Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods
Abstract This multicenter study assesses the value of $ O^{6} $-methylguanine-DNA methyltransferase (MGMT) status for predicting overall survival in glioblastoma patients. Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients wi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Karayan-Tapon, Lucie [verfasserIn] Quillien, Véronique [verfasserIn] Guilhot, Joëlle [verfasserIn] Wager, Michel [verfasserIn] Fromont, Gaëlle [verfasserIn] Saikali, Stephan [verfasserIn] Etcheverry, Amandine [verfasserIn] Hamlat, Abderrahmane [verfasserIn] Loussouarn, Delphine [verfasserIn] Campion, Loïc [verfasserIn] Campone, Mario [verfasserIn] Vallette, François-Marie [verfasserIn] Gratas-Rabbia-Ré, Catherine [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of neuro-oncology - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1983, 97(2009), 3 vom: 20. Okt., Seite 311-322 |
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| Übergeordnetes Werk: |
volume:97 ; year:2009 ; number:3 ; day:20 ; month:10 ; pages:311-322 |
| Links: |
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| DOI / URN: |
10.1007/s11060-009-0031-1 |
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| Katalog-ID: |
SPR016165551 |
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| 245 | 1 | 0 | |a Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods |
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| 520 | |a Abstract This multicenter study assesses the value of $ O^{6} $-methylguanine-DNA methyltransferase (MGMT) status for predicting overall survival in glioblastoma patients. Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients with glioblastomas treated by surgery and radiotherapy with concomitant temozolomide (TMZ) followed by adjuvant TMZ. MGMT promoter methylation was assessed by qualitative methyl-specific polymerase chain reaction (MSP), semiquantitative methyl-specific polymerase chain reaction (SQ-MSP), and pyrosequencing, while MGMT expression was measured at the RNA level by quantitative real-time PCR (Q-RT-PCR) and at the protein level by immunohistochemistry (IHC). MGMT promoter methylation as evaluated by MSP, SQ-MSP, and pyrosequencing was significantly correlated with overall survival. The best predictive value was obtained by pyrosequencing of one specific CpG position. Overall survival was 14 and 25 months for patients with percentages of methylation below and above the median, respectively. In contrast, MGMT status determined by Q-RT-PCR and IHC showed little or no correlation with overall survival, respectively. These results confirm the prognostic value of MGMT promoter methylation in glioblastoma patients initially treated with TMZ. SQ-MSP allowed better discrimination than classical MSP, and pyrosequencing represented a good option. | ||
| 650 | 4 | |a MGMT methylation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Temozolomide |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Glioblastoma |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Overall survival |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Quillien, Véronique |e verfasserin |4 aut | |
| 700 | 1 | |a Guilhot, Joëlle |e verfasserin |4 aut | |
| 700 | 1 | |a Wager, Michel |e verfasserin |4 aut | |
| 700 | 1 | |a Fromont, Gaëlle |e verfasserin |4 aut | |
| 700 | 1 | |a Saikali, Stephan |e verfasserin |4 aut | |
| 700 | 1 | |a Etcheverry, Amandine |e verfasserin |4 aut | |
| 700 | 1 | |a Hamlat, Abderrahmane |e verfasserin |4 aut | |
| 700 | 1 | |a Loussouarn, Delphine |e verfasserin |4 aut | |
| 700 | 1 | |a Campion, Loïc |e verfasserin |4 aut | |
| 700 | 1 | |a Campone, Mario |e verfasserin |4 aut | |
| 700 | 1 | |a Vallette, François-Marie |e verfasserin |4 aut | |
| 700 | 1 | |a Gratas-Rabbia-Ré, Catherine |e verfasserin |4 aut | |
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10.1007/s11060-009-0031-1 doi (DE-627)SPR016165551 (SPR)s11060-009-0031-1-e DE-627 ger DE-627 rakwb eng 610 ASE 44.81 bkl 44.90 bkl Karayan-Tapon, Lucie verfasserin aut Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This multicenter study assesses the value of $ O^{6} $-methylguanine-DNA methyltransferase (MGMT) status for predicting overall survival in glioblastoma patients. Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients with glioblastomas treated by surgery and radiotherapy with concomitant temozolomide (TMZ) followed by adjuvant TMZ. MGMT promoter methylation was assessed by qualitative methyl-specific polymerase chain reaction (MSP), semiquantitative methyl-specific polymerase chain reaction (SQ-MSP), and pyrosequencing, while MGMT expression was measured at the RNA level by quantitative real-time PCR (Q-RT-PCR) and at the protein level by immunohistochemistry (IHC). MGMT promoter methylation as evaluated by MSP, SQ-MSP, and pyrosequencing was significantly correlated with overall survival. The best predictive value was obtained by pyrosequencing of one specific CpG position. Overall survival was 14 and 25 months for patients with percentages of methylation below and above the median, respectively. In contrast, MGMT status determined by Q-RT-PCR and IHC showed little or no correlation with overall survival, respectively. These results confirm the prognostic value of MGMT promoter methylation in glioblastoma patients initially treated with TMZ. SQ-MSP allowed better discrimination than classical MSP, and pyrosequencing represented a good option. MGMT methylation (dpeaa)DE-He213 Temozolomide (dpeaa)DE-He213 Glioblastoma (dpeaa)DE-He213 Overall survival (dpeaa)DE-He213 Quillien, Véronique verfasserin aut Guilhot, Joëlle verfasserin aut Wager, Michel verfasserin aut Fromont, Gaëlle verfasserin aut Saikali, Stephan verfasserin aut Etcheverry, Amandine verfasserin aut Hamlat, Abderrahmane verfasserin aut Loussouarn, Delphine verfasserin aut Campion, Loïc verfasserin aut Campone, Mario verfasserin aut Vallette, François-Marie verfasserin aut Gratas-Rabbia-Ré, Catherine verfasserin aut Enthalten in Journal of neuro-oncology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1983 97(2009), 3 vom: 20. Okt., Seite 311-322 (DE-627)32046122X (DE-600)2007293-4 1573-7373 nnns volume:97 year:2009 number:3 day:20 month:10 pages:311-322 https://dx.doi.org/10.1007/s11060-009-0031-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.81 ASE 44.90 ASE AR 97 2009 3 20 10 311-322 |
| spelling |
10.1007/s11060-009-0031-1 doi (DE-627)SPR016165551 (SPR)s11060-009-0031-1-e DE-627 ger DE-627 rakwb eng 610 ASE 44.81 bkl 44.90 bkl Karayan-Tapon, Lucie verfasserin aut Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This multicenter study assesses the value of $ O^{6} $-methylguanine-DNA methyltransferase (MGMT) status for predicting overall survival in glioblastoma patients. Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients with glioblastomas treated by surgery and radiotherapy with concomitant temozolomide (TMZ) followed by adjuvant TMZ. MGMT promoter methylation was assessed by qualitative methyl-specific polymerase chain reaction (MSP), semiquantitative methyl-specific polymerase chain reaction (SQ-MSP), and pyrosequencing, while MGMT expression was measured at the RNA level by quantitative real-time PCR (Q-RT-PCR) and at the protein level by immunohistochemistry (IHC). MGMT promoter methylation as evaluated by MSP, SQ-MSP, and pyrosequencing was significantly correlated with overall survival. The best predictive value was obtained by pyrosequencing of one specific CpG position. Overall survival was 14 and 25 months for patients with percentages of methylation below and above the median, respectively. In contrast, MGMT status determined by Q-RT-PCR and IHC showed little or no correlation with overall survival, respectively. These results confirm the prognostic value of MGMT promoter methylation in glioblastoma patients initially treated with TMZ. SQ-MSP allowed better discrimination than classical MSP, and pyrosequencing represented a good option. MGMT methylation (dpeaa)DE-He213 Temozolomide (dpeaa)DE-He213 Glioblastoma (dpeaa)DE-He213 Overall survival (dpeaa)DE-He213 Quillien, Véronique verfasserin aut Guilhot, Joëlle verfasserin aut Wager, Michel verfasserin aut Fromont, Gaëlle verfasserin aut Saikali, Stephan verfasserin aut Etcheverry, Amandine verfasserin aut Hamlat, Abderrahmane verfasserin aut Loussouarn, Delphine verfasserin aut Campion, Loïc verfasserin aut Campone, Mario verfasserin aut Vallette, François-Marie verfasserin aut Gratas-Rabbia-Ré, Catherine verfasserin aut Enthalten in Journal of neuro-oncology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1983 97(2009), 3 vom: 20. Okt., Seite 311-322 (DE-627)32046122X (DE-600)2007293-4 1573-7373 nnns volume:97 year:2009 number:3 day:20 month:10 pages:311-322 https://dx.doi.org/10.1007/s11060-009-0031-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.81 ASE 44.90 ASE AR 97 2009 3 20 10 311-322 |
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10.1007/s11060-009-0031-1 doi (DE-627)SPR016165551 (SPR)s11060-009-0031-1-e DE-627 ger DE-627 rakwb eng 610 ASE 44.81 bkl 44.90 bkl Karayan-Tapon, Lucie verfasserin aut Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This multicenter study assesses the value of $ O^{6} $-methylguanine-DNA methyltransferase (MGMT) status for predicting overall survival in glioblastoma patients. Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients with glioblastomas treated by surgery and radiotherapy with concomitant temozolomide (TMZ) followed by adjuvant TMZ. MGMT promoter methylation was assessed by qualitative methyl-specific polymerase chain reaction (MSP), semiquantitative methyl-specific polymerase chain reaction (SQ-MSP), and pyrosequencing, while MGMT expression was measured at the RNA level by quantitative real-time PCR (Q-RT-PCR) and at the protein level by immunohistochemistry (IHC). MGMT promoter methylation as evaluated by MSP, SQ-MSP, and pyrosequencing was significantly correlated with overall survival. The best predictive value was obtained by pyrosequencing of one specific CpG position. Overall survival was 14 and 25 months for patients with percentages of methylation below and above the median, respectively. In contrast, MGMT status determined by Q-RT-PCR and IHC showed little or no correlation with overall survival, respectively. These results confirm the prognostic value of MGMT promoter methylation in glioblastoma patients initially treated with TMZ. SQ-MSP allowed better discrimination than classical MSP, and pyrosequencing represented a good option. MGMT methylation (dpeaa)DE-He213 Temozolomide (dpeaa)DE-He213 Glioblastoma (dpeaa)DE-He213 Overall survival (dpeaa)DE-He213 Quillien, Véronique verfasserin aut Guilhot, Joëlle verfasserin aut Wager, Michel verfasserin aut Fromont, Gaëlle verfasserin aut Saikali, Stephan verfasserin aut Etcheverry, Amandine verfasserin aut Hamlat, Abderrahmane verfasserin aut Loussouarn, Delphine verfasserin aut Campion, Loïc verfasserin aut Campone, Mario verfasserin aut Vallette, François-Marie verfasserin aut Gratas-Rabbia-Ré, Catherine verfasserin aut Enthalten in Journal of neuro-oncology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1983 97(2009), 3 vom: 20. Okt., Seite 311-322 (DE-627)32046122X (DE-600)2007293-4 1573-7373 nnns volume:97 year:2009 number:3 day:20 month:10 pages:311-322 https://dx.doi.org/10.1007/s11060-009-0031-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.81 ASE 44.90 ASE AR 97 2009 3 20 10 311-322 |
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10.1007/s11060-009-0031-1 doi (DE-627)SPR016165551 (SPR)s11060-009-0031-1-e DE-627 ger DE-627 rakwb eng 610 ASE 44.81 bkl 44.90 bkl Karayan-Tapon, Lucie verfasserin aut Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This multicenter study assesses the value of $ O^{6} $-methylguanine-DNA methyltransferase (MGMT) status for predicting overall survival in glioblastoma patients. Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients with glioblastomas treated by surgery and radiotherapy with concomitant temozolomide (TMZ) followed by adjuvant TMZ. MGMT promoter methylation was assessed by qualitative methyl-specific polymerase chain reaction (MSP), semiquantitative methyl-specific polymerase chain reaction (SQ-MSP), and pyrosequencing, while MGMT expression was measured at the RNA level by quantitative real-time PCR (Q-RT-PCR) and at the protein level by immunohistochemistry (IHC). MGMT promoter methylation as evaluated by MSP, SQ-MSP, and pyrosequencing was significantly correlated with overall survival. The best predictive value was obtained by pyrosequencing of one specific CpG position. Overall survival was 14 and 25 months for patients with percentages of methylation below and above the median, respectively. In contrast, MGMT status determined by Q-RT-PCR and IHC showed little or no correlation with overall survival, respectively. These results confirm the prognostic value of MGMT promoter methylation in glioblastoma patients initially treated with TMZ. SQ-MSP allowed better discrimination than classical MSP, and pyrosequencing represented a good option. MGMT methylation (dpeaa)DE-He213 Temozolomide (dpeaa)DE-He213 Glioblastoma (dpeaa)DE-He213 Overall survival (dpeaa)DE-He213 Quillien, Véronique verfasserin aut Guilhot, Joëlle verfasserin aut Wager, Michel verfasserin aut Fromont, Gaëlle verfasserin aut Saikali, Stephan verfasserin aut Etcheverry, Amandine verfasserin aut Hamlat, Abderrahmane verfasserin aut Loussouarn, Delphine verfasserin aut Campion, Loïc verfasserin aut Campone, Mario verfasserin aut Vallette, François-Marie verfasserin aut Gratas-Rabbia-Ré, Catherine verfasserin aut Enthalten in Journal of neuro-oncology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1983 97(2009), 3 vom: 20. Okt., Seite 311-322 (DE-627)32046122X (DE-600)2007293-4 1573-7373 nnns volume:97 year:2009 number:3 day:20 month:10 pages:311-322 https://dx.doi.org/10.1007/s11060-009-0031-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.81 ASE 44.90 ASE AR 97 2009 3 20 10 311-322 |
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10.1007/s11060-009-0031-1 doi (DE-627)SPR016165551 (SPR)s11060-009-0031-1-e DE-627 ger DE-627 rakwb eng 610 ASE 44.81 bkl 44.90 bkl Karayan-Tapon, Lucie verfasserin aut Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This multicenter study assesses the value of $ O^{6} $-methylguanine-DNA methyltransferase (MGMT) status for predicting overall survival in glioblastoma patients. Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients with glioblastomas treated by surgery and radiotherapy with concomitant temozolomide (TMZ) followed by adjuvant TMZ. MGMT promoter methylation was assessed by qualitative methyl-specific polymerase chain reaction (MSP), semiquantitative methyl-specific polymerase chain reaction (SQ-MSP), and pyrosequencing, while MGMT expression was measured at the RNA level by quantitative real-time PCR (Q-RT-PCR) and at the protein level by immunohistochemistry (IHC). MGMT promoter methylation as evaluated by MSP, SQ-MSP, and pyrosequencing was significantly correlated with overall survival. The best predictive value was obtained by pyrosequencing of one specific CpG position. Overall survival was 14 and 25 months for patients with percentages of methylation below and above the median, respectively. In contrast, MGMT status determined by Q-RT-PCR and IHC showed little or no correlation with overall survival, respectively. These results confirm the prognostic value of MGMT promoter methylation in glioblastoma patients initially treated with TMZ. SQ-MSP allowed better discrimination than classical MSP, and pyrosequencing represented a good option. MGMT methylation (dpeaa)DE-He213 Temozolomide (dpeaa)DE-He213 Glioblastoma (dpeaa)DE-He213 Overall survival (dpeaa)DE-He213 Quillien, Véronique verfasserin aut Guilhot, Joëlle verfasserin aut Wager, Michel verfasserin aut Fromont, Gaëlle verfasserin aut Saikali, Stephan verfasserin aut Etcheverry, Amandine verfasserin aut Hamlat, Abderrahmane verfasserin aut Loussouarn, Delphine verfasserin aut Campion, Loïc verfasserin aut Campone, Mario verfasserin aut Vallette, François-Marie verfasserin aut Gratas-Rabbia-Ré, Catherine verfasserin aut Enthalten in Journal of neuro-oncology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1983 97(2009), 3 vom: 20. Okt., Seite 311-322 (DE-627)32046122X (DE-600)2007293-4 1573-7373 nnns volume:97 year:2009 number:3 day:20 month:10 pages:311-322 https://dx.doi.org/10.1007/s11060-009-0031-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.81 ASE 44.90 ASE AR 97 2009 3 20 10 311-322 |
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Enthalten in Journal of neuro-oncology 97(2009), 3 vom: 20. Okt., Seite 311-322 volume:97 year:2009 number:3 day:20 month:10 pages:311-322 |
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Enthalten in Journal of neuro-oncology 97(2009), 3 vom: 20. Okt., Seite 311-322 volume:97 year:2009 number:3 day:20 month:10 pages:311-322 |
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findex.gbv.de |
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MGMT methylation Temozolomide Glioblastoma Overall survival |
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Journal of neuro-oncology |
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Karayan-Tapon, Lucie @@aut@@ Quillien, Véronique @@aut@@ Guilhot, Joëlle @@aut@@ Wager, Michel @@aut@@ Fromont, Gaëlle @@aut@@ Saikali, Stephan @@aut@@ Etcheverry, Amandine @@aut@@ Hamlat, Abderrahmane @@aut@@ Loussouarn, Delphine @@aut@@ Campion, Loïc @@aut@@ Campone, Mario @@aut@@ Vallette, François-Marie @@aut@@ Gratas-Rabbia-Ré, Catherine @@aut@@ |
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2009-10-20T00:00:00Z |
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Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients with glioblastomas treated by surgery and radiotherapy with concomitant temozolomide (TMZ) followed by adjuvant TMZ. MGMT promoter methylation was assessed by qualitative methyl-specific polymerase chain reaction (MSP), semiquantitative methyl-specific polymerase chain reaction (SQ-MSP), and pyrosequencing, while MGMT expression was measured at the RNA level by quantitative real-time PCR (Q-RT-PCR) and at the protein level by immunohistochemistry (IHC). MGMT promoter methylation as evaluated by MSP, SQ-MSP, and pyrosequencing was significantly correlated with overall survival. The best predictive value was obtained by pyrosequencing of one specific CpG position. Overall survival was 14 and 25 months for patients with percentages of methylation below and above the median, respectively. In contrast, MGMT status determined by Q-RT-PCR and IHC showed little or no correlation with overall survival, respectively. These results confirm the prognostic value of MGMT promoter methylation in glioblastoma patients initially treated with TMZ. 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|
| author |
Karayan-Tapon, Lucie |
| spellingShingle |
Karayan-Tapon, Lucie ddc 610 bkl 44.81 bkl 44.90 misc MGMT methylation misc Temozolomide misc Glioblastoma misc Overall survival Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods |
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Karayan-Tapon, Lucie |
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electronic Article |
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610 - Medicine & health |
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keep |
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springer |
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Not Illustrated |
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1573-7373 |
| topic_title |
610 ASE 44.81 bkl 44.90 bkl Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods MGMT methylation (dpeaa)DE-He213 Temozolomide (dpeaa)DE-He213 Glioblastoma (dpeaa)DE-He213 Overall survival (dpeaa)DE-He213 |
| topic |
ddc 610 bkl 44.81 bkl 44.90 misc MGMT methylation misc Temozolomide misc Glioblastoma misc Overall survival |
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ddc 610 bkl 44.81 bkl 44.90 misc MGMT methylation misc Temozolomide misc Glioblastoma misc Overall survival |
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ddc 610 bkl 44.81 bkl 44.90 misc MGMT methylation misc Temozolomide misc Glioblastoma misc Overall survival |
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Elektronische Aufsätze Aufsätze Elektronische Ressource |
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Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods |
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Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods |
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Karayan-Tapon, Lucie Quillien, Véronique Guilhot, Joëlle Wager, Michel Fromont, Gaëlle Saikali, Stephan Etcheverry, Amandine Hamlat, Abderrahmane Loussouarn, Delphine Campion, Loïc Campone, Mario Vallette, François-Marie Gratas-Rabbia-Ré, Catherine |
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prognostic value of $ o^{6} $-methylguanine-dna methyltransferase status in glioblastoma patients, assessed by five different methods |
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Prognostic value of $ O^{6} $-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods |
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Abstract This multicenter study assesses the value of $ O^{6} $-methylguanine-DNA methyltransferase (MGMT) status for predicting overall survival in glioblastoma patients. Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients with glioblastomas treated by surgery and radiotherapy with concomitant temozolomide (TMZ) followed by adjuvant TMZ. MGMT promoter methylation was assessed by qualitative methyl-specific polymerase chain reaction (MSP), semiquantitative methyl-specific polymerase chain reaction (SQ-MSP), and pyrosequencing, while MGMT expression was measured at the RNA level by quantitative real-time PCR (Q-RT-PCR) and at the protein level by immunohistochemistry (IHC). MGMT promoter methylation as evaluated by MSP, SQ-MSP, and pyrosequencing was significantly correlated with overall survival. The best predictive value was obtained by pyrosequencing of one specific CpG position. Overall survival was 14 and 25 months for patients with percentages of methylation below and above the median, respectively. In contrast, MGMT status determined by Q-RT-PCR and IHC showed little or no correlation with overall survival, respectively. These results confirm the prognostic value of MGMT promoter methylation in glioblastoma patients initially treated with TMZ. SQ-MSP allowed better discrimination than classical MSP, and pyrosequencing represented a good option. |
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Abstract This multicenter study assesses the value of $ O^{6} $-methylguanine-DNA methyltransferase (MGMT) status for predicting overall survival in glioblastoma patients. Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients with glioblastomas treated by surgery and radiotherapy with concomitant temozolomide (TMZ) followed by adjuvant TMZ. MGMT promoter methylation was assessed by qualitative methyl-specific polymerase chain reaction (MSP), semiquantitative methyl-specific polymerase chain reaction (SQ-MSP), and pyrosequencing, while MGMT expression was measured at the RNA level by quantitative real-time PCR (Q-RT-PCR) and at the protein level by immunohistochemistry (IHC). MGMT promoter methylation as evaluated by MSP, SQ-MSP, and pyrosequencing was significantly correlated with overall survival. The best predictive value was obtained by pyrosequencing of one specific CpG position. Overall survival was 14 and 25 months for patients with percentages of methylation below and above the median, respectively. In contrast, MGMT status determined by Q-RT-PCR and IHC showed little or no correlation with overall survival, respectively. These results confirm the prognostic value of MGMT promoter methylation in glioblastoma patients initially treated with TMZ. SQ-MSP allowed better discrimination than classical MSP, and pyrosequencing represented a good option. |
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Abstract This multicenter study assesses the value of $ O^{6} $-methylguanine-DNA methyltransferase (MGMT) status for predicting overall survival in glioblastoma patients. Five methods are used, to identify the approach with the best prognostic value. Eighty-one tumors were obtained from patients with glioblastomas treated by surgery and radiotherapy with concomitant temozolomide (TMZ) followed by adjuvant TMZ. MGMT promoter methylation was assessed by qualitative methyl-specific polymerase chain reaction (MSP), semiquantitative methyl-specific polymerase chain reaction (SQ-MSP), and pyrosequencing, while MGMT expression was measured at the RNA level by quantitative real-time PCR (Q-RT-PCR) and at the protein level by immunohistochemistry (IHC). MGMT promoter methylation as evaluated by MSP, SQ-MSP, and pyrosequencing was significantly correlated with overall survival. The best predictive value was obtained by pyrosequencing of one specific CpG position. Overall survival was 14 and 25 months for patients with percentages of methylation below and above the median, respectively. In contrast, MGMT status determined by Q-RT-PCR and IHC showed little or no correlation with overall survival, respectively. These results confirm the prognostic value of MGMT promoter methylation in glioblastoma patients initially treated with TMZ. SQ-MSP allowed better discrimination than classical MSP, and pyrosequencing represented a good option. |
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| score |
7.401457 |