Comparative study of the antioxidant activity of some thiol-containing substances

Background Therapeutic thiol administration has been shown to have great potential in a variety of pathological conditions associated with oxidative stress. In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopri...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Petrov, Lubomir [verfasserIn] Atanassova, Mila Alexandrova, Albena

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Schlagwörter:	Captopril Cysteamine Hydroxyl radical Mercaptoethanol Superoxide anion radical

Anmerkung:	© © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011

Übergeordnetes Werk:	Enthalten in: Central European journal of medicine - Warsaw : Central European Science Journals, 2006, 7(2011), 2 vom: 29. Dez., Seite 269-273
Übergeordnetes Werk:	volume:7 ; year:2011 ; number:2 ; day:29 ; month:12 ; pages:269-273

Links:	Volltext

DOI / URN:	10.2478/s11536-011-0132-z

Katalog-ID:	SPR020903243

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245	1	0	\|a Comparative study of the antioxidant activity of some thiol-containing substances
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520			\|a Background Therapeutic thiol administration has been shown to have great potential in a variety of pathological conditions associated with oxidative stress. In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH.
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allfields	10.2478/s11536-011-0132-z doi (DE-627)SPR020903243 (SPR)s11536-011-0132-z-e DE-627 ger DE-627 rakwb eng Petrov, Lubomir verfasserin aut Comparative study of the antioxidant activity of some thiol-containing substances 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011 Background Therapeutic thiol administration has been shown to have great potential in a variety of pathological conditions associated with oxidative stress. In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH. Captopril (dpeaa)DE-He213 Cysteamine (dpeaa)DE-He213 Hydroxyl radical (dpeaa)DE-He213 Mercaptoethanol (dpeaa)DE-He213 Superoxide anion radical (dpeaa)DE-He213 Atanassova, Mila aut Alexandrova, Albena aut Enthalten in Central European journal of medicine Warsaw : Central European Science Journals, 2006 7(2011), 2 vom: 29. Dez., Seite 269-273 (DE-627)511914962 (DE-600)2234698-3 1644-3640 nnns volume:7 year:2011 number:2 day:29 month:12 pages:269-273 https://dx.doi.org/10.2478/s11536-011-0132-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2011 2 29 12 269-273
spelling	10.2478/s11536-011-0132-z doi (DE-627)SPR020903243 (SPR)s11536-011-0132-z-e DE-627 ger DE-627 rakwb eng Petrov, Lubomir verfasserin aut Comparative study of the antioxidant activity of some thiol-containing substances 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011 Background Therapeutic thiol administration has been shown to have great potential in a variety of pathological conditions associated with oxidative stress. In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH. Captopril (dpeaa)DE-He213 Cysteamine (dpeaa)DE-He213 Hydroxyl radical (dpeaa)DE-He213 Mercaptoethanol (dpeaa)DE-He213 Superoxide anion radical (dpeaa)DE-He213 Atanassova, Mila aut Alexandrova, Albena aut Enthalten in Central European journal of medicine Warsaw : Central European Science Journals, 2006 7(2011), 2 vom: 29. Dez., Seite 269-273 (DE-627)511914962 (DE-600)2234698-3 1644-3640 nnns volume:7 year:2011 number:2 day:29 month:12 pages:269-273 https://dx.doi.org/10.2478/s11536-011-0132-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2011 2 29 12 269-273
allfields_unstemmed	10.2478/s11536-011-0132-z doi (DE-627)SPR020903243 (SPR)s11536-011-0132-z-e DE-627 ger DE-627 rakwb eng Petrov, Lubomir verfasserin aut Comparative study of the antioxidant activity of some thiol-containing substances 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011 Background Therapeutic thiol administration has been shown to have great potential in a variety of pathological conditions associated with oxidative stress. In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH. Captopril (dpeaa)DE-He213 Cysteamine (dpeaa)DE-He213 Hydroxyl radical (dpeaa)DE-He213 Mercaptoethanol (dpeaa)DE-He213 Superoxide anion radical (dpeaa)DE-He213 Atanassova, Mila aut Alexandrova, Albena aut Enthalten in Central European journal of medicine Warsaw : Central European Science Journals, 2006 7(2011), 2 vom: 29. Dez., Seite 269-273 (DE-627)511914962 (DE-600)2234698-3 1644-3640 nnns volume:7 year:2011 number:2 day:29 month:12 pages:269-273 https://dx.doi.org/10.2478/s11536-011-0132-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2011 2 29 12 269-273
allfieldsGer	10.2478/s11536-011-0132-z doi (DE-627)SPR020903243 (SPR)s11536-011-0132-z-e DE-627 ger DE-627 rakwb eng Petrov, Lubomir verfasserin aut Comparative study of the antioxidant activity of some thiol-containing substances 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011 Background Therapeutic thiol administration has been shown to have great potential in a variety of pathological conditions associated with oxidative stress. In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH. Captopril (dpeaa)DE-He213 Cysteamine (dpeaa)DE-He213 Hydroxyl radical (dpeaa)DE-He213 Mercaptoethanol (dpeaa)DE-He213 Superoxide anion radical (dpeaa)DE-He213 Atanassova, Mila aut Alexandrova, Albena aut Enthalten in Central European journal of medicine Warsaw : Central European Science Journals, 2006 7(2011), 2 vom: 29. Dez., Seite 269-273 (DE-627)511914962 (DE-600)2234698-3 1644-3640 nnns volume:7 year:2011 number:2 day:29 month:12 pages:269-273 https://dx.doi.org/10.2478/s11536-011-0132-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2011 2 29 12 269-273
allfieldsSound	10.2478/s11536-011-0132-z doi (DE-627)SPR020903243 (SPR)s11536-011-0132-z-e DE-627 ger DE-627 rakwb eng Petrov, Lubomir verfasserin aut Comparative study of the antioxidant activity of some thiol-containing substances 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011 Background Therapeutic thiol administration has been shown to have great potential in a variety of pathological conditions associated with oxidative stress. In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH. Captopril (dpeaa)DE-He213 Cysteamine (dpeaa)DE-He213 Hydroxyl radical (dpeaa)DE-He213 Mercaptoethanol (dpeaa)DE-He213 Superoxide anion radical (dpeaa)DE-He213 Atanassova, Mila aut Alexandrova, Albena aut Enthalten in Central European journal of medicine Warsaw : Central European Science Journals, 2006 7(2011), 2 vom: 29. Dez., Seite 269-273 (DE-627)511914962 (DE-600)2234698-3 1644-3640 nnns volume:7 year:2011 number:2 day:29 month:12 pages:269-273 https://dx.doi.org/10.2478/s11536-011-0132-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2011 2 29 12 269-273
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In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Captopril</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cysteamine</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hydroxyl radical</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mercaptoethanol</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Superoxide anion radical</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Atanassova, Mila</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Alexandrova, Albena</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Central European journal of medicine</subfield><subfield code="d">Warsaw : Central European Science Journals, 2006</subfield><subfield code="g">7(2011), 2 vom: 29. 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author	Petrov, Lubomir
spellingShingle	Petrov, Lubomir misc Captopril misc Cysteamine misc Hydroxyl radical misc Mercaptoethanol misc Superoxide anion radical Comparative study of the antioxidant activity of some thiol-containing substances
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topic_title	Comparative study of the antioxidant activity of some thiol-containing substances Captopril (dpeaa)DE-He213 Cysteamine (dpeaa)DE-He213 Hydroxyl radical (dpeaa)DE-He213 Mercaptoethanol (dpeaa)DE-He213 Superoxide anion radical (dpeaa)DE-He213
topic	misc Captopril misc Cysteamine misc Hydroxyl radical misc Mercaptoethanol misc Superoxide anion radical
topic_unstemmed	misc Captopril misc Cysteamine misc Hydroxyl radical misc Mercaptoethanol misc Superoxide anion radical
topic_browse	misc Captopril misc Cysteamine misc Hydroxyl radical misc Mercaptoethanol misc Superoxide anion radical
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title	Comparative study of the antioxidant activity of some thiol-containing substances
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title_full	Comparative study of the antioxidant activity of some thiol-containing substances
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author_browse	Petrov, Lubomir Atanassova, Mila Alexandrova, Albena
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title_sort	comparative study of the antioxidant activity of some thiol-containing substances
title_auth	Comparative study of the antioxidant activity of some thiol-containing substances
abstract	Background Therapeutic thiol administration has been shown to have great potential in a variety of pathological conditions associated with oxidative stress. In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH. © © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011
abstractGer	Background Therapeutic thiol administration has been shown to have great potential in a variety of pathological conditions associated with oxidative stress. In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH. © © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011
abstract_unstemmed	Background Therapeutic thiol administration has been shown to have great potential in a variety of pathological conditions associated with oxidative stress. In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH. © © Versita Warsaw and Springer-Verlag Berlin Heidelberg 2011
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title_short	Comparative study of the antioxidant activity of some thiol-containing substances
url	https://dx.doi.org/10.2478/s11536-011-0132-z
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author2	Atanassova, Mila Alexandrova, Albena
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up_date	2024-07-03T19:00:41.238Z
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In the present study, the free radical scavenging effects against superoxide anion (${\rm O}_{2^ - } $) and hydroxyl (·OH) radicals of captopril were compared with those of cysteamine and mercaptoethanol. Methods: The ${\rm O}_{2^ - } $ and ·OH were generated in vitro. Deoxyribose (DR) was used as a detector of ·OH radicals. The degradation of DR was measured in terms of the formation of thiobarbituric acid reactive substances, which were quantified spectrophotometrically. Superoxide anion radicals were generated photochemically and ${\rm O}_{2^ - } $-produced nitro-blue tetrazolium (NBT) reduction was measured. Results Using two distinct ·OH generating systems, the DR test showed that in the absence of the chelator diethylene triamine pentaacetic acid (DTPA) cysteamine was much more potent inhibitor of the formation of thiobarbituric acid reactive substances (TBARs) than captopril and mercaptoethanol, and that in the presence of DTPA captopril and mercaptoethanol decreased the TBARs formation in presence of $ H_{2} %$ O_{2} $ better than cysteamine. Captopril in concentration of 9.34 mM and cysteamine in concentration of 1.21 mM inhibited the ${\rm O}_{2^ - } $ provoked NBT reduction by 50%. Mercaptoethanol up to 10 mM did not manifest an inhibitory effect. Conclusions Captopril and mercaptoethanol are potent free radical scavengers, reacting rapidly with ·OH, whereas cysteamine acts preferentially as a chelator of iron and in this way prevents the formation of ·OH.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Captopril</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cysteamine</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hydroxyl radical</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mercaptoethanol</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Superoxide anion radical</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Atanassova, Mila</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Alexandrova, Albena</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Central European journal of medicine</subfield><subfield code="d">Warsaw : Central European Science Journals, 2006</subfield><subfield code="g">7(2011), 2 vom: 29. 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Comparative study of the antioxidant activity of some thiol-containing substances

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