Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster
Abstract Eu-heterochromatic rearrangements transfer genes into the heterochromatin and cause their variegated inactivation (PEV). Genes affected by PEV often demonstrate association with heterochromatic nuclear compartment (a distinct area composed of heterochromatin sequences like satellite DNA and...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lavrov, S. A. [verfasserIn] Shatskikh, A. S. [verfasserIn] Kibanov, M. V. [verfasserIn] Gvozdev, V. A. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Molecular biology - Moscow : MAIK Nauka/Interperiodica Publ., 1997, 47(2013), 2 vom: März, Seite 252-258 |
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| Übergeordnetes Werk: |
volume:47 ; year:2013 ; number:2 ; month:03 ; pages:252-258 |
| Links: |
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| DOI / URN: |
10.1134/S0026893313020088 |
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| Katalog-ID: |
SPR015587460 |
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| 100 | 1 | |a Lavrov, S. A. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster |
| 264 | 1 | |c 2013 | |
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| 520 | |a Abstract Eu-heterochromatic rearrangements transfer genes into the heterochromatin and cause their variegated inactivation (PEV). Genes affected by PEV often demonstrate association with heterochromatic nuclear compartment (a distinct area composed of heterochromatin sequences like satellite DNA and enriched in specific chromatin proteins, e.g., HP1). Here, we investigate the nuclear localization and the expression levels of the genes subjected to PEV caused by chromosome inversion, In(2)A4. We demonstrate that the degree of PEV-caused gene inactivation depends on a developmental stage, and the maximum of repression corresponds to the gene expression activation period. In the case of In(2)A4 rearrangement, we detect the dragging of the affected euchromatin region into the nuclear compartment of heterochromatin and the increase in HP1 occupancy in this region. We developed a protocol for simultaneous RNA-DNA-protein staining in order to demonstrate the strong correlation between the transcriptional activity of the affected gene and its distance from chromosome 2 satellite DNA in a single cell. | ||
| 650 | 4 | |a drosophila |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a heterochromatin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a PEV |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a transcription |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a nuclear structure |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a in situ hybridization |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Shatskikh, A. S. |e verfasserin |4 aut | |
| 700 | 1 | |a Kibanov, M. V. |e verfasserin |4 aut | |
| 700 | 1 | |a Gvozdev, V. A. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular biology |d Moscow : MAIK Nauka/Interperiodica Publ., 1997 |g 47(2013), 2 vom: März, Seite 252-258 |w (DE-627)324825382 |w (DE-600)2031117-5 |x 1608-3245 |7 nnns |
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| 912 | |a GBV_ILN_2037 | ||
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| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
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| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
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| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
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| 912 | |a GBV_ILN_4325 | ||
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10.1134/S0026893313020088 doi (DE-627)SPR015587460 (SPR)S0026893313020088-e DE-627 ger DE-627 rakwb eng 570 ASE 42.00 bkl Lavrov, S. A. verfasserin aut Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Eu-heterochromatic rearrangements transfer genes into the heterochromatin and cause their variegated inactivation (PEV). Genes affected by PEV often demonstrate association with heterochromatic nuclear compartment (a distinct area composed of heterochromatin sequences like satellite DNA and enriched in specific chromatin proteins, e.g., HP1). Here, we investigate the nuclear localization and the expression levels of the genes subjected to PEV caused by chromosome inversion, In(2)A4. We demonstrate that the degree of PEV-caused gene inactivation depends on a developmental stage, and the maximum of repression corresponds to the gene expression activation period. In the case of In(2)A4 rearrangement, we detect the dragging of the affected euchromatin region into the nuclear compartment of heterochromatin and the increase in HP1 occupancy in this region. We developed a protocol for simultaneous RNA-DNA-protein staining in order to demonstrate the strong correlation between the transcriptional activity of the affected gene and its distance from chromosome 2 satellite DNA in a single cell. drosophila (dpeaa)DE-He213 heterochromatin (dpeaa)DE-He213 PEV (dpeaa)DE-He213 transcription (dpeaa)DE-He213 nuclear structure (dpeaa)DE-He213 in situ hybridization (dpeaa)DE-He213 Shatskikh, A. S. verfasserin aut Kibanov, M. V. verfasserin aut Gvozdev, V. A. verfasserin aut Enthalten in Molecular biology Moscow : MAIK Nauka/Interperiodica Publ., 1997 47(2013), 2 vom: März, Seite 252-258 (DE-627)324825382 (DE-600)2031117-5 1608-3245 nnns volume:47 year:2013 number:2 month:03 pages:252-258 https://dx.doi.org/10.1134/S0026893313020088 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE AR 47 2013 2 03 252-258 |
| spelling |
10.1134/S0026893313020088 doi (DE-627)SPR015587460 (SPR)S0026893313020088-e DE-627 ger DE-627 rakwb eng 570 ASE 42.00 bkl Lavrov, S. A. verfasserin aut Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Eu-heterochromatic rearrangements transfer genes into the heterochromatin and cause their variegated inactivation (PEV). Genes affected by PEV often demonstrate association with heterochromatic nuclear compartment (a distinct area composed of heterochromatin sequences like satellite DNA and enriched in specific chromatin proteins, e.g., HP1). Here, we investigate the nuclear localization and the expression levels of the genes subjected to PEV caused by chromosome inversion, In(2)A4. We demonstrate that the degree of PEV-caused gene inactivation depends on a developmental stage, and the maximum of repression corresponds to the gene expression activation period. In the case of In(2)A4 rearrangement, we detect the dragging of the affected euchromatin region into the nuclear compartment of heterochromatin and the increase in HP1 occupancy in this region. We developed a protocol for simultaneous RNA-DNA-protein staining in order to demonstrate the strong correlation between the transcriptional activity of the affected gene and its distance from chromosome 2 satellite DNA in a single cell. drosophila (dpeaa)DE-He213 heterochromatin (dpeaa)DE-He213 PEV (dpeaa)DE-He213 transcription (dpeaa)DE-He213 nuclear structure (dpeaa)DE-He213 in situ hybridization (dpeaa)DE-He213 Shatskikh, A. S. verfasserin aut Kibanov, M. V. verfasserin aut Gvozdev, V. A. verfasserin aut Enthalten in Molecular biology Moscow : MAIK Nauka/Interperiodica Publ., 1997 47(2013), 2 vom: März, Seite 252-258 (DE-627)324825382 (DE-600)2031117-5 1608-3245 nnns volume:47 year:2013 number:2 month:03 pages:252-258 https://dx.doi.org/10.1134/S0026893313020088 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE AR 47 2013 2 03 252-258 |
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10.1134/S0026893313020088 doi (DE-627)SPR015587460 (SPR)S0026893313020088-e DE-627 ger DE-627 rakwb eng 570 ASE 42.00 bkl Lavrov, S. A. verfasserin aut Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Eu-heterochromatic rearrangements transfer genes into the heterochromatin and cause their variegated inactivation (PEV). Genes affected by PEV often demonstrate association with heterochromatic nuclear compartment (a distinct area composed of heterochromatin sequences like satellite DNA and enriched in specific chromatin proteins, e.g., HP1). Here, we investigate the nuclear localization and the expression levels of the genes subjected to PEV caused by chromosome inversion, In(2)A4. We demonstrate that the degree of PEV-caused gene inactivation depends on a developmental stage, and the maximum of repression corresponds to the gene expression activation period. In the case of In(2)A4 rearrangement, we detect the dragging of the affected euchromatin region into the nuclear compartment of heterochromatin and the increase in HP1 occupancy in this region. We developed a protocol for simultaneous RNA-DNA-protein staining in order to demonstrate the strong correlation between the transcriptional activity of the affected gene and its distance from chromosome 2 satellite DNA in a single cell. drosophila (dpeaa)DE-He213 heterochromatin (dpeaa)DE-He213 PEV (dpeaa)DE-He213 transcription (dpeaa)DE-He213 nuclear structure (dpeaa)DE-He213 in situ hybridization (dpeaa)DE-He213 Shatskikh, A. S. verfasserin aut Kibanov, M. V. verfasserin aut Gvozdev, V. A. verfasserin aut Enthalten in Molecular biology Moscow : MAIK Nauka/Interperiodica Publ., 1997 47(2013), 2 vom: März, Seite 252-258 (DE-627)324825382 (DE-600)2031117-5 1608-3245 nnns volume:47 year:2013 number:2 month:03 pages:252-258 https://dx.doi.org/10.1134/S0026893313020088 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE AR 47 2013 2 03 252-258 |
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10.1134/S0026893313020088 doi (DE-627)SPR015587460 (SPR)S0026893313020088-e DE-627 ger DE-627 rakwb eng 570 ASE 42.00 bkl Lavrov, S. A. verfasserin aut Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Eu-heterochromatic rearrangements transfer genes into the heterochromatin and cause their variegated inactivation (PEV). Genes affected by PEV often demonstrate association with heterochromatic nuclear compartment (a distinct area composed of heterochromatin sequences like satellite DNA and enriched in specific chromatin proteins, e.g., HP1). Here, we investigate the nuclear localization and the expression levels of the genes subjected to PEV caused by chromosome inversion, In(2)A4. We demonstrate that the degree of PEV-caused gene inactivation depends on a developmental stage, and the maximum of repression corresponds to the gene expression activation period. In the case of In(2)A4 rearrangement, we detect the dragging of the affected euchromatin region into the nuclear compartment of heterochromatin and the increase in HP1 occupancy in this region. We developed a protocol for simultaneous RNA-DNA-protein staining in order to demonstrate the strong correlation between the transcriptional activity of the affected gene and its distance from chromosome 2 satellite DNA in a single cell. drosophila (dpeaa)DE-He213 heterochromatin (dpeaa)DE-He213 PEV (dpeaa)DE-He213 transcription (dpeaa)DE-He213 nuclear structure (dpeaa)DE-He213 in situ hybridization (dpeaa)DE-He213 Shatskikh, A. S. verfasserin aut Kibanov, M. V. verfasserin aut Gvozdev, V. A. verfasserin aut Enthalten in Molecular biology Moscow : MAIK Nauka/Interperiodica Publ., 1997 47(2013), 2 vom: März, Seite 252-258 (DE-627)324825382 (DE-600)2031117-5 1608-3245 nnns volume:47 year:2013 number:2 month:03 pages:252-258 https://dx.doi.org/10.1134/S0026893313020088 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE AR 47 2013 2 03 252-258 |
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10.1134/S0026893313020088 doi (DE-627)SPR015587460 (SPR)S0026893313020088-e DE-627 ger DE-627 rakwb eng 570 ASE 42.00 bkl Lavrov, S. A. verfasserin aut Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Eu-heterochromatic rearrangements transfer genes into the heterochromatin and cause their variegated inactivation (PEV). Genes affected by PEV often demonstrate association with heterochromatic nuclear compartment (a distinct area composed of heterochromatin sequences like satellite DNA and enriched in specific chromatin proteins, e.g., HP1). Here, we investigate the nuclear localization and the expression levels of the genes subjected to PEV caused by chromosome inversion, In(2)A4. We demonstrate that the degree of PEV-caused gene inactivation depends on a developmental stage, and the maximum of repression corresponds to the gene expression activation period. In the case of In(2)A4 rearrangement, we detect the dragging of the affected euchromatin region into the nuclear compartment of heterochromatin and the increase in HP1 occupancy in this region. We developed a protocol for simultaneous RNA-DNA-protein staining in order to demonstrate the strong correlation between the transcriptional activity of the affected gene and its distance from chromosome 2 satellite DNA in a single cell. drosophila (dpeaa)DE-He213 heterochromatin (dpeaa)DE-He213 PEV (dpeaa)DE-He213 transcription (dpeaa)DE-He213 nuclear structure (dpeaa)DE-He213 in situ hybridization (dpeaa)DE-He213 Shatskikh, A. S. verfasserin aut Kibanov, M. V. verfasserin aut Gvozdev, V. A. verfasserin aut Enthalten in Molecular biology Moscow : MAIK Nauka/Interperiodica Publ., 1997 47(2013), 2 vom: März, Seite 252-258 (DE-627)324825382 (DE-600)2031117-5 1608-3245 nnns volume:47 year:2013 number:2 month:03 pages:252-258 https://dx.doi.org/10.1134/S0026893313020088 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE AR 47 2013 2 03 252-258 |
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Enthalten in Molecular biology 47(2013), 2 vom: März, Seite 252-258 volume:47 year:2013 number:2 month:03 pages:252-258 |
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drosophila heterochromatin PEV transcription nuclear structure in situ hybridization |
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Lavrov, S. A. @@aut@@ Shatskikh, A. S. @@aut@@ Kibanov, M. V. @@aut@@ Gvozdev, V. A. @@aut@@ |
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Lavrov, S. A. |
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Lavrov, S. A. ddc 570 bkl 42.00 misc drosophila misc heterochromatin misc PEV misc transcription misc nuclear structure misc in situ hybridization Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster |
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570 ASE 42.00 bkl Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster drosophila (dpeaa)DE-He213 heterochromatin (dpeaa)DE-He213 PEV (dpeaa)DE-He213 transcription (dpeaa)DE-He213 nuclear structure (dpeaa)DE-He213 in situ hybridization (dpeaa)DE-He213 |
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ddc 570 bkl 42.00 misc drosophila misc heterochromatin misc PEV misc transcription misc nuclear structure misc in situ hybridization |
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Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster |
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Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster |
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Lavrov, S. A. |
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correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of pev-producing eu-heterochromatin rearrangement in drosophila melanogaster |
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Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster |
| abstract |
Abstract Eu-heterochromatic rearrangements transfer genes into the heterochromatin and cause their variegated inactivation (PEV). Genes affected by PEV often demonstrate association with heterochromatic nuclear compartment (a distinct area composed of heterochromatin sequences like satellite DNA and enriched in specific chromatin proteins, e.g., HP1). Here, we investigate the nuclear localization and the expression levels of the genes subjected to PEV caused by chromosome inversion, In(2)A4. We demonstrate that the degree of PEV-caused gene inactivation depends on a developmental stage, and the maximum of repression corresponds to the gene expression activation period. In the case of In(2)A4 rearrangement, we detect the dragging of the affected euchromatin region into the nuclear compartment of heterochromatin and the increase in HP1 occupancy in this region. We developed a protocol for simultaneous RNA-DNA-protein staining in order to demonstrate the strong correlation between the transcriptional activity of the affected gene and its distance from chromosome 2 satellite DNA in a single cell. |
| abstractGer |
Abstract Eu-heterochromatic rearrangements transfer genes into the heterochromatin and cause their variegated inactivation (PEV). Genes affected by PEV often demonstrate association with heterochromatic nuclear compartment (a distinct area composed of heterochromatin sequences like satellite DNA and enriched in specific chromatin proteins, e.g., HP1). Here, we investigate the nuclear localization and the expression levels of the genes subjected to PEV caused by chromosome inversion, In(2)A4. We demonstrate that the degree of PEV-caused gene inactivation depends on a developmental stage, and the maximum of repression corresponds to the gene expression activation period. In the case of In(2)A4 rearrangement, we detect the dragging of the affected euchromatin region into the nuclear compartment of heterochromatin and the increase in HP1 occupancy in this region. We developed a protocol for simultaneous RNA-DNA-protein staining in order to demonstrate the strong correlation between the transcriptional activity of the affected gene and its distance from chromosome 2 satellite DNA in a single cell. |
| abstract_unstemmed |
Abstract Eu-heterochromatic rearrangements transfer genes into the heterochromatin and cause their variegated inactivation (PEV). Genes affected by PEV often demonstrate association with heterochromatic nuclear compartment (a distinct area composed of heterochromatin sequences like satellite DNA and enriched in specific chromatin proteins, e.g., HP1). Here, we investigate the nuclear localization and the expression levels of the genes subjected to PEV caused by chromosome inversion, In(2)A4. We demonstrate that the degree of PEV-caused gene inactivation depends on a developmental stage, and the maximum of repression corresponds to the gene expression activation period. In the case of In(2)A4 rearrangement, we detect the dragging of the affected euchromatin region into the nuclear compartment of heterochromatin and the increase in HP1 occupancy in this region. We developed a protocol for simultaneous RNA-DNA-protein staining in order to demonstrate the strong correlation between the transcriptional activity of the affected gene and its distance from chromosome 2 satellite DNA in a single cell. |
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| title_short |
Correlation between cellular level of gene transcriptional silencing and heterochromatin compartment dragging in case of PEV-producing eu-heterochromatin rearrangement in Drosophila melanogaster |
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https://dx.doi.org/10.1134/S0026893313020088 |
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| author2 |
Shatskikh, A. S. Kibanov, M. V. Gvozdev, V. A. |
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Shatskikh, A. S. Kibanov, M. V. Gvozdev, V. A. |
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| doi_str |
10.1134/S0026893313020088 |
| up_date |
2024-07-03T17:11:55.998Z |
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1803578733754318848 |
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|
| score |
7.4003134 |