Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli
Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Xue, Ren Feng [verfasserIn] Wu, Jing [verfasserIn] Chen, Ming Li [verfasserIn] Zhu, Zhen Dong [verfasserIn] Wang, Lan Fen [verfasserIn] Wang, Xiao Ming [verfasserIn] Blair, Matthew W. [verfasserIn] Wang, Shu Min [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Molecular breeding - Dordrecht : Springer Science + Business Media B.V., 1995, 34(2014), 3 vom: 13. Mai, Seite 855-870 |
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| Übergeordnetes Werk: |
volume:34 ; year:2014 ; number:3 ; day:13 ; month:05 ; pages:855-870 |
| Links: |
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| DOI / URN: |
10.1007/s11032-014-0080-9 |
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| Katalog-ID: |
SPR015855015 |
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| 100 | 1 | |a Xue, Ren Feng |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli |
| 264 | 1 | |c 2014 | |
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| 520 | |a Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli infection. Rapid amplification of cDNA ends was used to clone the full gene. The source of the differentially expressed fragment was the resistant genotype CAAS260205. The common bean POX gene showed a similar gene structure to a soybean POX gene consisting of four exons and three introns. Sequence analysis showed that PvPOX1 contained two conserved functional domains: a peroxidae active enzymatic site and a peroxidase heme-ligand site in close proximity. Phylogenetic study differentiated the new PvPOX1 gene from previous peroxidases in this multi-gene family. Real-time PCR analyses indicated that expression of the PvPOX1 gene was upregulated by Fusarium infection and that peroxidase activity, as measured by hydrogen peroxide ($ H_{2} %$ O_{2} $) accumulation, was enhanced during disease. The mRNA transcript level of PvPOX1 was also significantly increased following the stresses of wounding and polyethylene glycol, salicylic acid and abscisic acid treatments. The results of subcellular localization studies indicated that the localization of the PvPOX1 protein was in the apoplastic region of root cells. A co-dominant marker was developed for genetic mapping of the PvPOX1 gene based on an indel/insertion between the sequences of Andean and Mesoamerican parental genotypes (Hongyundou and Jingdou), which are mapping parents used in resistance breeding. Based on the new genetic marker, the gene was located between simple sequence repeat markers BMg2113 and BM181 on chromosome 3 of common bean and was not related to other peroxidases in terms of location. Overall, these results facilitate understanding of the molecular mechanism controlling resistance to Fusarium wilt pathogens and provide a diagnostic marker for selection of resistance to this disease based on peroxidase expression in common bean. | ||
| 650 | 4 | |a cDNA-AFLP analysis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a gene |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Expression characterization |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Subcellular localization |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Genetic mapping |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Wu, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Ming Li |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Zhen Dong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Lan Fen |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiao Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Blair, Matthew W. |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shu Min |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular breeding |d Dordrecht : Springer Science + Business Media B.V., 1995 |g 34(2014), 3 vom: 13. Mai, Seite 855-870 |w (DE-627)270930671 |w (DE-600)1478220-0 |x 1572-9788 |7 nnns |
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10.1007/s11032-014-0080-9 doi (DE-627)SPR015855015 (SPR)s11032-014-0080-9-e DE-627 ger DE-627 rakwb eng 580 ASE 48.58 bkl 42.43 bkl Xue, Ren Feng verfasserin aut Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli infection. Rapid amplification of cDNA ends was used to clone the full gene. The source of the differentially expressed fragment was the resistant genotype CAAS260205. The common bean POX gene showed a similar gene structure to a soybean POX gene consisting of four exons and three introns. Sequence analysis showed that PvPOX1 contained two conserved functional domains: a peroxidae active enzymatic site and a peroxidase heme-ligand site in close proximity. Phylogenetic study differentiated the new PvPOX1 gene from previous peroxidases in this multi-gene family. Real-time PCR analyses indicated that expression of the PvPOX1 gene was upregulated by Fusarium infection and that peroxidase activity, as measured by hydrogen peroxide ($ H_{2} %$ O_{2} $) accumulation, was enhanced during disease. The mRNA transcript level of PvPOX1 was also significantly increased following the stresses of wounding and polyethylene glycol, salicylic acid and abscisic acid treatments. The results of subcellular localization studies indicated that the localization of the PvPOX1 protein was in the apoplastic region of root cells. A co-dominant marker was developed for genetic mapping of the PvPOX1 gene based on an indel/insertion between the sequences of Andean and Mesoamerican parental genotypes (Hongyundou and Jingdou), which are mapping parents used in resistance breeding. Based on the new genetic marker, the gene was located between simple sequence repeat markers BMg2113 and BM181 on chromosome 3 of common bean and was not related to other peroxidases in terms of location. Overall, these results facilitate understanding of the molecular mechanism controlling resistance to Fusarium wilt pathogens and provide a diagnostic marker for selection of resistance to this disease based on peroxidase expression in common bean. cDNA-AFLP analysis (dpeaa)DE-He213 gene (dpeaa)DE-He213 Expression characterization (dpeaa)DE-He213 Subcellular localization (dpeaa)DE-He213 Genetic mapping (dpeaa)DE-He213 Wu, Jing verfasserin aut Chen, Ming Li verfasserin aut Zhu, Zhen Dong verfasserin aut Wang, Lan Fen verfasserin aut Wang, Xiao Ming verfasserin aut Blair, Matthew W. verfasserin aut Wang, Shu Min verfasserin aut Enthalten in Molecular breeding Dordrecht : Springer Science + Business Media B.V., 1995 34(2014), 3 vom: 13. Mai, Seite 855-870 (DE-627)270930671 (DE-600)1478220-0 1572-9788 nnns volume:34 year:2014 number:3 day:13 month:05 pages:855-870 https://dx.doi.org/10.1007/s11032-014-0080-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.58 ASE 42.43 ASE AR 34 2014 3 13 05 855-870 |
| spelling |
10.1007/s11032-014-0080-9 doi (DE-627)SPR015855015 (SPR)s11032-014-0080-9-e DE-627 ger DE-627 rakwb eng 580 ASE 48.58 bkl 42.43 bkl Xue, Ren Feng verfasserin aut Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli infection. Rapid amplification of cDNA ends was used to clone the full gene. The source of the differentially expressed fragment was the resistant genotype CAAS260205. The common bean POX gene showed a similar gene structure to a soybean POX gene consisting of four exons and three introns. Sequence analysis showed that PvPOX1 contained two conserved functional domains: a peroxidae active enzymatic site and a peroxidase heme-ligand site in close proximity. Phylogenetic study differentiated the new PvPOX1 gene from previous peroxidases in this multi-gene family. Real-time PCR analyses indicated that expression of the PvPOX1 gene was upregulated by Fusarium infection and that peroxidase activity, as measured by hydrogen peroxide ($ H_{2} %$ O_{2} $) accumulation, was enhanced during disease. The mRNA transcript level of PvPOX1 was also significantly increased following the stresses of wounding and polyethylene glycol, salicylic acid and abscisic acid treatments. The results of subcellular localization studies indicated that the localization of the PvPOX1 protein was in the apoplastic region of root cells. A co-dominant marker was developed for genetic mapping of the PvPOX1 gene based on an indel/insertion between the sequences of Andean and Mesoamerican parental genotypes (Hongyundou and Jingdou), which are mapping parents used in resistance breeding. Based on the new genetic marker, the gene was located between simple sequence repeat markers BMg2113 and BM181 on chromosome 3 of common bean and was not related to other peroxidases in terms of location. Overall, these results facilitate understanding of the molecular mechanism controlling resistance to Fusarium wilt pathogens and provide a diagnostic marker for selection of resistance to this disease based on peroxidase expression in common bean. cDNA-AFLP analysis (dpeaa)DE-He213 gene (dpeaa)DE-He213 Expression characterization (dpeaa)DE-He213 Subcellular localization (dpeaa)DE-He213 Genetic mapping (dpeaa)DE-He213 Wu, Jing verfasserin aut Chen, Ming Li verfasserin aut Zhu, Zhen Dong verfasserin aut Wang, Lan Fen verfasserin aut Wang, Xiao Ming verfasserin aut Blair, Matthew W. verfasserin aut Wang, Shu Min verfasserin aut Enthalten in Molecular breeding Dordrecht : Springer Science + Business Media B.V., 1995 34(2014), 3 vom: 13. Mai, Seite 855-870 (DE-627)270930671 (DE-600)1478220-0 1572-9788 nnns volume:34 year:2014 number:3 day:13 month:05 pages:855-870 https://dx.doi.org/10.1007/s11032-014-0080-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.58 ASE 42.43 ASE AR 34 2014 3 13 05 855-870 |
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10.1007/s11032-014-0080-9 doi (DE-627)SPR015855015 (SPR)s11032-014-0080-9-e DE-627 ger DE-627 rakwb eng 580 ASE 48.58 bkl 42.43 bkl Xue, Ren Feng verfasserin aut Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli infection. Rapid amplification of cDNA ends was used to clone the full gene. The source of the differentially expressed fragment was the resistant genotype CAAS260205. The common bean POX gene showed a similar gene structure to a soybean POX gene consisting of four exons and three introns. Sequence analysis showed that PvPOX1 contained two conserved functional domains: a peroxidae active enzymatic site and a peroxidase heme-ligand site in close proximity. Phylogenetic study differentiated the new PvPOX1 gene from previous peroxidases in this multi-gene family. Real-time PCR analyses indicated that expression of the PvPOX1 gene was upregulated by Fusarium infection and that peroxidase activity, as measured by hydrogen peroxide ($ H_{2} %$ O_{2} $) accumulation, was enhanced during disease. The mRNA transcript level of PvPOX1 was also significantly increased following the stresses of wounding and polyethylene glycol, salicylic acid and abscisic acid treatments. The results of subcellular localization studies indicated that the localization of the PvPOX1 protein was in the apoplastic region of root cells. A co-dominant marker was developed for genetic mapping of the PvPOX1 gene based on an indel/insertion between the sequences of Andean and Mesoamerican parental genotypes (Hongyundou and Jingdou), which are mapping parents used in resistance breeding. Based on the new genetic marker, the gene was located between simple sequence repeat markers BMg2113 and BM181 on chromosome 3 of common bean and was not related to other peroxidases in terms of location. Overall, these results facilitate understanding of the molecular mechanism controlling resistance to Fusarium wilt pathogens and provide a diagnostic marker for selection of resistance to this disease based on peroxidase expression in common bean. cDNA-AFLP analysis (dpeaa)DE-He213 gene (dpeaa)DE-He213 Expression characterization (dpeaa)DE-He213 Subcellular localization (dpeaa)DE-He213 Genetic mapping (dpeaa)DE-He213 Wu, Jing verfasserin aut Chen, Ming Li verfasserin aut Zhu, Zhen Dong verfasserin aut Wang, Lan Fen verfasserin aut Wang, Xiao Ming verfasserin aut Blair, Matthew W. verfasserin aut Wang, Shu Min verfasserin aut Enthalten in Molecular breeding Dordrecht : Springer Science + Business Media B.V., 1995 34(2014), 3 vom: 13. Mai, Seite 855-870 (DE-627)270930671 (DE-600)1478220-0 1572-9788 nnns volume:34 year:2014 number:3 day:13 month:05 pages:855-870 https://dx.doi.org/10.1007/s11032-014-0080-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.58 ASE 42.43 ASE AR 34 2014 3 13 05 855-870 |
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10.1007/s11032-014-0080-9 doi (DE-627)SPR015855015 (SPR)s11032-014-0080-9-e DE-627 ger DE-627 rakwb eng 580 ASE 48.58 bkl 42.43 bkl Xue, Ren Feng verfasserin aut Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli infection. Rapid amplification of cDNA ends was used to clone the full gene. The source of the differentially expressed fragment was the resistant genotype CAAS260205. The common bean POX gene showed a similar gene structure to a soybean POX gene consisting of four exons and three introns. Sequence analysis showed that PvPOX1 contained two conserved functional domains: a peroxidae active enzymatic site and a peroxidase heme-ligand site in close proximity. Phylogenetic study differentiated the new PvPOX1 gene from previous peroxidases in this multi-gene family. Real-time PCR analyses indicated that expression of the PvPOX1 gene was upregulated by Fusarium infection and that peroxidase activity, as measured by hydrogen peroxide ($ H_{2} %$ O_{2} $) accumulation, was enhanced during disease. The mRNA transcript level of PvPOX1 was also significantly increased following the stresses of wounding and polyethylene glycol, salicylic acid and abscisic acid treatments. The results of subcellular localization studies indicated that the localization of the PvPOX1 protein was in the apoplastic region of root cells. A co-dominant marker was developed for genetic mapping of the PvPOX1 gene based on an indel/insertion between the sequences of Andean and Mesoamerican parental genotypes (Hongyundou and Jingdou), which are mapping parents used in resistance breeding. Based on the new genetic marker, the gene was located between simple sequence repeat markers BMg2113 and BM181 on chromosome 3 of common bean and was not related to other peroxidases in terms of location. Overall, these results facilitate understanding of the molecular mechanism controlling resistance to Fusarium wilt pathogens and provide a diagnostic marker for selection of resistance to this disease based on peroxidase expression in common bean. cDNA-AFLP analysis (dpeaa)DE-He213 gene (dpeaa)DE-He213 Expression characterization (dpeaa)DE-He213 Subcellular localization (dpeaa)DE-He213 Genetic mapping (dpeaa)DE-He213 Wu, Jing verfasserin aut Chen, Ming Li verfasserin aut Zhu, Zhen Dong verfasserin aut Wang, Lan Fen verfasserin aut Wang, Xiao Ming verfasserin aut Blair, Matthew W. verfasserin aut Wang, Shu Min verfasserin aut Enthalten in Molecular breeding Dordrecht : Springer Science + Business Media B.V., 1995 34(2014), 3 vom: 13. Mai, Seite 855-870 (DE-627)270930671 (DE-600)1478220-0 1572-9788 nnns volume:34 year:2014 number:3 day:13 month:05 pages:855-870 https://dx.doi.org/10.1007/s11032-014-0080-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.58 ASE 42.43 ASE AR 34 2014 3 13 05 855-870 |
| allfieldsSound |
10.1007/s11032-014-0080-9 doi (DE-627)SPR015855015 (SPR)s11032-014-0080-9-e DE-627 ger DE-627 rakwb eng 580 ASE 48.58 bkl 42.43 bkl Xue, Ren Feng verfasserin aut Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli infection. Rapid amplification of cDNA ends was used to clone the full gene. The source of the differentially expressed fragment was the resistant genotype CAAS260205. The common bean POX gene showed a similar gene structure to a soybean POX gene consisting of four exons and three introns. Sequence analysis showed that PvPOX1 contained two conserved functional domains: a peroxidae active enzymatic site and a peroxidase heme-ligand site in close proximity. Phylogenetic study differentiated the new PvPOX1 gene from previous peroxidases in this multi-gene family. Real-time PCR analyses indicated that expression of the PvPOX1 gene was upregulated by Fusarium infection and that peroxidase activity, as measured by hydrogen peroxide ($ H_{2} %$ O_{2} $) accumulation, was enhanced during disease. The mRNA transcript level of PvPOX1 was also significantly increased following the stresses of wounding and polyethylene glycol, salicylic acid and abscisic acid treatments. The results of subcellular localization studies indicated that the localization of the PvPOX1 protein was in the apoplastic region of root cells. A co-dominant marker was developed for genetic mapping of the PvPOX1 gene based on an indel/insertion between the sequences of Andean and Mesoamerican parental genotypes (Hongyundou and Jingdou), which are mapping parents used in resistance breeding. Based on the new genetic marker, the gene was located between simple sequence repeat markers BMg2113 and BM181 on chromosome 3 of common bean and was not related to other peroxidases in terms of location. Overall, these results facilitate understanding of the molecular mechanism controlling resistance to Fusarium wilt pathogens and provide a diagnostic marker for selection of resistance to this disease based on peroxidase expression in common bean. cDNA-AFLP analysis (dpeaa)DE-He213 gene (dpeaa)DE-He213 Expression characterization (dpeaa)DE-He213 Subcellular localization (dpeaa)DE-He213 Genetic mapping (dpeaa)DE-He213 Wu, Jing verfasserin aut Chen, Ming Li verfasserin aut Zhu, Zhen Dong verfasserin aut Wang, Lan Fen verfasserin aut Wang, Xiao Ming verfasserin aut Blair, Matthew W. verfasserin aut Wang, Shu Min verfasserin aut Enthalten in Molecular breeding Dordrecht : Springer Science + Business Media B.V., 1995 34(2014), 3 vom: 13. Mai, Seite 855-870 (DE-627)270930671 (DE-600)1478220-0 1572-9788 nnns volume:34 year:2014 number:3 day:13 month:05 pages:855-870 https://dx.doi.org/10.1007/s11032-014-0080-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 48.58 ASE 42.43 ASE AR 34 2014 3 13 05 855-870 |
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Enthalten in Molecular breeding 34(2014), 3 vom: 13. Mai, Seite 855-870 volume:34 year:2014 number:3 day:13 month:05 pages:855-870 |
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Enthalten in Molecular breeding 34(2014), 3 vom: 13. Mai, Seite 855-870 volume:34 year:2014 number:3 day:13 month:05 pages:855-870 |
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cDNA-AFLP analysis gene Expression characterization Subcellular localization Genetic mapping |
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Xue, Ren Feng @@aut@@ Wu, Jing @@aut@@ Chen, Ming Li @@aut@@ Zhu, Zhen Dong @@aut@@ Wang, Lan Fen @@aut@@ Wang, Xiao Ming @@aut@@ Blair, Matthew W. @@aut@@ Wang, Shu Min @@aut@@ |
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Phaseoli</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2014</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli infection. Rapid amplification of cDNA ends was used to clone the full gene. The source of the differentially expressed fragment was the resistant genotype CAAS260205. The common bean POX gene showed a similar gene structure to a soybean POX gene consisting of four exons and three introns. Sequence analysis showed that PvPOX1 contained two conserved functional domains: a peroxidae active enzymatic site and a peroxidase heme-ligand site in close proximity. Phylogenetic study differentiated the new PvPOX1 gene from previous peroxidases in this multi-gene family. Real-time PCR analyses indicated that expression of the PvPOX1 gene was upregulated by Fusarium infection and that peroxidase activity, as measured by hydrogen peroxide ($ H_{2} %$ O_{2} $) accumulation, was enhanced during disease. The mRNA transcript level of PvPOX1 was also significantly increased following the stresses of wounding and polyethylene glycol, salicylic acid and abscisic acid treatments. The results of subcellular localization studies indicated that the localization of the PvPOX1 protein was in the apoplastic region of root cells. A co-dominant marker was developed for genetic mapping of the PvPOX1 gene based on an indel/insertion between the sequences of Andean and Mesoamerican parental genotypes (Hongyundou and Jingdou), which are mapping parents used in resistance breeding. Based on the new genetic marker, the gene was located between simple sequence repeat markers BMg2113 and BM181 on chromosome 3 of common bean and was not related to other peroxidases in terms of location. 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Xue, Ren Feng |
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Xue, Ren Feng ddc 580 bkl 48.58 bkl 42.43 misc cDNA-AFLP analysis misc gene misc Expression characterization misc Subcellular localization misc Genetic mapping Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli |
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580 ASE 48.58 bkl 42.43 bkl Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli cDNA-AFLP analysis (dpeaa)DE-He213 gene (dpeaa)DE-He213 Expression characterization (dpeaa)DE-He213 Subcellular localization (dpeaa)DE-He213 Genetic mapping (dpeaa)DE-He213 |
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ddc 580 bkl 48.58 bkl 42.43 misc cDNA-AFLP analysis misc gene misc Expression characterization misc Subcellular localization misc Genetic mapping |
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ddc 580 bkl 48.58 bkl 42.43 misc cDNA-AFLP analysis misc gene misc Expression characterization misc Subcellular localization misc Genetic mapping |
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ddc 580 bkl 48.58 bkl 42.43 misc cDNA-AFLP analysis misc gene misc Expression characterization misc Subcellular localization misc Genetic mapping |
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Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli |
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(DE-627)SPR015855015 (SPR)s11032-014-0080-9-e |
| title_full |
Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli |
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Xue, Ren Feng |
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Molecular breeding |
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Molecular breeding |
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eng |
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500 - Science |
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2014 |
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855 |
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Xue, Ren Feng Wu, Jing Chen, Ming Li Zhu, Zhen Dong Wang, Lan Fen Wang, Xiao Ming Blair, Matthew W. Wang, Shu Min |
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34 |
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580 ASE 48.58 bkl 42.43 bkl |
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Elektronische Aufsätze |
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Xue, Ren Feng |
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10.1007/s11032-014-0080-9 |
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580 |
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verfasserin |
| title_sort |
cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (phaseolus vulgaris l.) infected with fusarium oxysporum f. sp. phaseoli |
| title_auth |
Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli |
| abstract |
Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli infection. Rapid amplification of cDNA ends was used to clone the full gene. The source of the differentially expressed fragment was the resistant genotype CAAS260205. The common bean POX gene showed a similar gene structure to a soybean POX gene consisting of four exons and three introns. Sequence analysis showed that PvPOX1 contained two conserved functional domains: a peroxidae active enzymatic site and a peroxidase heme-ligand site in close proximity. Phylogenetic study differentiated the new PvPOX1 gene from previous peroxidases in this multi-gene family. Real-time PCR analyses indicated that expression of the PvPOX1 gene was upregulated by Fusarium infection and that peroxidase activity, as measured by hydrogen peroxide ($ H_{2} %$ O_{2} $) accumulation, was enhanced during disease. The mRNA transcript level of PvPOX1 was also significantly increased following the stresses of wounding and polyethylene glycol, salicylic acid and abscisic acid treatments. The results of subcellular localization studies indicated that the localization of the PvPOX1 protein was in the apoplastic region of root cells. A co-dominant marker was developed for genetic mapping of the PvPOX1 gene based on an indel/insertion between the sequences of Andean and Mesoamerican parental genotypes (Hongyundou and Jingdou), which are mapping parents used in resistance breeding. Based on the new genetic marker, the gene was located between simple sequence repeat markers BMg2113 and BM181 on chromosome 3 of common bean and was not related to other peroxidases in terms of location. Overall, these results facilitate understanding of the molecular mechanism controlling resistance to Fusarium wilt pathogens and provide a diagnostic marker for selection of resistance to this disease based on peroxidase expression in common bean. |
| abstractGer |
Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli infection. Rapid amplification of cDNA ends was used to clone the full gene. The source of the differentially expressed fragment was the resistant genotype CAAS260205. The common bean POX gene showed a similar gene structure to a soybean POX gene consisting of four exons and three introns. Sequence analysis showed that PvPOX1 contained two conserved functional domains: a peroxidae active enzymatic site and a peroxidase heme-ligand site in close proximity. Phylogenetic study differentiated the new PvPOX1 gene from previous peroxidases in this multi-gene family. Real-time PCR analyses indicated that expression of the PvPOX1 gene was upregulated by Fusarium infection and that peroxidase activity, as measured by hydrogen peroxide ($ H_{2} %$ O_{2} $) accumulation, was enhanced during disease. The mRNA transcript level of PvPOX1 was also significantly increased following the stresses of wounding and polyethylene glycol, salicylic acid and abscisic acid treatments. The results of subcellular localization studies indicated that the localization of the PvPOX1 protein was in the apoplastic region of root cells. A co-dominant marker was developed for genetic mapping of the PvPOX1 gene based on an indel/insertion between the sequences of Andean and Mesoamerican parental genotypes (Hongyundou and Jingdou), which are mapping parents used in resistance breeding. Based on the new genetic marker, the gene was located between simple sequence repeat markers BMg2113 and BM181 on chromosome 3 of common bean and was not related to other peroxidases in terms of location. Overall, these results facilitate understanding of the molecular mechanism controlling resistance to Fusarium wilt pathogens and provide a diagnostic marker for selection of resistance to this disease based on peroxidase expression in common bean. |
| abstract_unstemmed |
Abstract Peroxidases play an important role in plant defense response to the attack of a host tissue by many pathogens. In this study, a new POX gene was isolated from common bean roots using cDNA-AFLP libraries and differential display of tissues with and without Fusarium oxysporum f. sp. phaseoli infection. Rapid amplification of cDNA ends was used to clone the full gene. The source of the differentially expressed fragment was the resistant genotype CAAS260205. The common bean POX gene showed a similar gene structure to a soybean POX gene consisting of four exons and three introns. Sequence analysis showed that PvPOX1 contained two conserved functional domains: a peroxidae active enzymatic site and a peroxidase heme-ligand site in close proximity. Phylogenetic study differentiated the new PvPOX1 gene from previous peroxidases in this multi-gene family. Real-time PCR analyses indicated that expression of the PvPOX1 gene was upregulated by Fusarium infection and that peroxidase activity, as measured by hydrogen peroxide ($ H_{2} %$ O_{2} $) accumulation, was enhanced during disease. The mRNA transcript level of PvPOX1 was also significantly increased following the stresses of wounding and polyethylene glycol, salicylic acid and abscisic acid treatments. The results of subcellular localization studies indicated that the localization of the PvPOX1 protein was in the apoplastic region of root cells. A co-dominant marker was developed for genetic mapping of the PvPOX1 gene based on an indel/insertion between the sequences of Andean and Mesoamerican parental genotypes (Hongyundou and Jingdou), which are mapping parents used in resistance breeding. Based on the new genetic marker, the gene was located between simple sequence repeat markers BMg2113 and BM181 on chromosome 3 of common bean and was not related to other peroxidases in terms of location. Overall, these results facilitate understanding of the molecular mechanism controlling resistance to Fusarium wilt pathogens and provide a diagnostic marker for selection of resistance to this disease based on peroxidase expression in common bean. |
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3 |
| title_short |
Cloning and characterization of a novel secretory root-expressed peroxidase gene from common bean (Phaseolus vulgaris L.) infected with Fusarium oxysporum f. sp. Phaseoli |
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https://dx.doi.org/10.1007/s11032-014-0080-9 |
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Wu, Jing Chen, Ming Li Zhu, Zhen Dong Wang, Lan Fen Wang, Xiao Ming Blair, Matthew W. Wang, Shu Min |
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| score |
7.4029083 |