Combinational electroporation and transplantation approach to studying gene functions in avian embryos

Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Wang, Xiaoyu [verfasserIn] Li, Yan [verfasserIn] Wang, Guang [verfasserIn] Münsterberg, Andrea [verfasserIn] Chuai, Manli [verfasserIn] Lee, KaHo Kenneth [verfasserIn] Wang, Lijing [verfasserIn] Yang, Xuesong [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	Chick embryo Electroporation Gene transfection Transplantation

Übergeordnetes Werk:	Enthalten in: Chinese science bulletin - Beijing, China : Chinese Acad. of Sciences, 1997, 59(2014), 7 vom: 29. Jan., Seite 616-624
Übergeordnetes Werk:	volume:59 ; year:2014 ; number:7 ; day:29 ; month:01 ; pages:616-624

Links:	Volltext

DOI / URN:	10.1007/s11434-013-0090-8

Katalog-ID:	SPR019534205

Internformat


LEADER	01000caa a22002652 4500
001	SPR019534205
003	DE-627
005	20220111065953.0
007	cr uuu---uuuuu
008	201006s2014 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.1007/s11434-013-0090-8 \|2 doi
035			\|a (DE-627)SPR019534205
035			\|a (SPR)s11434-013-0090-8-e
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
082	0	4	\|a 500 \|q ASE
084			\|a 30.00 \|2 bkl
100	1		\|a Wang, Xiaoyu \|e verfasserin \|4 aut
245	1	0	\|a Combinational electroporation and transplantation approach to studying gene functions in avian embryos
264		1	\|c 2014
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene.
650		4	\|a Chick embryo \|7 (dpeaa)DE-He213
650		4	\|a Electroporation \|7 (dpeaa)DE-He213
650		4	\|a Gene transfection \|7 (dpeaa)DE-He213
650		4	\|a Transplantation \|7 (dpeaa)DE-He213
700	1		\|a Li, Yan \|e verfasserin \|4 aut
700	1		\|a Wang, Guang \|e verfasserin \|4 aut
700	1		\|a Münsterberg, Andrea \|e verfasserin \|4 aut
700	1		\|a Chuai, Manli \|e verfasserin \|4 aut
700	1		\|a Lee, KaHo Kenneth \|e verfasserin \|4 aut
700	1		\|a Wang, Lijing \|e verfasserin \|4 aut
700	1		\|a Yang, Xuesong \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Chinese science bulletin \|d Beijing, China : Chinese Acad. of Sciences, 1997 \|g 59(2014), 7 vom: 29. Jan., Seite 616-624 \|w (DE-627)341897809 \|w (DE-600)2069521-4 \|x 1861-9541 \|7 nnns
773	1	8	\|g volume:59 \|g year:2014 \|g number:7 \|g day:29 \|g month:01 \|g pages:616-624
856	4	0	\|u https://dx.doi.org/10.1007/s11434-013-0090-8 \|z lizenzpflichtig \|3 Volltext
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_SPRINGER
912			\|a GBV_ILN_70
912			\|a GBV_ILN_95
912			\|a GBV_ILN_266
912			\|a GBV_ILN_285
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2018
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4328
912			\|a GBV_ILN_4333
936	b	k	\|a 30.00 \|q ASE
951			\|a AR
952			\|d 59 \|j 2014 \|e 7 \|b 29 \|c 01 \|h 616-624

Indexfelder

author_variant	x w xw y l yl g w gw a m am m c mc k k l kk kkl l w lw x y xy
matchkey_str	article:18619541:2014----::obntoaeetooainntaslnainprahotdige
hierarchy_sort_str	2014
bklnumber	30.00
publishDate	2014
allfields	10.1007/s11434-013-0090-8 doi (DE-627)SPR019534205 (SPR)s11434-013-0090-8-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Wang, Xiaoyu verfasserin aut Combinational electroporation and transplantation approach to studying gene functions in avian embryos 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene. Chick embryo (dpeaa)DE-He213 Electroporation (dpeaa)DE-He213 Gene transfection (dpeaa)DE-He213 Transplantation (dpeaa)DE-He213 Li, Yan verfasserin aut Wang, Guang verfasserin aut Münsterberg, Andrea verfasserin aut Chuai, Manli verfasserin aut Lee, KaHo Kenneth verfasserin aut Wang, Lijing verfasserin aut Yang, Xuesong verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 59(2014), 7 vom: 29. Jan., Seite 616-624 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:59 year:2014 number:7 day:29 month:01 pages:616-624 https://dx.doi.org/10.1007/s11434-013-0090-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_70 GBV_ILN_95 GBV_ILN_266 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 59 2014 7 29 01 616-624
spelling	10.1007/s11434-013-0090-8 doi (DE-627)SPR019534205 (SPR)s11434-013-0090-8-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Wang, Xiaoyu verfasserin aut Combinational electroporation and transplantation approach to studying gene functions in avian embryos 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene. Chick embryo (dpeaa)DE-He213 Electroporation (dpeaa)DE-He213 Gene transfection (dpeaa)DE-He213 Transplantation (dpeaa)DE-He213 Li, Yan verfasserin aut Wang, Guang verfasserin aut Münsterberg, Andrea verfasserin aut Chuai, Manli verfasserin aut Lee, KaHo Kenneth verfasserin aut Wang, Lijing verfasserin aut Yang, Xuesong verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 59(2014), 7 vom: 29. Jan., Seite 616-624 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:59 year:2014 number:7 day:29 month:01 pages:616-624 https://dx.doi.org/10.1007/s11434-013-0090-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_70 GBV_ILN_95 GBV_ILN_266 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 59 2014 7 29 01 616-624
allfields_unstemmed	10.1007/s11434-013-0090-8 doi (DE-627)SPR019534205 (SPR)s11434-013-0090-8-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Wang, Xiaoyu verfasserin aut Combinational electroporation and transplantation approach to studying gene functions in avian embryos 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene. Chick embryo (dpeaa)DE-He213 Electroporation (dpeaa)DE-He213 Gene transfection (dpeaa)DE-He213 Transplantation (dpeaa)DE-He213 Li, Yan verfasserin aut Wang, Guang verfasserin aut Münsterberg, Andrea verfasserin aut Chuai, Manli verfasserin aut Lee, KaHo Kenneth verfasserin aut Wang, Lijing verfasserin aut Yang, Xuesong verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 59(2014), 7 vom: 29. Jan., Seite 616-624 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:59 year:2014 number:7 day:29 month:01 pages:616-624 https://dx.doi.org/10.1007/s11434-013-0090-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_70 GBV_ILN_95 GBV_ILN_266 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 59 2014 7 29 01 616-624
allfieldsGer	10.1007/s11434-013-0090-8 doi (DE-627)SPR019534205 (SPR)s11434-013-0090-8-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Wang, Xiaoyu verfasserin aut Combinational electroporation and transplantation approach to studying gene functions in avian embryos 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene. Chick embryo (dpeaa)DE-He213 Electroporation (dpeaa)DE-He213 Gene transfection (dpeaa)DE-He213 Transplantation (dpeaa)DE-He213 Li, Yan verfasserin aut Wang, Guang verfasserin aut Münsterberg, Andrea verfasserin aut Chuai, Manli verfasserin aut Lee, KaHo Kenneth verfasserin aut Wang, Lijing verfasserin aut Yang, Xuesong verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 59(2014), 7 vom: 29. Jan., Seite 616-624 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:59 year:2014 number:7 day:29 month:01 pages:616-624 https://dx.doi.org/10.1007/s11434-013-0090-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_70 GBV_ILN_95 GBV_ILN_266 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 59 2014 7 29 01 616-624
allfieldsSound	10.1007/s11434-013-0090-8 doi (DE-627)SPR019534205 (SPR)s11434-013-0090-8-e DE-627 ger DE-627 rakwb eng 500 ASE 30.00 bkl Wang, Xiaoyu verfasserin aut Combinational electroporation and transplantation approach to studying gene functions in avian embryos 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene. Chick embryo (dpeaa)DE-He213 Electroporation (dpeaa)DE-He213 Gene transfection (dpeaa)DE-He213 Transplantation (dpeaa)DE-He213 Li, Yan verfasserin aut Wang, Guang verfasserin aut Münsterberg, Andrea verfasserin aut Chuai, Manli verfasserin aut Lee, KaHo Kenneth verfasserin aut Wang, Lijing verfasserin aut Yang, Xuesong verfasserin aut Enthalten in Chinese science bulletin Beijing, China : Chinese Acad. of Sciences, 1997 59(2014), 7 vom: 29. Jan., Seite 616-624 (DE-627)341897809 (DE-600)2069521-4 1861-9541 nnns volume:59 year:2014 number:7 day:29 month:01 pages:616-624 https://dx.doi.org/10.1007/s11434-013-0090-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_70 GBV_ILN_95 GBV_ILN_266 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333 30.00 ASE AR 59 2014 7 29 01 616-624
language	English
source	Enthalten in Chinese science bulletin 59(2014), 7 vom: 29. Jan., Seite 616-624 volume:59 year:2014 number:7 day:29 month:01 pages:616-624
sourceStr	Enthalten in Chinese science bulletin 59(2014), 7 vom: 29. Jan., Seite 616-624 volume:59 year:2014 number:7 day:29 month:01 pages:616-624
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Chick embryo Electroporation Gene transfection Transplantation
dewey-raw	500
isfreeaccess_bool	false
container_title	Chinese science bulletin
authorswithroles_txt_mv	Wang, Xiaoyu @@aut@@ Li, Yan @@aut@@ Wang, Guang @@aut@@ Münsterberg, Andrea @@aut@@ Chuai, Manli @@aut@@ Lee, KaHo Kenneth @@aut@@ Wang, Lijing @@aut@@ Yang, Xuesong @@aut@@
publishDateDaySort_date	2014-01-29T00:00:00Z
hierarchy_top_id	341897809
dewey-sort	3500
id	SPR019534205
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR019534205</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20220111065953.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201006s2014 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s11434-013-0090-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR019534205</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s11434-013-0090-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">500</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">30.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Wang, Xiaoyu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Combinational electroporation and transplantation approach to studying gene functions in avian embryos</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2014</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Chick embryo</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Electroporation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gene transfection</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Transplantation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Yan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Guang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Münsterberg, Andrea</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chuai, Manli</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lee, KaHo Kenneth</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Lijing</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Xuesong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Chinese science bulletin</subfield><subfield code="d">Beijing, China : Chinese Acad. of Sciences, 1997</subfield><subfield code="g">59(2014), 7 vom: 29. Jan., Seite 616-624</subfield><subfield code="w">(DE-627)341897809</subfield><subfield code="w">(DE-600)2069521-4</subfield><subfield code="x">1861-9541</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:59</subfield><subfield code="g">year:2014</subfield><subfield code="g">number:7</subfield><subfield code="g">day:29</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:616-624</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s11434-013-0090-8</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_266</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2007</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2026</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2039</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2070</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2086</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2106</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2108</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2116</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2119</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2122</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2144</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2188</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2232</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4246</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4328</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">30.00</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">59</subfield><subfield code="j">2014</subfield><subfield code="e">7</subfield><subfield code="b">29</subfield><subfield code="c">01</subfield><subfield code="h">616-624</subfield></datafield></record></collection>
author	Wang, Xiaoyu
spellingShingle	Wang, Xiaoyu ddc 500 bkl 30.00 misc Chick embryo misc Electroporation misc Gene transfection misc Transplantation Combinational electroporation and transplantation approach to studying gene functions in avian embryos
authorStr	Wang, Xiaoyu
ppnlink_with_tag_str_mv	@@773@@(DE-627)341897809
format	electronic Article
dewey-ones	500 - Natural sciences & mathematics
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut
collection	springer
remote_str	true
illustrated	Not Illustrated
issn	1861-9541
topic_title	500 ASE 30.00 bkl Combinational electroporation and transplantation approach to studying gene functions in avian embryos Chick embryo (dpeaa)DE-He213 Electroporation (dpeaa)DE-He213 Gene transfection (dpeaa)DE-He213 Transplantation (dpeaa)DE-He213
topic	ddc 500 bkl 30.00 misc Chick embryo misc Electroporation misc Gene transfection misc Transplantation
topic_unstemmed	ddc 500 bkl 30.00 misc Chick embryo misc Electroporation misc Gene transfection misc Transplantation
topic_browse	ddc 500 bkl 30.00 misc Chick embryo misc Electroporation misc Gene transfection misc Transplantation
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Chinese science bulletin
hierarchy_parent_id	341897809
dewey-tens	500 - Science
hierarchy_top_title	Chinese science bulletin
isfreeaccess_txt	false
familylinks_str_mv	(DE-627)341897809 (DE-600)2069521-4
title	Combinational electroporation and transplantation approach to studying gene functions in avian embryos
ctrlnum	(DE-627)SPR019534205 (SPR)s11434-013-0090-8-e
title_full	Combinational electroporation and transplantation approach to studying gene functions in avian embryos
author_sort	Wang, Xiaoyu
journal	Chinese science bulletin
journalStr	Chinese science bulletin
lang_code	eng
isOA_bool	false
dewey-hundreds	500 - Science
recordtype	marc
publishDateSort	2014
contenttype_str_mv	txt
container_start_page	616
author_browse	Wang, Xiaoyu Li, Yan Wang, Guang Münsterberg, Andrea Chuai, Manli Lee, KaHo Kenneth Wang, Lijing Yang, Xuesong
container_volume	59
class	500 ASE 30.00 bkl
format_se	Elektronische Aufsätze
author-letter	Wang, Xiaoyu
doi_str_mv	10.1007/s11434-013-0090-8
dewey-full	500
author2-role	verfasserin
title_sort	combinational electroporation and transplantation approach to studying gene functions in avian embryos
title_auth	Combinational electroporation and transplantation approach to studying gene functions in avian embryos
abstract	Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene.
abstractGer	Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene.
abstract_unstemmed	Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_70 GBV_ILN_95 GBV_ILN_266 GBV_ILN_285 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4313 GBV_ILN_4328 GBV_ILN_4333
container_issue	7
title_short	Combinational electroporation and transplantation approach to studying gene functions in avian embryos
url	https://dx.doi.org/10.1007/s11434-013-0090-8
remote_bool	true
author2	Li, Yan Wang, Guang Münsterberg, Andrea Chuai, Manli Lee, KaHo Kenneth Wang, Lijing Yang, Xuesong
author2Str	Li, Yan Wang, Guang Münsterberg, Andrea Chuai, Manli Lee, KaHo Kenneth Wang, Lijing Yang, Xuesong
ppnlink	341897809
mediatype_str_mv	c
isOA_txt	false
hochschulschrift_bool	false
doi_str	10.1007/s11434-013-0090-8
up_date	2024-07-04T02:01:15.347Z
_version_	1803612035847553024
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR019534205</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20220111065953.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201006s2014 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s11434-013-0090-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR019534205</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s11434-013-0090-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">500</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">30.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Wang, Xiaoyu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Combinational electroporation and transplantation approach to studying gene functions in avian embryos</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2014</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Chick embryo</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Electroporation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gene transfection</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Transplantation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Yan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Guang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Münsterberg, Andrea</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chuai, Manli</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lee, KaHo Kenneth</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Lijing</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Xuesong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Chinese science bulletin</subfield><subfield code="d">Beijing, China : Chinese Acad. of Sciences, 1997</subfield><subfield code="g">59(2014), 7 vom: 29. Jan., Seite 616-624</subfield><subfield code="w">(DE-627)341897809</subfield><subfield code="w">(DE-600)2069521-4</subfield><subfield code="x">1861-9541</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:59</subfield><subfield code="g">year:2014</subfield><subfield code="g">number:7</subfield><subfield code="g">day:29</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:616-624</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s11434-013-0090-8</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_266</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2007</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2026</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2039</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2070</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2086</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2106</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2108</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2116</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2119</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2122</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2144</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2188</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2232</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4246</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4328</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">30.00</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">59</subfield><subfield code="j">2014</subfield><subfield code="e">7</subfield><subfield code="b">29</subfield><subfield code="c">01</subfield><subfield code="h">616-624</subfield></datafield></record></collection>
score	7.3981953

Nicht das Richtige dabei?

Schreiben Sie uns!

Combinational electroporation and transplantation approach to studying gene functions in avian embryos

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?