Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function

Background Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r...
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Gespeichert in:

Autor*in:	Tariq, Amna R. [verfasserIn] Shahab, Muhammad Clarke, Iain J. Pereira, Alda Smith, Jeremy T. Khan, Saeed-ul-Hasan Sultan, Javeria Javed, Sehrish Anwar, Tayyaba

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	Immunohistochemistry Spermatogenesis Germ cells Sertoli cells

Anmerkung:	© Versita Warsaw and Springer-Verlag Wien 2013

Übergeordnetes Werk:	Enthalten in: Central European journal of biology - Warsaw : Central European Science Journals, 2006, 8(2013), 10 vom: 02. Aug., Seite 968-974
Übergeordnetes Werk:	volume:8 ; year:2013 ; number:10 ; day:02 ; month:08 ; pages:968-974

Links:	Volltext

DOI / URN:	10.2478/s11535-013-0219-4

Katalog-ID:	SPR020827822

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520			\|a Background Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. Kiss1-Kiss1r in testis suggests possible direct involvement in the regulatory network involved in spermatogenesis.
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allfields	10.2478/s11535-013-0219-4 doi (DE-627)SPR020827822 (SPR)s11535-013-0219-4-e DE-627 ger DE-627 rakwb eng Tariq, Amna R. verfasserin aut Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Versita Warsaw and Springer-Verlag Wien 2013 Background Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. Kiss1-Kiss1r in testis suggests possible direct involvement in the regulatory network involved in spermatogenesis. Immunohistochemistry (dpeaa)DE-He213 Spermatogenesis (dpeaa)DE-He213 Germ cells (dpeaa)DE-He213 Sertoli cells (dpeaa)DE-He213 Shahab, Muhammad aut Clarke, Iain J. aut Pereira, Alda aut Smith, Jeremy T. aut Khan, Saeed-ul-Hasan aut Sultan, Javeria aut Javed, Sehrish aut Anwar, Tayyaba aut Enthalten in Central European journal of biology Warsaw : Central European Science Journals, 2006 8(2013), 10 vom: 02. Aug., Seite 968-974 (DE-627)511641087 (DE-600)2234099-3 1644-3632 nnns volume:8 year:2013 number:10 day:02 month:08 pages:968-974 https://dx.doi.org/10.2478/s11535-013-0219-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2013 10 02 08 968-974
spelling	10.2478/s11535-013-0219-4 doi (DE-627)SPR020827822 (SPR)s11535-013-0219-4-e DE-627 ger DE-627 rakwb eng Tariq, Amna R. verfasserin aut Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Versita Warsaw and Springer-Verlag Wien 2013 Background Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. Kiss1-Kiss1r in testis suggests possible direct involvement in the regulatory network involved in spermatogenesis. Immunohistochemistry (dpeaa)DE-He213 Spermatogenesis (dpeaa)DE-He213 Germ cells (dpeaa)DE-He213 Sertoli cells (dpeaa)DE-He213 Shahab, Muhammad aut Clarke, Iain J. aut Pereira, Alda aut Smith, Jeremy T. aut Khan, Saeed-ul-Hasan aut Sultan, Javeria aut Javed, Sehrish aut Anwar, Tayyaba aut Enthalten in Central European journal of biology Warsaw : Central European Science Journals, 2006 8(2013), 10 vom: 02. Aug., Seite 968-974 (DE-627)511641087 (DE-600)2234099-3 1644-3632 nnns volume:8 year:2013 number:10 day:02 month:08 pages:968-974 https://dx.doi.org/10.2478/s11535-013-0219-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2013 10 02 08 968-974
allfields_unstemmed	10.2478/s11535-013-0219-4 doi (DE-627)SPR020827822 (SPR)s11535-013-0219-4-e DE-627 ger DE-627 rakwb eng Tariq, Amna R. verfasserin aut Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Versita Warsaw and Springer-Verlag Wien 2013 Background Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. Kiss1-Kiss1r in testis suggests possible direct involvement in the regulatory network involved in spermatogenesis. Immunohistochemistry (dpeaa)DE-He213 Spermatogenesis (dpeaa)DE-He213 Germ cells (dpeaa)DE-He213 Sertoli cells (dpeaa)DE-He213 Shahab, Muhammad aut Clarke, Iain J. aut Pereira, Alda aut Smith, Jeremy T. aut Khan, Saeed-ul-Hasan aut Sultan, Javeria aut Javed, Sehrish aut Anwar, Tayyaba aut Enthalten in Central European journal of biology Warsaw : Central European Science Journals, 2006 8(2013), 10 vom: 02. Aug., Seite 968-974 (DE-627)511641087 (DE-600)2234099-3 1644-3632 nnns volume:8 year:2013 number:10 day:02 month:08 pages:968-974 https://dx.doi.org/10.2478/s11535-013-0219-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2013 10 02 08 968-974
allfieldsGer	10.2478/s11535-013-0219-4 doi (DE-627)SPR020827822 (SPR)s11535-013-0219-4-e DE-627 ger DE-627 rakwb eng Tariq, Amna R. verfasserin aut Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Versita Warsaw and Springer-Verlag Wien 2013 Background Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. Kiss1-Kiss1r in testis suggests possible direct involvement in the regulatory network involved in spermatogenesis. Immunohistochemistry (dpeaa)DE-He213 Spermatogenesis (dpeaa)DE-He213 Germ cells (dpeaa)DE-He213 Sertoli cells (dpeaa)DE-He213 Shahab, Muhammad aut Clarke, Iain J. aut Pereira, Alda aut Smith, Jeremy T. aut Khan, Saeed-ul-Hasan aut Sultan, Javeria aut Javed, Sehrish aut Anwar, Tayyaba aut Enthalten in Central European journal of biology Warsaw : Central European Science Journals, 2006 8(2013), 10 vom: 02. Aug., Seite 968-974 (DE-627)511641087 (DE-600)2234099-3 1644-3632 nnns volume:8 year:2013 number:10 day:02 month:08 pages:968-974 https://dx.doi.org/10.2478/s11535-013-0219-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2013 10 02 08 968-974
allfieldsSound	10.2478/s11535-013-0219-4 doi (DE-627)SPR020827822 (SPR)s11535-013-0219-4-e DE-627 ger DE-627 rakwb eng Tariq, Amna R. verfasserin aut Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Versita Warsaw and Springer-Verlag Wien 2013 Background Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. Kiss1-Kiss1r in testis suggests possible direct involvement in the regulatory network involved in spermatogenesis. Immunohistochemistry (dpeaa)DE-He213 Spermatogenesis (dpeaa)DE-He213 Germ cells (dpeaa)DE-He213 Sertoli cells (dpeaa)DE-He213 Shahab, Muhammad aut Clarke, Iain J. aut Pereira, Alda aut Smith, Jeremy T. aut Khan, Saeed-ul-Hasan aut Sultan, Javeria aut Javed, Sehrish aut Anwar, Tayyaba aut Enthalten in Central European journal of biology Warsaw : Central European Science Journals, 2006 8(2013), 10 vom: 02. Aug., Seite 968-974 (DE-627)511641087 (DE-600)2234099-3 1644-3632 nnns volume:8 year:2013 number:10 day:02 month:08 pages:968-974 https://dx.doi.org/10.2478/s11535-013-0219-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2013 10 02 08 968-974
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We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. 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author	Tariq, Amna R.
spellingShingle	Tariq, Amna R. misc Immunohistochemistry misc Spermatogenesis misc Germ cells misc Sertoli cells Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function
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topic_title	Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function Immunohistochemistry (dpeaa)DE-He213 Spermatogenesis (dpeaa)DE-He213 Germ cells (dpeaa)DE-He213 Sertoli cells (dpeaa)DE-He213
topic	misc Immunohistochemistry misc Spermatogenesis misc Germ cells misc Sertoli cells
topic_unstemmed	misc Immunohistochemistry misc Spermatogenesis misc Germ cells misc Sertoli cells
topic_browse	misc Immunohistochemistry misc Spermatogenesis misc Germ cells misc Sertoli cells
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title	Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function
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title_full	Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function
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author_browse	Tariq, Amna R. Shahab, Muhammad Clarke, Iain J. Pereira, Alda Smith, Jeremy T. Khan, Saeed-ul-Hasan Sultan, Javeria Javed, Sehrish Anwar, Tayyaba
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title_sort	kiss1 and kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function
title_auth	Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function
abstract	Background Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. Kiss1-Kiss1r in testis suggests possible direct involvement in the regulatory network involved in spermatogenesis. © Versita Warsaw and Springer-Verlag Wien 2013
abstractGer	Background Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. Kiss1-Kiss1r in testis suggests possible direct involvement in the regulatory network involved in spermatogenesis. © Versita Warsaw and Springer-Verlag Wien 2013
abstract_unstemmed	Background Kisspeptin, a KISS1 gene product, stimulates GnRH neurons in the hypothalamus, but some recent studies have also suggested a direct effect on gonads. We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. Kiss1-Kiss1r in testis suggests possible direct involvement in the regulatory network involved in spermatogenesis. © Versita Warsaw and Springer-Verlag Wien 2013
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title_short	Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function
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author2	Shahab, Muhammad Clarke, Iain J. Pereira, Alda Smith, Jeremy T. Khan, Saeed-ul-Hasan Sultan, Javeria Javed, Sehrish Anwar, Tayyaba
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up_date	2024-07-03T18:30:36.590Z
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We aimed to localise Kiss and Kiss1 receptor (Kiss1r) in adult rhesus monkey testis. Experimental Procedures Expression of Kiss1 and Kiss1r was detected in testicular tissue of rhesus monkey using immunohistochemistry and reverse transcriptase PCR (RT-PCR). Dual immunohistochemistry was used to colocalize Kiss1 with germ cell marker T4 and Kiss1r with inhibin (Sertoli cell marker) using specific antibodies for all. Immunohistochemistry of testis was conducted on formaldehyde fixed tissues using 5 µ thick sections. Using gene specific primers, RT-PCR was carried out to find expression of Kiss1 and Kiss1r mRNAs in testis. Results Kiss1 immunoreactivity was localised to spermatocytes and spermatids and Kiss1r was observed in spermatocytes and Sertoli cells. Double-label immunohistochemistry co-localized Kiss1 and T4 in spermatids and Kiss1r and inhibin were co-localized in Sertoli cells. RT-PCR showed mRNA expression of Kiss1 and Kiss1r in adult rhesus monkey testis. Conclusions Present results indicate for the first time the presence of Kiss1 and Kiss1r in adult primate testis. These suggest a possible autocrine/paracrine role of kisspeptin in non-human primate testis. 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Kiss1 and Kiss1 receptor expression in the rhesus monkey testis: a possible local regulator of testicular function

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