Production and evaluation of second antibody for radioimmunoassay technique
Abstract Polyclonal antibody production in mammals is generally associated with multiple injections of antigens and adjuvant and repeated blood sampling procedures. In the production of second polyclonal antibodies, a number of critical steps can be identified that may influence the outcome of the a...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Shafik, H. M. [verfasserIn] El-Mouhty, N. R. A. [verfasserIn] EL-Bayoumy, A. S. [verfasserIn] Abdel-Ghany, I. Y. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of radioanalytical and nuclear chemistry - Dordrecht [u.a.] : Springer Science + Business Media B.V., 1968, 280(2009), 3 vom: 27. Apr., Seite 613-619 |
|---|---|
| Übergeordnetes Werk: |
volume:280 ; year:2009 ; number:3 ; day:27 ; month:04 ; pages:613-619 |
| Links: |
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| DOI / URN: |
10.1007/s10967-009-7485-7 |
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| Katalog-ID: |
SPR015162303 |
|---|
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| 100 | 1 | |a Shafik, H. M. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Production and evaluation of second antibody for radioimmunoassay technique |
| 264 | 1 | |c 2009 | |
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| 520 | |a Abstract Polyclonal antibody production in mammals is generally associated with multiple injections of antigens and adjuvant and repeated blood sampling procedures. In the production of second polyclonal antibodies, a number of critical steps can be identified that may influence the outcome of the animal experiment (immunological results and the pain and suffering of the animals). The goal of this work was to evaluate critical steps in the production of these antibodies, and to optimize production protocols that will ultimately result in effective antibody responses. This work was achieved through immunization of two healthy sheep by purified Alpha feto protein (AFP) antigen. Also, the present study involved the preparation of AFP standards from human cord blood. Furthermore, preparation of a radiolabeled AFP tracer of a high specific activity using 125I isotope by chloramine T method was undertaken. Moreover, this study provides that there was no observable difference between the Scottish Antibody Production Unit (SAPU) and Sigma SAM IgG and the two second antibodies obtained from the local sheep sera. | ||
| 650 | 4 | |a Radiochemical Purity |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Radiochemical Yield |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Alpha Feto Protein |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Human Cord Blood |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Sodium Metabisulphite |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a El-Mouhty, N. R. A. |e verfasserin |4 aut | |
| 700 | 1 | |a EL-Bayoumy, A. S. |e verfasserin |4 aut | |
| 700 | 1 | |a Abdel-Ghany, I. Y. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of radioanalytical and nuclear chemistry |d Dordrecht [u.a.] : Springer Science + Business Media B.V., 1968 |g 280(2009), 3 vom: 27. Apr., Seite 613-619 |w (DE-627)320578011 |w (DE-600)2017242-4 |x 1588-2780 |7 nnns |
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| 912 | |a GBV_ILN_20 | ||
| 912 | |a GBV_ILN_22 | ||
| 912 | |a GBV_ILN_23 | ||
| 912 | |a GBV_ILN_24 | ||
| 912 | |a GBV_ILN_31 | ||
| 912 | |a GBV_ILN_32 | ||
| 912 | |a GBV_ILN_39 | ||
| 912 | |a GBV_ILN_40 | ||
| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
| 912 | |a GBV_ILN_63 | ||
| 912 | |a GBV_ILN_69 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_73 | ||
| 912 | |a GBV_ILN_74 | ||
| 912 | |a GBV_ILN_90 | ||
| 912 | |a GBV_ILN_95 | ||
| 912 | |a GBV_ILN_100 | ||
| 912 | |a GBV_ILN_101 | ||
| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_138 | ||
| 912 | |a GBV_ILN_150 | ||
| 912 | |a GBV_ILN_151 | ||
| 912 | |a GBV_ILN_152 | ||
| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
| 912 | |a GBV_ILN_171 | ||
| 912 | |a GBV_ILN_187 | ||
| 912 | |a GBV_ILN_206 | ||
| 912 | |a GBV_ILN_213 | ||
| 912 | |a GBV_ILN_224 | ||
| 912 | |a GBV_ILN_230 | ||
| 912 | |a GBV_ILN_250 | ||
| 912 | |a GBV_ILN_281 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2007 | ||
| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
| 912 | |a GBV_ILN_4700 | ||
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10.1007/s10967-009-7485-7 doi (DE-627)SPR015162303 (SPR)s10967-009-7485-7-e DE-627 ger DE-627 rakwb eng 540 ASE 35.00 bkl Shafik, H. M. verfasserin aut Production and evaluation of second antibody for radioimmunoassay technique 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Polyclonal antibody production in mammals is generally associated with multiple injections of antigens and adjuvant and repeated blood sampling procedures. In the production of second polyclonal antibodies, a number of critical steps can be identified that may influence the outcome of the animal experiment (immunological results and the pain and suffering of the animals). The goal of this work was to evaluate critical steps in the production of these antibodies, and to optimize production protocols that will ultimately result in effective antibody responses. This work was achieved through immunization of two healthy sheep by purified Alpha feto protein (AFP) antigen. Also, the present study involved the preparation of AFP standards from human cord blood. Furthermore, preparation of a radiolabeled AFP tracer of a high specific activity using 125I isotope by chloramine T method was undertaken. Moreover, this study provides that there was no observable difference between the Scottish Antibody Production Unit (SAPU) and Sigma SAM IgG and the two second antibodies obtained from the local sheep sera. Radiochemical Purity (dpeaa)DE-He213 Radiochemical Yield (dpeaa)DE-He213 Alpha Feto Protein (dpeaa)DE-He213 Human Cord Blood (dpeaa)DE-He213 Sodium Metabisulphite (dpeaa)DE-He213 El-Mouhty, N. R. A. verfasserin aut EL-Bayoumy, A. S. verfasserin aut Abdel-Ghany, I. Y. verfasserin aut Enthalten in Journal of radioanalytical and nuclear chemistry Dordrecht [u.a.] : Springer Science + Business Media B.V., 1968 280(2009), 3 vom: 27. Apr., Seite 613-619 (DE-627)320578011 (DE-600)2017242-4 1588-2780 nnns volume:280 year:2009 number:3 day:27 month:04 pages:613-619 https://dx.doi.org/10.1007/s10967-009-7485-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 ASE AR 280 2009 3 27 04 613-619 |
| spelling |
10.1007/s10967-009-7485-7 doi (DE-627)SPR015162303 (SPR)s10967-009-7485-7-e DE-627 ger DE-627 rakwb eng 540 ASE 35.00 bkl Shafik, H. M. verfasserin aut Production and evaluation of second antibody for radioimmunoassay technique 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Polyclonal antibody production in mammals is generally associated with multiple injections of antigens and adjuvant and repeated blood sampling procedures. In the production of second polyclonal antibodies, a number of critical steps can be identified that may influence the outcome of the animal experiment (immunological results and the pain and suffering of the animals). The goal of this work was to evaluate critical steps in the production of these antibodies, and to optimize production protocols that will ultimately result in effective antibody responses. This work was achieved through immunization of two healthy sheep by purified Alpha feto protein (AFP) antigen. Also, the present study involved the preparation of AFP standards from human cord blood. Furthermore, preparation of a radiolabeled AFP tracer of a high specific activity using 125I isotope by chloramine T method was undertaken. Moreover, this study provides that there was no observable difference between the Scottish Antibody Production Unit (SAPU) and Sigma SAM IgG and the two second antibodies obtained from the local sheep sera. Radiochemical Purity (dpeaa)DE-He213 Radiochemical Yield (dpeaa)DE-He213 Alpha Feto Protein (dpeaa)DE-He213 Human Cord Blood (dpeaa)DE-He213 Sodium Metabisulphite (dpeaa)DE-He213 El-Mouhty, N. R. A. verfasserin aut EL-Bayoumy, A. S. verfasserin aut Abdel-Ghany, I. Y. verfasserin aut Enthalten in Journal of radioanalytical and nuclear chemistry Dordrecht [u.a.] : Springer Science + Business Media B.V., 1968 280(2009), 3 vom: 27. Apr., Seite 613-619 (DE-627)320578011 (DE-600)2017242-4 1588-2780 nnns volume:280 year:2009 number:3 day:27 month:04 pages:613-619 https://dx.doi.org/10.1007/s10967-009-7485-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 ASE AR 280 2009 3 27 04 613-619 |
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10.1007/s10967-009-7485-7 doi (DE-627)SPR015162303 (SPR)s10967-009-7485-7-e DE-627 ger DE-627 rakwb eng 540 ASE 35.00 bkl Shafik, H. M. verfasserin aut Production and evaluation of second antibody for radioimmunoassay technique 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Polyclonal antibody production in mammals is generally associated with multiple injections of antigens and adjuvant and repeated blood sampling procedures. In the production of second polyclonal antibodies, a number of critical steps can be identified that may influence the outcome of the animal experiment (immunological results and the pain and suffering of the animals). The goal of this work was to evaluate critical steps in the production of these antibodies, and to optimize production protocols that will ultimately result in effective antibody responses. This work was achieved through immunization of two healthy sheep by purified Alpha feto protein (AFP) antigen. Also, the present study involved the preparation of AFP standards from human cord blood. Furthermore, preparation of a radiolabeled AFP tracer of a high specific activity using 125I isotope by chloramine T method was undertaken. Moreover, this study provides that there was no observable difference between the Scottish Antibody Production Unit (SAPU) and Sigma SAM IgG and the two second antibodies obtained from the local sheep sera. Radiochemical Purity (dpeaa)DE-He213 Radiochemical Yield (dpeaa)DE-He213 Alpha Feto Protein (dpeaa)DE-He213 Human Cord Blood (dpeaa)DE-He213 Sodium Metabisulphite (dpeaa)DE-He213 El-Mouhty, N. R. A. verfasserin aut EL-Bayoumy, A. S. verfasserin aut Abdel-Ghany, I. Y. verfasserin aut Enthalten in Journal of radioanalytical and nuclear chemistry Dordrecht [u.a.] : Springer Science + Business Media B.V., 1968 280(2009), 3 vom: 27. Apr., Seite 613-619 (DE-627)320578011 (DE-600)2017242-4 1588-2780 nnns volume:280 year:2009 number:3 day:27 month:04 pages:613-619 https://dx.doi.org/10.1007/s10967-009-7485-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 ASE AR 280 2009 3 27 04 613-619 |
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10.1007/s10967-009-7485-7 doi (DE-627)SPR015162303 (SPR)s10967-009-7485-7-e DE-627 ger DE-627 rakwb eng 540 ASE 35.00 bkl Shafik, H. M. verfasserin aut Production and evaluation of second antibody for radioimmunoassay technique 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Polyclonal antibody production in mammals is generally associated with multiple injections of antigens and adjuvant and repeated blood sampling procedures. In the production of second polyclonal antibodies, a number of critical steps can be identified that may influence the outcome of the animal experiment (immunological results and the pain and suffering of the animals). The goal of this work was to evaluate critical steps in the production of these antibodies, and to optimize production protocols that will ultimately result in effective antibody responses. This work was achieved through immunization of two healthy sheep by purified Alpha feto protein (AFP) antigen. Also, the present study involved the preparation of AFP standards from human cord blood. Furthermore, preparation of a radiolabeled AFP tracer of a high specific activity using 125I isotope by chloramine T method was undertaken. Moreover, this study provides that there was no observable difference between the Scottish Antibody Production Unit (SAPU) and Sigma SAM IgG and the two second antibodies obtained from the local sheep sera. Radiochemical Purity (dpeaa)DE-He213 Radiochemical Yield (dpeaa)DE-He213 Alpha Feto Protein (dpeaa)DE-He213 Human Cord Blood (dpeaa)DE-He213 Sodium Metabisulphite (dpeaa)DE-He213 El-Mouhty, N. R. A. verfasserin aut EL-Bayoumy, A. S. verfasserin aut Abdel-Ghany, I. Y. verfasserin aut Enthalten in Journal of radioanalytical and nuclear chemistry Dordrecht [u.a.] : Springer Science + Business Media B.V., 1968 280(2009), 3 vom: 27. Apr., Seite 613-619 (DE-627)320578011 (DE-600)2017242-4 1588-2780 nnns volume:280 year:2009 number:3 day:27 month:04 pages:613-619 https://dx.doi.org/10.1007/s10967-009-7485-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 ASE AR 280 2009 3 27 04 613-619 |
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10.1007/s10967-009-7485-7 doi (DE-627)SPR015162303 (SPR)s10967-009-7485-7-e DE-627 ger DE-627 rakwb eng 540 ASE 35.00 bkl Shafik, H. M. verfasserin aut Production and evaluation of second antibody for radioimmunoassay technique 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Polyclonal antibody production in mammals is generally associated with multiple injections of antigens and adjuvant and repeated blood sampling procedures. In the production of second polyclonal antibodies, a number of critical steps can be identified that may influence the outcome of the animal experiment (immunological results and the pain and suffering of the animals). The goal of this work was to evaluate critical steps in the production of these antibodies, and to optimize production protocols that will ultimately result in effective antibody responses. This work was achieved through immunization of two healthy sheep by purified Alpha feto protein (AFP) antigen. Also, the present study involved the preparation of AFP standards from human cord blood. Furthermore, preparation of a radiolabeled AFP tracer of a high specific activity using 125I isotope by chloramine T method was undertaken. Moreover, this study provides that there was no observable difference between the Scottish Antibody Production Unit (SAPU) and Sigma SAM IgG and the two second antibodies obtained from the local sheep sera. Radiochemical Purity (dpeaa)DE-He213 Radiochemical Yield (dpeaa)DE-He213 Alpha Feto Protein (dpeaa)DE-He213 Human Cord Blood (dpeaa)DE-He213 Sodium Metabisulphite (dpeaa)DE-He213 El-Mouhty, N. R. A. verfasserin aut EL-Bayoumy, A. S. verfasserin aut Abdel-Ghany, I. Y. verfasserin aut Enthalten in Journal of radioanalytical and nuclear chemistry Dordrecht [u.a.] : Springer Science + Business Media B.V., 1968 280(2009), 3 vom: 27. Apr., Seite 613-619 (DE-627)320578011 (DE-600)2017242-4 1588-2780 nnns volume:280 year:2009 number:3 day:27 month:04 pages:613-619 https://dx.doi.org/10.1007/s10967-009-7485-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 ASE AR 280 2009 3 27 04 613-619 |
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Enthalten in Journal of radioanalytical and nuclear chemistry 280(2009), 3 vom: 27. Apr., Seite 613-619 volume:280 year:2009 number:3 day:27 month:04 pages:613-619 |
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Shafik, H. M. @@aut@@ El-Mouhty, N. R. A. @@aut@@ EL-Bayoumy, A. S. @@aut@@ Abdel-Ghany, I. Y. @@aut@@ |
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Shafik, H. M. |
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Shafik, H. M. ddc 540 bkl 35.00 misc Radiochemical Purity misc Radiochemical Yield misc Alpha Feto Protein misc Human Cord Blood misc Sodium Metabisulphite Production and evaluation of second antibody for radioimmunoassay technique |
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540 ASE 35.00 bkl Production and evaluation of second antibody for radioimmunoassay technique Radiochemical Purity (dpeaa)DE-He213 Radiochemical Yield (dpeaa)DE-He213 Alpha Feto Protein (dpeaa)DE-He213 Human Cord Blood (dpeaa)DE-He213 Sodium Metabisulphite (dpeaa)DE-He213 |
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ddc 540 bkl 35.00 misc Radiochemical Purity misc Radiochemical Yield misc Alpha Feto Protein misc Human Cord Blood misc Sodium Metabisulphite |
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ddc 540 bkl 35.00 misc Radiochemical Purity misc Radiochemical Yield misc Alpha Feto Protein misc Human Cord Blood misc Sodium Metabisulphite |
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ddc 540 bkl 35.00 misc Radiochemical Purity misc Radiochemical Yield misc Alpha Feto Protein misc Human Cord Blood misc Sodium Metabisulphite |
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Production and evaluation of second antibody for radioimmunoassay technique |
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Production and evaluation of second antibody for radioimmunoassay technique |
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Shafik, H. M. |
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Journal of radioanalytical and nuclear chemistry |
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Shafik, H. M. El-Mouhty, N. R. A. EL-Bayoumy, A. S. Abdel-Ghany, I. Y. |
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Shafik, H. M. |
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verfasserin |
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production and evaluation of second antibody for radioimmunoassay technique |
| title_auth |
Production and evaluation of second antibody for radioimmunoassay technique |
| abstract |
Abstract Polyclonal antibody production in mammals is generally associated with multiple injections of antigens and adjuvant and repeated blood sampling procedures. In the production of second polyclonal antibodies, a number of critical steps can be identified that may influence the outcome of the animal experiment (immunological results and the pain and suffering of the animals). The goal of this work was to evaluate critical steps in the production of these antibodies, and to optimize production protocols that will ultimately result in effective antibody responses. This work was achieved through immunization of two healthy sheep by purified Alpha feto protein (AFP) antigen. Also, the present study involved the preparation of AFP standards from human cord blood. Furthermore, preparation of a radiolabeled AFP tracer of a high specific activity using 125I isotope by chloramine T method was undertaken. Moreover, this study provides that there was no observable difference between the Scottish Antibody Production Unit (SAPU) and Sigma SAM IgG and the two second antibodies obtained from the local sheep sera. |
| abstractGer |
Abstract Polyclonal antibody production in mammals is generally associated with multiple injections of antigens and adjuvant and repeated blood sampling procedures. In the production of second polyclonal antibodies, a number of critical steps can be identified that may influence the outcome of the animal experiment (immunological results and the pain and suffering of the animals). The goal of this work was to evaluate critical steps in the production of these antibodies, and to optimize production protocols that will ultimately result in effective antibody responses. This work was achieved through immunization of two healthy sheep by purified Alpha feto protein (AFP) antigen. Also, the present study involved the preparation of AFP standards from human cord blood. Furthermore, preparation of a radiolabeled AFP tracer of a high specific activity using 125I isotope by chloramine T method was undertaken. Moreover, this study provides that there was no observable difference between the Scottish Antibody Production Unit (SAPU) and Sigma SAM IgG and the two second antibodies obtained from the local sheep sera. |
| abstract_unstemmed |
Abstract Polyclonal antibody production in mammals is generally associated with multiple injections of antigens and adjuvant and repeated blood sampling procedures. In the production of second polyclonal antibodies, a number of critical steps can be identified that may influence the outcome of the animal experiment (immunological results and the pain and suffering of the animals). The goal of this work was to evaluate critical steps in the production of these antibodies, and to optimize production protocols that will ultimately result in effective antibody responses. This work was achieved through immunization of two healthy sheep by purified Alpha feto protein (AFP) antigen. Also, the present study involved the preparation of AFP standards from human cord blood. Furthermore, preparation of a radiolabeled AFP tracer of a high specific activity using 125I isotope by chloramine T method was undertaken. Moreover, this study provides that there was no observable difference between the Scottish Antibody Production Unit (SAPU) and Sigma SAM IgG and the two second antibodies obtained from the local sheep sera. |
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| title_short |
Production and evaluation of second antibody for radioimmunoassay technique |
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https://dx.doi.org/10.1007/s10967-009-7485-7 |
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El-Mouhty, N. R. A. EL-Bayoumy, A. S. Abdel-Ghany, I. Y. |
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El-Mouhty, N. R. A. EL-Bayoumy, A. S. Abdel-Ghany, I. Y. |
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10.1007/s10967-009-7485-7 |
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2024-07-03T14:20:13.055Z |
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| score |
7.3985195 |