Analysis of hybridization in DNA microarrays: Hybridization energy isotherms
Abstract The experimental data on hybridization in DNA microarrays can be presented in form of Hybridization Energy Isotherms (HEI)—relation between the level of saturation of microarray cells with target molecules and the free energy of formation of the target-probe duplex (ΔG). The study of HEI is...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Stirmanov, Ya. V. [verfasserIn] Khodykov, M. V. [verfasserIn] Matveeva, O. V. [verfasserIn] Nechipurenko, Yu. D. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Biophysics - Moscow : Maik Nauka/Interperiodica, 1995, 58(2013), 6 vom: Nov., Seite 771-774 |
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| Übergeordnetes Werk: |
volume:58 ; year:2013 ; number:6 ; month:11 ; pages:771-774 |
| Links: |
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| DOI / URN: |
10.1134/S0006350913060183 |
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| Katalog-ID: |
SPR019637772 |
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| 100 | 1 | |a Stirmanov, Ya. V. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Analysis of hybridization in DNA microarrays: Hybridization energy isotherms |
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| 520 | |a Abstract The experimental data on hybridization in DNA microarrays can be presented in form of Hybridization Energy Isotherms (HEI)—relation between the level of saturation of microarray cells with target molecules and the free energy of formation of the target-probe duplex (ΔG). The study of HEI is important for optimization of the design of biological microarrays to expand its effective range of target concentrations. The effect of vertical shift of HEI with increase of concentration of the targets was found previously in experiment. We analyzed the behavior of HEI and introduced a model explaining the experimental data and the shift effect. | ||
| 650 | 4 | |a DNA microarray |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a theory of adsorption |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a hybridization isotherm |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a oligonucleotide probe |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a oligonucleotide target |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Khodykov, M. V. |e verfasserin |4 aut | |
| 700 | 1 | |a Matveeva, O. V. |e verfasserin |4 aut | |
| 700 | 1 | |a Nechipurenko, Yu. D. |e verfasserin |4 aut | |
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10.1134/S0006350913060183 doi (DE-627)SPR019637772 (SPR)S0006350913060183-e DE-627 ger DE-627 rakwb eng 570 530 ASE 42.12 bkl Stirmanov, Ya. V. verfasserin aut Analysis of hybridization in DNA microarrays: Hybridization energy isotherms 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The experimental data on hybridization in DNA microarrays can be presented in form of Hybridization Energy Isotherms (HEI)—relation between the level of saturation of microarray cells with target molecules and the free energy of formation of the target-probe duplex (ΔG). The study of HEI is important for optimization of the design of biological microarrays to expand its effective range of target concentrations. The effect of vertical shift of HEI with increase of concentration of the targets was found previously in experiment. We analyzed the behavior of HEI and introduced a model explaining the experimental data and the shift effect. DNA microarray (dpeaa)DE-He213 theory of adsorption (dpeaa)DE-He213 hybridization isotherm (dpeaa)DE-He213 oligonucleotide probe (dpeaa)DE-He213 oligonucleotide target (dpeaa)DE-He213 Khodykov, M. V. verfasserin aut Matveeva, O. V. verfasserin aut Nechipurenko, Yu. D. verfasserin aut Enthalten in Biophysics Moscow : Maik Nauka/Interperiodica, 1995 58(2013), 6 vom: Nov., Seite 771-774 (DE-627)32063955X (DE-600)2024886-6 1555-6654 nnns volume:58 year:2013 number:6 month:11 pages:771-774 https://dx.doi.org/10.1134/S0006350913060183 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.12 ASE AR 58 2013 6 11 771-774 |
| spelling |
10.1134/S0006350913060183 doi (DE-627)SPR019637772 (SPR)S0006350913060183-e DE-627 ger DE-627 rakwb eng 570 530 ASE 42.12 bkl Stirmanov, Ya. V. verfasserin aut Analysis of hybridization in DNA microarrays: Hybridization energy isotherms 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The experimental data on hybridization in DNA microarrays can be presented in form of Hybridization Energy Isotherms (HEI)—relation between the level of saturation of microarray cells with target molecules and the free energy of formation of the target-probe duplex (ΔG). The study of HEI is important for optimization of the design of biological microarrays to expand its effective range of target concentrations. The effect of vertical shift of HEI with increase of concentration of the targets was found previously in experiment. We analyzed the behavior of HEI and introduced a model explaining the experimental data and the shift effect. DNA microarray (dpeaa)DE-He213 theory of adsorption (dpeaa)DE-He213 hybridization isotherm (dpeaa)DE-He213 oligonucleotide probe (dpeaa)DE-He213 oligonucleotide target (dpeaa)DE-He213 Khodykov, M. V. verfasserin aut Matveeva, O. V. verfasserin aut Nechipurenko, Yu. D. verfasserin aut Enthalten in Biophysics Moscow : Maik Nauka/Interperiodica, 1995 58(2013), 6 vom: Nov., Seite 771-774 (DE-627)32063955X (DE-600)2024886-6 1555-6654 nnns volume:58 year:2013 number:6 month:11 pages:771-774 https://dx.doi.org/10.1134/S0006350913060183 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.12 ASE AR 58 2013 6 11 771-774 |
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10.1134/S0006350913060183 doi (DE-627)SPR019637772 (SPR)S0006350913060183-e DE-627 ger DE-627 rakwb eng 570 530 ASE 42.12 bkl Stirmanov, Ya. V. verfasserin aut Analysis of hybridization in DNA microarrays: Hybridization energy isotherms 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The experimental data on hybridization in DNA microarrays can be presented in form of Hybridization Energy Isotherms (HEI)—relation between the level of saturation of microarray cells with target molecules and the free energy of formation of the target-probe duplex (ΔG). The study of HEI is important for optimization of the design of biological microarrays to expand its effective range of target concentrations. The effect of vertical shift of HEI with increase of concentration of the targets was found previously in experiment. We analyzed the behavior of HEI and introduced a model explaining the experimental data and the shift effect. DNA microarray (dpeaa)DE-He213 theory of adsorption (dpeaa)DE-He213 hybridization isotherm (dpeaa)DE-He213 oligonucleotide probe (dpeaa)DE-He213 oligonucleotide target (dpeaa)DE-He213 Khodykov, M. V. verfasserin aut Matveeva, O. V. verfasserin aut Nechipurenko, Yu. D. verfasserin aut Enthalten in Biophysics Moscow : Maik Nauka/Interperiodica, 1995 58(2013), 6 vom: Nov., Seite 771-774 (DE-627)32063955X (DE-600)2024886-6 1555-6654 nnns volume:58 year:2013 number:6 month:11 pages:771-774 https://dx.doi.org/10.1134/S0006350913060183 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.12 ASE AR 58 2013 6 11 771-774 |
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10.1134/S0006350913060183 doi (DE-627)SPR019637772 (SPR)S0006350913060183-e DE-627 ger DE-627 rakwb eng 570 530 ASE 42.12 bkl Stirmanov, Ya. V. verfasserin aut Analysis of hybridization in DNA microarrays: Hybridization energy isotherms 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The experimental data on hybridization in DNA microarrays can be presented in form of Hybridization Energy Isotherms (HEI)—relation between the level of saturation of microarray cells with target molecules and the free energy of formation of the target-probe duplex (ΔG). The study of HEI is important for optimization of the design of biological microarrays to expand its effective range of target concentrations. The effect of vertical shift of HEI with increase of concentration of the targets was found previously in experiment. We analyzed the behavior of HEI and introduced a model explaining the experimental data and the shift effect. DNA microarray (dpeaa)DE-He213 theory of adsorption (dpeaa)DE-He213 hybridization isotherm (dpeaa)DE-He213 oligonucleotide probe (dpeaa)DE-He213 oligonucleotide target (dpeaa)DE-He213 Khodykov, M. V. verfasserin aut Matveeva, O. V. verfasserin aut Nechipurenko, Yu. D. verfasserin aut Enthalten in Biophysics Moscow : Maik Nauka/Interperiodica, 1995 58(2013), 6 vom: Nov., Seite 771-774 (DE-627)32063955X (DE-600)2024886-6 1555-6654 nnns volume:58 year:2013 number:6 month:11 pages:771-774 https://dx.doi.org/10.1134/S0006350913060183 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.12 ASE AR 58 2013 6 11 771-774 |
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Stirmanov, Ya. V. |
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Stirmanov, Ya. V. ddc 570 bkl 42.12 misc DNA microarray misc theory of adsorption misc hybridization isotherm misc oligonucleotide probe misc oligonucleotide target Analysis of hybridization in DNA microarrays: Hybridization energy isotherms |
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570 530 ASE 42.12 bkl Analysis of hybridization in DNA microarrays: Hybridization energy isotherms DNA microarray (dpeaa)DE-He213 theory of adsorption (dpeaa)DE-He213 hybridization isotherm (dpeaa)DE-He213 oligonucleotide probe (dpeaa)DE-He213 oligonucleotide target (dpeaa)DE-He213 |
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analysis of hybridization in dna microarrays: hybridization energy isotherms |
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Analysis of hybridization in DNA microarrays: Hybridization energy isotherms |
| abstract |
Abstract The experimental data on hybridization in DNA microarrays can be presented in form of Hybridization Energy Isotherms (HEI)—relation between the level of saturation of microarray cells with target molecules and the free energy of formation of the target-probe duplex (ΔG). The study of HEI is important for optimization of the design of biological microarrays to expand its effective range of target concentrations. The effect of vertical shift of HEI with increase of concentration of the targets was found previously in experiment. We analyzed the behavior of HEI and introduced a model explaining the experimental data and the shift effect. |
| abstractGer |
Abstract The experimental data on hybridization in DNA microarrays can be presented in form of Hybridization Energy Isotherms (HEI)—relation between the level of saturation of microarray cells with target molecules and the free energy of formation of the target-probe duplex (ΔG). The study of HEI is important for optimization of the design of biological microarrays to expand its effective range of target concentrations. The effect of vertical shift of HEI with increase of concentration of the targets was found previously in experiment. We analyzed the behavior of HEI and introduced a model explaining the experimental data and the shift effect. |
| abstract_unstemmed |
Abstract The experimental data on hybridization in DNA microarrays can be presented in form of Hybridization Energy Isotherms (HEI)—relation between the level of saturation of microarray cells with target molecules and the free energy of formation of the target-probe duplex (ΔG). The study of HEI is important for optimization of the design of biological microarrays to expand its effective range of target concentrations. The effect of vertical shift of HEI with increase of concentration of the targets was found previously in experiment. We analyzed the behavior of HEI and introduced a model explaining the experimental data and the shift effect. |
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Analysis of hybridization in DNA microarrays: Hybridization energy isotherms |
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2024-07-04T02:24:26.221Z |
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