveira, Antonia Elenir A. verfasserin aut Carvalho, André O. verfasserin aut Fernandes, Kátia Valevski S. verfasserin aut do Nascimento, Viviane V. verfasserin aut Gomes, Valdirene M. verfasserin aut Enthalten in The protein journal Dordrecht : Springer Science + Business Media B.V., 2004 33(2014), 5 vom: 06. Aug., Seite 422-431 (DE-627)385615884 (DE-600)2143071-8 1573-4943 nnns volume:33 year:2014 number:5 day:06 month:08 pages:422-431 https://dx.doi.org/10.1007/s10930-014-9575-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.76 ASE AR 33 2014 5 06 08 422-431
spelling	10.1007/s10930-014-9575-9 doi (DE-627)SPR014723077 (SPR)s10930-014-9575-9-e DE-627 ger DE-627 rakwb eng 540 ASE 35.76 bkl Zottich, Umberto verfasserin aut The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae) 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In this work, we analyzed the effects of coffee seed proteins, especially Cc-$ LTP_{1} $ on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-$ LTP_{1} $, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-$ LTP_{1} $ was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-$ LTP_{1} $ has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-$ LTP_{1} $ has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues. Lipid transfer protein (dpeaa)DE-He213 Defense protein (dpeaa)DE-He213 Da Cunha, Maura verfasserin aut Dias, Germana B. verfasserin aut Rabelo, Guilherme R. verfasserin aut Oliveira, Antonia Elenir A. verfasserin aut Carvalho, André O. verfasserin aut Fernandes, Kátia Valevski S. verfasserin aut do Nascimento, Viviane V. verfasserin aut Gomes, Valdirene M. verfasserin aut Enthalten in The protein journal Dordrecht : Springer Science + Business Media B.V., 2004 33(2014), 5 vom: 06. Aug., Seite 422-431 (DE-627)385615884 (DE-600)2143071-8 1573-4943 nnns volume:33 year:2014 number:5 day:06 month:08 pages:422-431 https://dx.doi.org/10.1007/s10930-014-9575-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.76 ASE AR 33 2014 5 06 08 422-431
allfields_unstemmed	10.1007/s10930-014-9575-9 doi (DE-627)SPR014723077 (SPR)s10930-014-9575-9-e DE-627 ger DE-627 rakwb eng 540 ASE 35.76 bkl Zottich, Umberto verfasserin aut The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae) 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In this work, we analyzed the effects of coffee seed proteins, especially Cc-$ LTP_{1} $ on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-$ LTP_{1} $, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-$ LTP_{1} $ was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-$ LTP_{1} $ has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-$ LTP_{1} $ has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues. Lipid transfer protein (dpeaa)DE-He213 Defense protein (dpeaa)DE-He213 Da Cunha, Maura verfasserin aut Dias, Germana B. verfasserin aut Rabelo, Guilherme R. verfasserin aut Oliveira, Antonia Elenir A. verfasserin aut Carvalho, André O. verfasserin aut Fernandes, Kátia Valevski S. verfasserin aut do Nascimento, Viviane V. verfasserin aut Gomes, Valdirene M. verfasserin aut Enthalten in The protein journal Dordrecht : Springer Science + Business Media B.V., 2004 33(2014), 5 vom: 06. Aug., Seite 422-431 (DE-627)385615884 (DE-600)2143071-8 1573-4943 nnns volume:33 year:2014 number:5 day:06 month:08 pages:422-431 https://dx.doi.org/10.1007/s10930-014-9575-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.76 ASE AR 33 2014 5 06 08 422-431
allfieldsGer	10.1007/s10930-014-9575-9 doi (DE-627)SPR014723077 (SPR)s10930-014-9575-9-e DE-627 ger DE-627 rakwb eng 540 ASE 35.76 bkl Zottich, Umberto verfasserin aut The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae) 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In this work, we analyzed the effects of coffee seed proteins, especially Cc-$ LTP_{1} $ on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-$ LTP_{1} $, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-$ LTP_{1} $ was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-$ LTP_{1} $ has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-$ LTP_{1} $ has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues. Lipid transfer protein (dpeaa)DE-He213 Defense protein (dpeaa)DE-He213 Da Cunha, Maura verfasserin aut Dias, Germana B. verfasserin aut Rabelo, Guilherme R. verfasserin aut Oliveira, Antonia Elenir A. verfasserin aut Carvalho, André O. verfasserin aut Fernandes, Kátia Valevski S. verfasserin aut do Nascimento, Viviane V. verfasserin aut Gomes, Valdirene M. verfasserin aut Enthalten in The protein journal Dordrecht : Springer Science + Business Media B.V., 2004 33(2014), 5 vom: 06. Aug., Seite 422-431 (DE-627)385615884 (DE-600)2143071-8 1573-4943 nnns volume:33 year:2014 number:5 day:06 month:08 pages:422-431 https://dx.doi.org/10.1007/s10930-014-9575-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.76 ASE AR 33 2014 5 06 08 422-431
allfieldsSound	10.1007/s10930-014-9575-9 doi (DE-627)SPR014723077 (SPR)s10930-014-9575-9-e DE-627 ger DE-627 rakwb eng 540 ASE 35.76 bkl Zottich, Umberto verfasserin aut The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae) 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In this work, we analyzed the effects of coffee seed proteins, especially Cc-$ LTP_{1} $ on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-$ LTP_{1} $, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-$ LTP_{1} $ was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-$ LTP_{1} $ has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-$ LTP_{1} $ has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues. Lipid transfer protein (dpeaa)DE-He213 Defense protein (dpeaa)DE-He213 Da Cunha, Maura verfasserin aut Dias, Germana B. verfasserin aut Rabelo, Guilherme R. verfasserin aut Oliveira, Antonia Elenir A. verfasserin aut Carvalho, André O. verfasserin aut Fernandes, Kátia Valevski S. verfasserin aut do Nascimento, Viviane V. verfasserin aut Gomes, Valdirene M. verfasserin aut Enthalten in The protein journal Dordrecht : Springer Science + Business Media B.V., 2004 33(2014), 5 vom: 06. Aug., Seite 422-431 (DE-627)385615884 (DE-600)2143071-8 1573-4943 nnns volume:33 year:2014 number:5 day:06 month:08 pages:422-431 https://dx.doi.org/10.1007/s10930-014-9575-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.76 ASE AR 33 2014 5 06 08 422-431
language	English
source	Enthalten in The protein journal 33(2014), 5 vom: 06. Aug., Seite 422-431 volume:33 year:2014 number:5 day:06 month:08 pages:422-431
sourceStr	Enthalten in The protein journal 33(2014), 5 vom: 06. Aug., Seite 422-431 volume:33 year:2014 number:5 day:06 month:08 pages:422-431
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Lipid transfer protein Defense protein
dewey-raw	540
isfreeaccess_bool	false
container_title	The protein journal
authorswithroles_txt_mv	Zottich, Umberto @@aut@@ Da Cunha, Maura @@aut@@ Dias, Germana B. @@aut@@ Rabelo, Guilherme R. @@aut@@ Oliveira, Antonia Elenir A. @@aut@@ Carvalho, André O. @@aut@@ Fernandes, Kátia Valevski S. @@aut@@ do Nascimento, Viviane V. @@aut@@ Gomes, Valdirene M. @@aut@@
publishDateDaySort_date	2014-08-06T00:00:00Z
hierarchy_top_id	385615884
dewey-sort	3540
id	SPR014723077
language_de	englisch
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author	Zottich, Umberto
spellingShingle	Zottich, Umberto ddc 540 bkl 35.76 misc Lipid transfer protein misc Defense protein The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae)
authorStr	Zottich, Umberto
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topic_title	540 ASE 35.76 bkl The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae) Lipid transfer protein (dpeaa)DE-He213 Defense protein (dpeaa)DE-He213
topic	ddc 540 bkl 35.76 misc Lipid transfer protein misc Defense protein
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title	The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae)
ctrlnum	(DE-627)SPR014723077 (SPR)s10930-014-9575-9-e
title_full	The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae)
author_sort	Zottich, Umberto
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author_browse	Zottich, Umberto Da Cunha, Maura Dias, Germana B. Rabelo, Guilherme R. Oliveira, Antonia Elenir A. Carvalho, André O. Fernandes, Kátia Valevski S. do Nascimento, Viviane V. Gomes, Valdirene M.
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title_sort	toxicity of a lipid transfer protein (cc-ltp1) from coffea canephora seeds on the larval development of callosobruchus maculatus (coleoptera: bruchidae)
title_auth	The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae)
abstract	Abstract In this work, we analyzed the effects of coffee seed proteins, especially Cc-$ LTP_{1} $ on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-$ LTP_{1} $, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-$ LTP_{1} $ was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-$ LTP_{1} $ has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-$ LTP_{1} $ has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues.
abstractGer	Abstract In this work, we analyzed the effects of coffee seed proteins, especially Cc-$ LTP_{1} $ on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-$ LTP_{1} $, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-$ LTP_{1} $ was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-$ LTP_{1} $ has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-$ LTP_{1} $ has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues.
abstract_unstemmed	Abstract In this work, we analyzed the effects of coffee seed proteins, especially Cc-$ LTP_{1} $ on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-$ LTP_{1} $, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-$ LTP_{1} $ was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-$ LTP_{1} $ has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-$ LTP_{1} $ has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues.
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container_issue	5
title_short	The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae)
url	https://dx.doi.org/10.1007/s10930-014-9575-9
remote_bool	true
author2	Da Cunha, Maura Dias, Germana B. Rabelo, Guilherme R. Oliveira, Antonia Elenir A. Carvalho, André O. Fernandes, Kátia Valevski S. do Nascimento, Viviane V. Gomes, Valdirene M.
author2Str	Da Cunha, Maura Dias, Germana B. Rabelo, Guilherme R. Oliveira, Antonia Elenir A. Carvalho, André O. Fernandes, Kátia Valevski S. do Nascimento, Viviane V. Gomes, Valdirene M.
ppnlink	385615884
mediatype_str_mv	c
isOA_txt	false
hochschulschrift_bool	false
doi_str	10.1007/s10930-014-9575-9
up_date	2024-07-04T02:49:30.051Z
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The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae)

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