Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia

Abstract The current study was designed to initially observe the changes in soluble proteome in rat hippocampus post anesthesia, trying to explore possible clues for elucidating the effects of propofol. Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were o...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Zhang, Xuena [verfasserIn] Yang, Shuguang [verfasserIn] Yue, Yun [verfasserIn] Wu, Anshi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	Hippocampus Propofol Soluble proteome

Übergeordnetes Werk:	Enthalten in: Neurochemical research - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1976, 38(2013), 12 vom: 09. Nov., Seite 2661-2667
Übergeordnetes Werk:	volume:38 ; year:2013 ; number:12 ; day:09 ; month:11 ; pages:2661-2667

Links:	Volltext

DOI / URN:	10.1007/s11064-013-1184-z

Katalog-ID:	SPR016257197

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520			\|a Abstract The current study was designed to initially observe the changes in soluble proteome in rat hippocampus post anesthesia, trying to explore possible clues for elucidating the effects of propofol. Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were observed at 1, 6, 24 h and 7 days after 3 h of propofol anesthesia. Spots exhibiting significant changes among different time-points were submitted to matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS) assay and peptide mass fingerprinting identification. The expression changes of selected proteins were further assayed using Western blot and RT-PCR. Twenty-six differentially expressed proteins were found and 19 were successfully identified with MALDI-TOF MS. Gene ontology analysis revealed these identified proteins were mainly cytosol (5) and/or cytoskeleton fractions (5). According to biological processes category, 9 proteins take part in development process, 12 are involved in metabolic process and 6 in regulatory function. Functionally, 17 proteins were involved in binding activities among which 12 possessed catalytic activities. Most changes took place within 24 h. Change patterns of selected proteins were identical in 2-DE and Western blot. Three mRNA of 5 selected proteins exhibited similar change patterns with those of their protein expressions. Soluble proteome in rat hippocampus are dynamically affected by propofol, with multiple processes being involved. They are possible explanations for propofol effects but further investigations are required.
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allfields	10.1007/s11064-013-1184-z doi (DE-627)SPR016257197 (SPR)s11064-013-1184-z-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Zhang, Xuena verfasserin aut Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The current study was designed to initially observe the changes in soluble proteome in rat hippocampus post anesthesia, trying to explore possible clues for elucidating the effects of propofol. Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were observed at 1, 6, 24 h and 7 days after 3 h of propofol anesthesia. Spots exhibiting significant changes among different time-points were submitted to matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS) assay and peptide mass fingerprinting identification. The expression changes of selected proteins were further assayed using Western blot and RT-PCR. Twenty-six differentially expressed proteins were found and 19 were successfully identified with MALDI-TOF MS. Gene ontology analysis revealed these identified proteins were mainly cytosol (5) and/or cytoskeleton fractions (5). According to biological processes category, 9 proteins take part in development process, 12 are involved in metabolic process and 6 in regulatory function. Functionally, 17 proteins were involved in binding activities among which 12 possessed catalytic activities. Most changes took place within 24 h. Change patterns of selected proteins were identical in 2-DE and Western blot. Three mRNA of 5 selected proteins exhibited similar change patterns with those of their protein expressions. Soluble proteome in rat hippocampus are dynamically affected by propofol, with multiple processes being involved. They are possible explanations for propofol effects but further investigations are required. Hippocampus (dpeaa)DE-He213 Propofol (dpeaa)DE-He213 Soluble proteome (dpeaa)DE-He213 Yang, Shuguang verfasserin aut Yue, Yun verfasserin aut Wu, Anshi verfasserin aut Enthalten in Neurochemical research Dordrecht [u.a.] : Springer Science + Business Media B.V, 1976 38(2013), 12 vom: 09. Nov., Seite 2661-2667 (DE-627)320587770 (DE-600)2018503-0 1573-6903 nnns volume:38 year:2013 number:12 day:09 month:11 pages:2661-2667 https://dx.doi.org/10.1007/s11064-013-1184-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 38 2013 12 09 11 2661-2667
spelling	10.1007/s11064-013-1184-z doi (DE-627)SPR016257197 (SPR)s11064-013-1184-z-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Zhang, Xuena verfasserin aut Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The current study was designed to initially observe the changes in soluble proteome in rat hippocampus post anesthesia, trying to explore possible clues for elucidating the effects of propofol. Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were observed at 1, 6, 24 h and 7 days after 3 h of propofol anesthesia. Spots exhibiting significant changes among different time-points were submitted to matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS) assay and peptide mass fingerprinting identification. The expression changes of selected proteins were further assayed using Western blot and RT-PCR. Twenty-six differentially expressed proteins were found and 19 were successfully identified with MALDI-TOF MS. Gene ontology analysis revealed these identified proteins were mainly cytosol (5) and/or cytoskeleton fractions (5). According to biological processes category, 9 proteins take part in development process, 12 are involved in metabolic process and 6 in regulatory function. Functionally, 17 proteins were involved in binding activities among which 12 possessed catalytic activities. Most changes took place within 24 h. Change patterns of selected proteins were identical in 2-DE and Western blot. Three mRNA of 5 selected proteins exhibited similar change patterns with those of their protein expressions. Soluble proteome in rat hippocampus are dynamically affected by propofol, with multiple processes being involved. They are possible explanations for propofol effects but further investigations are required. Hippocampus (dpeaa)DE-He213 Propofol (dpeaa)DE-He213 Soluble proteome (dpeaa)DE-He213 Yang, Shuguang verfasserin aut Yue, Yun verfasserin aut Wu, Anshi verfasserin aut Enthalten in Neurochemical research Dordrecht [u.a.] : Springer Science + Business Media B.V, 1976 38(2013), 12 vom: 09. Nov., Seite 2661-2667 (DE-627)320587770 (DE-600)2018503-0 1573-6903 nnns volume:38 year:2013 number:12 day:09 month:11 pages:2661-2667 https://dx.doi.org/10.1007/s11064-013-1184-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 38 2013 12 09 11 2661-2667
allfields_unstemmed	10.1007/s11064-013-1184-z doi (DE-627)SPR016257197 (SPR)s11064-013-1184-z-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Zhang, Xuena verfasserin aut Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The current study was designed to initially observe the changes in soluble proteome in rat hippocampus post anesthesia, trying to explore possible clues for elucidating the effects of propofol. Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were observed at 1, 6, 24 h and 7 days after 3 h of propofol anesthesia. Spots exhibiting significant changes among different time-points were submitted to matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS) assay and peptide mass fingerprinting identification. The expression changes of selected proteins were further assayed using Western blot and RT-PCR. Twenty-six differentially expressed proteins were found and 19 were successfully identified with MALDI-TOF MS. Gene ontology analysis revealed these identified proteins were mainly cytosol (5) and/or cytoskeleton fractions (5). According to biological processes category, 9 proteins take part in development process, 12 are involved in metabolic process and 6 in regulatory function. Functionally, 17 proteins were involved in binding activities among which 12 possessed catalytic activities. Most changes took place within 24 h. Change patterns of selected proteins were identical in 2-DE and Western blot. Three mRNA of 5 selected proteins exhibited similar change patterns with those of their protein expressions. Soluble proteome in rat hippocampus are dynamically affected by propofol, with multiple processes being involved. They are possible explanations for propofol effects but further investigations are required. Hippocampus (dpeaa)DE-He213 Propofol (dpeaa)DE-He213 Soluble proteome (dpeaa)DE-He213 Yang, Shuguang verfasserin aut Yue, Yun verfasserin aut Wu, Anshi verfasserin aut Enthalten in Neurochemical research Dordrecht [u.a.] : Springer Science + Business Media B.V, 1976 38(2013), 12 vom: 09. Nov., Seite 2661-2667 (DE-627)320587770 (DE-600)2018503-0 1573-6903 nnns volume:38 year:2013 number:12 day:09 month:11 pages:2661-2667 https://dx.doi.org/10.1007/s11064-013-1184-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 38 2013 12 09 11 2661-2667
allfieldsGer	10.1007/s11064-013-1184-z doi (DE-627)SPR016257197 (SPR)s11064-013-1184-z-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Zhang, Xuena verfasserin aut Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The current study was designed to initially observe the changes in soluble proteome in rat hippocampus post anesthesia, trying to explore possible clues for elucidating the effects of propofol. Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were observed at 1, 6, 24 h and 7 days after 3 h of propofol anesthesia. Spots exhibiting significant changes among different time-points were submitted to matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS) assay and peptide mass fingerprinting identification. The expression changes of selected proteins were further assayed using Western blot and RT-PCR. Twenty-six differentially expressed proteins were found and 19 were successfully identified with MALDI-TOF MS. Gene ontology analysis revealed these identified proteins were mainly cytosol (5) and/or cytoskeleton fractions (5). According to biological processes category, 9 proteins take part in development process, 12 are involved in metabolic process and 6 in regulatory function. Functionally, 17 proteins were involved in binding activities among which 12 possessed catalytic activities. Most changes took place within 24 h. Change patterns of selected proteins were identical in 2-DE and Western blot. Three mRNA of 5 selected proteins exhibited similar change patterns with those of their protein expressions. Soluble proteome in rat hippocampus are dynamically affected by propofol, with multiple processes being involved. They are possible explanations for propofol effects but further investigations are required. Hippocampus (dpeaa)DE-He213 Propofol (dpeaa)DE-He213 Soluble proteome (dpeaa)DE-He213 Yang, Shuguang verfasserin aut Yue, Yun verfasserin aut Wu, Anshi verfasserin aut Enthalten in Neurochemical research Dordrecht [u.a.] : Springer Science + Business Media B.V, 1976 38(2013), 12 vom: 09. Nov., Seite 2661-2667 (DE-627)320587770 (DE-600)2018503-0 1573-6903 nnns volume:38 year:2013 number:12 day:09 month:11 pages:2661-2667 https://dx.doi.org/10.1007/s11064-013-1184-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 38 2013 12 09 11 2661-2667
allfieldsSound	10.1007/s11064-013-1184-z doi (DE-627)SPR016257197 (SPR)s11064-013-1184-z-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Zhang, Xuena verfasserin aut Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The current study was designed to initially observe the changes in soluble proteome in rat hippocampus post anesthesia, trying to explore possible clues for elucidating the effects of propofol. Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were observed at 1, 6, 24 h and 7 days after 3 h of propofol anesthesia. Spots exhibiting significant changes among different time-points were submitted to matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS) assay and peptide mass fingerprinting identification. The expression changes of selected proteins were further assayed using Western blot and RT-PCR. Twenty-six differentially expressed proteins were found and 19 were successfully identified with MALDI-TOF MS. Gene ontology analysis revealed these identified proteins were mainly cytosol (5) and/or cytoskeleton fractions (5). According to biological processes category, 9 proteins take part in development process, 12 are involved in metabolic process and 6 in regulatory function. Functionally, 17 proteins were involved in binding activities among which 12 possessed catalytic activities. Most changes took place within 24 h. Change patterns of selected proteins were identical in 2-DE and Western blot. Three mRNA of 5 selected proteins exhibited similar change patterns with those of their protein expressions. Soluble proteome in rat hippocampus are dynamically affected by propofol, with multiple processes being involved. They are possible explanations for propofol effects but further investigations are required. Hippocampus (dpeaa)DE-He213 Propofol (dpeaa)DE-He213 Soluble proteome (dpeaa)DE-He213 Yang, Shuguang verfasserin aut Yue, Yun verfasserin aut Wu, Anshi verfasserin aut Enthalten in Neurochemical research Dordrecht [u.a.] : Springer Science + Business Media B.V, 1976 38(2013), 12 vom: 09. Nov., Seite 2661-2667 (DE-627)320587770 (DE-600)2018503-0 1573-6903 nnns volume:38 year:2013 number:12 day:09 month:11 pages:2661-2667 https://dx.doi.org/10.1007/s11064-013-1184-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 38 2013 12 09 11 2661-2667
language	English
source	Enthalten in Neurochemical research 38(2013), 12 vom: 09. Nov., Seite 2661-2667 volume:38 year:2013 number:12 day:09 month:11 pages:2661-2667
sourceStr	Enthalten in Neurochemical research 38(2013), 12 vom: 09. Nov., Seite 2661-2667 volume:38 year:2013 number:12 day:09 month:11 pages:2661-2667
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Hippocampus Propofol Soluble proteome
dewey-raw	610
isfreeaccess_bool	false
container_title	Neurochemical research
authorswithroles_txt_mv	Zhang, Xuena @@aut@@ Yang, Shuguang @@aut@@ Yue, Yun @@aut@@ Wu, Anshi @@aut@@
publishDateDaySort_date	2013-11-09T00:00:00Z
hierarchy_top_id	320587770
dewey-sort	3610
id	SPR016257197
language_de	englisch
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author	Zhang, Xuena
spellingShingle	Zhang, Xuena ddc 610 bkl 44.90 misc Hippocampus misc Propofol misc Soluble proteome Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia
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topic_title	610 ASE 44.90 bkl Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia Hippocampus (dpeaa)DE-He213 Propofol (dpeaa)DE-He213 Soluble proteome (dpeaa)DE-He213
topic	ddc 610 bkl 44.90 misc Hippocampus misc Propofol misc Soluble proteome
topic_unstemmed	ddc 610 bkl 44.90 misc Hippocampus misc Propofol misc Soluble proteome
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title	Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia
ctrlnum	(DE-627)SPR016257197 (SPR)s11064-013-1184-z-e
title_full	Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia
author_sort	Zhang, Xuena
journal	Neurochemical research
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author_browse	Zhang, Xuena Yang, Shuguang Yue, Yun Wu, Anshi
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title_sort	profiling of the soluble proteome in rat hippocampus post propofol anesthesia
title_auth	Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia
abstract	Abstract The current study was designed to initially observe the changes in soluble proteome in rat hippocampus post anesthesia, trying to explore possible clues for elucidating the effects of propofol. Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were observed at 1, 6, 24 h and 7 days after 3 h of propofol anesthesia. Spots exhibiting significant changes among different time-points were submitted to matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS) assay and peptide mass fingerprinting identification. The expression changes of selected proteins were further assayed using Western blot and RT-PCR. Twenty-six differentially expressed proteins were found and 19 were successfully identified with MALDI-TOF MS. Gene ontology analysis revealed these identified proteins were mainly cytosol (5) and/or cytoskeleton fractions (5). According to biological processes category, 9 proteins take part in development process, 12 are involved in metabolic process and 6 in regulatory function. Functionally, 17 proteins were involved in binding activities among which 12 possessed catalytic activities. Most changes took place within 24 h. Change patterns of selected proteins were identical in 2-DE and Western blot. Three mRNA of 5 selected proteins exhibited similar change patterns with those of their protein expressions. Soluble proteome in rat hippocampus are dynamically affected by propofol, with multiple processes being involved. They are possible explanations for propofol effects but further investigations are required.
abstractGer	Abstract The current study was designed to initially observe the changes in soluble proteome in rat hippocampus post anesthesia, trying to explore possible clues for elucidating the effects of propofol. Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were observed at 1, 6, 24 h and 7 days after 3 h of propofol anesthesia. Spots exhibiting significant changes among different time-points were submitted to matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS) assay and peptide mass fingerprinting identification. The expression changes of selected proteins were further assayed using Western blot and RT-PCR. Twenty-six differentially expressed proteins were found and 19 were successfully identified with MALDI-TOF MS. Gene ontology analysis revealed these identified proteins were mainly cytosol (5) and/or cytoskeleton fractions (5). According to biological processes category, 9 proteins take part in development process, 12 are involved in metabolic process and 6 in regulatory function. Functionally, 17 proteins were involved in binding activities among which 12 possessed catalytic activities. Most changes took place within 24 h. Change patterns of selected proteins were identical in 2-DE and Western blot. Three mRNA of 5 selected proteins exhibited similar change patterns with those of their protein expressions. Soluble proteome in rat hippocampus are dynamically affected by propofol, with multiple processes being involved. They are possible explanations for propofol effects but further investigations are required.
abstract_unstemmed	Abstract The current study was designed to initially observe the changes in soluble proteome in rat hippocampus post anesthesia, trying to explore possible clues for elucidating the effects of propofol. Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were observed at 1, 6, 24 h and 7 days after 3 h of propofol anesthesia. Spots exhibiting significant changes among different time-points were submitted to matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS) assay and peptide mass fingerprinting identification. The expression changes of selected proteins were further assayed using Western blot and RT-PCR. Twenty-six differentially expressed proteins were found and 19 were successfully identified with MALDI-TOF MS. Gene ontology analysis revealed these identified proteins were mainly cytosol (5) and/or cytoskeleton fractions (5). According to biological processes category, 9 proteins take part in development process, 12 are involved in metabolic process and 6 in regulatory function. Functionally, 17 proteins were involved in binding activities among which 12 possessed catalytic activities. Most changes took place within 24 h. Change patterns of selected proteins were identical in 2-DE and Western blot. Three mRNA of 5 selected proteins exhibited similar change patterns with those of their protein expressions. Soluble proteome in rat hippocampus are dynamically affected by propofol, with multiple processes being involved. They are possible explanations for propofol effects but further investigations are required.
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title_short	Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia
url	https://dx.doi.org/10.1007/s11064-013-1184-z
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author2	Yang, Shuguang Yue, Yun Wu, Anshi
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up_date	2024-07-03T21:55:34.081Z
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Soluble proteins were separated by 2-dimensional electrophoresis (2-DE). Their expressions were observed at 1, 6, 24 h and 7 days after 3 h of propofol anesthesia. Spots exhibiting significant changes among different time-points were submitted to matrix-assisted laser desorption/ionization time of flight mass spectrometer (MALDI-TOF MS) assay and peptide mass fingerprinting identification. The expression changes of selected proteins were further assayed using Western blot and RT-PCR. Twenty-six differentially expressed proteins were found and 19 were successfully identified with MALDI-TOF MS. Gene ontology analysis revealed these identified proteins were mainly cytosol (5) and/or cytoskeleton fractions (5). According to biological processes category, 9 proteins take part in development process, 12 are involved in metabolic process and 6 in regulatory function. Functionally, 17 proteins were involved in binding activities among which 12 possessed catalytic activities. Most changes took place within 24 h. Change patterns of selected proteins were identical in 2-DE and Western blot. Three mRNA of 5 selected proteins exhibited similar change patterns with those of their protein expressions. Soluble proteome in rat hippocampus are dynamically affected by propofol, with multiple processes being involved. 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Profiling of the Soluble Proteome in Rat Hippocampus Post Propofol Anesthesia

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