Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana

Abstract Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drou...
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Gespeichert in:

Autor*in:	Gang, Wei [verfasserIn] Juan, Lei [verfasserIn] Wei, Gong [verfasserIn] Yuxian, Zhu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2005

Schlagwörter:	QRAP2 stress DREB one-hybrid transcription factor

Übergeordnetes Werk:	Enthalten in: Chinese science bulletin - Beijing, China : Chinese Acad. of Sciences, 1997, 50(2005), 17 vom: Sept., Seite 1874-1879
Übergeordnetes Werk:	volume:50 ; year:2005 ; number:17 ; month:09 ; pages:1874-1879

Links:	Volltext

DOI / URN:	10.1360/982005-706

Katalog-ID:	SPR019373945

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520			\|a Abstract Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drought, UV, abscisic acid, high salinity and salicylic acid treatments. It was classified as a DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and contained a glutamine-rich region near its N terminus, we named it QRAP2 (for glutamine-rich AP2). Gel retardation assay revealed that QRAP2 was able to form a specific complex with the previously characterized DRE element while did not show any affinity to the GCC box or the mutant DRE box. When fused to the GAL4 DNA-binding domain, either fulllength QRAP2 or its N-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay with its C-terminus completely inactive. Our data indicate that QRAP2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream target genes in response to environmental stress, especially under drought conditions.
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allfields	10.1360/982005-706 doi (DE-627)SPR019373945 (SPR)982005-706-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Gang, Wei verfasserin aut Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drought, UV, abscisic acid, high salinity and salicylic acid treatments. It was classified as a DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and contained a glutamine-rich region near its N terminus, we named it QRAP2 (for glutamine-rich AP2). Gel retardation assay revealed that QRAP2 was able to form a specific complex with the previously characterized DRE element while did not show any affinity to the GCC box or the mutant DRE box. When fused to the GAL4 DNA-binding domain, either fulllength QRAP2 or its N-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay with its C-terminus completely inactive. Our data indicate that QRAP2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream target genes in response to environmental stress, especially under drought conditions. QRAP2 (dpeaa)DE-He213 stress (dpeaa)DE-He213 DREB (dpeaa)DE-He213 one-hybrid (dpeaa)DE-He213 transcription factor (dpeaa)DE-He213 Juan, Lei verfasserin aut Wei, Gong verfasserin aut Yuxian, Zhu verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 50(2005), 17 vom: Sept., Seite 1874-1879 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:50 year:2005 number:17 month:09 pages:1874-1879 https://dx.doi.org/10.1360/982005-706 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_40 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_110 GBV_ILN_120 GBV_ILN_161 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 50 2005 17 09 1874-1879
spelling	10.1360/982005-706 doi (DE-627)SPR019373945 (SPR)982005-706-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Gang, Wei verfasserin aut Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drought, UV, abscisic acid, high salinity and salicylic acid treatments. It was classified as a DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and contained a glutamine-rich region near its N terminus, we named it QRAP2 (for glutamine-rich AP2). Gel retardation assay revealed that QRAP2 was able to form a specific complex with the previously characterized DRE element while did not show any affinity to the GCC box or the mutant DRE box. When fused to the GAL4 DNA-binding domain, either fulllength QRAP2 or its N-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay with its C-terminus completely inactive. Our data indicate that QRAP2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream target genes in response to environmental stress, especially under drought conditions. QRAP2 (dpeaa)DE-He213 stress (dpeaa)DE-He213 DREB (dpeaa)DE-He213 one-hybrid (dpeaa)DE-He213 transcription factor (dpeaa)DE-He213 Juan, Lei verfasserin aut Wei, Gong verfasserin aut Yuxian, Zhu verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 50(2005), 17 vom: Sept., Seite 1874-1879 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:50 year:2005 number:17 month:09 pages:1874-1879 https://dx.doi.org/10.1360/982005-706 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_40 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_110 GBV_ILN_120 GBV_ILN_161 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 50 2005 17 09 1874-1879
allfields_unstemmed	10.1360/982005-706 doi (DE-627)SPR019373945 (SPR)982005-706-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Gang, Wei verfasserin aut Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drought, UV, abscisic acid, high salinity and salicylic acid treatments. It was classified as a DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and contained a glutamine-rich region near its N terminus, we named it QRAP2 (for glutamine-rich AP2). Gel retardation assay revealed that QRAP2 was able to form a specific complex with the previously characterized DRE element while did not show any affinity to the GCC box or the mutant DRE box. When fused to the GAL4 DNA-binding domain, either fulllength QRAP2 or its N-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay with its C-terminus completely inactive. Our data indicate that QRAP2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream target genes in response to environmental stress, especially under drought conditions. QRAP2 (dpeaa)DE-He213 stress (dpeaa)DE-He213 DREB (dpeaa)DE-He213 one-hybrid (dpeaa)DE-He213 transcription factor (dpeaa)DE-He213 Juan, Lei verfasserin aut Wei, Gong verfasserin aut Yuxian, Zhu verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 50(2005), 17 vom: Sept., Seite 1874-1879 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:50 year:2005 number:17 month:09 pages:1874-1879 https://dx.doi.org/10.1360/982005-706 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_40 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_110 GBV_ILN_120 GBV_ILN_161 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 50 2005 17 09 1874-1879
allfieldsGer	10.1360/982005-706 doi (DE-627)SPR019373945 (SPR)982005-706-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Gang, Wei verfasserin aut Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drought, UV, abscisic acid, high salinity and salicylic acid treatments. It was classified as a DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and contained a glutamine-rich region near its N terminus, we named it QRAP2 (for glutamine-rich AP2). Gel retardation assay revealed that QRAP2 was able to form a specific complex with the previously characterized DRE element while did not show any affinity to the GCC box or the mutant DRE box. When fused to the GAL4 DNA-binding domain, either fulllength QRAP2 or its N-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay with its C-terminus completely inactive. Our data indicate that QRAP2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream target genes in response to environmental stress, especially under drought conditions. QRAP2 (dpeaa)DE-He213 stress (dpeaa)DE-He213 DREB (dpeaa)DE-He213 one-hybrid (dpeaa)DE-He213 transcription factor (dpeaa)DE-He213 Juan, Lei verfasserin aut Wei, Gong verfasserin aut Yuxian, Zhu verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 50(2005), 17 vom: Sept., Seite 1874-1879 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:50 year:2005 number:17 month:09 pages:1874-1879 https://dx.doi.org/10.1360/982005-706 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_40 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_110 GBV_ILN_120 GBV_ILN_161 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 50 2005 17 09 1874-1879
allfieldsSound	10.1360/982005-706 doi (DE-627)SPR019373945 (SPR)982005-706-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Gang, Wei verfasserin aut Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drought, UV, abscisic acid, high salinity and salicylic acid treatments. It was classified as a DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and contained a glutamine-rich region near its N terminus, we named it QRAP2 (for glutamine-rich AP2). Gel retardation assay revealed that QRAP2 was able to form a specific complex with the previously characterized DRE element while did not show any affinity to the GCC box or the mutant DRE box. When fused to the GAL4 DNA-binding domain, either fulllength QRAP2 or its N-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay with its C-terminus completely inactive. Our data indicate that QRAP2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream target genes in response to environmental stress, especially under drought conditions. QRAP2 (dpeaa)DE-He213 stress (dpeaa)DE-He213 DREB (dpeaa)DE-He213 one-hybrid (dpeaa)DE-He213 transcription factor (dpeaa)DE-He213 Juan, Lei verfasserin aut Wei, Gong verfasserin aut Yuxian, Zhu verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 50(2005), 17 vom: Sept., Seite 1874-1879 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:50 year:2005 number:17 month:09 pages:1874-1879 https://dx.doi.org/10.1360/982005-706 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_40 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_110 GBV_ILN_120 GBV_ILN_161 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 50 2005 17 09 1874-1879
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author	Gang, Wei
spellingShingle	Gang, Wei ddc 500 bkl 30.00 misc QRAP2 misc stress misc DREB misc one-hybrid misc transcription factor Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana
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title	Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana
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title_full	Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana
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title_sort	molecular cloning, expressional profiling, dna binding and trans-activation property studies of qrap2 from arabidopsis thaliana
title_auth	Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana
abstract	Abstract Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drought, UV, abscisic acid, high salinity and salicylic acid treatments. It was classified as a DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and contained a glutamine-rich region near its N terminus, we named it QRAP2 (for glutamine-rich AP2). Gel retardation assay revealed that QRAP2 was able to form a specific complex with the previously characterized DRE element while did not show any affinity to the GCC box or the mutant DRE box. When fused to the GAL4 DNA-binding domain, either fulllength QRAP2 or its N-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay with its C-terminus completely inactive. Our data indicate that QRAP2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream target genes in response to environmental stress, especially under drought conditions.
abstractGer	Abstract Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drought, UV, abscisic acid, high salinity and salicylic acid treatments. It was classified as a DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and contained a glutamine-rich region near its N terminus, we named it QRAP2 (for glutamine-rich AP2). Gel retardation assay revealed that QRAP2 was able to form a specific complex with the previously characterized DRE element while did not show any affinity to the GCC box or the mutant DRE box. When fused to the GAL4 DNA-binding domain, either fulllength QRAP2 or its N-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay with its C-terminus completely inactive. Our data indicate that QRAP2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream target genes in response to environmental stress, especially under drought conditions.
abstract_unstemmed	Abstract Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drought, UV, abscisic acid, high salinity and salicylic acid treatments. It was classified as a DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and contained a glutamine-rich region near its N terminus, we named it QRAP2 (for glutamine-rich AP2). Gel retardation assay revealed that QRAP2 was able to form a specific complex with the previously characterized DRE element while did not show any affinity to the GCC box or the mutant DRE box. When fused to the GAL4 DNA-binding domain, either fulllength QRAP2 or its N-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay with its C-terminus completely inactive. Our data indicate that QRAP2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream target genes in response to environmental stress, especially under drought conditions.
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title_short	Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana
url	https://dx.doi.org/10.1360/982005-706
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Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thaliana

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