DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids
Abstract Heterosis is prevalent in Larix kaempferi, but the molecular mechanism underlying heterosis in this species has not yet been elucidated. We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Lar...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Li, Ai [verfasserIn] Song, Wen-Qin [verfasserIn] Chen, Cheng-Bin [verfasserIn] Zhou, Ya-Nan [verfasserIn] Qi, Li-Wang [verfasserIn] Wang, Chun-Guo [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2012 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Molecular breeding - Dordrecht : Springer Science + Business Media B.V., 1995, 31(2012), 2 vom: 05. Dez., Seite 463-475 |
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| Übergeordnetes Werk: |
volume:31 ; year:2012 ; number:2 ; day:05 ; month:12 ; pages:463-475 |
| Links: |
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| DOI / URN: |
10.1007/s11032-012-9803-y |
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| Katalog-ID: |
SPR015851931 |
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| 245 | 1 | 0 | |a DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids |
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| 520 | |a Abstract Heterosis is prevalent in Larix kaempferi, but the molecular mechanism underlying heterosis in this species has not yet been elucidated. We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Larix kaempferi intraspecific parental lines and their reciprocal hybrids. The results indicate that the two intraspecific parental lines have highly similar genome structures and that the crosses did not produce substantial changes in the genomic DNA of the hybrid progeny. In contrast, there was a significant difference in the DNA methylation status between the two parental lines. The genomic DNA methylation level in the heterotic hybrids (26.47 %) was significantly lower than the midparent value (MPV) (33.80 %), indicating that significant extensive demethylation had occurred. However, the non-heterotic hybrids displayed a DNA methylation level (33.59 %) that was close to the MPV, and they contained similar numbers of methylation and demethylation events. The correlation analysis revealed that the increased gene expression in the heterotic hybrids was associated with its overall low genomic DNA methylation level and dynamic DNA methylation patterns. Twenty fragments with adjusted DNA methylation patterns were then cloned and the transcript level analyzed quantitatively. The results further indicated that the expressions of most but not all of the 12 possible genes were negatively correlated to their respective DNA methylation status. Based on these data, we suggest that adjustments in DNA methylation status may play critical roles in the formation of heterosis produced by the cross between two Larix kaempferi intraspecific parental lines. | ||
| 650 | 4 | |a (Lamb.) Carr |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a DNA methylation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Heterosis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Gene expression profiling |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Song, Wen-Qin |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Cheng-Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Ya-Nan |e verfasserin |4 aut | |
| 700 | 1 | |a Qi, Li-Wang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Chun-Guo |e verfasserin |4 aut | |
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10.1007/s11032-012-9803-y doi (DE-627)SPR015851931 (SPR)s11032-012-9803-y-e DE-627 ger DE-627 rakwb eng 580 ASE 48.58 bkl 42.43 bkl Li, Ai verfasserin aut DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Heterosis is prevalent in Larix kaempferi, but the molecular mechanism underlying heterosis in this species has not yet been elucidated. We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Larix kaempferi intraspecific parental lines and their reciprocal hybrids. The results indicate that the two intraspecific parental lines have highly similar genome structures and that the crosses did not produce substantial changes in the genomic DNA of the hybrid progeny. In contrast, there was a significant difference in the DNA methylation status between the two parental lines. The genomic DNA methylation level in the heterotic hybrids (26.47 %) was significantly lower than the midparent value (MPV) (33.80 %), indicating that significant extensive demethylation had occurred. However, the non-heterotic hybrids displayed a DNA methylation level (33.59 %) that was close to the MPV, and they contained similar numbers of methylation and demethylation events. The correlation analysis revealed that the increased gene expression in the heterotic hybrids was associated with its overall low genomic DNA methylation level and dynamic DNA methylation patterns. Twenty fragments with adjusted DNA methylation patterns were then cloned and the transcript level analyzed quantitatively. The results further indicated that the expressions of most but not all of the 12 possible genes were negatively correlated to their respective DNA methylation status. Based on these data, we suggest that adjustments in DNA methylation status may play critical roles in the formation of heterosis produced by the cross between two Larix kaempferi intraspecific parental lines. (Lamb.) Carr (dpeaa)DE-He213 DNA methylation (dpeaa)DE-He213 Heterosis (dpeaa)DE-He213 Gene expression profiling (dpeaa)DE-He213 Song, Wen-Qin verfasserin aut Chen, Cheng-Bin verfasserin aut Zhou, Ya-Nan verfasserin aut Qi, Li-Wang verfasserin aut Wang, Chun-Guo verfasserin aut Enthalten in Molecular breeding Dordrecht : Springer Science + Business Media B.V., 1995 31(2012), 2 vom: 05. Dez., Seite 463-475 (DE-627)270930671 (DE-600)1478220-0 1572-9788 nnns volume:31 year:2012 number:2 day:05 month:12 pages:463-475 https://dx.doi.org/10.1007/s11032-012-9803-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.58 ASE 42.43 ASE AR 31 2012 2 05 12 463-475 |
| spelling |
10.1007/s11032-012-9803-y doi (DE-627)SPR015851931 (SPR)s11032-012-9803-y-e DE-627 ger DE-627 rakwb eng 580 ASE 48.58 bkl 42.43 bkl Li, Ai verfasserin aut DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Heterosis is prevalent in Larix kaempferi, but the molecular mechanism underlying heterosis in this species has not yet been elucidated. We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Larix kaempferi intraspecific parental lines and their reciprocal hybrids. The results indicate that the two intraspecific parental lines have highly similar genome structures and that the crosses did not produce substantial changes in the genomic DNA of the hybrid progeny. In contrast, there was a significant difference in the DNA methylation status between the two parental lines. The genomic DNA methylation level in the heterotic hybrids (26.47 %) was significantly lower than the midparent value (MPV) (33.80 %), indicating that significant extensive demethylation had occurred. However, the non-heterotic hybrids displayed a DNA methylation level (33.59 %) that was close to the MPV, and they contained similar numbers of methylation and demethylation events. The correlation analysis revealed that the increased gene expression in the heterotic hybrids was associated with its overall low genomic DNA methylation level and dynamic DNA methylation patterns. Twenty fragments with adjusted DNA methylation patterns were then cloned and the transcript level analyzed quantitatively. The results further indicated that the expressions of most but not all of the 12 possible genes were negatively correlated to their respective DNA methylation status. Based on these data, we suggest that adjustments in DNA methylation status may play critical roles in the formation of heterosis produced by the cross between two Larix kaempferi intraspecific parental lines. (Lamb.) Carr (dpeaa)DE-He213 DNA methylation (dpeaa)DE-He213 Heterosis (dpeaa)DE-He213 Gene expression profiling (dpeaa)DE-He213 Song, Wen-Qin verfasserin aut Chen, Cheng-Bin verfasserin aut Zhou, Ya-Nan verfasserin aut Qi, Li-Wang verfasserin aut Wang, Chun-Guo verfasserin aut Enthalten in Molecular breeding Dordrecht : Springer Science + Business Media B.V., 1995 31(2012), 2 vom: 05. Dez., Seite 463-475 (DE-627)270930671 (DE-600)1478220-0 1572-9788 nnns volume:31 year:2012 number:2 day:05 month:12 pages:463-475 https://dx.doi.org/10.1007/s11032-012-9803-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.58 ASE 42.43 ASE AR 31 2012 2 05 12 463-475 |
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10.1007/s11032-012-9803-y doi (DE-627)SPR015851931 (SPR)s11032-012-9803-y-e DE-627 ger DE-627 rakwb eng 580 ASE 48.58 bkl 42.43 bkl Li, Ai verfasserin aut DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Heterosis is prevalent in Larix kaempferi, but the molecular mechanism underlying heterosis in this species has not yet been elucidated. We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Larix kaempferi intraspecific parental lines and their reciprocal hybrids. The results indicate that the two intraspecific parental lines have highly similar genome structures and that the crosses did not produce substantial changes in the genomic DNA of the hybrid progeny. In contrast, there was a significant difference in the DNA methylation status between the two parental lines. The genomic DNA methylation level in the heterotic hybrids (26.47 %) was significantly lower than the midparent value (MPV) (33.80 %), indicating that significant extensive demethylation had occurred. However, the non-heterotic hybrids displayed a DNA methylation level (33.59 %) that was close to the MPV, and they contained similar numbers of methylation and demethylation events. The correlation analysis revealed that the increased gene expression in the heterotic hybrids was associated with its overall low genomic DNA methylation level and dynamic DNA methylation patterns. Twenty fragments with adjusted DNA methylation patterns were then cloned and the transcript level analyzed quantitatively. The results further indicated that the expressions of most but not all of the 12 possible genes were negatively correlated to their respective DNA methylation status. Based on these data, we suggest that adjustments in DNA methylation status may play critical roles in the formation of heterosis produced by the cross between two Larix kaempferi intraspecific parental lines. (Lamb.) Carr (dpeaa)DE-He213 DNA methylation (dpeaa)DE-He213 Heterosis (dpeaa)DE-He213 Gene expression profiling (dpeaa)DE-He213 Song, Wen-Qin verfasserin aut Chen, Cheng-Bin verfasserin aut Zhou, Ya-Nan verfasserin aut Qi, Li-Wang verfasserin aut Wang, Chun-Guo verfasserin aut Enthalten in Molecular breeding Dordrecht : Springer Science + Business Media B.V., 1995 31(2012), 2 vom: 05. Dez., Seite 463-475 (DE-627)270930671 (DE-600)1478220-0 1572-9788 nnns volume:31 year:2012 number:2 day:05 month:12 pages:463-475 https://dx.doi.org/10.1007/s11032-012-9803-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.58 ASE 42.43 ASE AR 31 2012 2 05 12 463-475 |
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10.1007/s11032-012-9803-y doi (DE-627)SPR015851931 (SPR)s11032-012-9803-y-e DE-627 ger DE-627 rakwb eng 580 ASE 48.58 bkl 42.43 bkl Li, Ai verfasserin aut DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Heterosis is prevalent in Larix kaempferi, but the molecular mechanism underlying heterosis in this species has not yet been elucidated. We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Larix kaempferi intraspecific parental lines and their reciprocal hybrids. The results indicate that the two intraspecific parental lines have highly similar genome structures and that the crosses did not produce substantial changes in the genomic DNA of the hybrid progeny. In contrast, there was a significant difference in the DNA methylation status between the two parental lines. The genomic DNA methylation level in the heterotic hybrids (26.47 %) was significantly lower than the midparent value (MPV) (33.80 %), indicating that significant extensive demethylation had occurred. However, the non-heterotic hybrids displayed a DNA methylation level (33.59 %) that was close to the MPV, and they contained similar numbers of methylation and demethylation events. The correlation analysis revealed that the increased gene expression in the heterotic hybrids was associated with its overall low genomic DNA methylation level and dynamic DNA methylation patterns. Twenty fragments with adjusted DNA methylation patterns were then cloned and the transcript level analyzed quantitatively. The results further indicated that the expressions of most but not all of the 12 possible genes were negatively correlated to their respective DNA methylation status. Based on these data, we suggest that adjustments in DNA methylation status may play critical roles in the formation of heterosis produced by the cross between two Larix kaempferi intraspecific parental lines. (Lamb.) Carr (dpeaa)DE-He213 DNA methylation (dpeaa)DE-He213 Heterosis (dpeaa)DE-He213 Gene expression profiling (dpeaa)DE-He213 Song, Wen-Qin verfasserin aut Chen, Cheng-Bin verfasserin aut Zhou, Ya-Nan verfasserin aut Qi, Li-Wang verfasserin aut Wang, Chun-Guo verfasserin aut Enthalten in Molecular breeding Dordrecht : Springer Science + Business Media B.V., 1995 31(2012), 2 vom: 05. Dez., Seite 463-475 (DE-627)270930671 (DE-600)1478220-0 1572-9788 nnns volume:31 year:2012 number:2 day:05 month:12 pages:463-475 https://dx.doi.org/10.1007/s11032-012-9803-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.58 ASE 42.43 ASE AR 31 2012 2 05 12 463-475 |
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10.1007/s11032-012-9803-y doi (DE-627)SPR015851931 (SPR)s11032-012-9803-y-e DE-627 ger DE-627 rakwb eng 580 ASE 48.58 bkl 42.43 bkl Li, Ai verfasserin aut DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Heterosis is prevalent in Larix kaempferi, but the molecular mechanism underlying heterosis in this species has not yet been elucidated. We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Larix kaempferi intraspecific parental lines and their reciprocal hybrids. The results indicate that the two intraspecific parental lines have highly similar genome structures and that the crosses did not produce substantial changes in the genomic DNA of the hybrid progeny. In contrast, there was a significant difference in the DNA methylation status between the two parental lines. The genomic DNA methylation level in the heterotic hybrids (26.47 %) was significantly lower than the midparent value (MPV) (33.80 %), indicating that significant extensive demethylation had occurred. However, the non-heterotic hybrids displayed a DNA methylation level (33.59 %) that was close to the MPV, and they contained similar numbers of methylation and demethylation events. The correlation analysis revealed that the increased gene expression in the heterotic hybrids was associated with its overall low genomic DNA methylation level and dynamic DNA methylation patterns. Twenty fragments with adjusted DNA methylation patterns were then cloned and the transcript level analyzed quantitatively. The results further indicated that the expressions of most but not all of the 12 possible genes were negatively correlated to their respective DNA methylation status. Based on these data, we suggest that adjustments in DNA methylation status may play critical roles in the formation of heterosis produced by the cross between two Larix kaempferi intraspecific parental lines. (Lamb.) Carr (dpeaa)DE-He213 DNA methylation (dpeaa)DE-He213 Heterosis (dpeaa)DE-He213 Gene expression profiling (dpeaa)DE-He213 Song, Wen-Qin verfasserin aut Chen, Cheng-Bin verfasserin aut Zhou, Ya-Nan verfasserin aut Qi, Li-Wang verfasserin aut Wang, Chun-Guo verfasserin aut Enthalten in Molecular breeding Dordrecht : Springer Science + Business Media B.V., 1995 31(2012), 2 vom: 05. Dez., Seite 463-475 (DE-627)270930671 (DE-600)1478220-0 1572-9788 nnns volume:31 year:2012 number:2 day:05 month:12 pages:463-475 https://dx.doi.org/10.1007/s11032-012-9803-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.58 ASE 42.43 ASE AR 31 2012 2 05 12 463-475 |
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Enthalten in Molecular breeding 31(2012), 2 vom: 05. Dez., Seite 463-475 volume:31 year:2012 number:2 day:05 month:12 pages:463-475 |
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(Lamb.) Carr DNA methylation Heterosis Gene expression profiling |
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Li, Ai @@aut@@ Song, Wen-Qin @@aut@@ Chen, Cheng-Bin @@aut@@ Zhou, Ya-Nan @@aut@@ Qi, Li-Wang @@aut@@ Wang, Chun-Guo @@aut@@ |
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We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Larix kaempferi intraspecific parental lines and their reciprocal hybrids. The results indicate that the two intraspecific parental lines have highly similar genome structures and that the crosses did not produce substantial changes in the genomic DNA of the hybrid progeny. In contrast, there was a significant difference in the DNA methylation status between the two parental lines. The genomic DNA methylation level in the heterotic hybrids (26.47 %) was significantly lower than the midparent value (MPV) (33.80 %), indicating that significant extensive demethylation had occurred. However, the non-heterotic hybrids displayed a DNA methylation level (33.59 %) that was close to the MPV, and they contained similar numbers of methylation and demethylation events. The correlation analysis revealed that the increased gene expression in the heterotic hybrids was associated with its overall low genomic DNA methylation level and dynamic DNA methylation patterns. Twenty fragments with adjusted DNA methylation patterns were then cloned and the transcript level analyzed quantitatively. The results further indicated that the expressions of most but not all of the 12 possible genes were negatively correlated to their respective DNA methylation status. Based on these data, we suggest that adjustments in DNA methylation status may play critical roles in the formation of heterosis produced by the cross between two Larix kaempferi intraspecific parental lines.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">(Lamb.) Carr</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">DNA methylation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Heterosis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gene expression profiling</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Song, Wen-Qin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chen, Cheng-Bin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhou, Ya-Nan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Qi, Li-Wang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Chun-Guo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Molecular breeding</subfield><subfield code="d">Dordrecht : Springer Science + Business Media B.V., 1995</subfield><subfield code="g">31(2012), 2 vom: 05. 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|
| author |
Li, Ai |
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Li, Ai ddc 580 bkl 48.58 bkl 42.43 misc (Lamb.) Carr misc DNA methylation misc Heterosis misc Gene expression profiling DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids |
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580 ASE 48.58 bkl 42.43 bkl DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids (Lamb.) Carr (dpeaa)DE-He213 DNA methylation (dpeaa)DE-He213 Heterosis (dpeaa)DE-He213 Gene expression profiling (dpeaa)DE-He213 |
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ddc 580 bkl 48.58 bkl 42.43 misc (Lamb.) Carr misc DNA methylation misc Heterosis misc Gene expression profiling |
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DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids |
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verfasserin |
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dna methylation status is associated with the formation of heterosis in larix kaempferi intraspecific hybrids |
| title_auth |
DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids |
| abstract |
Abstract Heterosis is prevalent in Larix kaempferi, but the molecular mechanism underlying heterosis in this species has not yet been elucidated. We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Larix kaempferi intraspecific parental lines and their reciprocal hybrids. The results indicate that the two intraspecific parental lines have highly similar genome structures and that the crosses did not produce substantial changes in the genomic DNA of the hybrid progeny. In contrast, there was a significant difference in the DNA methylation status between the two parental lines. The genomic DNA methylation level in the heterotic hybrids (26.47 %) was significantly lower than the midparent value (MPV) (33.80 %), indicating that significant extensive demethylation had occurred. However, the non-heterotic hybrids displayed a DNA methylation level (33.59 %) that was close to the MPV, and they contained similar numbers of methylation and demethylation events. The correlation analysis revealed that the increased gene expression in the heterotic hybrids was associated with its overall low genomic DNA methylation level and dynamic DNA methylation patterns. Twenty fragments with adjusted DNA methylation patterns were then cloned and the transcript level analyzed quantitatively. The results further indicated that the expressions of most but not all of the 12 possible genes were negatively correlated to their respective DNA methylation status. Based on these data, we suggest that adjustments in DNA methylation status may play critical roles in the formation of heterosis produced by the cross between two Larix kaempferi intraspecific parental lines. |
| abstractGer |
Abstract Heterosis is prevalent in Larix kaempferi, but the molecular mechanism underlying heterosis in this species has not yet been elucidated. We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Larix kaempferi intraspecific parental lines and their reciprocal hybrids. The results indicate that the two intraspecific parental lines have highly similar genome structures and that the crosses did not produce substantial changes in the genomic DNA of the hybrid progeny. In contrast, there was a significant difference in the DNA methylation status between the two parental lines. The genomic DNA methylation level in the heterotic hybrids (26.47 %) was significantly lower than the midparent value (MPV) (33.80 %), indicating that significant extensive demethylation had occurred. However, the non-heterotic hybrids displayed a DNA methylation level (33.59 %) that was close to the MPV, and they contained similar numbers of methylation and demethylation events. The correlation analysis revealed that the increased gene expression in the heterotic hybrids was associated with its overall low genomic DNA methylation level and dynamic DNA methylation patterns. Twenty fragments with adjusted DNA methylation patterns were then cloned and the transcript level analyzed quantitatively. The results further indicated that the expressions of most but not all of the 12 possible genes were negatively correlated to their respective DNA methylation status. Based on these data, we suggest that adjustments in DNA methylation status may play critical roles in the formation of heterosis produced by the cross between two Larix kaempferi intraspecific parental lines. |
| abstract_unstemmed |
Abstract Heterosis is prevalent in Larix kaempferi, but the molecular mechanism underlying heterosis in this species has not yet been elucidated. We have explored the relationships among heterosis and DNA sequence variations, changes in the DNA methylation status, and gene expression in a set of Larix kaempferi intraspecific parental lines and their reciprocal hybrids. The results indicate that the two intraspecific parental lines have highly similar genome structures and that the crosses did not produce substantial changes in the genomic DNA of the hybrid progeny. In contrast, there was a significant difference in the DNA methylation status between the two parental lines. The genomic DNA methylation level in the heterotic hybrids (26.47 %) was significantly lower than the midparent value (MPV) (33.80 %), indicating that significant extensive demethylation had occurred. However, the non-heterotic hybrids displayed a DNA methylation level (33.59 %) that was close to the MPV, and they contained similar numbers of methylation and demethylation events. The correlation analysis revealed that the increased gene expression in the heterotic hybrids was associated with its overall low genomic DNA methylation level and dynamic DNA methylation patterns. Twenty fragments with adjusted DNA methylation patterns were then cloned and the transcript level analyzed quantitatively. The results further indicated that the expressions of most but not all of the 12 possible genes were negatively correlated to their respective DNA methylation status. Based on these data, we suggest that adjustments in DNA methylation status may play critical roles in the formation of heterosis produced by the cross between two Larix kaempferi intraspecific parental lines. |
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| container_issue |
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| title_short |
DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids |
| url |
https://dx.doi.org/10.1007/s11032-012-9803-y |
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Song, Wen-Qin Chen, Cheng-Bin Zhou, Ya-Nan Qi, Li-Wang Wang, Chun-Guo |
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Song, Wen-Qin Chen, Cheng-Bin Zhou, Ya-Nan Qi, Li-Wang Wang, Chun-Guo |
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| doi_str |
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| up_date |
2024-07-03T19:00:44.431Z |
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| score |
7.401063 |