Cloning and expression of ophc2, a new organphosphorus hydrolase gene

Abstract The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences an...
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Gespeichert in:

Autor*in:	Ningfeng, Wu [verfasserIn] Minjie, Deng [verfasserIn] Guoyi, Liang [verfasserIn] Xiaoyu, Chu [verfasserIn] Bin, Yao [verfasserIn] Yunliu, Fan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2004

Schlagwörter:	organphosphorus hydrolase gene cloning and expression

Übergeordnetes Werk:	Enthalten in: Chinese science bulletin - Beijing, China : Chinese Acad. of Sciences, 1997, 49(2004), 12 vom: Juni, Seite 1245-1249
Übergeordnetes Werk:	volume:49 ; year:2004 ; number:12 ; month:06 ; pages:1245-1249

Links:	Volltext

DOI / URN:	10.1360/04wc0146

Katalog-ID:	SPR019372604

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520			\|a Abstract The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence of ophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity.
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allfields	10.1360/04wc0146 doi (DE-627)SPR019372604 (SPR)04wc0146-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Ningfeng, Wu verfasserin aut Cloning and expression of ophc2, a new organphosphorus hydrolase gene 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence of ophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity. organphosphorus hydrolase (dpeaa)DE-He213 gene cloning and expression (dpeaa)DE-He213 Minjie, Deng verfasserin aut Guoyi, Liang verfasserin aut Xiaoyu, Chu verfasserin aut Bin, Yao verfasserin aut Yunliu, Fan verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 49(2004), 12 vom: Juni, Seite 1245-1249 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:49 year:2004 number:12 month:06 pages:1245-1249 https://dx.doi.org/10.1360/04wc0146 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_40 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_110 GBV_ILN_120 GBV_ILN_161 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 49 2004 12 06 1245-1249
spelling	10.1360/04wc0146 doi (DE-627)SPR019372604 (SPR)04wc0146-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Ningfeng, Wu verfasserin aut Cloning and expression of ophc2, a new organphosphorus hydrolase gene 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence of ophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity. organphosphorus hydrolase (dpeaa)DE-He213 gene cloning and expression (dpeaa)DE-He213 Minjie, Deng verfasserin aut Guoyi, Liang verfasserin aut Xiaoyu, Chu verfasserin aut Bin, Yao verfasserin aut Yunliu, Fan verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 49(2004), 12 vom: Juni, Seite 1245-1249 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:49 year:2004 number:12 month:06 pages:1245-1249 https://dx.doi.org/10.1360/04wc0146 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_40 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_110 GBV_ILN_120 GBV_ILN_161 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 49 2004 12 06 1245-1249
allfields_unstemmed	10.1360/04wc0146 doi (DE-627)SPR019372604 (SPR)04wc0146-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Ningfeng, Wu verfasserin aut Cloning and expression of ophc2, a new organphosphorus hydrolase gene 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence of ophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity. organphosphorus hydrolase (dpeaa)DE-He213 gene cloning and expression (dpeaa)DE-He213 Minjie, Deng verfasserin aut Guoyi, Liang verfasserin aut Xiaoyu, Chu verfasserin aut Bin, Yao verfasserin aut Yunliu, Fan verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 49(2004), 12 vom: Juni, Seite 1245-1249 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:49 year:2004 number:12 month:06 pages:1245-1249 https://dx.doi.org/10.1360/04wc0146 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_40 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_110 GBV_ILN_120 GBV_ILN_161 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 49 2004 12 06 1245-1249
allfieldsGer	10.1360/04wc0146 doi (DE-627)SPR019372604 (SPR)04wc0146-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Ningfeng, Wu verfasserin aut Cloning and expression of ophc2, a new organphosphorus hydrolase gene 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence of ophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity. organphosphorus hydrolase (dpeaa)DE-He213 gene cloning and expression (dpeaa)DE-He213 Minjie, Deng verfasserin aut Guoyi, Liang verfasserin aut Xiaoyu, Chu verfasserin aut Bin, Yao verfasserin aut Yunliu, Fan verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 49(2004), 12 vom: Juni, Seite 1245-1249 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:49 year:2004 number:12 month:06 pages:1245-1249 https://dx.doi.org/10.1360/04wc0146 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_40 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_110 GBV_ILN_120 GBV_ILN_161 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 49 2004 12 06 1245-1249
allfieldsSound	10.1360/04wc0146 doi (DE-627)SPR019372604 (SPR)04wc0146-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Ningfeng, Wu verfasserin aut Cloning and expression of ophc2, a new organphosphorus hydrolase gene 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence of ophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity. organphosphorus hydrolase (dpeaa)DE-He213 gene cloning and expression (dpeaa)DE-He213 Minjie, Deng verfasserin aut Guoyi, Liang verfasserin aut Xiaoyu, Chu verfasserin aut Bin, Yao verfasserin aut Yunliu, Fan verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 49(2004), 12 vom: Juni, Seite 1245-1249 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:49 year:2004 number:12 month:06 pages:1245-1249 https://dx.doi.org/10.1360/04wc0146 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_40 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_110 GBV_ILN_120 GBV_ILN_161 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 49 2004 12 06 1245-1249
language	English
source	Enthalten in Chinese science bulletin 49(2004), 12 vom: Juni, Seite 1245-1249 volume:49 year:2004 number:12 month:06 pages:1245-1249
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authorswithroles_txt_mv	Ningfeng, Wu @@aut@@ Minjie, Deng @@aut@@ Guoyi, Liang @@aut@@ Xiaoyu, Chu @@aut@@ Bin, Yao @@aut@@ Yunliu, Fan @@aut@@
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author	Ningfeng, Wu
spellingShingle	Ningfeng, Wu ddc 500 bkl 30.00 misc organphosphorus hydrolase misc gene cloning and expression Cloning and expression of ophc2, a new organphosphorus hydrolase gene
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topic_title	500 ASE 30.00 bkl Cloning and expression of ophc2, a new organphosphorus hydrolase gene organphosphorus hydrolase (dpeaa)DE-He213 gene cloning and expression (dpeaa)DE-He213
topic	ddc 500 bkl 30.00 misc organphosphorus hydrolase misc gene cloning and expression
topic_unstemmed	ddc 500 bkl 30.00 misc organphosphorus hydrolase misc gene cloning and expression
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title	Cloning and expression of ophc2, a new organphosphorus hydrolase gene
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title_full	Cloning and expression of ophc2, a new organphosphorus hydrolase gene
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author_browse	Ningfeng, Wu Minjie, Deng Guoyi, Liang Xiaoyu, Chu Bin, Yao Yunliu, Fan
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title_sort	cloning and expression of ophc2, a new organphosphorus hydrolase gene
title_auth	Cloning and expression of ophc2, a new organphosphorus hydrolase gene
abstract	Abstract The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence of ophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity.
abstractGer	Abstract The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence of ophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity.
abstract_unstemmed	Abstract The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide sequence of ophc2 shows low homologies with those organphosphorus hydrolase genes deposited in GenBank, one of which exhibits the highest homology of 46.4% with ophc2. The organphosphorus hydrolase protein expressed in E. coli bears normal bioactivity.
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title_short	Cloning and expression of ophc2, a new organphosphorus hydrolase gene
url	https://dx.doi.org/10.1360/04wc0146
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author2	Minjie, Deng Guoyi, Liang Xiaoyu, Chu Bin, Yao Yunliu, Fan
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up_date	2024-07-04T01:18:04.120Z
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Cloning and expression of ophc2, a new organphosphorus hydrolase gene

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